E. Kariuki, Erika E. Lovo, T. Price, Vrajesh B. Parikh, Emily B. Duren, P. Avery, C. Minteer
{"title":"The Consumption and Survival Rate of Lilioceris cheni (Coleoptera: Chrysomelidae) on Air Potato Leaves Exposed to Cordyceps javanica (Hypocreales: Cordycipitaceae)","authors":"E. Kariuki, Erika E. Lovo, T. Price, Vrajesh B. Parikh, Emily B. Duren, P. Avery, C. Minteer","doi":"10.1653/024.105.0313","DOIUrl":"https://doi.org/10.1653/024.105.0313","url":null,"abstract":"Summary PFR-97 20% WDG (PFR-97) is a broad-spectrum entomopathogenic fungal biopesticide that contains the active ingredient Cordyceps javanica (Frieder. & Bally) Kepler, B. Shrestha & Spatafora (Hypocreales: Cordycipitaceae). This biopesticide is labelled for controlling aphids, psyllids, spider mites, thrips, and whiteflies in various crops, but also can infect certain chrysomelid species. Pest control activities applying PFR-97 in 1 agroecosystem may inadvertently drift and affect biological control agents purposefully released in another ecosystem. Therefore, our goal was to determine if PFR-97 was compatible when applied near Lilioceris cheni Gressitt and Kimoto (Coleoptera: Chrysomelidae), a chrysomelid biological control agent for the invasive air potato. The consumption and survival rates of adult beetles after exposure to PFR-97 were investigated via leaf bioassays. Thirty L. cheni adults were provisioned individually with a Dioscorea bulbifera L. (Dioscoreaceae) leaf sprayed with either a C. javanica suspension at the concentration of 106, 105, 103, or 102 blastospores per mL-1 or sterile distilled water (control) for 72 h. Untreated leaves were provided every 3 d for an additional 18 d and leaf consumption was determined. Results showed the survival and leaf consumption of the L. cheni adults did not differ among treatments and control. Overall, our study demonstrated that any inadvertent exposure of L. cheni adults to C. javanica via spray drift is unlikely to impact the performance of the adult biological control agents. Sumario PFR-97 20% WDG (PFR-97) es un bioplaguicida fungifero entomopa-tógeno de amplio espectro que contiene el ingrediente activo Cordyceps javanica (Frieder. & Bally) Kepler, B. Shrestha & Spatafora (Hypocreales: Cordycipitaceae). Este biopesticida está etiquetado para el control de áfidos, psílidos, araña rojas, trips y mosca blancas en varios cultivos, pero también puede infectar a ciertas especies de crisomélidos. Las actividades de control de plagas que aplican PFR-97 en 1 agroecosistema pueden derivar inadvertidamente y afectar a los agentes de control biológico liberados intencionalmente en otro ecosistema. Por lo tanto, nuestro ob-jetivo fue determinar si PFR-97 era compatible cuando se aplicaba cerca de Lilioceris cheni Gressitt and Kimoto (Coleoptera: Chrysomelidae), un agente de control biológico de crisomélidos para la papa aérea invasora, Dioscorea bulbifera L. (Dioscoreaceae). Se investigaron mediante bioen-sayos en hojas las tasas de consumo y sobrevivencia de escarabajos adultos después de la exposición a PFR-97. Se suministraron treinta adultos de L. cheni individualmente con una hoja de D. bulbifera rociada con una suspensión de C. javanica a una concentración de 106, 105, 103, or 102 blastosporas por mL-1 o agua destilada estéril (control) durante 72 h. Se proporcionaron hojas sin tratar cada 3 días durante 18 días adicionales y se determinó el consumo de hojas. Los resultados mostraron que la so","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48512754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Bai, Zhenqi Tian, Bo Gao, Zhe Liu, Ling Wang, Jian Liu
{"title":"Performance of Aphis glycines Matsumura (Hemiptera: Aphididae) Reared Under Different Methods","authors":"B. Bai, Zhenqi Tian, Bo Gao, Zhe Liu, Ling Wang, Jian Liu","doi":"10.1653/024.105.0306","DOIUrl":"https://doi.org/10.1653/024.105.0306","url":null,"abstract":"Abstract Soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is an important soybean pest in North America. In this study, A. glycines was fed on detached leaves of Glycine max (L.) Merrill (Fabaceae) at 20, 23, 26, 29, and 32 °C under 3 rearing methods: leaf-disc method, filter paper method, and moisturized cotton method. Aphis glycines life parameters, including nymph stage duration, adult lifespan, adult fecundity, adult body size, and intrinsic rate of increase were measured. There were no significant differences in nymph stage duration among these rearing methods at 20 to 32 °C. Within this temperature range, the filter paper method and the moisturized cotton method resulted in similar or greater adult lifespans, adult fecundities, adult body sizes, and intrinsic rates of increase in comparison to the leaf-disc method. When nymphs of A. glycines were reared at 20 to 29 °C, the experimental labor required for the moisturized cotton method was less than that for the filter paper method and the leaf-disc method. Considering the development, reproduction, and experimental labor, the moisturized cotton method is recommended as a good detached leaf method for rearing individual A. glycines. These conclusions will be useful and informational for researchers who study A. glycines, especially when selecting methods to rear A. glycines individually in the laboratory. Resumen El pulgón de la soja, Aphis glycines Matsumura (Hemiptera: Aphididae), es una plaga importante de la soja en América del Norte. En este estudio, A. glycines fue alimentado con hojas desprendidas de Glycine max (L.) Merrill (Fabaceae) a 20, 23, 26, 29 y 32 °C bajo 3 métodos de crianza: método de disco de hoja, método de papel de filtro y método de algodón humedecido. Se midieron los parámetros de vida del A. glycines, incluyendo la duración de la estadio del ninfa, la duración de vida de los adultos, la fecundidad, el tamaño corporal adulto y la tasa intrínseca de aumento. No hubo diferencias significativas en la duración del estadio de ninfa entre estos métodos de crianza a 20 a 32 °C. Dentro de este rango de temperatura, el método del papel de filtro y el método del algodón humedecido dieron resultados similares o mayores sobre la duración de vida de los adultos, la fecundidad, el tamaño corporal de adultos y la tasa intrínseca de aumento en comparación con el método del disco de hoja. Cuando se criaron las ninfas a una temperatura de 20 a 29 °C, el trabajo experimental requerido para el método del algodón humedecido fue menor que los métodos del papel de filtro y del disco de hoja. Teniendo en cuenta el desarrollo, la reproducción y el trabajo experimental, se recomienda el método del algodón humedecido como un buen método de hojas sueltas para criar los individuales de A. glycines. Estas conclusiones serán útiles e informativas para los investigadores que estudian A. glycines, especialmente al seleccionar métodos para criar A. glycines individualmente en el laborato","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45284691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Response of Wasmannia auropunctata (Hymenoptera: Formicidae) to Water-Soaked Imported Fire Ant Baits","authors":"D. Oi, A. Lucky, Dina M. Liebowitz","doi":"10.1653/024.105.0202","DOIUrl":"https://doi.org/10.1653/024.105.0202","url":null,"abstract":"Abstract The little fire ant, Wasmannia auropunctata (Roger) (Hymenoptera: Formicidae), is a stinging invasive ant that can establish extremely large, dominating populations in tropical and subtropical regions. In Florida, it is well established in south and central Florida with a reported northern limit of Marion County, Florida, USA. However, in 2018 to 2019 overwintering populations were discovered farther north in Gainesville, Florida, USA. There is a need to develop effective management options suitable for the site uses of these recently discovered infestations. Most commercial imported fire ant baits are formulated on a corn grit carrier that, when exposed to moisture, is thought to compromise bait effectiveness. Due to the humid and rainy summer weather in this region, the objective of this study was to determine the acceptance and efficacy of water-soaked ant baits on W. auropunctata, some of which had purported moisture resistance. Bait acceptance tests conducted in the field with dry baits determined that baits containing the active ingredients spinosad and pyriproxyfen were accepted poorly, while W. auropunctata accepted both dry and wet baits containing hydramethylnon, metaflumizone, indoxacarb, and abamectin. Laboratory colonies given access to either dry or wet baits exhibited significant declines in workers, brood, and queens with several colonies being eliminated. The results of this study indicated that water-soaked imported fire ant baits could control W. auropunctata, and moisture exposure did not cause baits to become unpalatable. However, these results should be validated under field conditions, where precipitation may reduce the accessibility of baits to foraging ants. Resumen La pequeña hormiga de fuego, Wasmannia auropunctata (Roger) (Hymenoptera: Formicidae), es una hormiga invasora urticante que puede establecer poblaciones dominantes extremadamente grandes en regiones tropicales y subtropicales. En Florida, está bien establecida en el sur y el centro de la Florida con un límite norte hasta el condado de Marion, Florida, EE. UU. Sin embargo, entre el 2018 y 2019 se descubrieron poblaciones que hibernan más al norte, en Gainesville, Florida. Existe la necesidad de desarrollar opciones de manejo efectivas adecuadas para los usos del sitio de estas infestaciones descubiertas recientemente. La mayoría de los cebos comerciales importados para hormigas de fuego están formulados con grano de maíz como portador que cuando se expone a la humedad, se cree que compromete la eficacia del cebo. Debido al clima húmedo y lluvioso del verano en esta región, el objetivo de este estudio fue determinar la aceptación y eficacia de los cebos para hormigas empapados en agua sobre W. auropunctata, algunos de los cuales tenían supuesta resistencia a la humedad. Las pruebas de aceptación de cebos realizadas en el campo con cebos secos determinaron que los cebos que contenían los ingredientes activos spinosad y pyriproxyfen fueron mal aceptados,","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47079835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. A. Wagan, Wenjun Wang, H. Hua, Lyu Rong-Hua, W. Cai
{"title":"The Effects of Three Essential Oils on Adult Repellency, Larval Fumigant Toxicity, and Egg Hatch of Tribolium castaneum (Coleoptera: Tenebrionidae)","authors":"T. A. Wagan, Wenjun Wang, H. Hua, Lyu Rong-Hua, W. Cai","doi":"10.1653/024.105.0209","DOIUrl":"https://doi.org/10.1653/024.105.0209","url":null,"abstract":"Abstract The essential oils of Cedrus atlantica (Endl.) (Pinales: Pinaceae), Mentha x piperita L. (Lamiales: Lamiaceae), and Santalum paniculatum L. (Santalales: Santalaceae), were evaluated for their bioactivity on red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). The most pharmacologically effective oil for this beetle species was that of M. piperita followed by C. atlantica and S. paniculatum. Mentha piperita provided about 74% repellency (at 1 d) to adults, whereas larval fumigant mortality was nearly 50% at 8 h of exposure. The greatest apparent reduction of egg hatch occurred with S. paniculatum (76%) when compared with M. piperita (25%) after 15 d. However, we believe that all 3 essential oils could be helpful for controlling red flour beetles to some degree. After recording bioactivity for all essential oils tested, each was subjected to gas chromatography-mass spectrometry (GC-MS) analysis to identify its chemical components. Further studies should be conducted to test the bioactivity of the individual chemical components of these oils for T. castaneum. Resumen Los aceites esenciales de Cedrus atlantica (Endl.) (Pinales: Pinaceae), Mentha x piperita L. (Lamiales: Lamiaceae) y Santalum paniculatum L. (Santalales: Santalaceae) fueron evaluados por su bioactividad sobre el escarabajo rojo de la harina, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). El aceite más farmacológicamente eficaz para esta especie de escarabajo fue el de M. piperita seguido de C. atlantica y S. paniculatum. Mentha piperita proporcionó aproximadamente 74% de repelencia (a 1 dia) a los adultos, mientras que la mortalidad de larvales expuestas a los fumigantes fue de casi el 50% a las 8 horas de exposición. La mayor reducción de huevos sucedio con S. paniculatum (76%) en comparación con M. piperita (25%) después de 15 días. Sin embargo, creemos que los 3 aceites esenciales podrían ser útiles para controlar los escarabajos rojos de la harina hasta cierto punto. Después de registrar la bioactividad de todos los aceites esenciales analizados, cada uno de ellos fue sometido a un análisis de cromatografía de gases y espectrometría de masas (GC-MS) para identificar sus componentes químicos. Se deben realizar más estudios para probar la bioactividad de los componentes químicos individuales de estos aceites para T. castaneum.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42123137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Toxicity, Repellency, and Laboratory Performance of Consumer Bait Products for German Cockroach (Blattodea: Ectobiidae) Management","authors":"A. Appel, B. Dingha, Marla J. Eva, L. Jackai","doi":"10.1653/024.105.0203","DOIUrl":"https://doi.org/10.1653/024.105.0203","url":null,"abstract":"Abstract Toxicity, repellency, and laboratory performance of consumer bait formulations were evaluated for control of 7 insecticide-resistant, field-collected strains of the German cockroach, Blattella germanica (L.) (Blattodea: Ectobiidae) in continuous exposure and Ebeling choice box assays. Solid and gel baits contained avermectin, dinotefuran, fipronil, or hydramethylnon as active ingredients, and were active for control of all German cockroach strains. However, the laboratory susceptible strain was generally more sensitive (lower median lethal times or LT50 values) than the field-collected strains. Resistance ratios in continuous exposure tests ranged from 0.37 for 0.01% fipronil (strain H) to 14.23 for 0.05% dinotefuran (strain DCC2). The LT50 values for most baits and strains generally were greater when tested in Ebeling choice boxes compared with continuous exposure tests. Resistance ratios ranged as high as 98.43 for 0.05% dinotefuran for control of strain DCC2. All strains had resistance to 0.05% dinotefuran bait, and the resistance ratios were greatest when tested in Ebeling choice boxes. All bait formulations had some repellency to most strains; however, repellency was never greater than 70% for any treatment–strain combination. Combining Ebeling choice box mortality and repellency data, maximum estimated performance index values (PIMax) reached 100, i.e., no repellency and complete mortality, for most treatments. There was not complete mortality of the majority of strains exposed to 0.05% dinotefuran bait and therefore the PIMax value did not reach 100. The rate of increase in performance index value over time or tPImax/2 ranged 0.26 and 7.85 d. Our results indicate that although all baits were toxic to multi-resistant strains of German cockroaches, there was significant resistance or tolerance to many formulations that would likely negatively impact field control. Resumen Se evaluó la toxicidad, repelencia y desempeño en el laboratorio de formulaciones de cebo de consumo para controlar 7 cepas resistentes a insecticidas de la cucaracha alemana, Blattella germanica (L.) (Blattodea: Ectobiidae) y recolectadas en el campo, en exposición continua y ensayos de cuadro de selección de Ebeling. Los cebos sólidos y en gel contenían avermectina, dinotefurano, fipronil o hidrametilnon como ingredientes activos y fueron activos para el control de todas las cepas de cucarachas alemanas. Sin embargo, la cepa susceptible de laboratorio fue generalmente más sensible (tiempos letales medianos o valores TL50 más bajos) que las cepas recolectadas en el campo. Los índices de resistencia en las pruebas de exposición continua oscilaron entre 0,37 para fipronil al 0,01 % (cepa H) y 14,23 para dinotefurano al 0,05 % (cepa DCC2). Los valores LT50 para la mayoría de los cebos y cepas generalmente fueron mayores cuando se probaron en cajas de elección de Ebeling en comparación con las pruebas de exposición continua. Las proporciones de resistencia oscilaron h","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41302838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maythe Morales-Gálvez, Renato Villegas-Luján, R. Plowes, L. Gilbert, Tanner A. Matson, G. Gallegos-Morales, S. Sanchez-Peña
{"title":"Natural Egg Parasitism by Scelionidae on a Phycitine Cactus Moth in Mexico","authors":"Maythe Morales-Gálvez, Renato Villegas-Luján, R. Plowes, L. Gilbert, Tanner A. Matson, G. Gallegos-Morales, S. Sanchez-Peña","doi":"10.1653/024.105.0212","DOIUrl":"https://doi.org/10.1653/024.105.0212","url":null,"abstract":"Summary The detection of native natural enemies that can use Cactoblastis cactorum as a host is relevant after its invasion of North America. We detected wasps (Scelionidae) parasitizing eggs of Melitara cf. junctolineella (a closely related cactus moth in the Phycitini) in Bustamante, Nuevo Leon, northeastern Mexico. This is the first report of Scelionidae parasitizing eggs of phycitine cactus moths. The capability of this wasp to parasitize C. cactorum eggs should be investigated. Sumario La detección de enemigos naturales nativos que pueden utilizar a Cactoblastis cactorum como hospedero, es relevante tras su invasión a Norteamérica. Detectamos avispas (Scelionidae) parasitando huevos de Melitara cf. junctolineella (una polilla del cactus en Phycitini estrechamente relacionada con C. cactorum) en Bustamante, Nuevo Leon, noreste de México. Este es el primer reporte de Scelionidae parasitando huevos de polillas del cactus (Phycitini). Debe investigarse la capaci-dad de esta avispa para parasitar los huevos de C. cactorum.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47161841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Camille Carey, W. Hoback, J. S. Armstrong, A. Zarrabi
{"title":"The Effects of Light-Emitting Diode and Conventional Lighting on Sorghum Physiology and Sugarcane Aphid Interaction","authors":"Camille Carey, W. Hoback, J. S. Armstrong, A. Zarrabi","doi":"10.1653/024.105.0207","DOIUrl":"https://doi.org/10.1653/024.105.0207","url":null,"abstract":"Abstract Light-emitting diodes often are used to substitute and enhance fluorescent or incandescent light for plants that are grown in climate-controlled environments. These lights often are chosen over other light sources because of the light-emitting diodes' durability, long life, enhanced wavelength for specific plant species, lower energy costs, lower surface heat safety risk, and easier ability to incorporate into advanced climate control systems. However, previous studies have shown that characteristics of Sorghum bicolor (L.) Moench (Poaceae) are altered under light-emitting diodes. Therefore, we grew 4 cultivars of sorghum in growth chambers with either conventional or light-emitting diodes. Plants were infested with either the sugarcane aphid, Melanaphis sacchari (Zehntner) (Hemiptera: Aphididae) or were un-infested (control). Sorghum grown under light-emitting diodes was shorter and produced more leaves that were wider than those of plants grown under conventional lights. Two of the cultivars had lower photosynthetic rates and reduced stomatal conductance under light-emitting diodes. When exposed to sugarcane aphids, resistant cultivars tolerated aphid feeding with reduced damage under conventional lights but were similar to susceptible cultivars when grown under light-emitting diodes. Our results suggest that light-emitting diodes affect sorghum physiology and morphology, and also compromises resistance to herbivores. Our study provides further evidence that the physiological effects of different light spectra and interaction of plant defenses and herbivores need to be tested across a broad range of plant groups. Resumen Los diodos emisores de luz a menudo se utilizan para sustituir y mejorar la luz fluorescente o incandescente para las plantas que se cultivan en entornos con clima controlado. Estas luces a menudo se eligen en lugar de otras fuentes de luz debido a la durabilidad de los diodos emisores de luz, su larga vida útil, su longitud de onda mejorada para especies específicas de plantas, sus costos de energía más bajos, su riesgo de seguridad contra el calor superficial más bajo y su capacidad más fácil de incorporarse a sistemas avanzados de control climático. Sin embargo, estudios previos han demostrado que las características de Sorghum bicolor (L.) Moench (Poaceae) se alteran bajo luces de diodos emisores de luz. Por lo tanto, cultivamos 4 cultivares de sorgo en cámaras de crecimiento con luces convencionales o con diodos emisores de luz. Las plantas estaban infestadas con el pulgón de la caña de azúcar, Melanaphis sacchari (Zehntner) (Hemiptera: Aphididae) o no estaban infestadas (control). El sorgo cultivado bajo luces de diodos emisores de luz era más corto y producía más hojas que eran más anchas que las de las plantas cultivadas bajo luces convencionales. Dos de los cultivares tenían tasas fotosintéticas más bajas y conductancia estomática reducida bajo luces de diodos emisores de luz. Cuando se expusieron a los áfidos","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46880555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. E. Price, C. Raffin, Seung-Hwan Yun, Katerina Velasco-Graham, Man‐Yeon Choi
{"title":"A Sustainable Mass Rearing Method for Western Flower Thrips, Frankliniella occidentalis (Thysanoptera: Thripidae)","authors":"B. E. Price, C. Raffin, Seung-Hwan Yun, Katerina Velasco-Graham, Man‐Yeon Choi","doi":"10.1653/024.105.0211","DOIUrl":"https://doi.org/10.1653/024.105.0211","url":null,"abstract":"Summary Here we present a simple, cost-effective, and sustainable mass rearing system for western flower thrips, Frankliniella occidentalis, with details of molecular identification. Our rearing methods are improved from other systems used previously because we have organized maintenance responsibilities that occur during a Monday through Friday work wk, and have streamlined environmental and nourishment conditions that have greatly sustained F. occidentallis colonies that we use for various physiological experiments. Sumario Aquí presentamos un sistema de crianza masiva que es sustentable, simple y de costo efectivo para el trips de California, Frankliniella occidentalis, con detalles sobre identificación molecular. Nuestros métodos de crianza son una mejoría en comparación a otros usados previamente ya que hemos organizado responsabilidades de mantenimiento estructuradas alrededor de una semana laboral de lunes a viernes, así como la simplificación de las condiciones ambientales y de alimentación que han sustentado enormemente las colonias de F. occidentallis que usamos para varios experimentos fisiológicos.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48414829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rocío Cruz-Díaz, N. Robledo, H. Reyes-Prado, Daniel Tapia-Marur, V. Castrejón-Gómez
{"title":"Spodoptera frugiperda (Lepidoptera: Noctuidae) Females Can Detect the Sex Pheromone Emitted by Conspecific Females","authors":"Rocío Cruz-Díaz, N. Robledo, H. Reyes-Prado, Daniel Tapia-Marur, V. Castrejón-Gómez","doi":"10.1653/024.105.0204","DOIUrl":"https://doi.org/10.1653/024.105.0204","url":null,"abstract":"Abstract The sex pheromones emitted by Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) females attract males for copulation, but no studies to date have investigated if conspecific females also are attracted. Here, the attraction of females of S. frugiperda to their sex pheromone in flight tunnel laboratory bioassays and field trapping is reported. Genitalia of females and males captured in the field were dissected for taxonomic identification and studied with an environmental scanning electron microscope to know the mating status of the females. In wind tunnel attraction bioassays, virgin females flew upwind and landed on the stimulus, likewise the males, whereas mated females, although they headed for the stimulus, showed fewer landings. The sex ratio of captured insects in the field was 1 female to 4 males. The presence of spermatophores allowed the separation of mated and virgin females using the genitalia; both were found in the traps throughout the sampling period. This study demonstrated that S. frugiperda females autodetect their sex pheromone, and its implications on the management strategy for these moths are discussed. Resumen Las feromonas sexuales emitidas por las hembras de Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) atraen a los machos para la cópula, pero no hay estudios que investiguen si las hembras conespecíficas son atraídas. En este estudio, se informa de la atracción de las hembras de S. frugiperda a su feromona sexual en bioensayos de laboratorio en túnel de vuelo, y trampas de campo. Se disectaron las genitalias de las hembras y los machos capturados en el campo para su identificación taxonómica y también se estudiaron con el microscopio electrónico de barrido ambiental para conocer el estado de apareamiento de las hembras. En los bioensayos de atracción en el túnel de viento, las hembras vírgenes volaron contra el viento y se posaron en el estímulo, al igual que los machos, mientras que las hembras apareadas, aunque se dirigieron al estímulo, mostraron menos aterrizajes. En el campo, la proporción sexual de los insectos capturados fue de una hembra por cada cuatro machos. Las genitalias de las hembras apareadas y vírgenes se distinguieron por la presencia del espermatóforo. Este estudio demuestra que las hembras de S. frugiperda autodetectan su feromona sexual y se discuten sus implicaciones en la estrategia de manejo de estas palomillas.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49587808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}