SDF-1/CXCR4 axis promotes osteogenic differentiation of BMSCs through the JAK2/STAT3 pathway.

IF 1.7 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Folia histochemica et cytobiologica Pub Date : 2021-01-01 Epub Date: 2021-09-28 DOI:10.5603/FHC.a2021.0020

Wen Xiong, Xin Guo, Xianhua Cai

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引用次数: 0

Abstract

Introduction: This study aimed to investigate the effects of stromal cell-derived factor-1 (SDF-1) and activation of its receptor, chemokine receptor 4 (CXCR4), on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and the key signaling mechanisms involved in these effects.

Material and methods: BMSCs were treated with 100 μg/L SDF-1 and cultured in osteogenic medium for 7 days. RT-qPCR and western blotting were used to detect the protein and mRNA levels of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), Runt-related transcription factor 2 (Runx2), and osteocalcin (OCN). Alizarin-red staining was used to detect the mineralization-inducing ability of the cells.

Results: After BMSCs were treated with SDF-1, the levels of JAK2 mRNA, STAT3 mRNA, and protein phosphorylation increased, the number of mineralized nodules of BMSCs increased, and the osteogenic-differentiation ability was enhanced. In addition, after BMSCs were treated with an inhibitor of JAK2 phosphorylation, the levels of JAK2, STAT3, Runx2, and OCN decreased significantly, the number of mineralized nodules of BMSCs also decreased, and the osteogenic-differentiation ability decreased. The inhibition of CXCR4-treated BMSCs further confirmed that SDF-1/CXCR4 activated JAK2/STAT3 to regulate the osteogenic differentiation of BMSCs.

Conclusions: SDF-1/CXCR4 promoted the osteogenic differentiation of BMSCs through JAK2/STAT3 activation.

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SDF-1/CXCR4轴通过JAK2/STAT3通路促进BMSCs成骨分化。

本研究旨在探讨基质细胞衍生因子-1 (SDF-1)及其受体趋化因子受体4 (CXCR4)的激活对骨髓间充质干细胞(BMSCs)成骨分化的影响，以及参与这些影响的关键信号机制。材料与方法:用100 μg/L SDF-1处理骨髓间充质干细胞，在成骨培养基中培养7 d。采用RT-qPCR和western blotting检测Janus kinase 2 (JAK2)、信号转导和转录激活因子3 (STAT3)、runt相关转录因子2 (Runx2)和骨钙素(OCN)的蛋白和mRNA水平。采用茜素红染色检测细胞的矿化诱导能力。结果:经SDF-1处理后，骨髓间充质干细胞JAK2 mRNA、STAT3 mRNA水平及蛋白磷酸化水平升高，矿化结节数量增加，成骨分化能力增强。此外，用JAK2磷酸化抑制剂处理骨髓间充质干细胞后，JAK2、STAT3、Runx2、OCN水平显著降低，骨髓间充质干细胞矿化结节数量减少，成骨分化能力下降。抑制CXCR4处理的骨髓间充质干细胞进一步证实了SDF-1/CXCR4激活JAK2/STAT3调控骨髓间充质干细胞成骨分化。结论:SDF-1/CXCR4通过激活JAK2/STAT3促进BMSCs成骨分化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia histochemica et cytobiologica 生物-生化与分子生物学

CiteScore

2.80

自引率

6.70%

发文量

审稿时长

6-12 weeks

期刊介绍： "Folia Histochemica et Cytobiologica" is an international, English-language journal publishing articles in the areas of histochemistry, cytochemistry and cell & tissue biology. "Folia Histochemica et Cytobiologica" was established in 1963 under the title: ‘Folia Histochemica et Cytochemica’ by the Polish Histochemical and Cytochemical Society as a journal devoted to the rapidly developing fields of histochemistry and cytochemistry. In 1984, the profile of the journal was broadened to accommodate papers dealing with cell and tissue biology, and the title was accordingly changed to "Folia Histochemica et Cytobiologica". "Folia Histochemica et Cytobiologica" is published quarterly, one volume a year, by the Polish Histochemical and Cytochemical Society.