Folia histochemica et cytobiologica最新文献

{"title":"Transcriptional activation and regulation of urokinase plasminogen activator inducted by LPS through MyD88 independent pathway in rat Sertoli cells.","authors":"Yan Liu,&nbsp;Kai Zhao,&nbsp;Zhe Xiong,&nbsp;Yuanxiao Yi,&nbsp;Chengliang Xiong,&nbsp;Donghui Huang,&nbsp;Hu Zhao","doi":"10.5603/FHC.a2021.0026","DOIUrl":"https://doi.org/10.5603/FHC.a2021.0026","url":null,"abstract":"<p><strong>Introduction: </strong>Urokinase plasminogen activator (uPA) is a serine protease and it also demonstrates proinflammatory properties. We thus hypothesized that uPA is involved in immunity of Sertoli cells in the rat testis.</p><p><strong>Materials and methods: </strong>The uPA gene(PLAU) promoter was cloned by RT-PCR and the transcriptional activation of core domain was screened by using Dual-Luciferase Reporter Assay System. The Sertoli cells were harvested from 20-day-old Sprague-Dawley male rats and total RNA was isolated. The uPA mRNA levels and MyD88 pathway were tested by qPCR.</p><p><strong>Results: </strong>We successfully cloned the 1517-bp rat uPA gene and screened the core domain (-455/+40) in five different fragments of uPA promoter. The uPA expression and uPA promoter activity were upregulated in lipopolysaccharide (LPS)-stimulated Sertoli cells. Furthermore, the uPA expression was regulated through the MyD88-independent pathway by interdicting IRF3 and interferon b.</p><p><strong>Conclusion: </strong>uPA expression is likely induced by LPS through the core promoter domain of uPA. This finding implied that uPA played a role in the immune function of Sertoli cells in rat testis, which might provide the development of new treatments for male infertility.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"59 4","pages":"236-244"},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39683221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroanatomical distribution of the enkephalinergic and tachykininergic systems in the alpaca brainstem: an immunohistochemical study.","authors":"Pablo Sánchez,&nbsp;Manuel Lisardo Sánchez,&nbsp;Arturo Mangas,&nbsp;Eliana de Souza,&nbsp;Luis Angel Aguilar,&nbsp;Rafael Coveñas","doi":"10.5603/FHC.a2021.0016","DOIUrl":"https://doi.org/10.5603/FHC.a2021.0016","url":null,"abstract":"<p><strong>Introduction: </strong>A recent study has shown a close neuroanatomical relationship between the enkephalinergic (methionine-enkephalin) and tachykininergic (substance P) systems in the alpaca diencephalon. In this study, our aim is to show this relationship in the alpaca brainstem.</p><p><strong>Material and methods: </strong>Using an immunohistochemical technique, the distribution of immunoreactive (Ir) fibers and cell bodies containing substance P (SP) or methionine-enkephalin (MET) has been studied in the alpaca brainstem. Five adult males were used; brain tissue was fixed and processed by standard methods.</p><p><strong>Results: </strong>SP- and MET-Ir fibers showed a widespread and similar distribution in the mesencephalon, pons and medulla oblongata. The co-localization of fibers containing SP or MET was found in most of the nuclei/tracts of the alpaca brainstem. This close neuroanatomical relationship suggests multiple physiological interactions between both neuropeptides. The distribution of the cell bodies containing SP was very restricted (cell bodies were only observed in a few nuclei located in the mesencephalon and medulla oblongata), whereas MET-Ir perikarya showed a moderately widespread distribution in the mesencephalon, pons and medulla oblongata.</p><p><strong>Conclusions: </strong>This study increases the knowledge on the neuroanatomical distribution/relationship of the tachykininergic (SP) and enkephalinergic (MET) systems in the alpaca central nervous system.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"59 3","pages":"145-156"},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39224173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and neurochemical characteristic of the cardiac nerve structures in the heart of chinchilla (Chinchilla laniger Molina).","authors":"Malgorzata Radzimirska,&nbsp;Jacek Kuchinka,&nbsp;Tadeusz Kuder,&nbsp;Elzbieta Nowak,&nbsp;Wojciech Trybus,&nbsp;Grzegorz Wrobel,&nbsp;Aleksander Szczurkowski","doi":"10.5603/FHC.a2021.0021","DOIUrl":"https://doi.org/10.5603/FHC.a2021.0021","url":null,"abstract":"<p><strong>Introduction: </strong>The heart innervation is made up of plexo-ganglionic formation containing sympathetic, parasympathetic, and sensory components. We examined the distribution and neurochemical coding of the ganglia and nerve fibers in the chinchilla's heart.</p><p><strong>Material and methods: </strong>The heart sections of 10 male and 10 female adult chinchillas were processed in accordance with the thiocholine method for acetylcholine esterase (AChE), and the SPG method for detecting the presence of adrenergic fibers was applied. The routine technique of immunohistochemical (IHC) staining with primary antibodies directed against ChAT, VAChT, DbH, TH, CART, NPY, VIP, GAL and SOM was used. The secondary antibodies were conjugated with Alexa Fluor 488 and Alexa Fluor 555 fluorophores.</p><p><strong>Results: </strong>The epicardium contained ganglia and nerve fibers, the myocardium had a few ganglion neurocytes and nerve fibers, and the endocardium contained only nerve fibers. In the epicardium, AChE-positive fibers were more prevalent than SPG-positive fibers. All the ganglion cells were immunopositive for ChAT and VAChT. Some cells also had a positive reaction to DbH and TH. Fibers containing cholinergic and adrenergic markers were numerous, while many of them were ChAT/DbH- and VAChT/TH-positive. CART/NPY and CART/VIP, as well as CART and GAL, were observed to be colocalized in ganglion neurocytes, as well as in individual cells. The nerve fibers were found to contain all the neurotransmitters we tested for, as well as the following co-occurrences: ChAT/DbH, VAChT/TH, CART/NPY, CART/VIP, CART/GAL, and CART/SOM.</p><p><strong>Conclusions: </strong>Our analysis of the neurochemical profile of the nerve structures in chinchilla's heart showed that, despite interspecies differences, the general pattern of the distribution of autonomic nervous system structures is similar to that of other mammals' species, including humans.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"59 3","pages":"157-166"},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39464553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into the pathogenesis of cardiac papillary fibroelastomas.","authors":"Natalia Matysiak,&nbsp;Lukasz Mielanczyk,&nbsp;Krzysztof Kaczmarek,&nbsp;Malgorzata Zaba,&nbsp;Edyta Reichman-Warmusz,&nbsp;Romuald Wojnicz","doi":"10.5603/FHC.a2021.0027","DOIUrl":"https://doi.org/10.5603/FHC.a2021.0027","url":null,"abstract":"<p><strong>Introduction: </strong>Cardiac papillary fibroelastomas (CPFs) are rare benign cardiac tumors typically found on the heart valves. The previously published data on the CPF focused on its clinical presentation, optimal management, and prognosis. However, histogenesis of these lesions remains controversial. Accordingly, the aim of this study was to establish the role of endocardial endothelium (EE) in CPF formation.</p><p><strong>Materials and methods: </strong>Four CPF tumors removed from the right atrioventricular valves were analyzed using hematoxylin & eosin, orcein, and Masson trichrome staining together with immunochemistry for CD-34, CD-68, vimentin, vWF and a-SMA. Moreover, conventional transmission electron microscopy was used for morphological analysis and a-SMA presence confirmation.</p><p><strong>Results: </strong>Ultrastructural morphology, immunohisto- and immunocytochemical analyses indicated that cells covering collagenous core have an endothelial origin. Some endocardial endothelium cells have the potential to undergo a transition to mesenchymal cells. Moreover, the abundant presence of extracellular vesicles may indicate an active intercellular communication. Within the intermediate translucent zone, amorphous substances with monocytes/macrophage-like cells and fibroblastic cells were found. Finally, within collagenous core activated (myo)fibroblasts were observed.</p><p><strong>Conclusions: </strong>Our study demonstrated that the endocardial endothelium of the CPF was \"double-sided\", i.e., it presented both endothelial and mesenchymal cell characteristics. Another finding was the presence of monocytes, and macrophages which were integrated into CPF core and displayed features of a fibroblast that have been shown to contribute to extracellular matrix production. This could be interpreted as being attributed to the CPF histogenesis.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"59 4","pages":"212-225"},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39956105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Golgi a-mannosidase II mediates the formation of vascular smooth muscle foam cells under inflammatory stress.","authors":"Kelan Zha,&nbsp;Qiang Ye","doi":"10.5603/FHC.a2021.0015","DOIUrl":"https://doi.org/10.5603/FHC.a2021.0015","url":null,"abstract":"<p><strong>Introduction: </strong>Vascular smooth muscle cells (VSMCs)-based foam cell formation is a crucial factor in the atherosclerosis process. We aimed to explore the mechanism of Golgi a-mannosidase II (GMII) effects on the VSMCs-based foam cell formation.</p><p><strong>Material and methods: </strong>VSMCs were exposed to different concentrations of low-density lipoproteins (LDLs), lipopolysaccharide (LPS), and/or GMII inhibitor (swainsonine). The qRT-PCR and western blot were used for expression analysis. Oil Red O staining was used to verify changes of lipid droplets in VSMCs. The translocation of the SCAP from the endoplasmic reticulum (ER) to Golgi was detected by immunofluorescence (IF).</p><p><strong>Results: </strong>LPS disrupted the LDLs-mediated regulation of LDL receptor (LDLr) and increased intracellular cholesterol ester, which was inversely inhibited by swainsonine. The activity of a-mannosidase II and GMII expression were decreased by LDLs but increased by the addition of LPS. Conversely, LPS-induced enhancement was reversed by swainsonine. Additionally, swainsonine reversed the LPS-induced increase of intracellular lipid droplets in the presence of LDLs. Expression analysis demonstrated that LDLr, SCAP, and SREBP2 were up-regulated by LPS, but reversed by swainsonine in LDLs-treated cells. IF staining revealed that swainsonine inhibited the translocation of SCAP to Golgi under inflammatory stress.</p><p><strong>Conclusions: </strong>Collectively, swainsonine restrained LDLr expression to suppress the formation of VSMCs-based foam cells by reducing SREBP2 and SCAP under inflammatory stress conditions, suggesting that GMII contributes to the formation of VSMCs-based foam cells under inflammatory stress.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"59 2","pages":"134-143"},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39258152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keratin 17 knockdown suppressed malignancy and cisplatin tolerance of bladder cancer cells, as well as the activation of AKT and ERK pathway.","authors":"Chen Li,&nbsp;HongWei Su,&nbsp;CongGang Ruan,&nbsp;XiangDong Li","doi":"10.5603/FHC.a2021.0005","DOIUrl":"https://doi.org/10.5603/FHC.a2021.0005","url":null,"abstract":"<p><strong>Introduction: </strong>Bladder cancer (BCa) is one the most common urinary system malignancies and approximately one quarter of diagnosis is invasive muscle-invasive BCa. Accumulating evidence revealed that keratin 17 (KRT17) is closely related to the prognosis and progression of various tumors including a recent study also implying the potential role of KRT17 in the diagnosis of BCa. However, the specific role of KRT17 in BCa remains to be elucidated.</p><p><strong>Material and methods: </strong>The expression of KRT17 in 5637 BCa cells and SV-HUC-1 normal human urothelial cells was detected using quantitative real-time PCR (qRT-PCR) and western blot. Short hairpin RNA targeting KRT17 was used to knockdown KRT17 in BCa cells. The colony formation was assessed and the proliferation of cells was studied by Cell Counting Kit-8 (CCK-8). Invasion and epithelial-mesenchymal transition (EMT) capacity of BCa cells were assessed using transwell assay and western blot, respectively. Cisplatin sensitivity of cancer cells was measured by evaluating the cell viability using CCK-8 assay. The downstream pathway of KRT17 was explored by western blot.</p><p><strong>Results: </strong>The expression of KRT17 was elevated in BCa cells in comparison with the normal human urothelial cell at the mRNA and protein levels. The in vitro assays demonstrated that KRT17 interference affected the proliferation, colony formation and invasion capacity of BCa cells, as well as EMT. Furthermore, knockdown of KRT17 enhanced cisplatin sensitivity in BCa cells. Mechanically, KRT17 ablation led to the inactivation of both AKT and ERK pathways.</p><p><strong>Conclusions: </strong>Our results elucidate the vital role of KRT17 in the development of malignancy of BCa cells, probably by the activation of AKT and ERK pathways and suggest that it may represent a novel therapeutic target for BCa.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"59 1","pages":"40-48"},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25362417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth factors in the initial stage of bone formation, analysis of their expression in chondrocytes from epiphyseal cartilage of rat costochondral junction.","authors":"Anna Hyc,&nbsp;Stanislaw Moskalewski,&nbsp;Anna Osiecka-Iwan","doi":"10.5603/FHC.a2021.0017","DOIUrl":"https://doi.org/10.5603/FHC.a2021.0017","url":null,"abstract":"<p><strong>Introduction: </strong>In endochondral ossification septoclasts and osteoclasts (also called chondroclasts) release growth factors deposited in non-calcified and calcified zones of the growth plate. They stimulate, within the metaphysis, initial stages of the bone formation. We have recently reported quantitation of several growth factors in calcified cartilage from calf costochondral junction. Data from the analogous human cartilage could possibly help to choose efficient combinations of growth factors for clinical applications, but the amount of the calcified cartilage needed for analysis of numerous growth factors would be difficult to collect. The estimation of growth factors expression in endochondral chondrocytes may, indirectly, indicate which of them play a leading role in the stimulation of osteoprogenitor cells in metaphysis. To test this hypothesis, we used rat chondrocytes to evaluate mRNA levels of several growth factors.</p><p><strong>Materials and methods: </strong>Chondrocytes were isolated from proliferative and hypertrophic zones of the epiphyseal cartilage forming costochondral junctions of inbred Lewis rats. The total RNA was isolated from chondrocytes and the level of mRNA for bone morphogenetic proteins 1-7 (BMP-1-7), vascular endothelial growth factor A (VEGF-A), basic fibroblast growth factor (bFGF), growth/differentiation factor 5 (GDF-5), NEL-like protein 1 (NELL-1), transforming growth factor beta 1 (TGF-b1), mesencephalic astrocyte-derived neurotrophic factor (MANF), connective tissue growth factor (CTGF), osteoclast-stimulating factor 1 (OSTF-1) and insulin-like growth factor 1 (IGF-1) was evaluated using real-time PCR method.</p><p><strong>Results: </strong>All studied factors were expressed. The highest level of mRNA was detected for CTGF, MANF, VEGF-A and TGF-b1. Expression was also quite high for BMP-1, BMP-2, BMP-5, BMP-6, BMP-7, IGF-1, GDF-5 and OSTF-1. Very low level of mRNA was detected for BMP-3, BMP-4 and NELL-1.</p><p><strong>Conclusions: </strong>Chondrocytes from the proliferative and hypertrophic zones of the growth plate produce factors involved in the cartilage metabolism and bone formation. The determination of these growth factors in humans could help to choose their optimal composition necessary for stimulation of bone formation in clinical practice. In rat the best stimulation of bone formation would presumably be achieved with a mixture of BMP-2, BMP-5, BMP-6 and BMP-7.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"59 3","pages":"178-186"},"PeriodicalIF":1.5,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39260177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Professor Stanisława Stokłosowa (1927-2019).","authors":"B. Bilińska, Jerzy Galas, A. Lukaszyk, J. Kawiak","doi":"10.5603/FHC.a2019.0021","DOIUrl":"https://doi.org/10.5603/FHC.a2019.0021","url":null,"abstract":"","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"57 4 1","pages":"155-156"},"PeriodicalIF":1.5,"publicationDate":"2020-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44281765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bisdemethoxycurcumin exerts a cell-protective effect via JAK2/STAT3 signaling in a rotenone-induced Parkinson's disease model in vitro.","authors":"Duanqun He,&nbsp;Shuangxi Chen,&nbsp;Zijian Xiao,&nbsp;Heng Wu,&nbsp;Guijuan Zhou,&nbsp;Chenlin Xu,&nbsp;Yunqian Chang,&nbsp;Yihui Li,&nbsp;Gang Wang,&nbsp;Ming Xie","doi":"10.5603/FHC.a2020.0011","DOIUrl":"https://doi.org/10.5603/FHC.a2020.0011","url":null,"abstract":"<p><strong>Introduction: </strong>Oxidative stress and cell apoptosis have both been suggested to be closely associated with the pathogenesis of Parkinson's disease (PD). Previously, bisdemethoxycurcumin (BDMC) has been shown to exhibit several desirable characteristics as a candidate neuroprotective agent, including antioxidant and anti-inflammatory activities in the nervous system. However, whether BDMC can exert cell-protective roles in an in vitro model of PD remains unknown.</p><p><strong>Material and methods: </strong>SH-SY5Y cells were pretreated with BDMC, with or without AG490 and SI-201, for 30 min, followed by a co-incubation with rotenone for 24 h. Subsequently, a cell viability assay and western blotting was performed, and SOD and GSH activities were analyzed.</p><p><strong>Results: </strong>The results revealed that the pretreatment with BDMC enhanced the cell survival, antioxidative stress capacity and the phosphorylation levels of JAK/STAT3 in SH-SY5Y cells treated with rotenone. However, following the incubation with AG490 and SI-201, inhibitors of the JAK/STAT3 signaling pathway, BDMC was unable to exert cell-protective roles in SH-SY5Y cells treated with rotenone.</p><p><strong>Conclusions: </strong>In conclusion, the results suggested that BDMC may exert a cell-protective role in SH-SY5Y cells in vitro via JAK2/STAT3 signaling, thus suggesting the possible application of BDMC for the treatment of neurodegenerative diseases related to JAK2/STAT3 signaling.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"58 2","pages":"127-134"},"PeriodicalIF":1.5,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38060359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IGFBP7 aggravates sepsis-induced acute lung injury by activating the ERK1/2 pathway.","authors":"Qiaolian Xu,&nbsp;Jun Wang","doi":"10.5603/FHC.a2020.0028","DOIUrl":"https://doi.org/10.5603/FHC.a2020.0028","url":null,"abstract":"<p><strong>Introduction: </strong>Sepsis is characterized by an infection-caused acute inflammatory response, which is usually accompanied by multiple organ failure, especially lung injury. During sepsis, a large number of endotoxins such as lipopolysaccharides (LPSs) are secreted from Gram-negative bacteria. However, the mechanisms underlying acute lung dysfunction caused by sepsis have not yet been well defined.</p><p><strong>Material and methods: </strong>To identify the mechanism of insulin-like growth factor binding protein 7 (IGFBP7) in acute lung injury during sepsis, the effects of IGFBP7 shRNA were evaluated in a model of cecal ligation puncture (CLP)-induced sepsis in mice. Histologic evaluation of the effects of IGFBP7 on CLP-induced acute lung injury was performed by H&E staining. Murine pulmonary microvascular endothelial cells (MPVECs) were transfected with shIGFBP7 or shNC before treatment with LPS to mimic the sepsis-induced lung dysfunction. The effects of CLP or LPS on IGFBP7 expression and the activation of ERK1/2 pathway were analyzed by western blot. MTT and LDH assays were used to measure the viability of MPVECs under different treatment regimes. The apoptosis rate of MPVECs in different groups was detected by flow-cytometry analysis.</p><p><strong>Results: </strong>IGFBP7 was strongly up-regulated in sepsis-induced acute lung injury in mice. IGFBP7 silencing attenuated sepsis-induced apoptosis and cytotoxicity in MPVECs. Furthermore, the activation of ERK1/2 pathway was regulated by IGFBP7 during sepsis-induced inflammation. IGFBP7 inhibition by RNA interference in MPVECs attenuated CLP-induced morphological features of lung dysfunction. The knockdown of IGFBP7 attenuated LPS-induced MPVECs' apoptosis by the suppression of the ERK1/2 pathway.</p><p><strong>Conclusions: </strong>We demonstrated for the first time that IGFBP7 is involved in the pathogenesis of sepsis-induced acute lung injury and may serve as a therapeutic target in sepsis-induced acute lung injury.</p>","PeriodicalId":12322,"journal":{"name":"Folia histochemica et cytobiologica","volume":"58 4","pages":"247-254"},"PeriodicalIF":1.5,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38378609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}