Folia Biologica最新文献_第2页

Cytoprotective Effects of Tauroursodeoxycholic Acid in Heat Stress-Induced Endoplasmic Reticulum Stress in Male Germ Cells. 牛磺酸去氧胆酸对热应激诱导的雄性生殖细胞内质网应激的细胞保护作用。

IF 1.8 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0007

Suna Karadeniz Saygılı, Orhan Özatik, Fikriye Yasemin Özatik, Ahmet Koçak, Ayşe Çakır Gündoğdu, Mustafa Öztatlici, Mahmut Kemal Ozbilgin

{"title":"Cytoprotective Effects of Tauroursodeoxycholic Acid in Heat Stress-Induced Endoplasmic Reticulum Stress in Male Germ Cells.","authors":"Suna Karadeniz Saygılı, Orhan Özatik, Fikriye Yasemin Özatik, Ahmet Koçak, Ayşe Çakır Gündoğdu, Mustafa Öztatlici, Mahmut Kemal Ozbilgin","doi":"10.14712/fb2026.0007","DOIUrl":"10.14712/fb2026.0007","url":null,"abstract":"Heat stress disrupts cellular homeostasis in cells by inducing protein misfolding, activating endoplasmic reticulum stress pathways and impairing cell survival mechanisms. The present study aimed to evaluate the cytoprotective effects of tauroursodeoxycholic acid (TUDCA) against heat stress-induced cellular damage in an in vitro spermatogonial GC1 cell model. The IC50 concentration of TUDCA was determined by the MTT assay. GC1 cells were divided into four groups: control (GC1C), TUDCA-treated (GC1TUDCA), heat stress model (HSM; GC1HSM) and TUDCA-treated HSM (GC1HSMTUDCA). Standard culture conditions were used for GC1C. HSM group cells were incubated for 60 minutes at 43 °C. TUDCA groups of cells were treated with 200 µM TUDCA in culture media for 24 hours. Following the experiments, all groups were subjected to immuno-cytochemical staining with phosphorylated PERK (p-PERK) and C/EBP homologous protein (CHOP) antibodies, and immunoreactivity was evaluated using the H-Score system. The MTT assay determined the IC50 value of TUDCA as 558 µM for GC1 cells, with 200 µM selected for subsequent experiments due to minimal cytotoxicity. Heat stress exposure was associated with a reduction in GC1 cell viability and an increase in p-PERK and CHOP expression compared with the GC1C group. In heat-stressed cells, TUDCA treatment was associated with a significant decrease in p-PERK and CHOP expression relative to the GC1HSM group. These results suggest that TUDCA might reduce the cellular reactions related to endoplasmic reticulum stress in heat-stressed GC1 spermatogonial cells under in vitro conditions.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 2","pages":"68-74"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147981418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction-Dependent Immunomodulatory and Angiogenesis-Related Molecular Responses of Nigella sativa Oil in Adipose-Derived Mesenchymal Stem Cells. 黑油在脂肪源间充质干细胞中提取依赖的免疫调节和血管生成相关分子反应。

IF 1.8 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0004

Badel Ince, Gokturk Avsar, Cagatay Han Turkseven, Pelin Eroglu, Gamze Ayar, Murat Eser Akyurek

{"title":"Extraction-Dependent Immunomodulatory and Angiogenesis-Related Molecular Responses of Nigella sativa Oil in Adipose-Derived Mesenchymal Stem Cells.","authors":"Badel Ince, Gokturk Avsar, Cagatay Han Turkseven, Pelin Eroglu, Gamze Ayar, Murat Eser Akyurek","doi":"10.14712/fb2026.0004","DOIUrl":"10.14712/fb2026.0004","url":null,"abstract":"Nigella sativa black cumin oil (BCO) exhibits well-documented anti-inflammatory and antioxidant properties; however, the impact of extraction-related compositional variation on its cellular and molecular effects in stem cell systems remains insufficiently characterized. This study investigated the effects of cold-pressed (CP) and supercritical CO2 (ScCO2)-extracted BCO on the viability, inflammatory response and angiogenic potential of adipose-derived mesenchymal stem cells (ASCs). The oil composition and antioxidant activity were assessed using GC/MS and DPPH assays, respectively. ASCs were treated with different volumes of BCO, and cell viability was evaluated at 24, 48 and 72 h using the MTT assay. Anti-inflammatory effects were evaluated by real-time PCR analysis of IL6 and IL10 mRNA expression, while angiogenesis-related molecular responses were evaluated based on VEGF mRNA expression. Both CP and ScCO2-extracted BCO significantly increased ASC viability in a volume-dependent manner, with the highest viability consistently observed at 100 µl (P < 0.05). Treatment with 50 µl and 100 µl of either oil significantly reduced IL6 expression and concomitantly increased IL10 expression at 24 and 48 h (P < 0.05). VEGF expression was also significantly up-regulated at these time points, with ScCO2-extracted BCO inducing a more pronounced and sustained angiogenic response (P < 0.05). These findings indicate that BCO enhances ASC via-bility and molecular responses in a volume-dependent manner, while differences associated with extraction-related compositional variation may modulate inflammation- and angiogenesis-related molecular signalling in ASCs.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 1","pages":"44-53"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147689196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism Study and Analysis of Correlation between miR-409-3p and Post-Stroke Cognitive Impairment. miR-409-3p与脑卒中后认知功能障碍的相关机制研究及分析

IF 1.8 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0012

Shuzhen Fang, Ning Ning, Limin Yan, Ying Xiao, Mingming Dai, Shengquan Qin, Lin Chen, Xiulan Zhou, Chunfang Zhang

{"title":"Mechanism Study and Analysis of Correlation between miR-409-3p and Post-Stroke Cognitive Impairment.","authors":"Shuzhen Fang, Ning Ning, Limin Yan, Ying Xiao, Mingming Dai, Shengquan Qin, Lin Chen, Xiulan Zhou, Chunfang Zhang","doi":"10.14712/fb2026.0012","DOIUrl":"https://doi.org/10.14712/fb2026.0012","url":null,"abstract":"Post-stroke cognitive impairment (PSCI) is a common sequela that occurs after ischaemic stroke (IS). This study aimed to investigate whether miR-409-3p is related to PSCI. Patients with IS were divided into two subgroups: PSCI and post-stroke cognitive normality (PSCN). The plasma level of miR-409-3p was determined by RT-qPCR. The association between PSCI and miR-409-3p was evaluated through binary logistic regression and by analysing the correlation between miR-409-3p and the MoCA score. In mice with middle cerebral artery occlusion (MCAO), the effects of miR-409-3p on cognitive function were explored through mNSS score and Morris water maze. In OGD/R-induced SH-SY5Y cells, the effects of miR-409-3p on cell viability, apoptosis and neuronal inflammation were evaluated using CCK-8, flow cytometry and ELISA. The content of miR-409-3p in patients with IS and those with PSCI was both increased, and its content showed a significant negative correlation with the MoCA score. The binary logistic regression analysis showed that a high risk of PSCI was associated with miR-409-3p. In MCAO mice, inhibition of miR-409-3p can significantly reduce the mNSS score and shorten the escape latency in the Morris water maze. Further-more, in neurons induced by OGD/R, down-regulation of miR-409-3p can exert a significant protective effect on neurons, manifested by enhanced cell viability, reduced apoptosis rate and inhibition of the synthesis of inflammatory factors. We conclude that miR-409-3p is a risk factor associated with PSCI. In MCAO mice and neurons induced by OGD/R, inhibition of miR-409-3p significantly alleviated neurological deficits and suppres-sed neuronal apoptosis and neuronal inflammation.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 3","pages":"118-127"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148283608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing. miR-339-3p在骨折延迟愈合中的诊断价值及调控机制

IF 1.8 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0016

Congcong Fang, Qingqing Li, Yufang Zhao, Chunhui Liu

{"title":"The Diagnostic Value and Regulatory Mechanism of miR-339-3p in Delayed Fracture Healing.","authors":"Congcong Fang, Qingqing Li, Yufang Zhao, Chunhui Liu","doi":"10.14712/fb2026.0016","DOIUrl":"https://doi.org/10.14712/fb2026.0016","url":null,"abstract":"Delayed fracture healing (DFH) poses a considerable challenge in the clinical treatment of fractures, imposing substantial negative impacts on patients. This study intends to determine the diagnostic significance of miR-339-3p in DFH and further analyse its molecular mechanism in osteoblast differentiation. A total of 98 patients with DFH and 106 patients with normal fracture healing provided serum samples and baseline data for this study. The diagnostic value of miR-339-3p in DFH was assessed via logistic regression analysis and ROC curve. Employing MC3T3-E1 as the model cells for osteogenic differentiation, cell experiments were carried out. The expression levels of insulin-like growth factor 2 (IGF2), miR-339-3p and osteogenic differentiation markers were detected using the RT-qPCR method and Western blot assays. Cell viability and apoptosis were examined using the CCK8 assay and flow cytometry. The target genes of miR-339-3p were verified through dual-luciferase reporter assays. In patients with DFH, the expression of miR-339-3p was up-regulated, whereas the expression of IGF2 was down-regulated. miR-339-3p served as a potential biomarker for DFH and exhibited a negative correlation with IGF2. IGF2 was identified as the target gene of miR-339-3p. Inhibition of miR-339-3p could up-regulate IGF2 expression, enhance the viability and differentiation of MC3T3-E1 cells, and suppress their apoptosis. Up-regulation of miR-339-3p exerted the opposite effect. On this foundation, an increase in IGF2 could reverse this tendency. miR-339-3p demonstrates diagnostic potential for DFH. Inhibiting the expression of miR-339-3p can enhance cell activity and osteogenic differentiation through negative regulation of IGF2, thus suppressing the onset of DFH.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 4","pages":"153-162"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149180385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between circ_0092576 and Arterial Stenosis Severity and Inflammatory Status in Patients with Coronary Heart Disease and Its Clinical Value. circ_0092576与冠心病患者动脉狭窄程度及炎症状态的相关性及其临床价值

IF 1.8 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0011

Wenqin Dai, Lingli Wang, Yuan Li, Li Liu, Peng Chen

{"title":"Correlation between circ_0092576 and Arterial Stenosis Severity and Inflammatory Status in Patients with Coronary Heart Disease and Its Clinical Value.","authors":"Wenqin Dai, Lingli Wang, Yuan Li, Li Liu, Peng Chen","doi":"10.14712/fb2026.0011","DOIUrl":"https://doi.org/10.14712/fb2026.0011","url":null,"abstract":"This study aims to investigate the predictive value of circ_0092576 for coronary heart disease (CHD) and major adverse cardiac events (MACE) risk, and to elucidate its association with the degree of coronary artery stenosis and inflammatory status. A total of 200 CHD patients and 180 non-CHD controls were recruited. Plasma levels of circ_0092576, IL-1β, TNF-α and IL-10 were measured using RT-qPCR. Coronary artery stenosis severity was assessed using the Gensini score system, and MACE occurrence was recorded during regular follow-up. circ_0092576 levels were significantly higher in CHD patients than in the control group, with an area under the ROC curve (AUC) of 0.885. Furthermore, circ_0092576 showed significant positive correlations with Gensini scores and pro-inflammatory factors IL-1β and TNF-α while exhibiting a negative correlation with the anti-inflammatory factor IL-10. Follow-up revealed significantly elevated circ_0092576 levels in patients who developed MACE. Compared to the low circ_0092576 group, the high circ_0092576 group exhibited a significantly higher cumulative incidence of MACE. Elevated circ_0092576 levels correlate with increased risk of CHD and subsequent MACE. In summary, circ_0092576 is a potential biomarker for CHD risk and prognosis. Elevated levels indicate increased risk of CHD, more severe coronary artery stenosis, stronger inflammation, and are associated with an increased risk of adverse cardiac events.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 3","pages":"109-117"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148283614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wip1 Inhibition Enhances the Therapeutic Efficacy of Palbociclib in Luminal A Breast Cancer by Modulating Cell Cycle Progression and Apoptosis. Wip1抑制通过调节细胞周期进程和凋亡增强帕博西尼治疗Luminal A乳腺癌的疗效。

IF 1.8 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0006

Gökhan Çolak, Nazllcan Kaygusuz, Nezih Meydan, Esin Oktay, Mehtap Kılıc Eren

{"title":"Wip1 Inhibition Enhances the Therapeutic Efficacy of Palbociclib in Luminal A Breast Cancer by Modulating Cell Cycle Progression and Apoptosis.","authors":"Gökhan Çolak, Nazllcan Kaygusuz, Nezih Meydan, Esin Oktay, Mehtap Kılıc Eren","doi":"10.14712/fb2026.0006","DOIUrl":"10.14712/fb2026.0006","url":null,"abstract":"Cyclin-dependent kinase 4/6 (CDK4/6) inhibitors such as palbociclib have improved the treatment of hormone receptor-positive, luminal A breast cancer; however, therapeutic resistance remains a major challenge. Wild-type p53-induced phosphatase 1 (Wip1) is a negative regulator of the p53 pathway and is often over-expressed in luminal breast cancers. This study aimed to determine whether Wip1 inhibition enhances the anti-proliferative and pro-apoptotic effects of the CDK4/6 inhibitor palbociclib in luminal A breast cancer cells and to elucidate the underlying cell cycle-related mechanisms. MCF-7 cells were treated with palbociclib and Wip1 inhibitor GSK2830371, alone or in combination. Cell viability was assessed using the WST-1 assay, cell cycle distribution was analysed by flow cytometry, and apoptosis was evaluated using Annexin V/7-aminoactinomycin D staining. Expression of cell cycle regulators (CDK2, CDK4, CDK6, cyclin D1/D3, Rb, phospho-Rb) and p53-related proteins (p53, phospho-p53 Ser15, p21, p27) was determined by Western blot analysis. The combined treatment produced a concentration-dependent reduction in cell viability and a marked increase in both early and late apoptotic populations compared with monotherapies. While palbociclib alone induced G1 arrest, co-treatment with GSK2830371 shifted cells toward G2 accumulation. This was accompanied by enhanced phosphorylation of p53, up-regulation of p21 and p27, and dephosphorylation of Rb, indicating dual checkpoint engagement. These findings demonstrate that Wip1 inhibition augments palbociclib-mediated cell cycle arrest and apoptosis through modulation of the p53-Rb axis. The dual blockade of Wip1 and CDK4/6 may represent a promising therapeutic strategy for p53-proficient luminal A breast cancer.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 2","pages":"59-67"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147981366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-335-3p Alleviates Adipogenesis and Inflammation in Bone Marrow-Derived Mesenchymal Stem Cells in Osteoporotic Fractures. MiR-335-3p减轻骨质疏松性骨折中骨髓间充质干细胞的脂肪生成和炎症。

IF 1.8 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0015

Qi Chen, Wen-Hua Lu, Wei-Bing Chen

{"title":"MiR-335-3p Alleviates Adipogenesis and Inflammation in Bone Marrow-Derived Mesenchymal Stem Cells in Osteoporotic Fractures.","authors":"Qi Chen, Wen-Hua Lu, Wei-Bing Chen","doi":"10.14712/fb2026.0015","DOIUrl":"https://doi.org/10.14712/fb2026.0015","url":null,"abstract":"Osteoporotic fractures (OPF) represent a substantial share of all fracture types, seriously affecting the quality of life. Therefore, it is essential to find a biomarker for early diagnosis and prevention of OPF. Detection of mRNA expression was performed using RT-qPCR. The diagnostic value of miR-335-3p in OPF was examined by the ROC curve. Pearson's correlation analysis was employed to explore the correlation. The rat model of OPF was established by bilateral oophorectomy (OVX) and creation of a fracture in the middle of the left femur. Then, bone marrow-derived mesenchymal stem cells (BMSCs) were isolated from the rats. MiR-335-3p was over-expressed or inhibited by transfection in BMSCs. Adipogenic factors, inflammatory factors, osteogenic markers and cell metabolic activity were detected in BMSCs. MiR-335-3p expression was markedly reduced in patients with OPF, and this molecule exhibited favorable diagnostic efficacy for OPF. In OPF, miR-335-3p was positively correlated with bone mineral density. In BMSCOPF, miR-335-3p reduced inflammatory factors and increased cell metabolic activity. In addition, the over-expression of miR-335-3p reduced adipogenic factors and increased osteogenic markers. In conclusion, miR-335-3p was decreased in OPF and may have high diagnostic potential in OPF. In BMSCOPF, miR-335-3p is downregulated in OPF and possesses promising diagnostic value. It can inhibit adipogenic differentiation and inflammatory responses while promoting osteogenic differentiation in BMSCs. These findings suggest that miR-335-3p may participate in the pathogenesis of OPF and be associated with fracture healing.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 3","pages":"144-151"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148283652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-200a-5p Is a Potential Prognostic Marker for Lung Adenocarcinoma: Based on Transcriptomics, Bioinformatics, and In Vitro and In Vivo Experiments. MiR-200a-5p是肺腺癌的潜在预后标志物：基于转录组学、生物信息学和体外和体内实验

IF 1.8 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0003

Jiye Liu, Jiachun Li, Yu Liu

{"title":"MiR-200a-5p Is a Potential Prognostic Marker for Lung Adenocarcinoma: Based on Transcriptomics, Bioinformatics, and In Vitro and In Vivo Experiments.","authors":"Jiye Liu, Jiachun Li, Yu Liu","doi":"10.14712/fb2026.0003","DOIUrl":"10.14712/fb2026.0003","url":null,"abstract":"Lung adenocarcinoma is a prevalent cancer worldwide. MicroRNAs (miRNAs) are key regulators in various cancers, with miR-200a-5p emerging as a potential candidate due to its possible role in lung adenocarcinoma. However, its exact mechanisms of action remain unclear. We performed transcriptome sequencing of 32 clinical pairs to identify differentially expressed miRNAs. miR-200a-5p expression was validated in tissues and cell lines (H1975, A549) using bioinformatics and RT-qPCR. Biological effects were assessed via CCK8, EdU, Transwell, scratch assays, flow cytometry, and Western blotting (phosphorylated transducer and activator of transcription 3, fibronectin, matrix metallopeptidase 9, proliferating cell nuclear antigen, and Wnt pathway-related proteins). A protein-protein interaction network predicted downstream targets, focusing on the Wnt pathway. In vivo experiments, immunohistochemistry, and Western blotting evaluated tumour growth and Ki-67/Wnt protein expression. We found that miR-200a-5p was significantly up-regulated in lung adenocarcinoma tissues and cells, correlating with poor prognosis. Up-regulation enhanced proliferation, migration and invasion while inhibiting apoptosis in vitro. Down-regulation produced opposite effects. In vivo, miR-200a-5p promoted tumour growth via Wnt pathway activation. We conclude that miR-200a-5p acts as an oncogenic factor in lung adenocarcinoma by facilitating progression through the Wnt pathway. These findings suggest its potential as a therapeutic target and prognostic biomarker.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 1","pages":"27-43"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147689218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intravitreal Application of Mesenchymal Stem Cell By-Products Does Not Ameliorate Experimental Autoimmune Uveitis. 玻璃体内应用间充质干细胞副产物不能改善实验性自身免疫性葡萄膜炎。

IF 1.8 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0010

Eva Uherková, Kateřina Palacká, Eva Škrlová, Barbora Heřmánková, Aneta Klímová, Petra Svozílková, Vladimír Holáň, Jarmila Heissigerová

{"title":"Intravitreal Application of Mesenchymal Stem Cell By-Products Does Not Ameliorate Experimental Autoimmune Uveitis.","authors":"Eva Uherková, Kateřina Palacká, Eva Škrlová, Barbora Heřmánková, Aneta Klímová, Petra Svozílková, Vladimír Holáň, Jarmila Heissigerová","doi":"10.14712/fb2026.0010","DOIUrl":"10.14712/fb2026.0010","url":null,"abstract":"The study aimed to evaluate the therapeutic effects of mesenchymal stem cells and their by-products, including conditioned medium, extract and exosomes, on intraocular inflammation in experimental autoimmune uveitis. Uveitis was induced in the C57BL/6J mouse strain by administration of interphotoreceptor retinoid-binding protein, complete Freund's adjuvant and pertussis toxin. Mesenchymal stem cell-derived products (conditioned medium, extract and exosomes) were prepared and administered intravitreally at varying time points post-induction, primarily on day 14. Retinal inflammatory changes were monitored using fundus imaging, flow cytometry and RT-PCR analysis to evaluate clinical manifestation of inflammation, immune cell infiltration and gene expression of inflammatory markers. In vivo, administration of mesenchymal stem cell by-products did not ameliorate experimental autoimmune uveitis. On the contrary, treated eyes exhibited exacerbated inflammation, including increased immune cell infiltration and up-regulated pro-inflammatory gene expression (e.g., Gfap, Iba1, Il1b and Il17). In vitro studies suggested a trend towards anti-inflammatory effects of the conditioned medium, but these findings were not replicated in vivo. Control experiments on healthy eyes indicated that intravitreal trauma alone significantly contributed to inflammatory responses, irrespective of the substance injected. Intravitreal application of mesenchymal stem cell by-products did not demonstrate therapeutic benefits in the experimental autoimmune uveitis model and instead promoted inflammation. The results highlight the impact of administration-induced trauma and suggest that alternative delivery methods, such as systemic administration, may be more effective for mesenchymal stem cell-based therapies.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 2","pages":"95-108"},"PeriodicalIF":1.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147981435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breast Milk as a Source of Prebiotic Human Milk Oligosaccharides and Bacteria from the Lactobacillaceae Family. 母乳作为益生元母乳低聚糖和乳酸菌科细菌的来源。

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071010044

Kataryzna Łubiech, Magdalena Twarużek, Elena Sinkiewicz-Darol, Dorota Martysiak-Żurowska, Barbara Kusznierewicz

{"title":"Breast Milk as a Source of Prebiotic Human Milk Oligosaccharides and Bacteria from the Lactobacillaceae Family.","authors":"Kataryzna Łubiech, Magdalena Twarużek, Elena Sinkiewicz-Darol, Dorota Martysiak-Żurowska, Barbara Kusznierewicz","doi":"10.14712/fb2025071010044","DOIUrl":"https://doi.org/10.14712/fb2025071010044","url":null,"abstract":"Breast milk, as the optimal food for infants and young children, contains all the components necessary for proper growth and development. It is a rich source of both essential nutrients and biologically active factors, making breast milk a unique food with scientifically proven health-promoting properties. Among the entire range of biologically active factors, breast milk microorganisms and prebiotic factors, in the form of breast milk oligosaccharides, occupy an important place. The aim of our research was to determine the occurrence of bacteria with probiotic potential, belonging to the Lactobacillaceae family, in the environment of breast milk and breast milk oligosaccharides. The study included 63 human milk samples from breastfeeding women at various stages of lactation. Microorganism identification based on culture tests and MALDI TOF/MS, macronutrient analysis using the MIRIS human milk analyser, as well as analysis of human milk oligosaccharides using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry were performed. The results have shown that breast milk from different breastfeeding women is characterized by great diversity in terms of the presence of Lacto-bacillaceae bacteria in its microbiological composition. These bacteria were present in 22.2 % of the tested breast milk samples. Analysis of the human milk oligosaccharide profile revealed a slightly higher content of prebiotic factors in breast milk samples containing Lactobacillaceae, including 2'-fucosyllactose, oligosaccharide occurring in the highest amount in breast milk.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 1","pages":"44-53"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0