Folia Biologica最新文献

Short-Term Lymphocyte Culture Improves the Diagnostic Yield of Targeted RNA NGS in Cancer Predisposition Testing. 短期淋巴细胞培养提高靶向RNA NGS在癌症易感性检测中的诊断率。

IF 0.6 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0001

Marta Černá, Kateřina Matějková, Taťána Ptáčková, Petr Nehasil, Romana Mihalová, Kamila Veselá, Markéta Janatová, Jana Soukupová, Petra Kleiblová

{"title":"Short-Term Lymphocyte Culture Improves the Diagnostic Yield of Targeted RNA NGS in Cancer Predisposition Testing.","authors":"Marta Černá, Kateřina Matějková, Taťána Ptáčková, Petr Nehasil, Romana Mihalová, Kamila Veselá, Markéta Janatová, Jana Soukupová, Petra Kleiblová","doi":"10.14712/fb2026.0001","DOIUrl":"https://doi.org/10.14712/fb2026.0001","url":null,"abstract":"Although next-generation RNA sequencing (RNA-seq) is increasingly incorporated into germline cancer predisposition testing, its diagnostic utility is often limited by low expression of many clinically relevant genes. To improve RNA yield and transcript representation for targeted RNA-seq, we optimized a simple protocol based on short-term lymphocyte culture prepared directly from whole blood collected in Li-heparin tubes. We systematically evaluated biological reproducibility and pre-analytical sample handling variability and demonstrated that whole blood can be stored at 4 °C for up to 5 days prior to lymphocyte cultivation without compromising RNA quality/gene expression. Gene expression was comparable for RNA isolated from K2EDTA and Tempus tubes, whereas short-term lymphocyte culture resulted in a substantial increase in expression of clinically important genes including BRCA1, BRCA2, RAD51C, RAD51D, PALB2, CHEK2, and multiple Fanconi anaemia genes otherwise low expressed in whole blood. Cultivation for 3-5 days did not significantly affect lymphocyte gene expression, providing flexibility for routine dia-gnostics. The protocol also enables inhibition of nonsense-mediated decay to facilitate analysis of variants causing premature termination. As a proof of principle, we characterized the splicing impact of FANCA c.2602-3C>G variant (located in intron 27) using cultured lymphocytes from its carrier. The variant causes deletion of six nucleotides in the mature transcript (ΔE28p(-6)/r.2602_2607del), resulting in an in-frame deletion (p. Gln869_Phe870del). This spliceogenic effect was reliably detectable only in cultured lymphocytes preferentially expressing the full-length FANCA transcript. Overall, short-term lymphocyte culture re-presents a simple and flexible RNA source that enhances variant interpretation in clinical RNA-seq analyses.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 1","pages":"16-26"},"PeriodicalIF":0.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147689144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting FLT-3 Mutations in Acute Myeloid Leukaemia: from Molecular Insights to Clinical Strategies. 靶向FLT-3突变在急性髓性白血病：从分子的见解到临床策略。

IF 0.6 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0002

Mohammadreza Afshari, Martina Řezáčová, Darina Muthná

{"title":"Targeting FLT-3 Mutations in Acute Myeloid Leukaemia: from Molecular Insights to Clinical Strategies.","authors":"Mohammadreza Afshari, Martina Řezáčová, Darina Muthná","doi":"10.14712/fb2026.0002","DOIUrl":"https://doi.org/10.14712/fb2026.0002","url":null,"abstract":"FMS-like tyrosine kinase 3 (FLT-3) mutations represent one of the most common genetic anomalies in acute myeloid leukaemia (AML), particularly in adults. The two most common types of mutations, internal tandem duplications (ITD) and tyrosine kinase domain (TKD) point mutations, facilitate uncontrolled cellular proliferation and unfavourable patient outcomes. These mutations are linked with a high relapse rate and shorter overall survival, highlighting the need for targeted therapies to be used. Recent advances in the discovery of new agents enabled incorporation of FLT-3 inhibitors into the frontline treatment regimen. First-generation inhibitors, such as midostaurin, provided the foundation for targeted therapy, while recently developed agents such as gilteritinib and quizartinib have shown more selectivity and demonstrated superior clinical efficiency and improved tolerability. This review discusses the significance of FLT-3 mutations, the evolution of targeted therapies, current treatment guidelines, and ongoing challenges such as resistance and high relapse rates. We also discuss the emerging combinations of therapies and novel agents currently in clinical trials that aim to overcome resistance and improve long-term outcomes for patients with FLT-3-mutated AML.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 1","pages":"1-15"},"PeriodicalIF":0.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147689223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

To the Maximal Nuclear Diameter and Cell Cycle Phase of Lymphoblasts or Myeloblasts in Patients Suffering from Acute Lymphoblastic and Myeloblastic Leukaemia. A Simple Morphological and Technical Note. 急性淋巴母细胞和髓母细胞白血病患者淋巴母细胞和髓母细胞的最大核直径和细胞周期期。一个简单的形态学和技术注释。

IF 0.6 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0005

Karel Smetana, Hana Klamová, Radka Šimečková, Dana Mikulenková, Josef Karban, Marek Trněný, Cyril Šálek

{"title":"To the Maximal Nuclear Diameter and Cell Cycle Phase of Lymphoblasts or Myeloblasts in Patients Suffering from Acute Lymphoblastic and Myeloblastic Leukaemia. A Simple Morphological and Technical Note.","authors":"Karel Smetana, Hana Klamová, Radka Šimečková, Dana Mikulenková, Josef Karban, Marek Trněný, Cyril Šálek","doi":"10.14712/fb2026.0005","DOIUrl":"https://doi.org/10.14712/fb2026.0005","url":null,"abstract":"The present study was undertaken to provide complementary information on the cell cycle of leukaemic lymphoblasts and myeloblasts based on the computer-assisted maximal nuclear diameter measurements. The measurements were carried out in \"one-size cell layer\" loci of bone marrow smears of patients suffering from B-cell acute lymphoblastic leukaemia (B-ALL), acute myeloblastic leukaemia with minimal differentiation (M0 AML), acute myeloblastic leukaemia without maturation (M1 AML), acute myeloblastic leukaemia with maturation (M2 AML) and chronic myeloid leukaemia (CML). The maximal nuclear diameter was also measured in peripheral blood mature lymphocytes of B-cell chronic lymphocytic leukaemia (CLL), which are known to be in the G0/G1 phase of the cell cycle. In contrast, the maximal nuclear diameter in lymphoblasts of patients with B-ALL was larger and reflected the S and G2 cell cycle phase. The largest incidence of myeloblasts with the smallest maximal nuclear diameter was in M0 AML. The smaller incidence of such cells was also noted in patients with M1 AML. Myeloblasts with larger nuclear bodies were present in M2 AML and CML. Thus, post-mitotic myeloblasts in G0 and G1 phase were characteristic of M0 and to a smaller extent of M1 AML. Myeloblasts with larger maximal nuclear diameter reflecting the S and G2 phase were dominant in M2 AML and CML. In summary, the nuclear size heterogeneity of leukaemic lymphoblasts and myeloblasts in bone marrow smears depends on various phases of the cell cycle classified according to the maximal nuclear diameter.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 1","pages":"54-57"},"PeriodicalIF":0.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147689158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction-Dependent Immunomodulatory and Angiogenesis-Related Molecular Responses of Nigella sativa Oil in Adipose-Derived Mesenchymal Stem Cells. 黑油在脂肪源间充质干细胞中提取依赖的免疫调节和血管生成相关分子反应。

IF 0.6 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0004

Badel Ince, Gokturk Avsar, Cagatay Han Turkseven, Pelin Eroglu, Gamze Ayar, Murat Eser Akyurek

{"title":"Extraction-Dependent Immunomodulatory and Angiogenesis-Related Molecular Responses of Nigella sativa Oil in Adipose-Derived Mesenchymal Stem Cells.","authors":"Badel Ince, Gokturk Avsar, Cagatay Han Turkseven, Pelin Eroglu, Gamze Ayar, Murat Eser Akyurek","doi":"10.14712/fb2026.0004","DOIUrl":"https://doi.org/10.14712/fb2026.0004","url":null,"abstract":"Nigella sativa black cumin oil (BCO) exhibits well-documented anti-inflammatory and antioxidant properties; however, the impact of extraction-related compositional variation on its cellular and molecular effects in stem cell systems remains insufficiently characterized. This study investigated the effects of cold-pressed (CP) and supercritical CO2 (ScCO2)-extracted BCO on the viability, inflammatory response and angiogenic potential of adipose-derived mesenchymal stem cells (ASCs). The oil composition and antioxidant activity were assessed using GC/MS and DPPH assays, respectively. ASCs were treated with different volumes of BCO, and cell viability was evaluated at 24, 48 and 72 h using the MTT assay. Anti-inflammatory effects were evaluated by real-time PCR analysis of IL6 and IL10 mRNA expression, while angiogenesis-related molecular responses were evaluated based on VEGF mRNA expression. Both CP and ScCO2-extracted BCO significantly increased ASC viability in a volume-dependent manner, with the highest viability consistently observed at 100 µl (P < 0.05). Treatment with 50 µl and 100 µl of either oil significantly reduced IL6 expression and concomitantly increased IL10 expression at 24 and 48 h (P < 0.05). VEGF expression was also significantly up-regulated at these time points, with ScCO2-extracted BCO inducing a more pronounced and sustained angiogenic response (P < 0.05). These findings indicate that BCO enhances ASC via-bility and molecular responses in a volume-dependent manner, while differences associated with extraction-related compositional variation may modulate inflammation- and angiogenesis-related molecular signalling in ASCs.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 1","pages":"44-53"},"PeriodicalIF":0.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147689196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-200a-5p Is a Potential Prognostic Marker for Lung Adenocarcinoma: Based on Transcriptomics, Bioinformatics, and In Vitro and In Vivo Experiments. MiR-200a-5p是肺腺癌的潜在预后标志物：基于转录组学、生物信息学和体外和体内实验

IF 0.6 4区医学

Folia Biologica Pub Date : 2026-01-01 DOI: 10.14712/fb2026.0003

Jiye Liu, Jiachun Li, Yu Liu

{"title":"MiR-200a-5p Is a Potential Prognostic Marker for Lung Adenocarcinoma: Based on Transcriptomics, Bioinformatics, and In Vitro and In Vivo Experiments.","authors":"Jiye Liu, Jiachun Li, Yu Liu","doi":"10.14712/fb2026.0003","DOIUrl":"https://doi.org/10.14712/fb2026.0003","url":null,"abstract":"Lung adenocarcinoma is a prevalent cancer worldwide. MicroRNAs (miRNAs) are key regulators in various cancers, with miR-200a-5p emerging as a potential candidate due to its possible role in lung adenocarcinoma. However, its exact mechanisms of action remain unclear. We performed transcriptome sequencing of 32 clinical pairs to identify differentially expressed miRNAs. miR-200a-5p expression was validated in tissues and cell lines (H1975, A549) using bioinformatics and RT-qPCR. Biological effects were assessed via CCK8, EdU, Transwell, scratch assays, flow cytometry, and Western blotting (phosphorylated transducer and activator of transcription 3, fibronectin, matrix metallopeptidase 9, proliferating cell nuclear antigen, and Wnt pathway-related proteins). A protein-protein interaction network predicted downstream targets, focusing on the Wnt pathway. In vivo experiments, immunohistochemistry, and Western blotting evaluated tumour growth and Ki-67/Wnt protein expression. We found that miR-200a-5p was significantly up-regulated in lung adenocarcinoma tissues and cells, correlating with poor prognosis. Up-regulation enhanced proliferation, migration and invasion while inhibiting apoptosis in vitro. Down-regulation produced opposite effects. In vivo, miR-200a-5p promoted tumour growth via Wnt pathway activation. We conclude that miR-200a-5p acts as an oncogenic factor in lung adenocarcinoma by facilitating progression through the Wnt pathway. These findings suggest its potential as a therapeutic target and prognostic biomarker.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"72 1","pages":"27-43"},"PeriodicalIF":0.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147689218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breast Milk as a Source of Prebiotic Human Milk Oligosaccharides and Bacteria from the Lactobacillaceae Family. 母乳作为益生元母乳低聚糖和乳酸菌科细菌的来源。

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071010044

Kataryzna Łubiech, Magdalena Twarużek, Elena Sinkiewicz-Darol, Dorota Martysiak-Żurowska, Barbara Kusznierewicz

{"title":"Breast Milk as a Source of Prebiotic Human Milk Oligosaccharides and Bacteria from the Lactobacillaceae Family.","authors":"Kataryzna Łubiech, Magdalena Twarużek, Elena Sinkiewicz-Darol, Dorota Martysiak-Żurowska, Barbara Kusznierewicz","doi":"10.14712/fb2025071010044","DOIUrl":"https://doi.org/10.14712/fb2025071010044","url":null,"abstract":"Breast milk, as the optimal food for infants and young children, contains all the components necessary for proper growth and development. It is a rich source of both essential nutrients and biologically active factors, making breast milk a unique food with scientifically proven health-promoting properties. Among the entire range of biologically active factors, breast milk microorganisms and prebiotic factors, in the form of breast milk oligosaccharides, occupy an important place. The aim of our research was to determine the occurrence of bacteria with probiotic potential, belonging to the Lactobacillaceae family, in the environment of breast milk and breast milk oligosaccharides. The study included 63 human milk samples from breastfeeding women at various stages of lactation. Microorganism identification based on culture tests and MALDI TOF/MS, macronutrient analysis using the MIRIS human milk analyser, as well as analysis of human milk oligosaccharides using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry were performed. The results have shown that breast milk from different breastfeeding women is characterized by great diversity in terms of the presence of Lacto-bacillaceae bacteria in its microbiological composition. These bacteria were present in 22.2 % of the tested breast milk samples. Analysis of the human milk oligosaccharide profile revealed a slightly higher content of prebiotic factors in breast milk samples containing Lactobacillaceae, including 2'-fucosyllactose, oligosaccharide occurring in the highest amount in breast milk.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 1","pages":"44-53"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of Detachment Methods (Trypsin, Accutase, and Accumax) on the Expression of Stem Cell Markers CXCR4 and CD146. 分离方法（胰蛋白酶、精准酶和Accumax）对干细胞标记物CXCR4和CD146表达的影响

IF 0.6 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071030118

Wail Abou Assaf, Jan Schmidt, Nela Jouklová, Martin Kapitán, Karolína Jankovičová, Tomáš Soukup

{"title":"The Impact of Detachment Methods (Trypsin, Accutase, and Accumax) on the Expression of Stem Cell Markers CXCR4 and CD146.","authors":"Wail Abou Assaf, Jan Schmidt, Nela Jouklová, Martin Kapitán, Karolína Jankovičová, Tomáš Soukup","doi":"10.14712/fb2025071030118","DOIUrl":"https://doi.org/10.14712/fb2025071030118","url":null,"abstract":"Trypsin (TRY) combined with ethylenediaminetetraacetic acid (EDTA) is a widely used dissociation agent due to its efficiency and cost-effectiveness. However, its impact on preserving stem cell marker expression, such as C-X-C chemokine receptor type 4 (CXCR4) (critical for cell migration and homing) and cluster of differentiation 146 (CD146) (involved in pluripotency and angiogenesis), may be suboptimal compared to alternatives such as Accuta-se (ACC) and Accumax (ACMX), as shown previous-ly in bone marrow-derived stem cells (BM-MSCs). Limited data exist on these agents' effects on dental pulp stem cells (DPSCs). This study aims to investigate the influence of TRY, ACC, and ACMX on the expression of CXCR4 and CD146 in DPSCs. Seven characterized DPSC lines were cultured under standardized conditions and detached using TRY-EDTA, ACC, or ACMX. The expression of CXCR4 and CD146 was quantified via multicolour flow cytometry using an innovative DURAClone SC panel with supplementary anti-CXCR4 antibody. Comprehen-sive statistical analyses were performed to evaluate differences in marker preservation. No statistically significant differences in CXCR4 or CD146 expression were observed across the detachment methods (P > 0.05). ACMX consistently demonstrated margi-nally higher mean expression levels for both markers (CXCR4: 84.77 %; CD146: 93.91 %) compared to ACC (CXCR4: 83.45 %; CD146: 93.41 %) and TRY (CXCR4: 83.95 %; CD146: 92.99 %). While differences were not statistically significant, ACMX consistently yielded higher mean expression of both CXCR4 and CD146, indicating a potential advantage in preserving marker integrity during the cell detachment.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 3","pages":"118-122"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145400241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low Statin Doses Have No Effect on Plasma Concentrations of cfDNA or Muscle-Specific miRNA. 低剂量他汀类药物对血浆cfDNA或肌肉特异性miRNA浓度无影响。

IF 0.6 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025.0010

Michal Vrablík, Jaroslav A Hubáček, Kristýna Janoušková, Dana Dlouhá, Jiří Laštůvka, Věra Adámková

{"title":"Low Statin Doses Have No Effect on Plasma Concentrations of cfDNA or Muscle-Specific miRNA.","authors":"Michal Vrablík, Jaroslav A Hubáček, Kristýna Janoušková, Dana Dlouhá, Jiří Laštůvka, Věra Adámková","doi":"10.14712/fb2025.0010","DOIUrl":"10.14712/fb2025.0010","url":null,"abstract":"Statins are the most commonly prescribed lipid-lowering drugs. Around 10 % of statin users develop one of statin-associated muscle syndromes (SAMS), ranging from mild myalgia to severe life-threatening rhabdomyolysis. So far, no reliable marker of SAMS has been identified. Plasma cell-free DNA (cfDNA, of nuclear or mitochondrial origin) and microRNA (miRNA) are among the potential molecules that could reflect muscle changes induced by statins. Two groups of statin-treated subjects were included into our study. In an intensively screened branch, 15 males on 10 mg of rosuvastatin provided two samples before the treatment initiation, three subsequent at weeks 4, 8 and 12 on statin treatment, and one after four weeks of the wash-out period. The second branch included 29 subjects, with samples collected before the treatment and after 5-6 and 10-12 months of treatment. Plasma cfDNA of nuclear or mitochondrial origin and miRNAs 133a-3p, 23a-5p and 1-3p were analysed using qPCR. Plasma concentrations of cfnDNA, cfmtDNA or miRNAs did not change significantly during the statin treatment (all P values between 0.27-0.93). The release of cfDNA and miRNA is probably not significantly affected by low-dose statin therapy.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 5-6","pages":"256-261"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147289666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum - Comprehensive Analysis of Hub Telomere-Related Genes and Synovial Immune Characteristics in Osteoarthritis. 骨关节炎中心端粒相关基因和滑膜免疫特征的综合分析。

IF 0.6 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025.0011

Chunxiao Du, Weidan Xu

引用次数: 0

Determinants of Implantation Success in Pancreatic Cancer Patient-Derived Xenografts: Role of Matrigel Application, Histological Subtype, and Time Management. 胰腺癌患者来源的异种移植物移植成功的决定因素：基质应用、组织学亚型和时间管理的作用。

IF 0.6 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025.0006

Emin Gayibov, Tomáš Sychra, Alžběta Spálenková, Karolína Šeborová, Kamila Koucká, Tereza Tesařová, Kamila Kočí, Matěj Vícha, Arpád Szabó, Radka Václavíková, Zdeněk Šubrt, Pavel Souček, Martin Oliverius

{"title":"Determinants of Implantation Success in Pancreatic Cancer Patient-Derived Xenografts: Role of Matrigel Application, Histological Subtype, and Time Management.","authors":"Emin Gayibov, Tomáš Sychra, Alžběta Spálenková, Karolína Šeborová, Kamila Koucká, Tereza Tesařová, Kamila Kočí, Matěj Vícha, Arpád Szabó, Radka Václavíková, Zdeněk Šubrt, Pavel Souček, Martin Oliverius","doi":"10.14712/fb2025.0006","DOIUrl":"https://doi.org/10.14712/fb2025.0006","url":null,"abstract":"Pancreatic cancer (PC) is a growing global health concern, highlighting the need for improved preclinical models. Patient-derived xenografts (PDXs) closely replicate tumour biology and serve as a link between preclinical and clinical research. This study investigated the key factors influencing the success of PDX implantation in PC. We compared Matrigel-assisted versus Matrigel-free implantation. We also evaluated the impact of histological subtype on implantation success, analysing pancreatic ductal adenocarcinoma (PDAC), adenosquamous carcinoma (ASPC) and acinar cell carcinoma (ACC). Additionally, we assessed whether the tumour specimen culture-to-implantation period affected the take rate or tumour growth rate. A significance threshold of P < 0.05 was applied (95% confidence interval), and multivariable regression analysis was conducted to identify independent predictors of implantation success. In both NOD/SCID and NU/NU (nude) strains, Matrigel-assisted PDAC implantations achieved significantly higher take rates (75 % vs 90 %) compared to direct implantations (25 % vs 0 %) in the second generation (P = 0.02). The ASPC subtype was a significant predictor of success in the NOD/SCID strain (P = 0.04). The culture-to-implantation period did not affect the take rates. The nude strain significantly prolonged ACC engraftment (P = 0.02). In direct ACC implantations, earlier generations (F1-F5) required shorter engraftment growth duration (P < 0.0001). For ASPC, later generations demonstrated longer growth duration (P < 0.04). These findings emphasize critical variables in optimizing PC PDX protocols, particularly Matrigel use, mouse strain selection, and consideration of histological and generation-specific effects. Such refinements can optimize PDX efficiency and translational relevance.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 5-6","pages":"212-224"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147289692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0