Florida Entomologist最新文献

{"title":"A Portable Chamber for Experimental Observations of Bactericera Cockerelli on Plant Seedlings and Leaves","authors":"Carolina Delgado-Luna, Álvaro Romero-Castillo, E. Cerna-Chávez, S. Sanchez-Peña","doi":"10.1653/024.105.0412","DOIUrl":"https://doi.org/10.1653/024.105.0412","url":null,"abstract":"Summary We describe a portable, economical plastic chamber for observations on development and reproduction of potato-tomato psyllid, Bactericera cockerelli for up to a few wk on plant material under controlled conditions. It can be used for other insects as well. Sumario Se describe una cámara de plástico portátil y económica para observaciones durante semanas del desarrollo y reproducción de el psílido de la papa y tomate, Bactericera cockerelli en material vegetal bajo condiciones controladas. Esta cámara también se puede utilizar para otros insectos.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47702778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Report of the Exotic Species Megalurothrips usitatus (Thysanoptera: Thripidae) Infesting Three Commercial Legumes in Nayarit, Mexico","authors":"O.J. Cambero Campos, Agustín Cambero Monroy, Jesús Alexander Rodríguez Arrieta, A. Bermúdez, Braulio Alberto Lemus Soriano, C. R. Velasco, Ángel Ignacio Zamora Landa, Mario Orlando Estrada Virgen","doi":"10.1653/024.105.0409","DOIUrl":"https://doi.org/10.1653/024.105.0409","url":null,"abstract":"Summary The bean flower thrips, Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) is native from Asia and is a well-known pest of legumes such as common bean (Phaseolus vulgaris L.), cowpea (Vigna unguiculata [L.] Walp.), pea (Pisum sativum L.), and lima bean (Phaseolus limensis Macf.) (all Fabaceae). This thrips species has been recorded recently in the Americas (USA and Cuba). Before this study there were no records of M. usitatus in Mexico; this work is the first report of M. usitatus in Mexico. We found this species attacking common beans, jicama, and cowpeas in Nayarit State. In cowpeas, it reduced production by at least 60%. The highest population was observed in jicama but no evidence of severe damage or yield loss was documented; however, the potential damage on the seed production should be evaluated. Authorities, researchers, extension agents, and growers should work together to prevent the dispersal of this pest, which may infest other economically important legumes. Chances of controlling or eradicating M. usitatus in the region are minimal if no effective alternatives are available in the short term. Sumario El trips de la flor del frijol, Megalurothrips usitatus (Bagnall) (Thysanoptera: Thripidae) es originario de Asia y es una conocida plaga de leguminosas como el frijol común (Phaseolus vulgaris L.), el caupí (Vigna unguiculata [L.] Walp.), guisante (Pisum sativum L.) y frijol lima (Phaseolus limensis Macf.) (todas Fabaceae). Esta especie de trips ha sido registrada recientemente en las Américas (EE.UU. y Cuba). Antes de este estudio no había registros de M. usitatus en México; este trabajo es el primer reporte de M. usitatus en México. Encontramos esta especie atacando frijol común, jícama y caupí en el estado de Nayarit. En caupí, redujo la producción en al menos un 60%. La población más alta se observó en jícama pero no se documentó evidencia de daño severo o pérdida de rendimiento; sin embargo, se debe evaluar el daño potencial sobre la producción de semillas. Las autoridades, investigadores, extensionistas y productores deben trabajar juntos para evitar la dispersión de esta plaga, que puede infestar otras leguminosas económicamente importantes. Las posibilidades de controlar o erradicar M. usitatus en la región son mínimas si no se dispone de alternativas eficaces a corto plazo.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42017661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Book Review","authors":"Shiala M. Naranjo, R. Mallinger","doi":"10.1653/024.105.0413","DOIUrl":"https://doi.org/10.1653/024.105.0413","url":null,"abstract":"","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47553620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Hellula phidilealis (Lepidoptera: Pyralidae) Larval Instars by Application of the Dyar Rule","authors":"Omar Hernández-Romero, Néstor Bautista-Martínez;, Lauro Soto-Rojas, J. Romero-Nápoles, Clemente de Jesús García–Ávila","doi":"10.1653/024.105.0408","DOIUrl":"https://doi.org/10.1653/024.105.0408","url":null,"abstract":"Summary Hellula phidilealis (Walker) (Lepidoptera: Pyralidae) is a species associated with cultivation of crucifers. The damage it produces depends on the phenological stage of the plants, its population density, and the instar in which it is found. For this reason, it is important to identify the instars of the species to contribute to defining management tactics focused on its control in hosts of economic importance. The Dyar rule establishes that the width of the cephalic capsule of the larvae increases at a regular geometric progression. The objective of this study was to determine the rates of increase in the size of the cephalic capsules to determine the number of larval instars of H. phidilealis. Because it is a species that has recently acquired importance in Mexico, it is necessary to conduct basic research to determine its biology to enable implementation of appropriate management tactics for each instar. To obtain the images, a Carl Zeiss® (SteREO Discovery V.20) microscope equipped with a Canon® (EOS 50D) camera was used, and JPEG images were captured for later measurement with the program Image J (v. 1.53e). The larval instars of H. phidilealis were adjusted to the Dyar rule (R2 = 0.9639) with a change rate of 0.50, 0.58, 0.92, and 0.54 for instars L2, L3, L4, and L5, respectively; the average of the rates was 0.63. Sumario Hellula phidilealis (Walker) (Lepidoptera: Pyralidae) es una especie asociada con el cultivo de crucíferas, el daño que produce depende de la etapa fenológica de las plantas, la densidad poblacional y del ínstar en el que se encuentre. Por lo anterior, es de importancia identificar los ínstares de esta especie, lo que coadyuvará a definir las tácticas de manejo enfocadas para su control en hospedantes de importancia económica. La Ley de Dyar establece que la anchura de las cápsulas cefálica de las larvas, se incrementa de acuerdo con una progresión geométrica regular. El objetivo de este trabajo fue determinar las tasas de incremento en el tamaño de las cápsulas cefálicas para determinar el número de ínstares larvales de H. phidilealis. Al ser una especie que recientemente ha adquirido importancia en México, es necesario desarrollar investigación básica que permita conocer la biología de la plaga para implementar las tácticas de manejo pertinentes para cada ínstar. Para la obtención de imágenes, se utilizó un microscopio Carl Zeiss ® (SteREO Discovery V.20) acondicionado con una cámara Canon® (EOS 50D); se capturaron imágenes JPEG de cada espécimen para su posterior medición con el programa Image J (v. 1.53e). Los ínstares larvales de H. phidilealis se ajustaron a la Regla de Dyar (R2 = 0.9639) con una tasa de cambio de 0.50, 0.58, 0.92, y 0.54 para los ínstares L2, L3, L4, y L5, respectivamente, el promedio de las tasas fue de 0.63.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41646243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Telenomus alecto (Crawford) (Hymenoptera: Scelionidae), Parasitoid of Diatraea magnifactella Dyar (Lepidoptera: Crambidae) from Jalisco, Mexico: A Study Based on Morphological and Molecular Evidence","authors":"M. Ramírez-Ahuja, M. A. Gómez-Govea, G. Trujillo-Rodríguez, E. Garza-González, I. Rodríguez-Sánchez, E. Talamas","doi":"10.1653/024.105.0407","DOIUrl":"https://doi.org/10.1653/024.105.0407","url":null,"abstract":"Abstract The parasitoid wasp, Telenomus alecto (Crawford) (Hymenoptera: Scelionidae), is reported for the first time parasitizing Diatraea magnifactella Dyar (Lepidoptera: Crambidae) in Jalisco, Mexico. The occurrence of Te. alecto was discovered in May 2017 during a survey of egg parasitoids of D. magnifactella. The field survey was conducted on Saccharum officinarum L. (Poaceae) in Etzatlan, Jalisco, Mexico. In total, 656 eggs were collected, of which 401 were parasitized. The identity of the parasitoids was determined on the basis of morphological evidence and we here provide the barcoding region (COI). Resumen El parasitoide Telenomus alecto (Crawford) (Hymenoptera: Scelionidae) es reportado por primera vez parasitando Diatraea magnifactella Dyar (Lepidoptera: Crambidae) en Jalisco, México. Telenomus alecto fue descubierto en mayo de 2017 durante un estudio sobre los parasitoides de huevo de D. magnifactella. El estudio de campo se realizó en Etzatlan, Jalisco, México sobre Saccharum officinarum L. (Poaceae). En total, se recolectaron 656 huevos, de los cuales 401 estuvieron parasitados. La identidad de los parasitoides se determinó por morfología y mediante la región del código de barras (COI).","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44531777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of Delia platura (Meigen) (Diptera: Anthomyiidae) in Onion and Scallion Crops in Mexico","authors":"M. A. Tejeda-Reyes, Alejandro Guillermo Rojas-Rosales, C. Illescas-Riquelme, A. Hernández-Juárez","doi":"10.1653/024.105.0410","DOIUrl":"https://doi.org/10.1653/024.105.0410","url":null,"abstract":"Summary This study reports the incidence of the seedcorn maggot, or bean seed fly, Delia platura (Meigen) (Diptera: Anthomyiidae), as a primary pest in commercial onion and scallion plantations in Puebla, Mexico. The larvae feed on the bulbs and the basal part of leaves. We found incidences of 5 to 52% of plants in commercial fields damaged by larvae. Sumario En el presente estudio se reporta la incidencia del gusano de la semilla del maíz, o mosca de la semilla del frijol, Delia platura (Meigen) (Diptera: Anthomyiidae), como plaga primaria en plantaciones. Las larvas se alimentan de los bulbos y de la parte basal de las hojas. Se encontraron incidencias del 5 al 52% de plantas dañadas por larvas en plantaciones comerciales.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43351687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Larval Diet on the Flight Capability of the Adult Moth Athetis lepigone (Möschler) (Lepidoptera: Noctuidae)","authors":"Jianrong Huang, Lina Gao, Tessa Cobb, Guoping Li, Caihong Tian, Aiju Duan, H. Feng","doi":"10.1653/024.105.0404","DOIUrl":"https://doi.org/10.1653/024.105.0404","url":null,"abstract":"Abstract Athetis lepigone (Möschler) (Lepidoptera: Noctuidae) is a polyphagous insect which has caused severe damage to corn seedlings in recent yr in the summer corn region of China. It is suggested that this is a migrant species, which annually produces 4 different host-fed generations, but the discovery of its flight performance on each host has been elusive. The effects of 4 host plants—corn, peanut, soybean, and wheat—on the survival ratio of larvae and on the flight performance of A. lepigone moths were investigated under laboratory conditions. The results indicate that wheat was the comparatively preferred diet for larvae. The flight distance of A. lepigone moths increased from 1 d old to a peak level at 3 d and then declined after 4 d old within a 24 h limited flight time. The positive larval-nutrition relationship in terms of flight capability of A. lepigone moths also resulted in the furthest distance and longest duration from the preferred wheat. The maximum distance covered by a single individual fed on wheat for 24 h was 44.55 km. The max duration of an individual was 21.46 h. However, A. lepigone moths had a comparatively higher maximum immediate speed (max speed) when larvae fed on non-preferred host plants such as corn and peanut. The sex of the moths from different hosts had no effect on flight distance, duration, or max speed. There were 2 peaks in flight duration of all 3 to 4 d old tested moths, which meant 2 groups: short flight and long flight duration. The difference between hosts in terms of distance, duration, and max speed as previously mentioned occurred only in the long flight duration group. All these results enhance understanding of the seasonal population dynamics of this migratory moth in the agricultural ecosystem in China. They also have important implications for natural environments in terms of migration and outbreaks. Resumen Athetis lepigone (Möschler) (Lepidoptera: Noctuidae) es un insecto polífago que ha causado graves daños a las plántulas de maíz en los últimos años en la región de maíz de verano de China. Se sugiere que esta es una especie migratoria, que anualmente produce 4 generaciones alimentadas por diferentes hospederos, pero ha eludido el descubrir el desempeño de vuelo en cada hospedero. Los efectos de 4 plantas hospederas—maíz, maní, soja, y trigo—en la tasa de sobrevivencia de las larvas y en el rendimiento de vuelo de las polillas. De A. lepigone fue investigado en condiciones de laboratorio. Los resultados indican que el trigo fue la dieta comparativamente preferida para las larvas. La distancia de vuelo de las polillas A. lepigone aumentó desde el primer día hasta un nivel máximo a los 3 días y luego disminuyó después de los 4 días dentro de un tiempo de vuelo limitado de 24 horas. La relación positiva larva-nutrición en términos de capacidad de vuelo de las polillas A. lepigone también resultó en la mayor distancia y mayor duración del trigo preferido. La distancia máxima recorrida por un s","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41612125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated Size Measurements of Halyomorpha halys (Stål) (Heteroptera: Pentatomidae) with Simple Image-Based Methodology","authors":"A. Tabb, Johanna E. Elsensohn, T. Leskey","doi":"10.1653/024.105.0314","DOIUrl":"https://doi.org/10.1653/024.105.0314","url":null,"abstract":"Summary Measurements of insect external morphology are important to understanding species ecology, biology, and systematics. Precise measurement of width and length can be laborious and time-consuming. Here, we explored an automated approach to insect measurement using an image-based method with adult Halyomorpha halys (Stål) (Heteroptera: Pentatomidae) as a model. Wild-collected H. halys length and width were measured from their ventral side manually with calipers and compared with automated measurements derived from smartphone images. The comparison between manual methods and our automated approach yielded R2 values of 0.69 and 0.84 for width and length, respectively, indicating that an image-based measurement method has potential for usage in future entomological studies. Automated sizing using this image-based method has the potential to reduce the time and costs of performing insect morphological measurements. Sumario Las mediciones de la morfología externa de los insectos son importantes para comprender la ecología, la biología y la sistemática de las especies. La medición precisa de ancho y largo puede ser laboriosa y llevar mucho tiempo. Aquí, exploramos un enfoque automatizado para la medición de insectos utilizando un método basado en imágenes con adultos de Halyomorpha halys (Stål) (Heteroptera: Pentatomidae) co-mo modelo. Se midieron la longitud y el ancho de H. halys recolectados en la naturaleza desde su lado ventral manualmente con calibradores y se compararon con mediciones automatizadas derivadas de imágenes de teléfonos celulares inteligentes. La comparación entre los métodos manuales y nuestro enfoque automatizado arrojó valores R2 de 0,69 y 0,84 para el ancho y el largo, respectivamente, lo que indica que un método de medición basado en imágenes tiene potencial para su uso en futuros estudios entomológicos. El sistema automatizado de determinar dimensiones basado en imágenes tiene el potencial de reducir el tiempo y los costos de realizar mediciones morfológicas de insectos.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44468678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tick (Acarina: Ixodidae) Species and Life Stages Collected from Arkansas Wild Pigs","authors":"Haylee Campbell, K. Loftin, Allen L. Szalanski, D. Steinkraus, Elizabeth Smith","doi":"10.1653/024.105.0303","DOIUrl":"https://doi.org/10.1653/024.105.0303","url":null,"abstract":"Abstract Wild pigs (Sus scrofa L.; Artiodactyla: Suidae) are found in every Arkansas county and are spreading throughout the southeastern USA. This is a public and animal health concern because wild pigs occupy the same geographical areas as humans and livestock, and can harbor over 45 animal pathogens and parasites. Little research has been conducted on the tick species parasitizing Arkansas wild pigs. Tick collections for this study were taken from feral S. scrofa trapped for control purposes by Arkansas Game and Fish Commission, USDA APHIS Wildlife Services, and University of Arkansas Cooperative Extension Service personnel. Between Feb 2019 and Jan 2020, over 3,110 ticks were collected from 220 hogs from 11 Arkansas counties. Four tick species (Acarina: Ixodidae) were collected: Amblyomma americanum (L.) (95.4%; n = 2,966), Dermacentor variabilis (Say) (2.4%; n = 76), Amblyomma maculatum (Koch) (0.6%; n = 18), and Ixodes scapularis (L.) (0.3%; n = 9). Amblyomma americanum was collected from Jan to Nov 2019, and was the only species found in immature life stages. Dermacentor variabilis was collected from Apr 2019 to Oct 2019. Amblyomma maculatum was collected at 1 site in May 2019, and I. scapularis was collected at 1 site in Nov 2019. Resumen Se encuentran los cerdos salvajes (Sus scrofa L.; Artiodactyla: Suidae) en todos los condados de Arkansas y se están extendiendo por todo el sureste de los Estados Unidos. Este es un problema de salud pública y animal porque los cerdos salvajes ocupan las mismas áreas geográficas que los humanos y el ganado, y pueden albergar más de 45 patógenos y parásitos de animales. Se han realizado pocas investigaciones sobre las especies de garrapatas que parasitan a los cerdos salvajes de Arkansas. Las colecciones de garrapatas para este estudio fueron tomadas de S. scrofa salvajes atrapadas con fines de control por parte de la Comisión de Pesca y Caza de Arkansas, los Servicios de Vida Silvestre del USDA/APHIS y el personal del Servicio de Extensión Cooperativa de la Universidad de Arkansas. Más de 3.110 garrapatas de 220 cerdos de 11 condados de Arkansas fueron recolectadas entre febrero del 2019 y enero del 2020. Se recolectaron cuatro especies de garrapatas (Acarina: Ixodidae): Amblyomma americanum (L.) (95,4 %; n = 2.966), Dermacentor variabilis (Say) (2,4 %; n = 76), Amblyomma maculatum (Koch) (0,6 %; n = 18), e Ixodes scapularis (L.) (0,3%; n = 9). Se recolectó A. americanum desde enero a noviembre del 2019 y fue la única especie que se encontró los estadios inmaduros. Se recolectó A. maculatum en sitio 1 en mayo del 2019 e I. scapularis se recolectó en sitio 1 en noviembre del 2019.","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42680264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol of Citrus Blackfly, Aleurocanthus woglumi Ashby (Homoptera: Aleyrodidae), by Spraying Aschersonia sp. Conidia Collected from Infected Nymphs in Quintana Roo, Mexico","authors":"O. Pérez-González, R. Gomez-flores, P. Tamez-guerra","doi":"10.1653/024.105.0311","DOIUrl":"https://doi.org/10.1653/024.105.0311","url":null,"abstract":"Summary Among the citrus production industry, Mexico represents the fifth largest producer worldwide as of 1 Mar 2022 data (Producción de cítricos en México, Biblioteca de Publicaciones Oficiales del Gobierno de la República, Gobierno, www.gob.mx [last accessed 3 Jun 2022]). Among insect pests affecting citrus orchards, the citrus blackfly Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae) causes great economic losses to this industry. Biological control with parasitoids is used principally to manage this pest. In this study, we evaluated a simple and inexpensive technique for citrus blackfly biocontrol in citrus orchards. Conidia from the entomopathogenic fungus Aschersonia sp. were collected from citrus leaves with Aschersonia sp. infecting citrus blackfly nymphs in 1 locality in Quintana Roo State, Mexico. Conidia were suspended with vigorous shaking in a 0.1% Tween solution to achieve a concentration of about 1.0 × 108 conidia per mL and sprayed on citrus blackfly uninfected nymphs in 1 selected (high citrus blackfly population) Quintana Roo locality when relative humidity was about 95%. After about 55 d, fungus had killed the treated nymphs, and had infected and killed other citrus blackflies causing an epizootic in the application area. This technique was scaled up by citrus producers to 30 ha with similar results. During 2019, relative humidity was lower than 60%, and citrus blackfly-uninfected or Aschersonia sp.-infected nymphs were not detected. During 2020, the relative humidity was higher than 80% and we did not detect Aschersonia sp. Infected- or uninfected-citrus blackfly nymphs in the previously treated area. In conclusion, collection and application of Aschersonia sp. conidia on uninfected citrus blackfly nymphs may result in an epizootic, if relative humidity is 80% or higher, and Aschersonia sp. remains and disseminates along with this insect pest. Sumario Entre la industria de producción de cítricos, México representa el quinto productor a nivel mundial según datos del 01 de marzo del 2022 (Producción de cítricos en México, Biblioteca de Publicaciones Oficiales del Gobierno de la República, Gobierno, www.gob.mx [último accedido 3 Jun 2022]). Entre las plagas de insectos, la mosca prieta de los cítricos, Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae) puede provocar grandes pérdidas económicas a esta industria. El control biológico con parasitoides es el método más utilizado para controlar esta plaga. En este estudio, evaluamos una técnica sencilla y económica en huertos de cítricos para el control biológico de la mosca prieta de los cítricos. Se recolectaron conidios del hongo entomopatógeno Aschersonia sp. de hojas de cítricos con Aschersonia sp. infectando ninfas de mosca prieta de los cítricos, en una localidad del estado de Quintana Roo. Los conidios se suspendieron con agitación vigorosa en una solución de Tween al 0.1% para lograr sobre 1.0 × 108 conidios por mL y se aplicaron mediante aspersión sobre ninfas no infe","PeriodicalId":12297,"journal":{"name":"Florida Entomologist","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44186814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}