Extremophiles最新文献

{"title":"Halorussus vallis sp. nov., Halorussus aquaticus sp. nov., Halorussus gelatinilyticus sp. nov., Halorussus limi sp. nov., Halorussus salilacus sp. nov., Halorussus salinisoli sp. nov.: six extremely halophilic archaea isolated from solar saltern, salt lake and saline soil.","authors":"Xi-Wen Zheng,&nbsp;Zhang-Ping Wu,&nbsp;Ya-Ping Sun,&nbsp;Bei-Bei Wang,&nbsp;Jing Hou,&nbsp;Heng-Lin Cui","doi":"10.1007/s00792-022-01280-1","DOIUrl":"https://doi.org/10.1007/s00792-022-01280-1","url":null,"abstract":"<p><p>Six novel halophilic archaeal strains of XZYJT10^T, XZYJ18^T, XZYJT40^T, XZYJT49^T, YCN54^T and LT46^T were isolated from a solar saltern in Tibet, a salt lake in Shanxi, and a saline soil in Xinjiang, China. Sequence similarities of 16S rRNA and rpoB' genes among strains XZYJT10^T, XZYJ18^T, XZYJT40^T, XZYJT49^T, YCN54^T, LT46^T and current members of Halorussus were 90.6-97.8% and 87.8-96.4%, respectively. The average nucleotide identity and in silico DNA-DNA hybridization values among these six strains and current Halorussus members were in the range of 76.5-87.5% and 21.0-33.8%, respectively. These values were all below the species boundary threshold values. The phylogenomic tree based on 122 conserved archaeal protein marker genes revealed that the six novel strains formed individual distinct branches and clustered tightly with Halorussus members. Several phenotypic characteristics distinguished the six strains from current Halorussus members. Polar lipid analysis showed that the six novel strains contained phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and two to three glycolipids. Phenotypic, chemotaxonomic and phylogenetic properties showed that the six strains represented six novel species within the genus Halorussus, for which the names Halorussus vallis sp. nov., Halorussus aquaticus sp. nov., Halorussus gelatinilyticus sp. nov., Halorussus limi sp. nov., Halorussus salilacus sp. nov., and Halorussus salinisoli sp. nov. are proposed.</p>","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33511058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electron transfer of extremophiles in bioelectrochemical systems.","authors":"Miriam Edel,&nbsp;Laura-Alina Philipp,&nbsp;Jonas Lapp,&nbsp;Johannes Reiner,&nbsp;Johannes Gescher","doi":"10.1007/s00792-022-01279-8","DOIUrl":"https://doi.org/10.1007/s00792-022-01279-8","url":null,"abstract":"<p><p>The interaction of bacteria and archaea with electrodes is a relatively new research field which spans from fundamental to applied research and influences interdisciplinary research in the fields of microbiology, biochemistry, biotechnology as well as process engineering. Although a substantial understanding of electron transfer processes between microbes and anodes and between microbes and cathodes has been achieved in mesophilic organisms, the mechanisms used by microbes under extremophilic conditions are still in the early stages of discovery. Here, we review our current knowledge on the biochemical solutions that evolved for the interaction of extremophilic organisms with electrodes. To this end, the available knowledge on pure cultures of extremophilic microorganisms has been compiled and the study has been extended with the help of bioinformatic analyses on the potential distribution of different electron transfer mechanisms in extremophilic microorganisms.</p>","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9556394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33502458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isotherm and kinetics modeling of biosorption and bioreduction of the Cr(VI) by Brachybacterium paraconglomeratum ER41.","authors":"Kaoutar Harboul, Imad Alouiz, Khalil Hammani, Abdenbi El-Karkouri","doi":"10.1007/s00792-022-01278-9","DOIUrl":"10.1007/s00792-022-01278-9","url":null,"abstract":"<p><p>Chromium is one of the most widely used metals in industry. Hexavalent form [Cr(VI)], which is found in industrial discharges, is very toxic and very soluble in water. From soil taken from an abandoned lead and iron mine, a bacterial strain capable of reducing Cr(VI) was isolated and identified as Brachybacterium paraconglomeratum ER41. Objective of this work was to evaluate the power of this bacterium to reduce Cr(VI). Results obtained showed that this bacterium is capable of eliminating 100 mg/L of Cr(VI) after 48 h (pH 8 and temperature 30 °C). For modeling biosorption kinetics, pseudo-first-order and intraparticle diffusion models gave a better fit. Furthermore, the adsorption mechanism conformed well to Langmuir's isothermal model indicating monolayer type sorption. Biomass analysis of this bacterium before and after contact with chromium by scanning electron microscopy-energy-dispersive X-ray and by Fourier transform infrared spectroscopy showed that the surface ligands of bacterial wall are probably responsible for biosorption and bioreduction process. These results suggest a potential application of B. paraconglomeratum ER41 in bioremediation of polluted discharges.</p>","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33479359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The catalytic and structural basis of archaeal glycerophospholipid biosynthesis.","authors":"Niels A W de Kok,&nbsp;Arnold J M Driessen","doi":"10.1007/s00792-022-01277-w","DOIUrl":"https://doi.org/10.1007/s00792-022-01277-w","url":null,"abstract":"<p><p>Archaeal glycerophospholipids are the main constituents of the cytoplasmic membrane in the archaeal domain of life and fundamentally differ in chemical composition compared to bacterial phospholipids. They consist of isoprenyl chains ether-bonded to glycerol-1-phosphate. In contrast, bacterial glycerophospholipids are composed of fatty acyl chains ester-bonded to glycerol-3-phosphate. This largely domain-distinguishing feature has been termed the \"lipid-divide\". The chemical composition of archaeal membranes contributes to the ability of archaea to survive and thrive in extreme environments. However, ether-bonded glycerophospholipids are not only limited to extremophiles and found also in mesophilic archaea. Resolving the structural basis of glycerophospholipid biosynthesis is a key objective to provide insights in the early evolution of membrane formation and to deepen our understanding of the molecular basis of extremophilicity. Many of the glycerophospholipid enzymes are either integral membrane proteins or membrane-associated, and hence are intrinsically difficult to study structurally. However, in recent years, the crystal structures of several key enzymes have been solved, while unresolved enzymatic steps in the archaeal glycerophospholipid biosynthetic pathway have been clarified providing further insights in the lipid-divide and the evolution of early life.</p>","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40618200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of carnitine in adaptation of Chromohalobacter salexigens DSM 3043 and its mutants to osmotic and temperature stress in defined medium.","authors":"Xiang-Lin Meng,&nbsp;Xia Gao,&nbsp;Yuan-Ming Si,&nbsp;Li-Li Xu,&nbsp;Li-Zhong Guo,&nbsp;Wei-Dong Lu","doi":"10.1007/s00792-022-01276-x","DOIUrl":"https://doi.org/10.1007/s00792-022-01276-x","url":null,"abstract":"<p><p>L-Carnitine is widespread in nature, but little information is available on its metabolism and physiological functions in moderate halophiles. In this study, we found that Chromohalobacter salexigens DSM 3043 could utilize carnitine not only as a nutrient, but also as an osmolyte. When grown at 37 °C under salt-stress conditions, the strain utilized carnitine as an osmoprotectant by enzymatically converting it into GB. When grown at low and high temperature, both carnitine and its metabolic intermediate GB were simultaneously accumulated intracellularly, serving as cryoprotectants and thermoprotectants. The genes (csal_3172, csal_3173, and csal_3174) which were predicted to participate in L-carnitine degradation to GB were deleted to construct the corresponding mutants. The effects of salinity and temperature on the growth rates and cytoplasmic solute pools of the C. salexigens wild-type and mutant strains were investigated. ¹³C-NMR analysis revealed that GB was still detected in the Δcsal_3172Δcsal_3173Δcsal_3174 mutant grown in a defined medium with added DL-carnitine, but not with L-carnitine, indicating that an unidentified D-carnitine degradation pathway exists in C. salexigens. Taken together, the data presented in this study expand our knowledge on carnitine metabolism and its physiological functions in C. salexigens exposed to single or multiple environmental abiotic stress.</p>","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40611225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organic layer characteristics and microbial utilization of the biosulfur globules produced by haloalkaliphilic Thioalkalivibrio versutus D301 during biological desulfurization.","authors":"Zhixia Liu,&nbsp;Maohua Yang,&nbsp;Tingzhen Mu,&nbsp;Jinlong Liu,&nbsp;Linxu Chen,&nbsp;Delu Miao,&nbsp;Jianmin Xing","doi":"10.1007/s00792-022-01274-z","DOIUrl":"https://doi.org/10.1007/s00792-022-01274-z","url":null,"abstract":"<p><p>The haloalkaliphilic genus Thioalkalivibrio, widely used in bio-desulfurization, can oxidize H₂S to S^o, which is excreted outside cells in the form of biosulfur globules. As by-product of bio-desulfurization, information on biosulfur globules is still very scant, which limits its high-value utilization. In this paper, the characteristics of biosulfur globules produced by Thioalkalivibrio versutus D301 and the possibility of cultivating sulfur-oxidizing bacteria as a high biological-activity sulfur source were studied. The sulfur element in the biosulfur globules existed in the form α-S₈, which was similar to chemical sulfur. The biosulfur globule was wrapped with an organic layer composed of polysaccharides and proteins. The composition of this organic layer could change. In the formation stage of biosulfur globules, the organic layer was dominated by polysaccharides, and in later stage, proteins became the main component. We speculated that the organic layer was mainly formed by the passive adsorption of organic matter secreted by cells. The existence of organic layer endowed biosulfur with better bioavailability. Compared with those found using chemical sulfur, the growth rates of Acidithiobacillus thiooxidans ATCC 19377^T, Thiomicrospira microaerophila BDL05 and Thioalkalibacter halophilus BDH06 using biosulfur increased several folds to an order of magnitude, indicating that biosulfur was a good sulfur source for cultivating sulfur-oxidizing bacteria.</p>","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40625661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halorientalis salina sp. nov., Halorientalis marina sp. nov., Halorientalis litorea sp. nov.: three extremely halophilic archaea isolated from a salt lake and coarse sea salt.","authors":"Bei-Bei Wang,&nbsp;Ya-Ping Sun,&nbsp;Zhang-Ping Wu,&nbsp;Xi-Wen Zheng,&nbsp;Jing Hou,&nbsp;Heng-Lin Cui","doi":"10.1007/s00792-022-01275-y","DOIUrl":"https://doi.org/10.1007/s00792-022-01275-y","url":null,"abstract":"<p><p>Three halophilic archaeal strains, NEN8^T, GDY88^T and ZY14^T, were isolated from a salt lake in Tibet and coarse sea salt samples from Guangdong and Hebei, China, respectively. These strains formed three separate clades (showing 94.4-95.8% and 87.1-89.4% similarities, respectively) and then clustered with the current Halorientalis members (showing 90.7-97.6% and 87.0-91.2% similarities, respectively), as revealed by phylogenetic analyses based on 16S rRNA and rpoB' genes. The overall genome-related index, average nucleotide identity (ANI), in silico DNA-DNA hybridization (DDH), average amino acid identity (AAI) and the percentage of conserved proteins (POCP) values, among the three strains and members of the genus Halorientalis were 76.0-88.0%, 21.3-37.2%, 69.0-88.3% and 57.7-78.1%, clearly below the threshold values for species demarcation. Strains NEN8^T, GDY88^T and ZY14^T could be distinguished from current Halorientalis species according to differential phenotypic characteristics. The major polar lipids of the three strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1) and disulfated mannosyl glucosyl diether (S₂-DGD). In addition, mannosyl glucosyl diether (DGD-1) was detected in strain NEN8^T and phosphatidic acid (PA), posssulfated galactosyl mannosyl glucosyl diether (S-TGD-1) and sulfated mannosyl glucosyl diether-phosphatidic acid (S-DGD-PA) were observed in strain ZY14^T. These results revealed that strains NEN8^T (= CGMCC 1.17213^T = JCM 34155^T), GDY88^T (= CGMCC 1.18548^T = JCM 34481^T) and ZY14^T (= CGMCC 1.17178^T = JCM 34154^T) represent three novel species of the genus Halorientalis, for which the names Halorientalis salina sp. nov., Halorientalis marina sp. nov. and Halorientalis litorea sp. nov. are proposed.</p>","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40598033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis of heavy metal-resistant Halobacterium salinarum isolated from Sfax solar saltern sediments.","authors":"Houda Baati,&nbsp;Mariem Siala,&nbsp;Chafai Azri,&nbsp;Emna Ammar,&nbsp;Christopher Dunlap,&nbsp;Mohamed Trigui","doi":"10.1007/s00792-022-01273-0","DOIUrl":"https://doi.org/10.1007/s00792-022-01273-0","url":null,"abstract":"<p><p>The draft genome sequences of five archaeal strains, isolated from Sfax solar saltern sediments and affiliated with Halobacterium salinarum, were analyzed in order to reveal their adaptive strategies to live in hypersaline environments polluted with heavy metals. The genomes of the strains (named AS1, AS2, AS8, AS11, and AS19) are found to contain 2,060,688; 2,467,461; 2,236,624; 2,432,692; and 2,428,727 bp respectively, with a G + C content of 65.5, 66.0, 67.0, and 66.2%. The majority of these genes (43.69-55.65%) are annotated as hypothetical proteins. Growth under osmotic stress is possible by genes coding for potassium uptake, sodium efflux, and kinases, as well as stress proteins, DNA repair systems, and proteasomal components. These strains harbor many genes responsible for metal transport/resistance, such as: copper-translocating P-type ATPases, ABC transporter, and cobalt-zinc-cadmium resistance protein. In addition, detoxification enzymes and secondary metabolites are also identified. The results show strain AS1, as compared to the other strains, is more adapted to heavy metals and may be used in the bioremediation of multi-metal contaminated environments. This study highlights the presence of several commercially valuable bioproducts (carotenoids, retinal proteins, exopolysaccharide, stress proteins, squalene, and siderophores) and enzymes (protease, sulfatase, phosphatase, phosphoesterase, and chitinase) that can be used in many industrial applications.</p>","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40528280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil substrate culturing approaches recover diverse members of Actinomycetota from desert soils of Herring Island, East Antarctica.","authors":"Nicole Benaud,&nbsp;Devan S Chelliah,&nbsp;Sin Yin Wong,&nbsp;Belinda C Ferrari","doi":"10.1007/s00792-022-01271-2","DOIUrl":"https://doi.org/10.1007/s00792-022-01271-2","url":null,"abstract":"<p><p>Antimicrobial resistance is an escalating health crisis requiring urgent action. Most antimicrobials are natural products (NPs) sourced from Actinomycetota, particularly the Streptomyces. Underexplored and extreme environments are predicted to harbour novel microorganisms with the capacity to synthesise unique metabolites. Herring Island is a barren and rocky cold desert in East Antarctica, remote from anthropogenic impact. We aimed to recover rare and cold-adapted NP-producing bacteria, by employing two culturing methods which mimic the natural environment: direct soil culturing and the soil substrate membrane system. First, we analysed 16S rRNA gene amplicon sequencing data from 18 Herring Island soils and selected the soil sample with the highest Actinomycetota relative abundance (78%) for culturing experiments. We isolated 166 strains across three phyla, including novel and rare strains, with 94% of strains belonging to the Actinomycetota. These strains encompassed thirty-five 'species' groups, 18 of which were composed of Streptomyces strains. We screened representative strains for genes which encode polyketide synthases and non-ribosomal peptide synthetases, indicating that 69% have the capacity to synthesise polyketide and non-ribosomal peptide NPs. Fourteen Streptomyces strains displayed antimicrobial activity against selected bacterial and yeast pathogens using an in situ assay. Our results confirm that the cold-adapted bacteria of the harsh East Antarctic deserts are worthy targets in the search for bioactive compounds.</p>","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2022-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9279279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40590174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incomplete denitrification phenotypes in diverse Thermus species from diverse geothermal spring sediments and adjacent soils in southwest China.","authors":"Chrisabelle C Mefferd, Enmin Zhou, Cale O Seymour, Noel A Bernardo, Shreya Srivastava, Amanda J Bengtson, Jian-Yu Jiao, Hailiang Dong, Wen-Jun Li, Brian P Hedlund","doi":"10.1007/s00792-022-01272-1","DOIUrl":"10.1007/s00792-022-01272-1","url":null,"abstract":"<p><p>A few members of the bacterial genus Thermus have been shown to be incomplete denitrifiers, terminating with nitrite (NO₂^-) or nitrous oxide (N₂O). However, the denitrification abilities of the genus as a whole remain poorly characterized. Here, we describe diverse denitrification phenotypes and genotypes of a collection of 24 strains representing ten species, all isolated from a variety of geothermal systems in China. Confirmed terminal products of nitrate reduction were nitrite or N₂O, while nitric oxide (NO) was inferred as the terminal product in some strains. Most strains produced N₂O; complete denitrification was not observed. Denitrification phenotypes were largely consistent with the presence of denitrification genes, and strains of the same species often had the same denitrification phenotypes and largely syntenous denitrification gene clusters. Genes for nirS and nirK coexisted in three Thermus brockianus and three Thermus oshimai genomes, which is a unique hallmark of some denitrifying Thermus strains and may be ecologically important. These results show that incomplete denitrification phenotypes are prominent, but variable, within and between Thermus species. The incomplete denitrification phenotypes described here suggest Thermus species may play important roles in consortial denitrification in high-temperature terrestrial biotopes where sufficient supply of oxidized inorganic nitrogen exists.</p>","PeriodicalId":12302,"journal":{"name":"Extremophiles","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9270275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40481194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}