Actinidia chinensis Planch. root extract inhibits the proliferation, migration and invasion of breast cancer cells via the AKT/GSK-3β signaling pathway.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2021-01-01 Epub Date: 2021-12-01 DOI:10.5603/FHC.a2021.0023

Chunchun Gan, Zhan Jin, Xiaopeng Wei, Meina Jin

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引用次数: 3

Abstract

Introduction: Actinidia chinensis Planch. root extract (acRoots), known as a traditional Chinese medicine (TCM), has shown antitumor efficacy in various types of human cancers. However, its role and underlying mechanisms in breast cancer (BCa) have not been elucidated.

Material and methods: In the present study, the effects of acRoots on cell viability, apoptosis, migration and invasion were analyzed by MTT assay, colony formation, flow cytometry, wound healing and Transwell assay in MDA-MB-231 and MDA-MB-453 breast cancer cell lines. The expression levels of relevant proteins were determined by Western blot assay.

Results: The results revealed that acRoots inhibited proliferation, migration, and invasion and promoted apoptosis of BCa cells. Moreover, acRoots decreased the expression of cyclin D1, survivin, Bcl-2, N-cadherin, and Snail and increased the expression of Bax and E-cadherin in MDA-MB-231 and MDA-MB-453 cells. AcRoots inhibited the AKT/GSK-3b pathway by decreasing the levels of phosphorylated AKT, phosphorylated GSK-3b and b-catenin.

Conclusions: The described effects of acRoots on the cultured BCa cells suggest that they may be mediated by the inhibition of the AKT/GSK-3b signaling pathway. Thus, further studies are warranted to test the possibility that AcRoots may be used as a promising anticancer agent for breast cancer treatment.

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猕猴桃。根提取物通过AKT/GSK-3β信号通路抑制乳腺癌细胞的增殖、迁移和侵袭。

简介:猕猴桃。根提取物(acRoots)是一种传统的中药(TCM)，在各种类型的人类癌症中显示出抗肿瘤功效。然而，其在乳腺癌(BCa)中的作用和潜在机制尚未阐明。材料与方法:本研究采用MTT法、集落形成法、流式细胞术、创面愈合法和Transwell法对MDA-MB-231和MDA-MB-453乳腺癌细胞株的细胞活力、凋亡、迁移和侵袭进行了分析。Western blot法检测相关蛋白的表达水平。结果:acRoots能抑制BCa细胞的增殖、迁移和侵袭，促进BCa细胞的凋亡。此外，acRoots降低了MDA-MB-231和MDA-MB-453细胞中cyclin D1、survivin、Bcl-2、N-cadherin和Snail的表达，增加了Bax和E-cadherin的表达。AcRoots通过降低磷酸化AKT、磷酸化GSK-3b和b-catenin的水平抑制AKT/GSK-3b通路。结论:acRoots对培养BCa细胞的作用提示其可能通过抑制AKT/GSK-3b信号通路介导。因此，有必要进行进一步的研究，以测试AcRoots作为一种有前景的乳腺癌治疗抗癌药物的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.