Experimental Dermatology最新文献_第8页

CD4+ T Cells Occupy Perivascular and Perifollicular Niches in Healthy Human Skin 健康人体皮肤中占据血管周围和毛囊周围壁龛的 CD4+ T 细胞

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2024-12-13 DOI: 10.1111/exd.70023

Courtney E. Macon, Annie Yang, Dhara Patel, Jeffrey P. North, Michael D. Rosenblum, Jarish N. Cohen

{"title":"CD4+ T Cells Occupy Perivascular and Perifollicular Niches in Healthy Human Skin","authors":"Courtney E. Macon, Annie Yang, Dhara Patel, Jeffrey P. North, Michael D. Rosenblum, Jarish N. Cohen","doi":"10.1111/exd.70023","DOIUrl":"10.1111/exd.70023","url":null,"abstract":"<div>\u0000 \u0000 Regulatory T cells (Tregs) are specialised T lymphocytes that sit at the nexus of immune regulation and tissue repair. While it is appreciated that a substantial number of Tregs are present in healthy human skin, less is known about their microanatomic spatial localisation. Knowledge about the specialised niches that Tregs occupy may aid in rational drug development to treat dermatologic diseases. Thus, we performed multiplexed immunohistochemistry for CD4 and FOXP3 (the lineage-defining transcription factor of Tregs) on healthy skin sections obtained from eight different cutaneous sites, and quantified Tregs and Tcon in distinct regions. We found that Tregs (CD4+ FOXP3+) comprised roughly 20% of CD4+ T cells in skin and that Tregs and T-conventional cells (Tcon; CD4+ Foxp3−) are enriched in follicularly dense skin and show preferential accumulation in perivascular and perifollicular niches in the upper dermis. Additionally, male skin shows a significant increase in the numbers of Tregs and Tcon, while female skin shows a higher Tcon:Treg ratio. We also find that the frequency of skin Tregs declines over time. Overall, we conclude that the upper dermal perivascular region is a niche that supports the accumulation of CD4+ T cells in steady-state human skin.\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 12","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in Microbiome-Based Therapeutics for Dermatological Disorders: Current Insights and Future Directions 基于微生物组的皮肤疾病治疗的进展：当前的见解和未来的方向。

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2024-12-06 DOI: 10.1111/exd.70019

Tushar Madaan, Kyla Doan, Alexandra Hartman, Dominick Gherardini, Alec Ventrola, Yuhang Zhang, Nalinikanth Kotagiri

{"title":"Advances in Microbiome-Based Therapeutics for Dermatological Disorders: Current Insights and Future Directions","authors":"Tushar Madaan, Kyla Doan, Alexandra Hartman, Dominick Gherardini, Alec Ventrola, Yuhang Zhang, Nalinikanth Kotagiri","doi":"10.1111/exd.70019","DOIUrl":"10.1111/exd.70019","url":null,"abstract":"The human skin hosts an estimated 1000 bacterial species that are essential for maintaining skin health. Extensive clinical and preclinical studies have established the significant role of the skin microbiome in dermatological disorders such as atopic dermatitis, psoriasis, diabetic foot ulcers, hidradenitis suppurativa and skin cancers. In these conditions, the skin microbiome is not only altered but, in some cases, implicated in disease pathophysiology. Microbiome-based therapies (MBTs) represent an emerging category of live biotherapeutic products with tremendous potential as a novel intervention platform for skin diseases. Beyond using established wild-type strains native to the skin, these therapies can be enhanced to express targeted therapeutic molecules, offering more tailored treatment approaches. This review explores the role of the skin microbiome in various common skin disorders, with a particular focus on the development and therapeutic potential of MBTs for treating these conditions.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 12","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bactogram: Spatial Analysis of Bacterial Colonisation in Epidermal Wounds 杆状图：表皮伤口细菌定植的空间分析

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2024-12-03 DOI: 10.1111/exd.70018

Karl Wallblom, Fredrik Forsberg, Sigrid Lundgren, Jane Fisher, José Cardoso, Ganna Petruk, Ann-Charlotte Strömdahl, Karim Saleh, Manoj Puthia, Artur Schmidtchen

{"title":"Bactogram: Spatial Analysis of Bacterial Colonisation in Epidermal Wounds","authors":"Karl Wallblom, Fredrik Forsberg, Sigrid Lundgren, Jane Fisher, José Cardoso, Ganna Petruk, Ann-Charlotte Strömdahl, Karim Saleh, Manoj Puthia, Artur Schmidtchen","doi":"10.1111/exd.70018","DOIUrl":"https://doi.org/10.1111/exd.70018","url":null,"abstract":"Skin barrier damage and subsequent development of harmful microbiota contribute to conditions such as wound infections, atopic dermatitis and chronic wounds, which impact millions of people globally and pose a significant economic burden on healthcare systems. Established microbial sampling methods, such as swabs and tissue biopsies, provide limited information on the spatial distribution of bacteria. We here describe a new method that produces a visual map of the distribution of cultivable bacteria, denoted ‘Bactogram’, across the whole wound and surrounding skin, suitable for image-based quantification. As part of an exploratory endpoint in a clinical trial we applied the Bactogram method to 48 suction blister wounds in 24 healthy volunteers. Bacteria developed in all wounds, predominantly on the skin under the dressing and near wound edges. Two quantification methods, based on visual scoring and image analysis, demonstrated high inter-, and intra-rater agreement and were used to characterise bacterial re-colonisation during epidermal wound healing. We also demonstrated proof of concept that the method can be used with chromogenic agar to enable spatial identification of pathogenic bacterial species, such as Staphylococcus aureus. In conclusion, this study introduces a simple method for sampling bacteria over large areas and generating a bacterial map that can identify spatial variations in bacterial composition and abundance in skin and wound conditions.Trial Registration: ClinicalTrials.gov identifier: NCT05378997","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 12","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence of a Dose-Dependent Phenotypic Effect of the Desmoplakin (DSP) c.273+5G > A Variant in a Child With Palmoplantar Keratoderma and Woolly Hair Association 在一名患有掌跖角化病和毛发症的儿童身上发现的去鳞屑蛋白（DSP）c.273+5G > A 变体的剂量依赖性表型效应证据

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2024-11-25 DOI: 10.1111/exd.70012

Giulia Pascolini, Giovanni Luca Scaglione, Feliciana Mariotti, Paola Concolino, Angelo Minucci, Biagio Didona, Daniele Castiglia, Giovanni Di Zenzo

引用次数: 0

Generating Skin-Derived Precursor-Like Cells From Human-Induced Pluripotent Stem Cell-Derived Skin Organoids 从人类诱导多能干细胞衍生的皮肤器官组织中生成皮肤前体细胞

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2024-11-25 DOI: 10.1111/exd.70017

Imaan A. Ahmed, Jane Sun, Min Jie Kong, Kiarash Khosrotehrani, Abbas Shafiee

{"title":"Generating Skin-Derived Precursor-Like Cells From Human-Induced Pluripotent Stem Cell-Derived Skin Organoids","authors":"Imaan A. Ahmed, Jane Sun, Min Jie Kong, Kiarash Khosrotehrani, Abbas Shafiee","doi":"10.1111/exd.70017","DOIUrl":"https://doi.org/10.1111/exd.70017","url":null,"abstract":"<div>\u0000 \u0000 Skin-derived precursor (SKPs) cells are multipotent stem cells found in the dermis that contribute to wound healing and induce hair follicle neogenesis when transplanted. The clinical application of adult human SKPs, however, is hindered by their loss of potency after in vitro expansion. To overcome this challenge, we aimed to isolate SKPs from human-induced pluripotent stem cell-derived skin organoids (SKOs), to enable mass production of these cells for therapeutics. We developed a protocol to isolate skin-derived precursor-like cells (SKP-like cells) from human SKOs. SKP-like cells derived from SKOs exhibited characteristic spheroid morphology and were capable of self-renewal in defined SKP growth medium. Immunofluorescence analysis confirmed the expression of key markers, including SOX2, fibronectin and S100β, within the SKP-like cells. The findings of this pilot study shed light on the potential of SKO-derived SKP-like cells for future hair regenerative applications. Furthermore, this research highlights the application of human SKOs as a valuable source for isolating progenitor cells, aiming to advance hair regeneration and restore skin function.\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 11","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of ceRNA Network and Disease Diagnosis Model for Keloid Based on Tumor Suppressor ERRFI1 基于肿瘤抑制因子ERRFI1的ceRNA网络和瘢痕疙瘩疾病诊断模型的构建

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2024-11-19 DOI: 10.1111/exd.70004

Pengsheng Chen, Qingfu Su, Xingong Lin, Xianying Zhou, Wanting Yao, Xiaxinqiu Hua, Yanyan Huang, Rongrong Xie, Huiyong Liu, Chaoyang Wang

{"title":"Construction of ceRNA Network and Disease Diagnosis Model for Keloid Based on Tumor Suppressor ERRFI1","authors":"Pengsheng Chen, Qingfu Su, Xingong Lin, Xianying Zhou, Wanting Yao, Xiaxinqiu Hua, Yanyan Huang, Rongrong Xie, Huiyong Liu, Chaoyang Wang","doi":"10.1111/exd.70004","DOIUrl":"https://doi.org/10.1111/exd.70004","url":null,"abstract":"<div>\u0000 \u0000 The aim of this study is to identify the key biomarker of keloid (KD) with significant diagnostic value and to construct the related competing endogenous RNA (ceRNA) network and disease diagnostic model to provide new ideas for the early diagnosis and prevention of KD. Public databases were used to identify the key gene of KD. Enrichment analysis and immune cell infiltration (ICI) analysis revealed its functional and immune characteristics. Then, a ceRNA network was constructed to explore the potential pathways of it. Random forest (RF) analysis was applied to construct a predictive model for the disease diagnosis of KD. Finally, immunohistochemistry (IHC) and RT-qPCR were used to verify the differential expression of key gene. ERRFI1 was identified as a key biomarker in KD and was lowly expressed in KD. The ceRNA network revealed that H0TAIRM1-has-miR-148a-3p-ERRFI1 may be a potential pathway in KD. Finally, a 2-gene diagnostic prediction model (ERRFI1, HSD3B7) was constructed and externally validated and the results suggested that the model had good diagnostic performance. ERRFI1 is a downregulated gene in KD and is expected to be a promising predictive marker and disease diagnostic gene. ICI may play a role in the progression of KD. The ceRNA network may provide new clues to the potential pathogenesis of KD. Finally, the new KD diagnostic model could be an effective tool for assessing the risk of KD development.\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 11","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lysophosphatidylcholine Acyltransferase 2 Contributes to Increased Allergic and Irritant Inflammation in Mice 溶血磷脂酰胆碱酰基转移酶 2 导致小鼠过敏性和刺激性炎症加剧

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2024-11-08 DOI: 10.1111/exd.70015

Midori Kawasaki-Nagano, Risa Tamagawa-Mineoka, Tomoki Kurioka, Yukiyasu Arakawa, Mari Nakanishi, Megumi Kishida, Hiromi Nishigaki, Tomomi Hashidate-Yoshida, Hideo Shindou, Norito Katoh

{"title":"Lysophosphatidylcholine Acyltransferase 2 Contributes to Increased Allergic and Irritant Inflammation in Mice","authors":"Midori Kawasaki-Nagano, Risa Tamagawa-Mineoka, Tomoki Kurioka, Yukiyasu Arakawa, Mari Nakanishi, Megumi Kishida, Hiromi Nishigaki, Tomomi Hashidate-Yoshida, Hideo Shindou, Norito Katoh","doi":"10.1111/exd.70015","DOIUrl":"10.1111/exd.70015","url":null,"abstract":"<div>\u0000 \u0000 Platelet-activating factor (PAF) is an important chemical mediator in the field of inflammation, but its function in the skin is unclear. To unravel the role of PAF, we focused on lysophosphatidylcholine acyltransferase 2 (LPCAT2 also called LPLAT9), a biosynthetic enzyme involved in PAF production, and investigated the role of PAF in allergic contact dermatitis (ACD) and irritant contact dermatitis (ICD). We measured the amount of PAF in the skin and investigated the ear swelling responses and leukocyte infiltration into the skin following the application of 2,4,6-trinitro-1-chlorobenzene (TNCB) or croton oil in wild-type (WT) and LPCAT2 knockout (LPCAT2-KO) mice. The amount of PAF was increased in the skin of WT mice after TNCB or croton oil application but not detected in LPCAT2-KO mice. The ear swelling response was decreased in LPCAT2-KO mice compared with that in WT mice. In the ACD model, the numbers of lymphocytes, eosinophils, macrophages, mast cells and neutrophils were smaller in LPCAT2-KO mice than in WT mice. In the ICD model, the ear swelling response was also decreased in LPCAT2-KO mice compared with that in WT mice. When double staining of each inflammatory cell type and LPCAT2 was performed in ACD tissue, marked co-staining of the eosinophil marker and LPCAT2 was observed. In addition, LPCAT2 expression was observed in the epidermis. These results indicate that PAF is involved in the infiltration of several cell types into the sites of allergic and non-allergic skin inflammation. Furthermore, eosinophils and keratinocytes are primarily responsible for PAF production in skin inflammation.\u0000 </div>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 11","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disabled 2 (Dab2) Regulates Tumour Progression in Skin Squamous Cell Carcinoma 禁用 2 (Dab2) 调节皮肤鳞状细胞癌的肿瘤进展。

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2024-11-06 DOI: 10.1111/exd.70009

Sayoni Roy, Darshan Mehta, Suruchi Sawant, Sanjeev K. Waghmare

{"title":"Disabled 2 (Dab2) Regulates Tumour Progression in Skin Squamous Cell Carcinoma","authors":"Sayoni Roy, Darshan Mehta, Suruchi Sawant, Sanjeev K. Waghmare","doi":"10.1111/exd.70009","DOIUrl":"10.1111/exd.70009","url":null,"abstract":"Dab2 is an endocytic adaptor protein involved in various physiological processes and signaling pathways. Dab2 is deregulated in various cancers; however, its role in skin squamous cell carcinoma ( SCC) has not been elucidated yet. In the present study, we used the DMBA/TPA induced murine skin carcinogenesis model to examine the role of Dab2 in skin tumour progression. We generated tamoxifen inducible Dab2 conditional knockout system for our study. Loss of Dab2 led to delayed papilloma initiation and reduced papilloma burden. Delayed papilloma initiation was due to reduce proliferative potential of the papillomas due to Dab2 loss. Furthermore, while the WT papillomas progressed to SCC, the papillomas formed in Dab2 cKO mice failed to undergo malignant conversion to SCC. Dab2 cKO tumours showed reduced expression of K8, a marker for aggressive tumour. Moreover, Dab2 ckO tumours failed to undergo EMT as shown by reduced expression of Vimentin and Twist1. Dab2 cKO tumours also showed reduced expression of Sox2, a stem cell marker. Furthermore, qPCR analysis showed upregulation of Dab2 expression in the human skin cancer cell lines as compared to normal human skin keratinocytes. In patients, TCGA data analysis of skin cancer melanoma (SKCM) showed a trend where high levels of Dab2 correlated with poor overall survival. The present study shows that Dab2 promotes tumour progression in skin SCC.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 11","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurofibromatosis Type 1 Patients With Epilepsy: A Comprehensive Analysis of Demographics, Comorbidities and Healthcare Outcomes 神经纤维瘤病 1 型癫痫患者：人口统计学、并发症和医疗结果的综合分析。

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2024-11-06 DOI: 10.1111/exd.70011

Nilesh Kodali, Audrey Terrany, Keshav D. Kumar, Shae Chambers, Shivkar Amara, Robert A. Schwartz

{"title":"Neurofibromatosis Type 1 Patients With Epilepsy: A Comprehensive Analysis of Demographics, Comorbidities and Healthcare Outcomes","authors":"Nilesh Kodali, Audrey Terrany, Keshav D. Kumar, Shae Chambers, Shivkar Amara, Robert A. Schwartz","doi":"10.1111/exd.70011","DOIUrl":"10.1111/exd.70011","url":null,"abstract":"Neurofibromatosis Type 1 (NF1) is the most common neurocutaneous disorder in the United States. Due to a nonfunctional mutation in the NF1 gene, the disorder may initially present with café-au-lait spots and later numerous neurofibromas across the body. Epilepsy is a rare comorbidity of NF1, with an incidence of just 4%–7%. While abnormal electroencephalograms have been seen in up to 25% of NF1 patients, very few studies have investigated the association between epilepsy and the NF1 patient profile. This study was aimed at evaluating the associations between epilepsy and demographics, comorbidities and healthcare outcomes in NF1 patients. The 2017 National Inpatient Sample (NIS) database was retrospectively queried for patients with NF1 using ICD-10 PCS codes. Chi-square tests were used in univariable analysis to compare patients within this cohort with and without epilepsy. Regression analysis was used in multivariable analysis to identify comorbidities that were predictors of epilepsy and the effect of comorbidities on outcomes. 4635 patients with NF1 were identified, of which 655 (14.1%) had epilepsy and 3980 (85.9%) did not. The NF1 patient population with epilepsy was largely comprised of White males of lower household income in larger urban/teaching hospitals and who used Medicare or Medicaid. Multivariable logistic regression revealed that malignant brain neoplasms, paralytic ileus, scoliosis, pregnancy complications, paralysis, other neurological disorders, metastatic cancer, coagulopathy, drug abuse and hypertension were predictors of developing epilepsy in NF1 patients. Additionally, epilepsy in NF1 patients was associated with a shorter length of stay, lower total charges and fewer total procedures.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 11","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incontinence-Associated Dermatitis-Like Skin Changes Induced by the Application of Absorbent Pads Containing Bacteria and Artificial Urine in Rats 使用含细菌和人造尿的吸收垫诱发大鼠失禁相关皮炎样皮肤变化

IF 3.5 3区医学

Experimental Dermatology Pub Date : 2024-11-05 DOI: 10.1111/exd.70013

Ai Horinouchi, Yuko Mugita, Sanai Tomida, Chihiro Takizawa, Daijiro Haba, Hiromi Sanada, Gojiro Nakagami

{"title":"Incontinence-Associated Dermatitis-Like Skin Changes Induced by the Application of Absorbent Pads Containing Bacteria and Artificial Urine in Rats","authors":"Ai Horinouchi, Yuko Mugita, Sanai Tomida, Chihiro Takizawa, Daijiro Haba, Hiromi Sanada, Gojiro Nakagami","doi":"10.1111/exd.70013","DOIUrl":"https://doi.org/10.1111/exd.70013","url":null,"abstract":"Incontinence-associated dermatitis (IAD) is one of the most serious complications in older people with incontinence. Controlling urine property in absorbent pads could be effective for preventing IAD caused by bacterial urine. However, no animal model has been established to evaluate their effectiveness. This study aimed to induce IAD-like skin changes using absorbent pads containing bacterial urine and to confirm their pathophysiology in rats. Hairless Wistar Yagi rats were divided into the bacteria-containing urine (BU) and the bacteria-free urine (U) groups. A 10-h-attachment of absorbent pads containing artificial urine with/without bacteria to the skin pretreated with sodium lauryl sulfate was performed repeatedly for 5 days. Macroscopic findings and skin barrier function were examined every day, and histological changes, inflammatory responses and bacterial quantification in tissue samples were examined on Day 5. The BU group exhibited significant skin redness from Day 3, significant elevation of transepidermal water loss from Day 1, and histological changes, including significantly thickened epidermis, abnormal keratinocyte differentiation and erythrocyte leakage. Inflammation, confirmed by higher myeloperoxidase-positive cells, elevated tumour necrosis factor-α expression, and vascular endothelial damage, indicated by CD31 and pentraxin 3-positive cells, were observed in the BU group. The bacterial quantification showed no significant difference between the groups. IAD-like skin changes including histological changes and inflammation were suggested to be caused by urine properties altered by bacteria. This study proposed a new animal model for evaluating the effectiveness of absorbent pads in controlling the urine properties of bacterial urine on preventing IAD.","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":"33 11","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.70013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142579732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0