Folia Biologica最新文献

Breast Milk as a Source of Prebiotic Human Milk Oligosaccharides and Bacteria from the Lactobacillaceae Family. 母乳作为益生元母乳低聚糖和乳酸菌科细菌的来源。

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071010044

Kataryzna Łubiech, Magdalena Twarużek, Elena Sinkiewicz-Darol, Dorota Martysiak-Żurowska, Barbara Kusznierewicz

{"title":"Breast Milk as a Source of Prebiotic Human Milk Oligosaccharides and Bacteria from the Lactobacillaceae Family.","authors":"Kataryzna Łubiech, Magdalena Twarużek, Elena Sinkiewicz-Darol, Dorota Martysiak-Żurowska, Barbara Kusznierewicz","doi":"10.14712/fb2025071010044","DOIUrl":"https://doi.org/10.14712/fb2025071010044","url":null,"abstract":"Breast milk, as the optimal food for infants and young children, contains all the components necessary for proper growth and development. It is a rich source of both essential nutrients and biologically active factors, making breast milk a unique food with scientifically proven health-promoting properties. Among the entire range of biologically active factors, breast milk microorganisms and prebiotic factors, in the form of breast milk oligosaccharides, occupy an important place. The aim of our research was to determine the occurrence of bacteria with probiotic potential, belonging to the Lactobacillaceae family, in the environment of breast milk and breast milk oligosaccharides. The study included 63 human milk samples from breastfeeding women at various stages of lactation. Microorganism identification based on culture tests and MALDI TOF/MS, macronutrient analysis using the MIRIS human milk analyser, as well as analysis of human milk oligosaccharides using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry were performed. The results have shown that breast milk from different breastfeeding women is characterized by great diversity in terms of the presence of Lacto-bacillaceae bacteria in its microbiological composition. These bacteria were present in 22.2 % of the tested breast milk samples. Analysis of the human milk oligosaccharide profile revealed a slightly higher content of prebiotic factors in breast milk samples containing Lactobacillaceae, including 2'-fucosyllactose, oligosaccharide occurring in the highest amount in breast milk.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 1","pages":"44-53"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Potential Inflammatory Role of IL-6 Signalling in Perturbing the Energy Metabolism Function by Stimulating the Akt-mTOR Pathway in Jurkat T Cells. IL-6信号在Jurkat T细胞中通过刺激Akt-mTOR通路干扰能量代谢功能的潜在炎症作用

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071010008

Abdullah Alghamdi, Mohammed Alissa

{"title":"The Potential Inflammatory Role of IL-6 Signalling in Perturbing the Energy Metabolism Function by Stimulating the Akt-mTOR Pathway in Jurkat T Cells.","authors":"Abdullah Alghamdi, Mohammed Alissa","doi":"10.14712/fb2025071010008","DOIUrl":"https://doi.org/10.14712/fb2025071010008","url":null,"abstract":"Numerous studies have reported that increased interleukin 6 (IL-6) and soluble IL-6 receptor (sIL-6) levels induce inflammatory conditions. However, the exact mechanisms by which IL-6 drives inflammatory conditions remain unclear. Therefore, we investigated the potential role of IL-6/sIL-6R in inducing energy metabolism, including glycolysis, oxidative phosphorylation, lactate secretion and Akt/mTOR phosphorylation, in Jurkat cells, and whether IL-6 would increase the risk of developing inflammatory conditions due to the high metabolic profile of the T cells. Jurkat CD4 T-cell lines were stimulated with IL-6/sIL-6R for 24 h prior to 48-h stimulation with anti-CD3/CD28. Lactate secretion, glycolysis and oxidative phosphorylation levels were characterized using the Seahorse XF analyser. The Akt and mTOR phosphorylation status was detected using Western blotting. IL-6/sIL-6R significantly induced glycolysis and oxidative phosphorylation and their related parameters, including glycolytic capacity and maximal respiration, followed by significantly increased lactate secretion. Akt and mTOR phosphorylation were increased, which could have resulted from energy metabolism. Here we show that IL-6 enhanced the metabolic profile of Jurkat cells. This effect could have consequences for the metabolism-related signalling pathways, including Akt and mTOR, suggesting that IL-6 might promote T-cell energy metabolism, where T-cell hyperactivity might increase the inflammatory disease risk. The findings should be validated using studies on primary cells isolated from humans.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 1","pages":"8-17"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Value and Regulatory Mechanism of miR-767-5p in Colorectal Cancer. miR-767-5p在结直肠癌中的临床价值及调控机制

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071010018

Ping Lin, Xiuju Qin, Caiyun Yi, Man Jiang, Lili Yi, Yuemian Liang

{"title":"Clinical Value and Regulatory Mechanism of miR-767-5p in Colorectal Cancer.","authors":"Ping Lin, Xiuju Qin, Caiyun Yi, Man Jiang, Lili Yi, Yuemian Liang","doi":"10.14712/fb2025071010018","DOIUrl":"https://doi.org/10.14712/fb2025071010018","url":null,"abstract":"The poor prognosis of colorectal cancer (CRC) contributes to a yearly increase in CRC mortality, while microRNAs (miRNAs) were found to play a regulatory function in diversiform cancers, including CRC. The objective of this research was to evaluate the clinical value and possible regulatory mechanisms of miR-767-5p in CRC. The expression level of miR-767-5p in CRC tissues and cells was examined. The Kaplan-Meier curve was utilized to analyse the function of miR-767-5p in CRC prognosis. The independent prognostic factors in CRC were assessed by a multivariate COX regression analysis. Additionally, the regulatory mechanism of miR-767-5p in CRC was determined through an in vitro cell experiment. The miR-767-5p expression was down-regulated in CRC tumour tissues and CRC cells. Indicators such as tumour differentiation, TNM, LNM and miR-767-5p were identified as independent prognostic factors for a poor CRC prognosis. The regulatory relationship between miR-767-5p and nuclear factor I A (NFIA) was verified by the dual-luciferase reporter assay, and the NFIA expression level was significantly suppressed by over-expressed miR-767-5p. The proliferation, migration and invasion of CRC cells were inhibited by over-expressing miR-767-5p, while the inhibition effect could be reversed by over-expressing NFIA. The over-expressed miR-767-5p could serve as a tumour suppressor to inhibit the progression of CRC by suppressing the expression level of NFIA.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 1","pages":"18-28"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-296-3p Controls Osteogenic Proliferation and Differentiation by Targeting ICAT and Is Involved in Fracture Healing. miR-296-3p通过靶向ICAT调控成骨增殖和分化并参与骨折愈合。

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071020064

Feng Xu, Kun Huang, Wenjun Ji, Miao Huang, Jincheng Sima, Jin Li, Hao Song, Wei Xiong, Zhong Tian

{"title":"miR-296-3p Controls Osteogenic Proliferation and Differentiation by Targeting ICAT and Is Involved in Fracture Healing.","authors":"Feng Xu, Kun Huang, Wenjun Ji, Miao Huang, Jincheng Sima, Jin Li, Hao Song, Wei Xiong, Zhong Tian","doi":"10.14712/fb2025071020064","DOIUrl":"https://doi.org/10.14712/fb2025071020064","url":null,"abstract":"Fragility fractures have been a cause for concern because of their high incidence. For the prevention and treatment of osteoporotic fractures, it is important to understand how to promote bone formation and increase bone mass. This study investigated miR-296-3p expression and function in fragility fracture. The study enrolled 98 patients with hip fractures, 90 patients with wrist fractures and 35 healthy controls. RT-qPCR was used to detect the miR-296-3p level changes before and after surgery in fracture patients and during the differentiation of human bone mesenchymal stem cells (BMSCs). The starBase bioinformatics database was used for prediction of the miR-296-3p target gene, and dual luciferase report was used for verification of the target relationship. Our results demonstrated that miR-296-3p levels are up-regulated in fracture patients, while they gradually decrease during human BMSC differentiation. The up-regulation of miR-296-3p inhibited the proliferation and differentiation ability of human BMSCs, while inhibition of its expression had the opposite effects. miR-296-3p negatively regulates osteogenic differentiation, and over-expression of inhibitor of β-catenin and TCF (ICAT) could counteract the negative regulatory effect. miR-296-3p targets ICAT and affects the expression of key proteins in the Wnt/β-catenin signalling pathway. In conclusion, miR-296-3p can regulate the division and differentiation of osteoblasts by affecting the expression of ICAT and participate in fracture healing.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 2","pages":"64-72"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-4478 Promotes Ferroptosis of Nucleus Pulposus Cells through Targeting SLC7A11 to Induce IVDD. miR-4478通过靶向SLC7A11诱导IVDD促进髓核细胞铁下垂。

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071010029

Dongliang Gong, Long Jia, Yuhang Wang, Chengwei Xu, Xuxing Sun, Xiao Wu, Xiaojun Han

{"title":"miR-4478 Promotes Ferroptosis of Nucleus Pulposus Cells through Targeting SLC7A11 to Induce IVDD.","authors":"Dongliang Gong, Long Jia, Yuhang Wang, Chengwei Xu, Xuxing Sun, Xiao Wu, Xiaojun Han","doi":"10.14712/fb2025071010029","DOIUrl":"https://doi.org/10.14712/fb2025071010029","url":null,"abstract":"Nucleus pulposus cells (NPC) are important for the development of intervertebral disc degeneration (IVDD). miR-4478 can aggravate IVDD, but whether it can aggravate IVDD by regulating ferroptosis in NPC remains unclear. The optimal level of ferroptosis activator RSL3 for eliciting ferroptosis in NPC was screened by Western blot and CCK-8 assay. The targeting relationship between miR-4478 and its potential target solute carrier family 7 member 11 (SLC7A11) was explored based on dual luciferase assay. On this basis, IVDD models were constructed. After over-expression or knockdown of miR-4478 or SLC7A11, CCK-8 and calcein-AM/PI assays were employed to evaluate cell damage. Flow cytometry, Western blot and Prussian blue staining were employed to evaluate oxidation and ferroptosis levels, and histopathological staining was applied to evaluate the intervertebral disc tissue injury degree. The optimal concentration of RSL3 was 1 μM. Under these conditions, miR-4478 or SLC7A11 can be effectively over-expressed or knocked down after transfection. Knockdown of miR-4478 can improve the survival rate of NPC, the level of Fe2+ ions, improve the pathological damage of intervertebral disc structure, reduce iron deposition in tissues, and significantly reduce expression of reactive oxygen species (ROS) and ferroptosis-related protein. The levels of antioxidant enzymes were significantly increased. When miR-4478 was over-expressed, the above phenomenon was reversed. On this basis, after SLC7A11 was over-expressed, the effect of miR-4478 up-regulation was weakened, and NPC ferroptosis was improved. miR-4478 can target SLC7A11 to promote NPC damage, peroxide accumulation and iron metabolism disorders, leading to ferroptosis, thereby inducing IVDD.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 1","pages":"29-43"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endothelium and Systemic Inflammation in Neonates and Children. 新生儿和儿童的内皮和全身性炎症。

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071020055

Petr Janec, Jana Dorňáková, Jan Živný, Jan Janota

引用次数: 0

Exploring IDH1 and IDH2 Mutations in Paediatric Medulloblastoma. 探讨儿童髓母细胞瘤中IDH1和IDH2突变。

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071020073

Motaz Fadul, Saleh Baeesa, Alaa Alkhotani, Amany A Fathaddin, Ahmed I Lary, Bassam Addass, Taghreed Alsinani, Ahmed Bamaga, Ahmad Albeshri, Mohammed M Karami, Mohammed A Abutalib, Maher Kurdi

{"title":"Exploring IDH1 and IDH2 Mutations in Paediatric Medulloblastoma.","authors":"Motaz Fadul, Saleh Baeesa, Alaa Alkhotani, Amany A Fathaddin, Ahmed I Lary, Bassam Addass, Taghreed Alsinani, Ahmed Bamaga, Ahmad Albeshri, Mohammed M Karami, Mohammed A Abutalib, Maher Kurdi","doi":"10.14712/fb2025071020073","DOIUrl":"https://doi.org/10.14712/fb2025071020073","url":null,"abstract":"Medulloblastoma (MB) in children is associated with distinct molecular subgroups, reflecting substantial biological heterogeneity. The presence of isocitrate dehydrogenase 1 (IDH1) and IDH2 mutations in paediatric MB has been rarely reported and not routinely investigated. Our study included 23 samples from paediatric patients diagnosed with MB. Hotspot alterations at codons IDH1 R132 and IDH2 R172 were examined using Sanger sequencing following polymerase chain reaction (PCR). The mean age of the patients was 10 years (SD: 4.25), comprising 17 males and 6 females. All cases exhibited classical histological features of MB. β-Catenin expression was observed in four cases (17.4 %), while 19 cases (82.6 %) showed no expression. No statistically significant differences in progression-free survival (PFS) were found between MBs with positive or negative β-catenin expression (P = 0.6). Radiotherapy alone was administered to four patients (17.4 %), while 19 patients (82.6 %) received combined radiotherapy and chemotherapy. The median PFS was 383 days (1 year and 18 days). IDH1 R132 or IDH2 R172 hotspot mutations were not detected in any of the samples. The absence of IDH1 or IDH2 mutations in paediatric MB may be attributed to differences in mutational profiles and cellular origins in childhood MB, despite its histomolecular similarities with adult MB.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 2","pages":"73-78"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semi-automated RNA Isolation from Tempus Blood RNA Tubes Using the Magcore Plus II Instrument. 使用Magcore Plus II仪器从Tempus血RNA管中半自动分离RNA。

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071020088

Marta Černá, Barbora Šťastná, Pavel Pešek, Taťána Ptáčková, Markéta Janatová, Jana Soukupová, Klára Horáčková, Petra Kleiblová

{"title":"Semi-automated RNA Isolation from Tempus Blood RNA Tubes Using the Magcore Plus II Instrument.","authors":"Marta Černá, Barbora Šťastná, Pavel Pešek, Taťána Ptáčková, Markéta Janatová, Jana Soukupová, Klára Horáčková, Petra Kleiblová","doi":"10.14712/fb2025071020088","DOIUrl":"https://doi.org/10.14712/fb2025071020088","url":null,"abstract":"High-throughput, precise and cost-effective isolation of high-quality RNA is essential for the growing number of RNA-based next-generation sequencing (NGS) analyses. Manual RNA isolation provides sufficient quality but requires significant hands-on time and carries an increased risk of contamination and sample misidentification. Here we describe a semi-automated protocol for the isolation of high-quality total RNA from 3 ml of peripheral blood collected in Tempus Blood RNA Tubes. The isolation can be performed either from the total volume of 9 ml of Tempus blood lysate or from smaller volumes (6 and 3 ml, respectively) using the MagCore triXact RNA Kit on the MagCore Plus II automated nucleic acid extractor, which allows RNA isolation in single tubes. The original isolation protocol (#631) for whole blood RNA isolation was customized by the manufacturer (#631T) by omitting the cell lysis step. After optimizing the process, we compared the yield and quality of 760 RNA samples isolated manually or by semi-automated methods. We conclude that RNA isolation using the semi-automated MagCore protocol yields 5-10 μg of total RNA from 6 ml of lysate (2 ml of peripheral blood), which is almost comparable in quantity and quality to manual isolation. In addition, we show that the remaining 3 ml of lysate is sufficient for backup re-isolation. Our semi-automated RNA protocol reduces hands-on time without increasing costs and yields bulky total RNA of a quali-ty suitable for subsequent RNA NGS applications.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 2","pages":"88-94"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Clinical Significance and Mechanistic Role of the LINC00487/hsa-miR-663b Axis in Cell Line Models of Acute Lung Injury. 探讨LINC00487/hsa-miR-663b轴在急性肺损伤细胞系模型中的临床意义及机制作用。

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071020079

Xixiang Yan, Ziqi Xie, Xiaoye Zheng, Qing Xie, Junle Yang, Xiaojuan Wu

{"title":"Exploring the Clinical Significance and Mechanistic Role of the LINC00487/hsa-miR-663b Axis in Cell Line Models of Acute Lung Injury.","authors":"Xixiang Yan, Ziqi Xie, Xiaoye Zheng, Qing Xie, Junle Yang, Xiaojuan Wu","doi":"10.14712/fb2025071020079","DOIUrl":"https://doi.org/10.14712/fb2025071020079","url":null,"abstract":"Acute lung injury (ALI) is a serious lung disease that tends to progress to acute respiratory distress syndrome (ARDS). This study was aimed to seek new biomarkers of ALI to provide a basis for monitoring the progress of ALI in time. A human bronchial epithelial cell line (HBEC3-KT) was treated with 1 μg/ml lipopolysaccharide (LPS) to induce the ALI response. The expression of LINC00487 and hsa-miR-663b in LPS-treated HBEC3-KT cells was detected by RT-qPCR. The regulation of hsa-miR-663b by LINC00487 was investigated using a dual luciferase assay and an over-expression experiment. Cell proliferation and apoptosis were detected by the CCK-8 assay and annexin V-FITC kit. Serum levels of LINC00487 and hsa-miR-663b were detected by collecting blood samples from ALI patients (with or without ARDS), and the ROC curve was constructed to assess their clinical value in ALI. LPS inhibited proliferation of HBEC3-KT cells and promoted their apoptosis and inflammatory response, which were further enhanced by LINC00487 over-expression and reversed by an hsa-miR-663b mimic. The hsa-miR-663b mimic weakened the luciferase activity of HBEC3-KT cells transfected with the luciferase vector of wild-type LINC00487. The cellular level of hsa-miR-663b was down-regulated by LINC00487 over-expression and increased by LINC00487 knockdown. The ROC curve showed that LINC00487 combined with hsa-miR-663b effectively diagnosed ALI (AUC = 0.840) and was a classifier for ALI patients with or without ARDS (AUC = 0.822). Serum LINC00487 and hsa-miR-663b levels are valuable biomarkers of ALI and can monitor the ALI progress. LINC00487 may promote ALI progression by negatively regulating hsa-miR-663b.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 2","pages":"79-87"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selected Genetic Characteristics of the Vietnamese Minority Living in the Czech Republic. 生活在捷克共和国的越南少数民族的遗传特征。

IF 1.1 4区医学

Folia Biologica Pub Date : 2025-01-01 DOI: 10.14712/fb2025071010001

Khanh Ha Pham, Jaroslav A Hubáček

{"title":"Selected Genetic Characteristics of the Vietnamese Minority Living in the Czech Republic.","authors":"Khanh Ha Pham, Jaroslav A Hubáček","doi":"10.14712/fb2025071010001","DOIUrl":"https://doi.org/10.14712/fb2025071010001","url":null,"abstract":"The aim of this study was to analyse the allelic distribution of selected genes in the Czech and Vietnamese populations. We analysed samples from 94 Vietnamese volunteers and 2,859 Czech population-based subjects (2,559 from the Czechs post-MONICA and 300 volunteers from the South region of the Czech Republic). There were significant differences between the two populations for most, but not all, of the SNPs analysed. In particular, the prevalence of risk alleles in the analysed polymorphisms tended to be lower in the Vietnamese community compared to the Czech population, especially within the FTO (rs17817449; associated with obesity risk, P < 0.0001), TCF7L2 (rs7903146; linked to type 2 dia-betes, P < 0.0001) and ADH1B (rs1229984; related to alcohol consumption, P < 0.0001) genes. The genotype within the MCM6/LCT cluster (rs4988235) associated with lactase persistence was not present in the Vietnamese population. Slight genotype differences were detected for one HFE polymorphism (rs1799945 with P = 0.005; but not for rs1800562). Only the genotype frequencies within the MC4R and APOE genes were almost identical in both populations. We conclude that the Vietnamese population may have a lower genetic predisposition to the non-communicable diseases such as obesity or diabetes mellitus.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"71 1","pages":"1-7"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0