家禽养殖场环境分离肠沙门氏菌1,4,bb0,12,12:i:-的分子特征和耐药性

IF 3 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY
Xiaojie Qin, Linlin Xiao, Jiaming Li, Mingzhe Yang, Changying Yang, Qingli Dong
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引用次数: 1

摘要

肠炎沙门氏菌血清型1,4,[5],12:i:-(S.1,4,[5],12:i:-)近年来被认为是一种新出现的食源性病原体。它会导致人类沙门氏菌病,主要是由生家禽和猪肉等动物源性食品污染引起的。本研究旨在利用脉冲场凝胶电泳(PFGE)、多点序列分型(MLST)、基于聚合酶链式反应的复制子分型和最小抑制浓度法,对从两个家禽养殖场采集的15个S.1,4,[5],12:i:-环境分离株的遗传多样性、质粒复制子类型和抗生素耐药性进行表征。检测到10种不同的PFGE基因型,表明这些S.1,4,[5],12:i:-分离株之间具有高度多样性。MLST鉴定出三种序列类型(ST19、ST1544、ST34)。其中,ST1544最早在来自家禽养殖场的S.1,4,[5],12:i:-环境分离株中检测到。所有分离株均对头孢唑林、头孢替坦、妥布霉素、阿米卡星和庆大霉素耐药,但对哌拉西林-他唑巴坦、氨曲南、头孢他啶、头孢吡肟和厄他培南敏感。发现5个质粒不亲和群(Inc),包括IncFII(66.7%)、IncHI2(20%)、IncI1(6.7%)、IncN(6.7%,)和IncQ(6.7%。有趣的是,多重耐药分离株263携带许多抗性基因(即qnrS、aac(6’)-Ib-cr、blaTEM、blaCTX-M-9、blaOXA-1、sul1、sul2、sul3、floR和mcr-1)和i类整合子酶基因intI1,其同时具有IncHI2和IncQ质粒,这表明抗性基因可以通过IncHI2质粒和IncQ载体的组合水平转移。总的来说,抗生素耐药性S.1,4,[5],12:i:-分离株首次在中国家禽养殖场环境中发现,应加强监测,防止其从家禽养殖场进一步传播到食品中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular characterization and antibiotic resistance of Salmonella enterica serovar 1,4,[5],12:i:- environmental isolates from poultry farms
Salmonella enterica serovar 1,4,[5],12:i:- (S. 1,4,[5],12:i:-) has been recognized as an emerging foodborne pathogen in recent years. It can cause human salmonellosis predominated by the contamination of animal-derived foods such as raw poultry and pork. This study aimed to characterize the genetic diversity, plasmid replicon types, and antibiotic resistance of 15 S. 1,4,[5],12:i:- environmental isolates collected from two poultry farms using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), polymerase chain reaction-based replicon typing, and minimum inhibitory concentration approach. Ten different PFGE genotypes were detected, indicating a high diversity among these S. 1,4,[5],12:i:- isolates. Three sequence types (ST19, ST1544, ST34) were identified by MLST. Among them, ST1544 was first detected in S. 1,4,[5],12:i:- environmental isolates from poultry farms. All isolates were resistant to cefazolin, cefotetan, tobramycin, amikacin, and gentamicin, but susceptible to piperacillin-tazobactam, aztreonam, ceftazidime, cefepime, and ertapenem. Five incompatibility groups (Inc) of plasmids were identified, including IncFIIs (66.7%), IncHI2 (20%), IncI1 (6.7%), IncN (6.7%), and IncQ (6.7%). Among these, 80% of isolates carried at least one plasmid replicon type, and 20% of isolates carried multiple plasmid replicon types. Interestingly, the multidrug-resistant isolate 263 carried numerous resistance genes (i.e., qnrS, aac(6’)-Ib-cr, blaTEM, blaCTX-M-9, blaOXA-1, sul1, sul2, sul3, floR, and mcr-1) and class I integronase gene intI1, which possessed both IncHI2 and IncQ plasmids, suggesting that resistance genes may be horizontally transferred by the combination of IncHI2 and IncQ plasmids. Collectively, antibiotic-resistant S.1,4,[5],12:i:- isolates were first found in poultry farm environments in China, and its surveillance should be strengthened to prevent its further spread from poultry farms to foods.
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来源期刊
Food Quality and Safety
Food Quality and Safety FOOD SCIENCE & TECHNOLOGY-
CiteScore
7.20
自引率
1.80%
发文量
31
审稿时长
5 weeks
期刊介绍: Food quality and safety are the main targets of investigation in food production. Therefore, reliable paths to detect, identify, quantify, characterize and monitor quality and safety issues occurring in food are of great interest. Food Quality and Safety is an open access, international, peer-reviewed journal providing a platform to highlight emerging and innovative science and technology in the agro-food field, publishing up-to-date research in the areas of food quality and safety, food nutrition and human health. It promotes food and health equity which will consequently promote public health and combat diseases. The journal is an effective channel of communication between food scientists, nutritionists, public health professionals, food producers, food marketers, policy makers, governmental and non-governmental agencies, and others concerned with the food safety, nutrition and public health dimensions. The journal accepts original research articles, review papers, technical reports, case studies, conference reports, and book reviews articles.
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