FEMS microbiology ecology最新文献_第7页

Host-selective PMA-PCR enhances bacterial diversity recovery and lowers detection limits in plant microbiome profiling. 宿主选择性PMA-PCR增强了细菌多样性恢复，降低了植物微生物组分析的检测限。

IF 4.1 3区生物学

FEMS microbiology ecology Pub Date : 2026-06-18 DOI: 10.1093/femsec/fiag071

Anatolijs Bervjačonoks, Antonija Rimša, Ivars Anspoks, Ance Roga, Megija Luņģe, Dita Gudrā, Anitra Zīle, Elza Kaktiņa, Jevgenija Ņečajeva, Davids Fridmanis, Anete Borodušķe

{"title":"Host-selective PMA-PCR enhances bacterial diversity recovery and lowers detection limits in plant microbiome profiling.","authors":"Anatolijs Bervjačonoks, Antonija Rimša, Ivars Anspoks, Ance Roga, Megija Luņģe, Dita Gudrā, Anitra Zīle, Elza Kaktiņa, Jevgenija Ņečajeva, Davids Fridmanis, Anete Borodušķe","doi":"10.1093/femsec/fiag071","DOIUrl":"10.1093/femsec/fiag071","url":null,"abstract":"Co-amplification of host DNA remains a significant obstacle in plant microbiome profiling, as universal taxonomic markers, such as the 16S rRNA gene, are also present in plant organellar genomes. While strategies like blocking primers can enrich bacterial reads, they rely on prior sequence knowledge and may introduce bias. We developed a sequence-independent approach that suppresses host DNA amplification by combining physical pretreatments that selectively compromise plant cell integrity with propidium monoazide (PMA) treatment, which binds exposed DNA and prevents its PCR amplification. We observed that various pretreatments-including cryopreservation and mechanical disruption enhanced plant cell susceptibility to PMA without affecting bacterial cells. The applied approach resulted in significantly increased proportions of bacterial reads and improved detection of bacterial diversity in downstream NGS. Compared to sequence-dependent strategies such as blocking primers, this method achieved comparable or superior performance in reducing host DNA interference-particularly in low-biomass samples. Our results establish cryopreservation-enhanced PMA-PCR as a robust, sequence-independent method for high-resolution plant microbiome profiling.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13355530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148375070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolism of β-mannans by representative, understudied Bacillota species from the human colon. 人类结肠中代表性的、未被充分研究的杆菌种对β-甘露聚糖的代谢。

IF 4.1 3区生物学

FEMS microbiology ecology Pub Date : 2026-06-18 DOI: 10.1093/femsec/fiag063

Galiana Lo, Sabina Leanti La Rosa, Bjørge Westereng, Phillip B Pope, Petra Louis, Carlos Sabater, Sylvia H Duncan

{"title":"Metabolism of β-mannans by representative, understudied Bacillota species from the human colon.","authors":"Galiana Lo, Sabina Leanti La Rosa, Bjørge Westereng, Phillip B Pope, Petra Louis, Carlos Sabater, Sylvia H Duncan","doi":"10.1093/femsec/fiag063","DOIUrl":"10.1093/femsec/fiag063","url":null,"abstract":"The macronutrients in our diets including non-starch polysaccharides such as β-mannans, found in plant cell walls, can impact on human gut health. There is however a paucity of data regarding the ability of gut bacteria, in particular those belonging to the Bacillota (previously Firmicutes) phylum, to depolymerise and ferment β-mannans. In this study, we tested a total of 12 strains, including nine Bacillota, for their ability to metabolise and cross-feed on β-mannans. Three of the six butyrate-producing Bacillota strains, namely Roseburia intestinalis L1-82, Roseburia faecis M72/1, and Coprococcus eutactus ART55/1, were able to metabolise carob galactomannan, konjac glucomannan, and softwood spruce acetylated galactoglucomannan, which corresponded with their carbohydrate active enzyme profiles, whilst Faecalibacterium prausnitzii S3L/3 only grew well on β-mannan endo-mannanase digests. To investigate competition and microbial cross-feeding on β-mannans, growth assays were conducted with co-cultures of up to six strains belonging to both the Bacillota phylum and a Bacteroidetes β-mannan utilising strain, Bacteroides ovatus V975. All strains in the mixes were able to co-exist, including the non-mannan degrading butyrate producers, with butyrate being formed as one of the major fermentation products. These studies suggest that β-mannans may offer a notable prebiotic approach to promoting butyrate-producing bacteria and gut health.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13278490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148224163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon monoxide oxidizers in soils of different ages from Piton de la Fournaise volcano. 皮顿德拉富尔奈斯火山不同年代土壤中的一氧化碳氧化剂。

IF 4.1 3区生物学

FEMS microbiology ecology Pub Date : 2026-06-18 DOI: 10.1093/femsec/fiag062

Constance Wildbur, Robin A Dawson, Shamik Roy, Claudine Ah-Peng, Mikk Espenberg, Marcela Hernández

{"title":"Carbon monoxide oxidizers in soils of different ages from Piton de la Fournaise volcano.","authors":"Constance Wildbur, Robin A Dawson, Shamik Roy, Claudine Ah-Peng, Mikk Espenberg, Marcela Hernández","doi":"10.1093/femsec/fiag062","DOIUrl":"10.1093/femsec/fiag062","url":null,"abstract":"Volcanic soils provide a unique environment for studying microbial colonization and succession due to their extreme conditions and distinct geochemical profiles. This study focused on carbon monoxide (CO)-oxidizing microbial communities in volcanic soils at Piton De La Fournaise, Réunion Island. Soil samples from three sites (corresponding to eruptions in 1401, 1559, and 2007) were analysed to assess microbial community structure using 16S rRNA gene sequencing and metagenomic analysis to identify functional genes involved in CO oxidation. Phylum-level analysis showed higher relative abundance of Acidobacteriota and Chloroflexota, lower abundances of Actinomycetota and Bacteroidota, and relatively stable levels of Pseudomonadota, while class-level patterns included rising Alphaproteobacteria and Acidobacteriia, with Ktenobacteria emerging in the 1401 site. CO dehydrogenase-related genes were found in 17 metagenome-assembled genomes across all sites. The CO consumption rate by microbes in soils was measured. CO-oxidizing microbes were present across soil ages, with detectable activity in the 2007 site and greatest activity in the 1401 site, suggesting that these microbes actively use CO as an energy source even in soils with primary vegetation, contrary to general understanding. The findings suggest intricate dynamics of microbial succession in volcanic soils and may challenge conventional expectations about community complexity over time.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13285871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148210800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrogenic CH4 oxidation on a bioanode: putative extracellular electron transport system in Methylobacter sp. 甲基杆菌胞外电子传递系统在生物阳极上的电致CH₄氧化。

IF 4.1 3区生物学

FEMS microbiology ecology Pub Date : 2026-06-18 DOI: 10.1093/femsec/fiag067

Peter A G Ter Horst, Ian P G Marshall, Reinier A Egas, Robin Klomp, Merijn A W Schutgens, Theo van Alen, Mike S M Jetten, Caroline P Slomp, Cornelia U Welte

{"title":"Electrogenic CH4 oxidation on a bioanode: putative extracellular electron transport system in Methylobacter sp.","authors":"Peter A G Ter Horst, Ian P G Marshall, Reinier A Egas, Robin Klomp, Merijn A W Schutgens, Theo van Alen, Mike S M Jetten, Caroline P Slomp, Cornelia U Welte","doi":"10.1093/femsec/fiag067","DOIUrl":"10.1093/femsec/fiag067","url":null,"abstract":"Aerobic methanotrophs are frequently detected in oxygen-limited, stratified coastal environments. Known adaptations, including high-affinity terminal oxidases and oxygen-binding bacteriohemerythrins, help explain methane oxidation at extremely low oxygen concentrations, yet their activity and ecological role under fully anoxic conditions remain uncertain. Here, we show that an anoxic, poised-anode bioelectrochemical system inoculated with a methane-oxidizing sediment enrichment produced methane-dependent current, with rapid current loss upon methane removal and recovery after re-addition. Metagenomic analysis revealed the selective enrichment of a Methylobacter population encoding a porin-cytochrome complex and numerous multiheme c-type cytochromes, suggesting extracellular electron transfer potential. A complementary phylogenomic survey across Methylococcales identified homologs of this gene cluster in multiple lineages, but with a scattered phylogenetic distribution indicative of modular acquisition. Comparative synteny further revealed conserved gene order across genomes, supporting horizontal transfer of the locus as a functional unit. Together, these results demonstrate that aerobic methanotrophs may employ extracellular electron transfer strategies to dissipate methane-derived electrons when oxygen-dependent respiration is constrained.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13322032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148307344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional genetic potential of benthic microbial mat communities in Arctic, Antarctic, and sub-Antarctic lakes. 北极、南极和亚南极湖泊底栖微生物群落的功能遗传潜力

IF 4.1 3区生物学

FEMS microbiology ecology Pub Date : 2026-06-18 DOI: 10.1093/femsec/fiag060

Jill De Visscher, Bjorn Tytgat, Dominic A Hodgson, Annick Wilmotte, Anne Willems, Elie Verleyen, Wim Vyverman

{"title":"Functional genetic potential of benthic microbial mat communities in Arctic, Antarctic, and sub-Antarctic lakes.","authors":"Jill De Visscher, Bjorn Tytgat, Dominic A Hodgson, Annick Wilmotte, Anne Willems, Elie Verleyen, Wim Vyverman","doi":"10.1093/femsec/fiag060","DOIUrl":"10.1093/femsec/fiag060","url":null,"abstract":"Benthic microbial mat communities are key drivers of ecosystem functioning in polar lakes and ponds, forming the base of aquatic food webs and contributing substantially to nutrient cycling. Although Arctic, sub-Antarctic, and Antarctic microbial mats differ in community composition, their functional genetic potential remains poorly understood. We applied shotgun metagenomic sequencing to study 17 microbial mat communities from Arctic and (sub-)Antarctic lakes differing in salinity, catchment vegetation, and climatic conditions. Stress response genes, especially cold stress, and phosphorus cycling and metabolism genes were highly abundant in all lakes. A large proportion of functional genes was shared between regions, with core functions dominated by transport mechanisms and energy production. However, clear differences in particular gene abundances were observed. Several East-Antarctic lakes and inland ponds in the Transantarctic Mountains showed a dominance of oxygenic photosynthesis and Calvin cycle genes for carbon fixation, likely reflecting the dominance of Cyanobacteriota. In Arctic and sub-Antarctic lakes with catchment vegetation and higher arthropod abundances, lignin and chitin degradation genes were more important. Our study shows that, despite distinct biogeographic patterns in community composition, the functional genetic potential of polar lake microbial mats mainly reflects climatic and local environmental conditions, emphasizing specific adaptations to extreme polar environments.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13278500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148249656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Species level composition of Faecalibacterium spp. in the gut of Japanese adults revealed by rpoA-based sequencing analysis. 基于rpoa的序列分析揭示了日本成人肠道中Faecalibacterium spp.的物种水平组成。

IF 4.1 3区生物学

FEMS microbiology ecology Pub Date : 2026-05-21 DOI: 10.1093/femsec/fiag049

Adeline Ang, Eri Mitsuyama, Hiroki Kaneko, Toshitaka Odamaki, Akihito Endo

{"title":"Species level composition of Faecalibacterium spp. in the gut of Japanese adults revealed by rpoA-based sequencing analysis.","authors":"Adeline Ang, Eri Mitsuyama, Hiroki Kaneko, Toshitaka Odamaki, Akihito Endo","doi":"10.1093/femsec/fiag049","DOIUrl":"10.1093/femsec/fiag049","url":null,"abstract":"Faecalibacterium is a predominant genus in the human gut that potentially supports intestinal and systemic health. Recent genomic studies revealed heterogeneity within the genus, leading to the recognition of additional species beyond the type species, Faecalibacterium prausnitzii. Despite its biological importance, the species-level distribution and ecological diversity of Faecalibacterium spp. remains poorly characterized, partly due to methodological constraints. Here, we developed a rpoA-based primer set specific to the Faecalibacterium genus and applied on fecal samples from 580 healthy Japanese adults for species-level profiling with next-generation sequencing. Our results revealed clear distinctions in species-level population, as well as inter-individual variations influenced by host characteristics and dietary factors. The majority of Faecalibacterium composition consisted of F. longum, F. taiwanense, F. duncaniae, F. prausnitzii, and a yet-to-be-classified species (Group 11). Notably, F. prausnitzii was neither the most prevalent nor the most abundant species. Besides that, host driven differences such as body mass index revealed negative association between relative abundance of F. duncaniae and Group 11. Additionally, Group 11 displayed lower relative abundance in lower caloric intake group. Overall, these findings highlight environment- and host-dependent shifts in Faecalibacterium populations, revealing species-specific ecological adaptations and potential physiological roles in human health.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13214989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147863870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling underlying mechanisms and responsible microbes of organic residue-stimulated atmospheric methane uptake in agricultural soils. 揭示有机残留物刺激农业土壤中大气甲烷吸收的潜在机制和负责微生物。

IF 4.1 3区生物学

FEMS microbiology ecology Pub Date : 2026-05-21 DOI: 10.1093/femsec/fiag048

Stijn G van den Bergh, Iris Chardon, Marion Meima-Franke, Gabriel S Rocha, Xiaoyu Cheng, Ciska Raaijmakers, Wietse de Boer, Paul L E Bodelier

{"title":"Unraveling underlying mechanisms and responsible microbes of organic residue-stimulated atmospheric methane uptake in agricultural soils.","authors":"Stijn G van den Bergh, Iris Chardon, Marion Meima-Franke, Gabriel S Rocha, Xiaoyu Cheng, Ciska Raaijmakers, Wietse de Boer, Paul L E Bodelier","doi":"10.1093/femsec/fiag048","DOIUrl":"10.1093/femsec/fiag048","url":null,"abstract":"It has been reported that compost amendment improves atmospheric CH4 uptake of agricultural soils. However, microbes involved as well as the underlying mechanisms responsible for the observed effect remain unclear. Here we identified active methane-oxidizing bacteria (MOB) at (circum-) atmospheric CH4 concentrations in agricultural soils amended with green compost, and investigated three complementary hypotheses: (i) atmospheric CH4 consumption is driven by highly activated, flush-feeding MOB; (ii) stimulation of internal CH4 production which fuels flush-feeding methanotrophic activity; and (iii) increased availability of H2 that can serve as additional energy source for mixotrophic methanotrophy. First, we showed that MOB previously activated by exposure to high CH4 concentrations can subsequently oxidize atmospheric CH4 via the flush-feeding lifestyle. Second, no internal CH4 production in soil was observed following compost amendment, likely due to lack of suitable substrates for methanogenesis. Third, provision of elevated H2 concentrations did not affect the concurrent atmospheric CH4 oxidation. Phospholipid fatty acid-stable isotope probing revealed that four distinct MOB groups were active at (circum-) atmospheric CH4 concentrations in agricultural soils and green compost: Methylocaldum sp., Methylosinus sporium, Methylocystis sp./Methylosinus trichosporium, and USCα. These findings enhance our understanding of methanotroph ecology and can be used to craft more effective strategies of creating \"climate-smart\" soils.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13214991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ghost-rocks' microbiota: metagenomic insights into their influence on the biogeochemistry of karstic cave and groundwater. 鬼岩微生物群：对溶洞和地下水生物地球化学影响的宏基因组研究。

IF 4.1 3区生物学

FEMS microbiology ecology Pub Date : 2026-05-21 DOI: 10.1093/femsec/fiag047

Guillaume Peugnet, Célie Pisapia, Bénédicte Ménez, Maud Watkinson, Léna Lecourt, Nicolas Peugnet, Julien Bouchez, Laurent Bruxelles, Emmanuelle Gérard

{"title":"Ghost-rocks' microbiota: metagenomic insights into their influence on the biogeochemistry of karstic cave and groundwater.","authors":"Guillaume Peugnet, Célie Pisapia, Bénédicte Ménez, Maud Watkinson, Léna Lecourt, Nicolas Peugnet, Julien Bouchez, Laurent Bruxelles, Emmanuelle Gérard","doi":"10.1093/femsec/fiag047","DOIUrl":"10.1093/femsec/fiag047","url":null,"abstract":"Microbial communities in the critical zone drive key geochemical processes, but many subsurface habitats remain poorly characterized. Ghost-rock karst systems in particular represent unexplored microbial niches. Here, we provide the first genome-resolved metagenomic comparison of ghost-rock and groundwater microbial communities from the Sterkfontein karst system (South Africa). Ghost-rock and groundwater communities host distinct taxonomic and metabolic assemblages. Groundwater communities are dominated by chemolithotrophs capable of oxidizing sulfur- and nitrogen-bearing compounds, and by heterotrophs degrading refractory, plant-derived organic matter. In contrast, primary producers in ghost-rocks likely rely on atmospheric chemosynthesis via trace gas oxidation, while glycogen metabolism and necromass recycling point to adaptations to oligotrophic and fluctuating hydrological conditions. Groundwater taxa with metal-interacting pathways may initiate bedrock colonization via metal oxidation, whereas ghost-rock communities include potential metal reducers that could drive iron and manganese oxide dissolution and influence trace element mobility. Together, these results underscore ghost-rocks as active microbial and geochemical hot spots within karst systems that may play a non-negligible role on biomineralization/bioweathering processes and on shaping (sub)terrestrial landscapes and global biogeochemical cycles.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13264449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148217208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature at parental generation affects bacterial communities associated with offspring for both host and parasitoid. 亲代温度对寄主和拟寄生物后代相关的细菌群落都有影响。

IF 4.1 3区生物学

FEMS microbiology ecology Pub Date : 2026-05-21 DOI: 10.1093/femsec/fiag046

Camila Souza Beraldo, Diego Castillo Franco, Saskya van Nouhuys, Anne Duplouy

{"title":"Temperature at parental generation affects bacterial communities associated with offspring for both host and parasitoid.","authors":"Camila Souza Beraldo, Diego Castillo Franco, Saskya van Nouhuys, Anne Duplouy","doi":"10.1093/femsec/fiag046","DOIUrl":"10.1093/femsec/fiag046","url":null,"abstract":"The thermal conditions experienced during development can affect host-associated microbial communities. We still know little about whether such effects similarly persist across life stages between different species. In particular, it is unclear if the bacterial communities of closely interacting species, such as hosts and their endoparasitoids, exhibit similar responses to thermal conditions. We reared two generations of the Melitaea cinxia butterfly and its specialised parasitoid wasp, Hyposoter horticola, at three temperatures in the laboratory (26°C, 28°C, and 31°C). We found that the two species harbour different bacterial communities as adults, with the parasitoid exhibiting higher bacterial richness than its host butterfly. When the parental generation of the butterfly was exposed to high temperatures, the F1 generation exhibited increased bacterial richness but a reduced diversity (Shannon index). The opposite effect was observed for its parasitoid, but only for the wasps infected with Wolbachia, which appears sensitive to thermal conditions. Collectively, these results highlight that the bacterial communities of insect hosts and their parasitoids are distinct units, differently susceptible to environmental thermal conditions, particularly to temperatures experienced at the parental generation.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13192552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arbuscular mycorrhizal fungi- linked microbial processes in soil nitrogen cycling. 丛枝菌根真菌在土壤氮循环中的相关微生物过程。

IF 4.1 3区生物学

FEMS microbiology ecology Pub Date : 2026-05-21 DOI: 10.1093/femsec/fiag050

Anukool Vaishnav, Jan Jansa

{"title":"Arbuscular mycorrhizal fungi- linked microbial processes in soil nitrogen cycling.","authors":"Anukool Vaishnav, Jan Jansa","doi":"10.1093/femsec/fiag050","DOIUrl":"10.1093/femsec/fiag050","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) create an extended plant-soil interface (referred to as mycorrhizal hyphosphere) where specific microbial interactions shape key steps of the nitrogen (N) cycle. Extraradical hyphae of the AMF host diverse microbes that help mineralize organic substrates, regulate ammonium and nitrate transformations, and enhance N retention. These processes allow plants to access both inorganic N and the N released from complex organic compounds. This review synthesizes current evidence for AMF-microbe interactions in relation to mineralization, nitrification, denitrification, and (di)nitrogen fixation. It also highlights unresolved questions, such as when AMF transition from facilitating to competing for N, how they access stabilized organic N pools, and how the carbon: nitrogen: phosphorus (C: N: P) nutrient stoichiometry of the soil environment constrain AMF-mediated N transfer to plants. Furthermore, we discuss how AMF-centered pathways can contribute to more circular N flows in agroecosystems by promoting tighter internal N cycling through microbial immobilization, turnover, and subsequent transfer to plants via AMF hyphae. By integrating spatial, microbial, and stoichiometric perspectives, this review provides a mechanistic framework for understanding AMF-driven N dynamics and their potential role in enhancing N use efficiency in managed and natural systems.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13201079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147874082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0