Food Quality and Safety最新文献

{"title":"Physicochemical properties, stability, and oxidation kinetics of <i>Rosa roxburghii</i> Tratt seed oil/β-carotene proliposomes modified with sodium alginate and chitosan bilayers","authors":"Yanling Lu, Huimin Bi, Yiting Feng, Jiaojiao Yang, Long Han, Lei Guo, Fangyu Fan","doi":"10.1093/fqsafe/fyaf065","DOIUrl":"https://doi.org/10.1093/fqsafe/fyaf065","url":null,"abstract":"Abstract Objectives To enhance the stability of proliposomes co-encapsulating Rosa roxburghii Tratt seed oil (RSO) and β-carotene (βC), sodium alginate (SA) and chitosan (CS) were utilized for bilayer surface modification. In this study, the proliposomes containing RSO and βC are referred to as Ps, while the modified formulations are denoted as sodium alginate–chitosan-modified proliposomes (SA-CS-Ps). Materials and methods The physicochemical properties, structural characteristics, thermal stability, microstructure, rheological behavior, storage stability, and oxidation kinetics of SA-CS-Ps were investigated and compared with those of unmodified Ps and CS monolayer-modified Ps (CS-Ps). Results The particle size and turbidity of Ps increased with the addition of modification layers, reaching 563.73 nm and 0.697, respectively, for SA-CS-Ps. CS and SA formed a protective bilayer through electrostatic interactions, which preserved the spherical structure of Ps while enhancing its apparent viscosity and stress. Thermal stability analysis indicated that the phase transition temperatures of CS-Ps and SA-CS-Ps were elevated by 10.57 and 15.76 °C, respectively, compared with that of unmodified Ps. During storage, the retention rates (RRs) of RSO and βC followed the order SA-CS-Ps&amp;gt;CS-Ps&amp;gt;Ps, whereas malondialdehyde and peroxide values exhibited the opposite trend. Elevated temperatures accelerated oxidation, thereby compromising the protective effect of the carriers. After 24 d at 4 °C, SA-CS-Ps demonstrated superior stability, with malondialdehyde of 0.016 μg/mg, peroxide value of 2.697 mmol/kg, and RRs of RSO of 97.15% and βC of 96.37%. The oxidation kinetics conformed to a first-order reaction model. Conclusions Given its enhanced protection against degradation and stability, SA-CS-Ps represents a promising candidate for the delivery of sensitive bioactive compounds. This study provides a theoretical basis for the application of bilayer-modified proliposomes in functional food delivery systems requiring enhanced stability.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147967061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat-induced formation of β-lactoglobulin–milk fat globule membrane complexes: A molecular analysis","authors":"Xiaoyue Wang, Ke Zhao, Zhongna Yu, Chuozi Liang, Hongning Jiang, Qijing Du, Rongbo Fan, Yongxin Yang, Rongwei Han","doi":"10.1093/fqsafe/fyaf058","DOIUrl":"https://doi.org/10.1093/fqsafe/fyaf058","url":null,"abstract":"Abstract The heat-induced formation of molecular polymers between β-lactoglobulin (β-LG) and the milk fat globule membrane (MFGM) has been widely recognized; nevertheless, the molecular mechanisms underlying this formation remain unclear. This study aimed to investigate the binding mechanisms and physicochemical properties of complexes formed between β-LG and MFGM under two distinct heat treatment conditions: 63 °C for 30 min and 90 °C for 15 min. Differential scanning calorimetry analysis indicated that heating the complexes at 90 °C for 15 min enhanced their thermal stability. Following this treatment, MFGM-protein complexes exhibited decreased intrinsic fluorescence intensity and a blue shift in the maximum emission wavelength. To elucidate the molecular mechanisms underlying complex formation, blocking agents including dithiobis-nitrobenzoic acid, N-ethylmaleimide, and dithioerythritol were applied at both 63 °C/30 min and 90 °C/15 min, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The results demonstrate that β-LG–MFGM complex formation involves disulfide bonds (notably Cys121), hydrogen bonding, hydrophobic interactions, and electrostatic interactions, in addition to general intermolecular forces. Heat treatment was found to promote both the formation and thermal stability of these complexes. These findings provide a mechanistic basis for further investigation of how heat processing influences the digestive behavior and functional properties of β-LG–MFGM complexes.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://academic.oup.com/fqs/advance-article-pdf/doi/10.1093/fqsafe/fyaf058/64855438/fyaf058.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147889194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-renal amyloidosis potential of rosemary extract: A multidimensional mechanistic study","authors":"Tianyou Li, Gangqiang Liu, Susan Liu, Changxin Ye, Ziyi Tang, Pei‐Hui Wang, Guirong Borijihan, Lijian Cui, Yan Zhang, Yun Huang","doi":"10.1093/fqsafe/fyaf055","DOIUrl":"https://doi.org/10.1093/fqsafe/fyaf055","url":null,"abstract":"Abstract Objectives Amyloid protein buildup in the kidneys leads to renal amyloidosis (RA), which impairs kidney function and often progresses to end-stage renal disease. The underlying mechanisms are not fully understood, and effective treatments are lacking. Rosemary (Rosmarinus officinalis L.), an edible spice and medicinal herb, has demonstrated promising renal protective effects in recent years. Nevertheless, further investigation is needed to determine its effect on RA. Materials and methods A lysozyme amyloid-like fibril model (M-LYSO) was added to MES13 cell cultures or administered intravenously to C57BL/6 mice to mimic the pathological microenvironment of amyloidosis. Spectroscopic, histological, and molecular mechanistic analyses were performed to evaluate the modulatory effects of rosemary ethanol extract (REE) on RA using both in vitro and in vivo models. Results Spectroscopic analysis revealed that REE disrupted amyloid fibril structures and reduced the aggregation propensity of amyloid proteins. In vitro and in vivo studies have shown that REE alleviated cellular damage to MES13 cells by improving subcellular function. It restored calcium homeostasis and eliminated reactive oxygen species, which inhibited the PERK/ATF-4/CHOP endoplasmic reticulum stress pathway and the apoptosis pathway. Continuous oral administration of REE at doses of 100 and 200 mg/kg for 8 weeks significantly mitigated pathological changes in renal tissues and restored kidney function in the RA model. Conclusions These findings suggest that rosemary is a promising candidate for adjuvant therapy for RA.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://academic.oup.com/fqs/advance-article-pdf/doi/10.1093/fqsafe/fyaf055/64831556/fyaf055.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147888317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening out key organic acids for inducing diarrhea in fermented blueberry wine based on the spectrum-effect relationship","authors":"Wenwen Zeng, Baoting Liu, Yulong Ding, Xiang Ao, Qiang Yu","doi":"10.1093/fqsafe/fyaf056","DOIUrl":"https://doi.org/10.1093/fqsafe/fyaf056","url":null,"abstract":"Abstract Fermented blueberry wine (BW) contains organic acids that may contribute to gastrointestinal discomfort, but the specific compounds responsible for diarrhea remain unclear. This study aimed to identify the key diarrhea-inducing organic acids by establishing a spectrum-effect relationship between chemical composition and biological activity. Ultra-high performance liquid chromatography (UHPLC) analysis revealed eight major organic acids in BW, with quinic acid predominating. Eight anion-exchange resin pretreatments combined with UHPLC-diode array detector successfully characterized the organic acid profiles of BW. Subsequent studies in mice demonstrated that resin treatment significantly alleviated BW-induced diarrhea in mice, reducing the diarrhea index and attenuating intestinal propulsion. Histopathological and biochemical analyses demonstrated restored intestinal integrity, improved gut barrier function, and normalized serum electrolytes, confirming that organic acids are the causative agents. Further spectrum-effect relationship was established through grey relational, orthogonal partial least squares, and Spearman rank correlation analysis, revealing quinic acid as exhibiting the strongest positive correlation with diarrhea incidence. The findings suggest that quinic acid is a primary contributor to diarrheal effects observed in BW. This study provides critical insights into regulating components in BW production to mitigate adverse gastrointestinal effects and a methodological foundation for material basis research in functional foods.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://academic.oup.com/fqs/advance-article-pdf/doi/10.1093/fqsafe/fyaf056/64831847/fyaf056.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Directional evolution of progesterone receptor and development of an enhanced fluorescence polarization assay for screening of progestins in milk","authors":"Jiaxuan Chang, Zuqiang Jiang 姜祖强, Jianping Wang 王建平","doi":"10.1093/fqsafe/fyaf036","DOIUrl":"https://doi.org/10.1093/fqsafe/fyaf036","url":null,"abstract":"Abstract Objective Fluorescence polarization immunoassay, a type of rapid and simple method, has been used to determine many veterinary drugs in food samples. However, no studies have reported the use of this method for the determination of progestins in food samples. In addition, the sensitivity of fluorescence polarization immunoassay is usually low, thus, a strategy capable of improving its sensitivity is desirable. Materials and Methods LEU718 in the binding cavity of the human progesterone receptor was replaced with glutamine (GLN) to produce a mutant based on virtual mutation and site-directed mutagenesis. A streptavidin-/biotin-based fluorescent tracer system was synthesized as a signal amplification system that was combined with the mutant to develop an enhanced fluorescence polarization assay for screening of progestins in milk. Results Molecule docking showed that the mutant exhibited improved recognition abilities for five progestins due to increased intermolecular forces. The method sensitivities for the five drugs were 22.6- to 68.5-fold greater (limits of detection of 0.14–0.6 ng/mL) in comparison with those of the conventional fluorescein-based signal system. As a result, the milk sample only needed a tenfold dilution prior to analysis. In addition, the method sensitivities for the five drugs when using the mutant increased 2.2- to 5.2-fold in comparison with those of the parental receptor, confirming successful evolution. Conclusions This enhanced fluorescence polarization assay can be used as a practical tool for the routine screening of progestins in milk. Under the guidance of the present study, similar single-site/multisite evolution techniques for improving the recognition ability of a receptor or antibody may be reported in the future, and more signal amplification systems based on novel fluorophores may be created in the future.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://academic.oup.com/fqs/advance-article-pdf/doi/10.1093/fqsafe/fyaf036/64146399/fyaf036.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of high-precision quantitative analysis detection for three yeasts based on CRISPR/Cas12a","authors":"Jinyu Hu, Xiaogang Liu, Tiantian Liu, Ting Zhao, Mingliang Li, Qilin Yang, Xiaofeng Jiang, Yanfei Xiong, Caihong Shen, Shuangping Liu, Jian Mao, Ruikang Tu","doi":"10.1093/fqsafe/fyaf017","DOIUrl":"https://doi.org/10.1093/fqsafe/fyaf017","url":null,"abstract":"Abstract Objectives Yeast plays a crucial role in fermented foods, yet the application of the CRISPR/Cas system for yeast detection has been rarely reported. The objective of this study is to develop a rapid and quantitative detection method for brewing yeast based on the CRISPR-Cas12a system, aiming to achieve higher sensitivity than quantitative real-time polymerase chain reaction (qPCR) assay and fill the gap in the application of CRISPR for yeast detection in fermented foods. Materials and Methods This work established one-step Recombinase Polymerase Amplification (RPA)-CRISPR/Cas12a (ORCC) detection for quantifying yeasts in fermented foods by integrating target amplification and the CRISPR system into a one-pot reaction. Results A numerical standardized definition was established for the quantifiable state in ORCC detection of yeasts. Using ORCC detection, which occurs at 37 °C, absolute quantification of Saccharomyces cerevisiae, Wickerhamomyces anomalus, and Zygosaccharomyces bailii can be completed in less than 45 min. Under optimal conditions, the limits of quantification and the limits of detection for three yeasts were 100 copies/μL and 1 copy/μL, with no cross-reaction observed among non-target yeasts. In the pure yeast samples, ORCC detection of the target yeasts achieved a detection rate of 91.60%–116.57%. In the simulated fermentation samples, the recovery rate for spiked target yeasts using ORCC detection ranged from 92.78% to 106.90%. In the original naturally fermented samples, target yeasts can be quantified by ORCC detection but not by qPCR assay. In the spiked naturally fermented samples, the recovery rates of the qPCR assay were generally lower than those of ORCC detection. Conclusions ORCC detection is simple, rapid, accurate, and highly sensitive for detecting three yeasts in fermented foods. It has significant potential for precisely monitoring yeast growth and fermentation process in various fermentation systems.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://academic.oup.com/fqs/advance-article-pdf/doi/10.1093/fqsafe/fyaf017/63372006/fyaf017.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147885770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autofluorescence-free optical strategy for detection of H2O2 based on persistent luminescence probes","authors":"Beibei Wang, Ling Sun, Shugang Li, Kai Liu, Min Yang, Di Wu, Jinqiu Wang, Fang Geng","doi":"10.1093/fqsafe/fyaf040","DOIUrl":"https://doi.org/10.1093/fqsafe/fyaf040","url":null,"abstract":"Abstract Objectives: Hydrogen peroxide (H2O2) not only is widely used in industrial processes but also participates in many biological reactions. However, excessive levels cause great harm to the human body. Here, we designed a sensitive and autofluorescence-free persistent luminescence (PL) method for H2O2 detection based on persistent luminescence nanoparticles (PLNPs) and a specific chemical reaction. Materials and Methods: A detection probe, PLNPs@MnO2, was constructed by coating the surface of PLNPs with a manganese dioxide (MnO2) shell. The quenched PL of the PLNPs@MnO2 could be turned on by H2O2, and the restored PL was positively correlated with H2O2 in a certain concentration range. Results: The developed method was linear in the range of 0–5 μmol/L with a detection limit of 0.079 μmol/L in solution and a linear response range of 0–1000 μmol/L for naked-eye detection on flat plates. Therefore, the detection system is suitable not only for the traditional solution state but also for naked-eye detection on a flat plate, allowing for rapid inspection on-site in resource-limited areas. The applicability of the developed method was demonstrated by analyzing bottled purified water, milk, and contact lens solution samples for H2O2 with the recoveries of 90.56%–109.73%.Conclusion: This study demonstrates a versatile and reliable persistent luminescence-based strategy for sensitive and visual detection of H2O2, showing great potential for applications in environmental and biological sample analysis.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://academic.oup.com/fqs/advance-article-pdf/doi/10.1093/fqsafe/fyaf040/64159392/fyaf040.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147895706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Octacosanol preserves postharvest apple quality by delaying senescence through coordinated regulation of cuticular wax biosynthesis and antioxidant defense mechanisms","authors":"Mengmeng Wei, Quan Sun, A-ning Wang, Lian‐Da Du, Wenyan Wang, Min Qiao, Zhongnan Nie, Ta-Jen Chu, Ze Chen, Xue Bai, Da‐Gang Hu","doi":"10.1093/fqsafe/fyaf015","DOIUrl":"https://doi.org/10.1093/fqsafe/fyaf015","url":null,"abstract":"Abstract Apples, as respiratory climacteric fruit, undergo postharvest ripening and senescence, impacting commodity value. Cuticular wax protects against environmental stresses. Here, gas chromatography-mass spectrometry (GC-MS) analysis revealed a decline in fatty alcohol levels in apple wax during storage, correlating with fruit quality deterioration. Notably, octacosanol content significantly decreased with storage, suggesting that it is a primary cause of wax and fruit quality decline. Octacosanol treatment improved fruit quality and delayed softening by enhancing wax synthesis and antioxidant levels and suppressing cell wall-degrading enzymes. Transcriptome sequencing and real-time quantitative polymerase chain reaction (RT-qPCR) assays indicated increased expression of wax, peroxidase, sucrose, and starch genes and decreased expression of cell wall degradation genes, explaining octacosanol’s benefits. This study provides a theoretical basis for octacosanol application in fruit preservation.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://academic.oup.com/fqs/advance-article-pdf/doi/10.1093/fqsafe/fyaf015/62914747/fyaf015.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-based identification and purification of bacteriocin Q13 against methicillin-resistant <i>Staphylococcus aureus</i> from <i>Lactococcus lactis</i> Q13","authors":"Yangyang Yang, Shuang Liu, Qian Wan, Yuan Liu, Likou Zou, Daoying Wang, Chenglin Zhu, Junni Tang","doi":"10.1093/fqsafe/fyaf044","DOIUrl":"https://doi.org/10.1093/fqsafe/fyaf044","url":null,"abstract":"Abstract Objectives Staphylococcus aureus (MRSA) is widely acknowledged as a significant threat to food safety and human health. This research focused on the characteristics of bacteriocin Q13 produced by Lactococcus lactis Q13 isolated from the traditional Chinese fermented food hairy tofu. Materials and Methods The biosynthetic gene clusters of bacteriocins were identified through whole-genome sequencing of L. lactis Q13. The antibacterial activity of its cell-free supernatant was assessed against MRSA and other Gram-positive bacteria. Subsequently, bacteriocin Q13 was purified through ammonium sulfate precipitation, Sephadex G-50 gel filtration chromatography, and reversed-phase high-performance liquid chromatography. Its molecular weight was determined via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and structural features were analyzed by Fourier-transform infrared spectroscopy. Stability of bacteriocin Q13 under various conditions (enzymes, organic solvents, surfactants, metal ions, UV exposure, NaCl concentration, and storage time and temperature) was evaluated. The antimicrobial mode of action was investigated via scanning electron microscopy (SEM). Results Bacteriocin Q13 exhibited a significant bacteriostatic effect against MRSA and a broad spectrum of Gram-positive bacteria. Its molecular weight was 19367.97 Da, with dominant secondary structures identified as β-turn and β-sheet. Bacteriocin Q13 demonstrated remarkable stability under different conditions. SEM analysis showed that bacteriocin Q13 was able to induce significant morphological changes in MRSA, including disruption of cellular morphology, impairment of membrane integrity, and leakage of cytoplasmic contents, revealing the bacteriostatic mechanism of bacteriocin Q13 against MRSA. Conclusions Bacteriocin Q13 from L. lactis Q13 exhibits considerable potential as a natural antimicrobial compound for controlling MRSA contamination in food products. These results lay a scientific foundation for the application of L. lactis Q13 and its bacteriocin in the food industry as potential probiotics and preservatives.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://academic.oup.com/fqs/advance-article-pdf/doi/10.1093/fqsafe/fyaf044/64219623/fyaf044.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of citronellol in sweetpotato black spot disease using a quartz crystal microbalance (QCM) gas sensor based on Co/Zn-ZIF@MIP composite","authors":"Xinghua Lu, Suqi Liu, Lu Zhang, Chenglong Wang, Zhenhe Wang, Linjiang Pang, Guoquan Lu, Jiyu Cheng, Mingyi Yang, Le Gao, Wei Chen, Yuge Guan","doi":"10.1093/fqsafe/fyaf029","DOIUrl":"https://doi.org/10.1093/fqsafe/fyaf029","url":null,"abstract":"Abstract Citronellol is a kind of terpene produced by plants in response to external stress; thus can be used as a gas biomarker to detect black spot Ceratocystis fimbriata infection in sweetpotato. However, many contemporary analytical methods, exemplified by gas chromatography-mass spectrometry, are technically demanding, time-consuming, and require complex sample preparation procedures. In this study, a quartz crystal microbalance (QCM)-based gas sensor fabricated via a surface molecular imprinting technique was modified with a Co/Zn-ZIF@MIP composite, in which cobalt–zinc bimetallic ZIF (Co/Zn-ZIF) served as the support material. A linear relationship was observed between the frequency shift and citronellol concentrations ranging from 0.88 to 22 mg/L, with a sensitivity of −6.08 Hz/(mg·L) and a limit of detection (LOD) of 1.35 mg/L. This result indicated that this sensor has excellent selectivity for citronellol and demonstrates high repeatability, as evidenced by R² value of 0.97. In evaluations with real samples, the sensor reliably identified citronellol among the complex volatile organic compounds (VOCs) emitted from black spot-infected sweetpotato, indicating a high level of selectivity. Our research achieved the rapid characterization of sweetpotato black spot disease within 4 min and provided new insights into the development of QCM-based gas sensors for the rapid assessment of agricultural product quality and safety.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://academic.oup.com/fqs/advance-article-pdf/doi/10.1093/fqsafe/fyaf029/63593511/fyaf029.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}