Frontiers in Chemistry最新文献

The detection of serum-volatile organic compounds in the diagnostics of hepatocellular carcinoma using gas chromatography-ion mobility spectrometry. 气相色谱-离子迁移谱法检测肝细胞癌诊断中的血清挥发性有机物。

IF 4.2 3区化学

Frontiers in Chemistry Pub Date : 2025-10-07 eCollection Date: 2025-01-01 DOI: 10.3389/fchem.2025.1672220

Xin Shen, Chenglin Xie, Shijia Zhang, Wen Ai, Ziran Yu, Xu Ye

{"title":"The detection of serum-volatile organic compounds in the diagnostics of hepatocellular carcinoma using gas chromatography-ion mobility spectrometry.","authors":"Xin Shen, Chenglin Xie, Shijia Zhang, Wen Ai, Ziran Yu, Xu Ye","doi":"10.3389/fchem.2025.1672220","DOIUrl":"https://doi.org/10.3389/fchem.2025.1672220","url":null,"abstract":"Introduction: The third most common cause of cancer-related death worldwide is hepatocellular carcinoma (HCC), a malignant liver tumor that usually arises in patients with cirrhosis and chronic liver disease. New biomarkers are required for bidirectional validation of HCC in clinical practice because of its early asymptomatic stage, high mortality rate, and rapid tumor growth, as well as the high rate of false-positive results obtained for the commonly used serum marker alpha-fetoprotein (AFP).Methods: This study used gas chromatography-ion mobility spectrometry (GC-IMS) combined with chemometrics to analyze the differences in volatile organic compounds (VOCs) in the serum of patients with and without hepatocellular carcinoma (HCC) in order to explore new biomarkers of HCC.Results: The GC-IMS analysis detected 31 VOCs in serum, including 8 ketones, 11 aldehydes, 6 alcohols, 2 esters, and 1 furans, many of which differed in content between the controls and the patients with HCC.Conclusions: The VOCs obtained from the use of these analytical devices can serve as a reference for developing low-cost equipment in the future. Considering its high efficiency and low cost, the detection of volatile organic compounds can be used as an auxiliary means of diagnosis or for mass screening of the population.","PeriodicalId":12421,"journal":{"name":"Frontiers in Chemistry","volume":"13 ","pages":"1672220"},"PeriodicalIF":4.2,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12537714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145344501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational insights into novel inhibitors: virtual screening of small molecules against human carbonic anhydrase II. 计算见解到新的抑制剂：虚拟筛选小分子对抗人类碳酸酐酶II。

IF 4.2 3区化学

Frontiers in Chemistry Pub Date : 2025-10-02 eCollection Date: 2025-01-01 DOI: 10.3389/fchem.2025.1627793

Sermarajan Arunachalam, Balamurali M M, Ramachandran Gnanasekaran

{"title":"Computational insights into novel inhibitors: virtual screening of small molecules against human carbonic anhydrase II.","authors":"Sermarajan Arunachalam, Balamurali M M, Ramachandran Gnanasekaran","doi":"10.3389/fchem.2025.1627793","DOIUrl":"10.3389/fchem.2025.1627793","url":null,"abstract":"Carbonic anhydrases, zinc-based metalloproteins, facilitate the reversible conversion of CO2 into carbonic acid when transported through blood vessels and subsequently regulate the physiological pH. In humans, this enzyme has been the therapeutic target for numerous diseases, as its abnormal regulation leads to a variety of disorders. The regulatory mechanism of this enzyme includes targeting catalytic Zn2+ ions as well as the residues that significantly regulate the protein's structure and stability. With the available data on numerous sulfonamides, sulfamates, sulfamides, and non-sulfamide-derived inhibitors, in this study, a library of sulfonamide, extended aromatic sulfonamide, and non-sulfonamide derivatives was screened using a fragment-based drug discovery approach. Virtual screening was performed with molecular docking (DOCK 6 and Schrödinger GLIDE), rescored using MM-GBSA, and validated over 100-ns molecular dynamics simulations. Pharmacophore models were developed to identify key interaction features, while pharmacokinetic profiles were evaluated to assess their drug-likeness. Compounds S8 (sulfonamide) and S15-S16 (non-sulfonamides) emerged as promising inhibitors, showing strong Zn2+ coordination and stable binding to residues His93, Leu196, Thr197, and Thr198 that favor pharmacokinetic properties. The results provide atomistic insights into carbonic anhydrase II (CAII) inhibition and identify potential leads for further experimental validation.","PeriodicalId":12421,"journal":{"name":"Frontiers in Chemistry","volume":"13 ","pages":"1627793"},"PeriodicalIF":4.2,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12528100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145328581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting RNA-binding proteins with small molecules: perspectives and challenges. 靶向小分子rna结合蛋白：观点和挑战。

IF 4.2 3区化学

Frontiers in Chemistry Pub Date : 2025-10-02 eCollection Date: 2025-01-01 DOI: 10.3389/fchem.2025.1649692

Olivier Mpungi Konde, Williams Balela Balela, Tania Bishola Tshitenge

引用次数: 0

Comprehensive medicinal chemistry survey highlights a portfolio of lead molecules for Alzheimer's disease therapy. 综合药物化学调查强调了阿尔茨海默病治疗的铅分子组合。

IF 4.2 3区化学

Frontiers in Chemistry Pub Date : 2025-10-01 eCollection Date: 2025-01-01 DOI: 10.3389/fchem.2025.1642190

Pravin R Bhansali, Shashank M Sonkusare, Shubhangi S Savale, Yasanandana S Wijayasinghe, Yini Liao, Douglas C Sloan, Ganesh U Chaturbhuj, Brian S Muntean

{"title":"Comprehensive medicinal chemistry survey highlights a portfolio of lead molecules for Alzheimer's disease therapy.","authors":"Pravin R Bhansali, Shashank M Sonkusare, Shubhangi S Savale, Yasanandana S Wijayasinghe, Yini Liao, Douglas C Sloan, Ganesh U Chaturbhuj, Brian S Muntean","doi":"10.3389/fchem.2025.1642190","DOIUrl":"10.3389/fchem.2025.1642190","url":null,"abstract":"The World Health Organization reports 10 million new patients with dementia each year. The most common form of dementia is Alzheimer's disease (AD), which constitutes up to 70% of cases. AD is mainly characterized by loss of memory, which, in addition to its debilitating individual effect, represents a burden of 1.3 trillion US dollars globally. The staggering scale of hardship has spurred intense investigations from the scientific community in search of therapeutic solutions. Recent advances to combat AD involve the identification of numerous neural targets and concomitant chemical interventions as nodes of therapy. Due to disparate biological and chemical facets of AD therapy, a comprehensive perspective covering both arenas is currently missing from the literature. This perspective aims to provide an extensive understanding of anti-AD mechanics alongside small-molecule drug design efforts from a medicinal chemist viewpoint. We are confident that this survey of the literature will provide a resourceful motivation to propel future research efforts towards successful Alzheimer's disease therapy.","PeriodicalId":12421,"journal":{"name":"Frontiers in Chemistry","volume":"13 ","pages":"1642190"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress in the application of hydrophilic interaction liquid chromatography for the separation and analysis of traditional Chinese medicine. 亲水相互作用液相色谱在中药分离分析中的应用进展。

IF 4.2 3区化学

Frontiers in Chemistry Pub Date : 2025-10-01 eCollection Date: 2025-01-01 DOI: 10.3389/fchem.2025.1694998

Yuanyuan Wu, Hui Huang, Xianglong Zhao, Junhao Lin, Jialiang Guo, Zhengjin Jiang, Kesara Ar-Sanork, Tao Deng, Dongsheng Xu

{"title":"Recent progress in the application of hydrophilic interaction liquid chromatography for the separation and analysis of traditional Chinese medicine.","authors":"Yuanyuan Wu, Hui Huang, Xianglong Zhao, Junhao Lin, Jialiang Guo, Zhengjin Jiang, Kesara Ar-Sanork, Tao Deng, Dongsheng Xu","doi":"10.3389/fchem.2025.1694998","DOIUrl":"10.3389/fchem.2025.1694998","url":null,"abstract":"Traditional Chinese medicine (TCM), with a history spanning over 2,000 years, contains numerous strongly polar components that are increasingly the focus of pharmaceutical and analytical research. However, conventional reversed-phase liquid chromatography usually fails to adequately retain and separate these highly polar components. Hydrophilic interaction liquid chromatography (HILIC) has emerged as a powerful complementary technique, offering improved retention and separation for polar analytes. In recent years, HILIC has seen rapid development and wide application in the analysis of TCM. This review summarizes the major types of HILIC stationary phases and their recent applications in the separation of polar components in TCM. Particular attention is given to the analysis, quality control, and metabolomic profiling of bioactive compounds such as flavonoids, alkaloids, and saponins, highlighting the increasingly important role of HILIC in promoting TCM research.","PeriodicalId":12421,"journal":{"name":"Frontiers in Chemistry","volume":"13 ","pages":"1694998"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding honey-sweet flavored flue-cured tobacco from Guizhou with data science and flavoromics by volatile and cell wall components. 利用挥发性成分和细胞壁成分的数据科学和风味组学对贵州蜜甜烤烟进行解码。

IF 4.2 3区化学

Frontiers in Chemistry Pub Date : 2025-10-01 eCollection Date: 2025-01-01 DOI: 10.3389/fchem.2025.1613828

Risheng Zhong, Zhenchun Sun, Liang Feng, Haitao Chen, Shuqi Wang, Yechun Lin, Jie Sun, Ning Zhang, Huiying Zhang, Feng Wang

{"title":"Decoding honey-sweet flavored flue-cured tobacco from Guizhou with data science and flavoromics by volatile and cell wall components.","authors":"Risheng Zhong, Zhenchun Sun, Liang Feng, Haitao Chen, Shuqi Wang, Yechun Lin, Jie Sun, Ning Zhang, Huiying Zhang, Feng Wang","doi":"10.3389/fchem.2025.1613828","DOIUrl":"10.3389/fchem.2025.1613828","url":null,"abstract":"Nicotiana tabacum L. is often called tobacco. The aroma of flue-cured tobacco (FCT) varies according to the origin and grade. In this study, volatiles and plant cell wall components (CWC) were used to differentiate aroma types and grades of FCT, with a focus on the honey-sweet flavored FCT from Guizhou, China. Volatiles were analyzed by headspace solid-phase microextraction gas chromatography/mass spectrometry, while CWC (cellulose, hemicellulose, pectin, lignin) were quantified. Results indicated that upper leaf (Grade B) tobacco contained higher volatile levels than middle leaf (Grade C). Multivariate analyses-Principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), and logistic regression (LR) identified 27 key volatiles contributing to aroma differentiation in FCT origin. By combining the screened volatiles with odor activity value, the most important key aroma compounds that distinguish Guizhou honey-sweet flavored from other origins were β-cyclocitral and 1-nonanal. The CWC showed significant variation across origins or grades. Machine learning models (e.g., LR with 96.5% accuracy) effectively distinguished the origin of FCT. This study pioneers the integration of machine learning with molecular sensory science to decode the unique honey-sweet flavor of Guizhou flue-cured tobacco, addressing a critical gap in linking volatile biomarkers to regional terroir. This methodology provides a way to evaluate tobacco quality and aroma characteristics.","PeriodicalId":12421,"journal":{"name":"Frontiers in Chemistry","volume":"13 ","pages":"1613828"},"PeriodicalIF":4.2,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12521107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145307516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a dichlorosalicylaldehyde-derived fluorescent probe for monitoring glutathione in a cellular pulmonary ventilation model. 二氯水杨醛衍生的荧光探针用于监测细胞肺通气模型中的谷胱甘肽。

IF 4.2 3区化学

Frontiers in Chemistry Pub Date : 2025-09-30 eCollection Date: 2025-01-01 DOI: 10.3389/fchem.2025.1698116

Xiao Zhou, Lei Zhang, Yuwen Lao, Bin Zhou, Zhongquan Zhu

引用次数: 0

Facile construction of Pt/TiO₂/Se/Ni heterostructure for efficient visible-light-driven PEC water splitting. Pt/TiO2/Se/Ni异质结构的快速构建，用于高效的可见光驱动PEC水分解。

IF 4.2 3区化学

Frontiers in Chemistry Pub Date : 2025-09-29 eCollection Date: 2025-01-01 DOI: 10.3389/fchem.2025.1688359

Ying-Chu Chen, Yen-Wei Huang, Yu-Kuei Hsu

{"title":"Facile construction of Pt/TiO2/Se/Ni heterostructure for efficient visible-light-driven PEC water splitting.","authors":"Ying-Chu Chen, Yen-Wei Huang, Yu-Kuei Hsu","doi":"10.3389/fchem.2025.1688359","DOIUrl":"10.3389/fchem.2025.1688359","url":null,"abstract":"In this study, a novel Pt/TiO2/Se/Ni heterostructure photocathode was successfully fabricated via a simple and cost-effective method involving galvanic replacement, thermal annealing, and sequential spin-coating processes. Amorphous selenium was first deposited on nickel foil and subsequently transformed into crystalline trigonal Se through thermal treatment. The TiO2 and Pt nanoparticles were then uniformly decorated onto the Se surface to form a hierarchical heterostructure. Structural, morphological, and compositional characterizations using XRD, SEM, Raman spectroscopy, and XPS confirmed the formation of trigonal selenium and the successful deposition of TiO2 and Pt. Optical and photoelectrochemical (PEC) analyses revealed that the crystalline Se exhibited an optimal band gap of 1.89 eV and efficient visible light absorption. The Pt/TiO2/Se photocathode delivered a significantly enhanced photocurrent density of -5 mA cm-2 at -0.3 V vs. Ag/AgCl, which is 1.6 times higher than that of the bare Se electrode. Mott-Schottky and EIS analyses demonstrated an increased carrier density and reduced charge transfer resistance, facilitating efficient charge separation and transfer. These findings highlight the great potential of the Pt/TiO2/Se heterostructure as a high-performance photocathode for solar hydrogen production applications.","PeriodicalId":12421,"journal":{"name":"Frontiers in Chemistry","volume":"13 ","pages":"1688359"},"PeriodicalIF":4.2,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12515969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145291694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hardtank^® technology in extraction of caffeine and chlorogenic acid from coffee: analysis of chemical composition and antioxidant properties of coffee beverages. 从咖啡中提取咖啡因和绿原酸的Hardtank®技术：分析咖啡饮料的化学成分和抗氧化性能。

IF 4.2 3区化学

Frontiers in Chemistry Pub Date : 2025-09-26 eCollection Date: 2025-01-01 DOI: 10.3389/fchem.2025.1681137

Radosław Kowalski, Kamil Belniak, Grażyna Kowalska, Marzena Włodarczyk-Stasiak, Artur Mazurek, Tomasz Baj

{"title":"Hardtank® technology in extraction of caffeine and chlorogenic acid from coffee: analysis of chemical composition and antioxidant properties of coffee beverages.","authors":"Radosław Kowalski, Kamil Belniak, Grażyna Kowalska, Marzena Włodarczyk-Stasiak, Artur Mazurek, Tomasz Baj","doi":"10.3389/fchem.2025.1681137","DOIUrl":"10.3389/fchem.2025.1681137","url":null,"abstract":"Introduction: Hardtank® is a pressure-modulated, solvent-free cold extraction that shortens brewing while maximizing bioactive recovery from roasted coffee.Methods: We produced a Nitro Cold Brew beverage from roasted Yellow Bourbon beans using Hardtank® (550 g : 10,000 mL water, 30 min, 0.3-1.5 bar) and compared its composition and antioxidant activity with laboratory extracts from roasted and green beans obtained by hot water infusion (93 °C, 5 min) and by methanol extraction (70% reflux, 2 h; 100% reflux, 2 h; 70% shake, 5 min). We measured caffeine, chlorogenic acid (and derivatives), total phenolics, total flavonoids, and antioxidant activity by the ferric reducing antioxidant power and DPPH radical tests, expressed as Trolox equivalents.Results and discussion: Nitro Cold Brew contained 375.8±2.4 mg phenolics + flavonoids per 100 mL, 72.4±1.1 mg caffeine per 100 mL, and 78.2±2.3 mg chlorogenic acid per 100 mL, with ferric reducing antioxidant power 27.9±0.5 mM and DPPH 11.7±0.6 mM. Using 70% methanol reflux as the 100% reference, Hardtank® achieved 135.3% for phenolics + flavonoids, 119.7% for caffeine, and up to 172.3% for chlorogenic acid. Overall, Hardtank® yielded a ready-to-drink coffee with bioactive and antioxidant profiles comparable to or better than laboratory reflux extracts, while operating rapidly and without organic solvents, indicating promise for scalable production of functional coffee beverages.","PeriodicalId":12421,"journal":{"name":"Frontiers in Chemistry","volume":"13 ","pages":"1681137"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12511873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the structure-performance relationship between binder types and aluminum-based lithium adsorbent. 粘结剂类型与铝基锂吸附剂结构性能关系的研究。

IF 4.2 3区化学

Frontiers in Chemistry Pub Date : 2025-09-26 eCollection Date: 2025-01-01 DOI: 10.3389/fchem.2025.1628941

Ben Ma, Xiaoyu Wang, Jing Zhou, Lijuan Zhang, Ruibin Liu, Li Su, Wenlong Wang, Qinglei Wang, Ping Li, Xuehui Shangguan, Faqiang Li

{"title":"Study on the structure-performance relationship between binder types and aluminum-based lithium adsorbent.","authors":"Ben Ma, Xiaoyu Wang, Jing Zhou, Lijuan Zhang, Ruibin Liu, Li Su, Wenlong Wang, Qinglei Wang, Ping Li, Xuehui Shangguan, Faqiang Li","doi":"10.3389/fchem.2025.1628941","DOIUrl":"10.3389/fchem.2025.1628941","url":null,"abstract":"Aluminum lithium layered double hydroxides adsorbents (Li/Al-LDH) are used in industry due to their mild adsorption/desorption conditions, good stability and low cost. However, traditional powdered aluminum lithium adsorbents exhibit poor fluidity and a relatively high dissolution rate. The granulation strategy using binders is employed to address the aforementioned challenges. Nevertheless, there is a lack of systematic research on the relationship between the type of binder and the adsorption and desorption efficiency, as well as the kinetics and thermodynamic mechanisms of mass transfer. This work focuses on the structure-activity relationship between adsorbents and three binders (polyvinyl chloride (PVC), polyvinylidene fluoride (PVDF) and calcium alginate (SA)). The experimental results demonstrate that the adsorption/desorption performance of the adsorbent varied significantly with temperature depending on binder type. It is noted that the structures of PVDF-LDH and PVC-LDH changed during temperature changes, resulting in decreased adsorption and desorption performance. While the SA-LDH can maintain good structural stability and adsorption and desorption capabilities. Besides, in 300 ppm LiCl solution, SA-LDH presents a high adsorption and desorption capacity, with the maximum desorption capacity at 40 °C being 5.84 mg/g and the maximum adsorption capacity at 60 °C being 5.67 mg/g. This study elucidates the regulatory mechanisms of temperature on adsorption/desorption behaviors in binder-formulated granulated adsorbents, providing critical insights for optimizing industrial aluminum-based lithium adsorbents in salt lake lithium extraction.","PeriodicalId":12421,"journal":{"name":"Frontiers in Chemistry","volume":"13 ","pages":"1628941"},"PeriodicalIF":4.2,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12511872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145279322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0