European Surgical Research最新文献

{"title":"Bilateral Pudendal Thigh Flap In The Creation of A Neovagina and Restoration of Utero-Neovaginal Continuity – A Case Report","authors":"K. Ogundipe, Elizabeth Evelyn Enoch","doi":"10.33425/2689-1093.1040","DOIUrl":"https://doi.org/10.33425/2689-1093.1040","url":null,"abstract":"The pudendal thigh flap is a sensate flap based on the posterior labial artery, which has shown great versatility in the reconstruction of genital anomalies as well as genital oncologic reconstruction. This report showcases the creation of a neovagina with the restoration of uterovaginal continuity in a 12-year-old girl who had vaginal atresia. She presented with concealed menstruation and cyclical abdominal pain and had a creation of a neovagina with utero-vaginal continuity using a bilateral pudendal thigh fascio-cutaneous flap. Post-operatively, she attained normal menstrual flow and a sensate neovagina with a satisfactory vaginal canal depth. Partial wound dehiscence of the flap donor site was managed with dressing and healing by secondary intention. Even in new centres, the pudendal thigh flap remains a versatile flap for managing vaginal atresia.","PeriodicalId":12222,"journal":{"name":"European Surgical Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84346816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality of Information in YouTube Videos on Penis Enlargement Surgery","authors":"Perçin Karakol, Tuncay Taş","doi":"10.33425/2689-1093.1039","DOIUrl":"https://doi.org/10.33425/2689-1093.1039","url":null,"abstract":"Background: An increasing number of patients and their aesthetic curiosity are causing the online questioning of penis enlargement surgery (PES). Although there is a wealth of healthcare information about PES on the internet, the quality of PES-related information on YouTube, the most visited online video streaming service, has not fully known. It is aimed to evaluate the quality of information about PES in YouTube videos. Materials and Methods: In January 2021, we cross-sectionally performed YouTube search using the keywords \"penis enlargement surgery\", \"penis lengthening surgery\", or \"penis thickening surgery\". We sorted the videos as reliability in terms of containing scientifically proven information by 1 urologist and 1 plastic surgeon. Irrelevant, non-Turkish, and silent videos were not included. Video demographics were analyzed based on the quality and source of the video. A 5-point global quality scale, a 5-point reliability (DISCERN) tool, kappa statistic, the intraclass correlation coefficient, and descriptive statistics in the form of proportions and percentages were used. Results: A total of one-hundred PES videos published on YouTube between 2015-2021 were searched. Thirty-nine of these videos were removed due to duplicate, irrelevant, lack of sound or misleading information. Deceptive videos had a significantly higher number of likes than useful videos. On the other hand, useful videos had significantly more views than misleading videos. Useful videos had a higher DISCERN score compared to misleading videos. The fact that the uploader was sourced by professionals and doctors working at universities significantly contributed to the usefulness of the videos. Conclusion: As searching for information about PES on YouTube, patients are exposed to low-quality and confusing videos. Complexity in the number of views and likes could make accessing the informative PES video difficult. It may be recommended that patients prefer PES videos created by professionals.","PeriodicalId":12222,"journal":{"name":"European Surgical Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88468050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front & Back Matter","authors":"","doi":"10.1159/000525322","DOIUrl":"https://doi.org/10.1159/000525322","url":null,"abstract":"","PeriodicalId":12222,"journal":{"name":"European Surgical Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65301517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"139th Congress of the German Society of Surgery (DGCH)","authors":"","doi":"10.1159/000523908","DOIUrl":"https://doi.org/10.1159/000523908","url":null,"abstract":"","PeriodicalId":12222,"journal":{"name":"European Surgical Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44094649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"139th Congress of the German Society of Surgery (DGCH)","authors":"F. Hansen, A. Mittelstädt, Anne Jacobsen, M. Podolska, Dina Kouhestani, Izabela J. Swierzy, Lotta Roßdeutsch, M. Brunner, C. Krautz, R. Gruetzmann, Alan Bénard, G. Weber, Guanxi Wang, Franziska Langner, R. Jackstadt, F. Greten, C. Geppert, C. Günther, M. Waldner, M. Stürzl, E. Naschberger","doi":"10.1159/000523882","DOIUrl":"https://doi.org/10.1159/000523882","url":null,"abstract":"Background: Barrett’s esophagus (BE), the result of chronic exposure of esophageal mucosa to acid exposure, predisposes to the development of esophageal adenocarcinoma (EAC). While the incidence of EAC is rapidly increasing, its prognosis remains poor, with a five-year survival rate of 20%. For advanced cancer stages, chemotherapy together with surgery is considered to be the standard in therapy. Consequently, the discovery of new molecular targets for an efficient characterization of EAC patients is necessary. CKAP4 has been attributed to be increased in various cancer entities and has a role in tumorigenesis. However, its role in EAC development and progression is widely unknown. Materials and Methods: In a Barrett’s esophagus, in vitro cell culture model of BE and EAC, CKAP4 expression levels were verified with qPCR and western blot. In OE33 and OE19 cells a knockdown of CKAP4-expression was done by siRNA for 48h and 72h. In siCKAP4 transfected cells FACS (apoptosis), western blot analysis, colony forming assays, spheroid formation and proliferation assays were performed. Using western blot, the impact of CKAP4-knockdown on the phosphorylation of Akt, MAP-Kinase, GSK3ß and ß-Catenin investigated. Furthermore, Ki67- and p21-mRNA-expression was analyzed in siCKAP4 cells using qPCR. Results: CKAP4 was present in all cell lines of the Barrett’s sequence (squamous epithelium, metaplasia, dysplasia CKAP4 OE19 OE33 Background: Adhesion molecules play a crucial role in tumor growth and metastasis. Histone deacetylase (HDAC) are responsible for epigenetic modifications even in cancer cells. Whether the inhibition of HDACs has an effect in the metastasis and intercellular Background: The HLA-genotype of a person defines the repertoire of peptides that can be presented to T cells. We aimed to determine if HLA-I homozygosity could translate in a smaller repertoire of tumour neoantigens potentially predisposing such individuals with a disadvantage to fight a nascent tumour. Materials and Methods: High-resolution HLA-genotyping combined with cancer EGA and nonsynonymous called used to predict high and moderate affinity binders the Epitope Data Base” and gene were performed Results: Frequency of HLA homozygosity in EGA patients Abstracts our Background: Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease with one of the lowest 5-year survival rates of all solid tumors. The determination of prognostic factors with PDAC is essential for predicting the outcomes and for identifying appropriate treatment strategies. Lymph node 8a is located above the pancreas head and was shown to have prognostic value for predicting severity in PDAC patients. The characteristic feature of lymph node 8a is the presence of abundant leucocytes representing both lymphoid and myeloid lineages. One major cellular compart-ment of lymph nodes are CD20+ B cells, which have been shown to be involved in the tumorigenesis of solid tumors. In this study, we sought to phenotype B","PeriodicalId":12222,"journal":{"name":"European Surgical Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46516380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front & Back Matter","authors":"Xiaoling Zhang Shanghai, D. Gouma, J. Habermann, M. Heger, J. Kalff","doi":"10.1159/000523934","DOIUrl":"https://doi.org/10.1159/000523934","url":null,"abstract":"","PeriodicalId":12222,"journal":{"name":"European Surgical Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41834245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potentials of CCL21 and CBS as Therapeutic Approaches for Breast Cancer.","authors":"Jing Peng,&nbsp;Danhua Zhang","doi":"10.1159/000521671","DOIUrl":"https://doi.org/10.1159/000521671","url":null,"abstract":"<p><strong>Objective: </strong>The present research set out to ascertain the roles of CC chemokine ligand 21 (CCL21) and cystathionine beta-synthase (CBS) in breast cancer (BC) cell biological behaviors and the relationship of CCL21 and CBS expression with the clinicopathological features of patients with BC.</p><p><strong>Methods: </strong>Immunohistochemistry of CCL21 or CBS was performed in 18 intraductal cancer tissues, 124 invasive BC tissues, 50 paraneoplastic tissues, 50 lobular hyperplasia tissues, and 30 normal breast tissues. For cell experiments, two human BC cell lines (MDA-MB-231 and MCF-7) and a human breast epithelial cell line (MCF-10A) were utilized to detect the expression of CCL21 and CBS. After loss- and gain-of-function assays for CCL21 or CBS, the expression of CBS and CCL21 was measured by quantitative real-time polymerase chain reaction and western blot. Additionally, BC cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and 5-ethynyl-2'-deoxyuridine staining, and BC cell migration was determined by scratch test and Transwell assay.</p><p><strong>Results: </strong>In the clinical data, the positive rate of CCL21 or CBS was significantly higher in invasive BC tissues than in intraductal BC tissues, lobular hyperplasia tissues, paraneoplastic tissues, and normal breast tissues (p < 0.05 or p < 0.01). CBS or CCL21 expression shared close association with the clinicopathological characteristics and the poor prognosis of BC patients. In cell experiments, overexpression of CCL21 or CBS enhanced the proliferative and migratory abilities of BC cells.</p><p><strong>Conclusion: </strong>CCL21 and CBS promoted BC cell migration and proliferation. CCL21 or CBS expression was strongly related to the poor prognosis of BC patients.</p>","PeriodicalId":12222,"journal":{"name":"European Surgical Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9133334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Oral Pirfenidone on Colon Anastomosis Healing and Adhesion Formation in Rats.","authors":"Shadya Betancourt-Vicencio,&nbsp;Manuel Rodrigo Prieto-Aldape,&nbsp;Andrea Socorro Alvarez-Villaseñor,&nbsp;Clotilde Fuentes-Orozco,&nbsp;Ana Olivia Cortes-Flores,&nbsp;Guadalupe Castillo-Cardiel,&nbsp;José Antonio Sánchez-Martínez,&nbsp;Emilio Alberto Reyes-Elizalde,&nbsp;Paola Guadalupe González-Hernández,&nbsp;José Gerardo Zamora-Inzunza,&nbsp;Victoria Amaranta Romero-Arredondo,&nbsp;José Francisco Barbosa-Camacho,&nbsp;Irma Valeria Brancaccio-Pérez,&nbsp;Bertha Georgina Guzmán-Ramírez,&nbsp;Alejandro González-Ojeda","doi":"10.1159/000523711","DOIUrl":"https://doi.org/10.1159/000523711","url":null,"abstract":"<p><strong>Introduction: </strong>Many experimental studies have examined multiple drugs or treatments to improve the healing of intestinal anastomoses. Synthetic prostacyclin analogs, immunosuppressants, erythropoietin, growth hormone, insulin-like growth factor type 1, synthetic metalloproteinases inhibitors, and hyperbaric oxygen therapy have produced promising results in low-risk models of anastomosis dehiscence. However, in high-risk models, only hyperbaric oxygen therapy has been shown to be useful. Pirfenidone (PFD), a commonly used antifibrosing drug, has not been shown to be effective for this purpose. Our objective was to evaluate the effects of PFD on anastomosis healing and adhesion genesis in a low-risk rat model of dehiscence of colonic anastomosis.</p><p><strong>Methods: </strong>An experimental study was conducted on 40 healthy Wistar rats randomly assigned to the control group or PFD experimental group (20 rats in each group). Colon anastomosis was performed 3 cm above the peritoneal reflection using the same technique in all animals. Mechanical resistance was studied by measuring bursting pressure. Adhesions were evaluated macroscopic and histologically using common staining techniques. Animals received the first PFD dose 12 h after surgery at a dose of 500 mg/kg one a day (SID) for 5 consecutive days. On day 6, the animals were reoperated on to measure the bursting pressure in situ and to classify adhesions macroscopically, and the anastomosed colon was resected for histological analysis.</p><p><strong>Results: </strong>There were no deaths, complications, or anastomosis dehiscence in either group. The mean bursting pressure was 120.8 ± 11 mm Hg and 135.5 ± 12.4 in the control and PFD groups, respectively (p < 0.001). The adhesions were less dense and had less inflammatory cell infiltration in the PFD group (p < 0.02 and 0.002, respectively). Collagen content was slightly higher in the PFD group (p = 0.04).</p><p><strong>Conclusions: </strong>Our results revealed favorable effects of PFD in this low-risk colon anastomosis model; for example, the bursting pressure was higher, and the macroscopic adhesions were soft and exhibited less inflammatory infiltration and higher collagen content in the PFD group than in the control group. The results showing that PFD treatment was associated with better healing of minor adhesions seem to be paradoxical because the therapeutic indications for this drug are directed at treating fibrosing diseases.</p>","PeriodicalId":12222,"journal":{"name":"European Surgical Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9871618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cell Therapies for Gastrointestinal Anastomotic Healing: A Systematic Review and Meta-Analysis on Results from Animal Studies.","authors":"Apostolos Gaitanidis,&nbsp;Leonidas Kandilogiannakis,&nbsp;Eirini Filidou,&nbsp;Alexandra Tsaroucha,&nbsp;George Kolios,&nbsp;Michail Pitiakoudis","doi":"10.1159/000526603","DOIUrl":"https://doi.org/10.1159/000526603","url":null,"abstract":"<p><strong>Background: </strong>Despite considerable progress in surgical techniques, anastomotic leak (AL) is a common complication after gastrointestinal surgery. Stem cells are a promising therapy to improve healing and have been used in gastrointestinal anastomoses. In this study, we perform a systematic review and meta-analysis to evaluate the efficacy of stem cell therapies in preventing ALs among animal studies.</p><p><strong>Methods: </strong>A systematic review of the literature was performed by searching PubMed, Web of Science, and the Cochrane Library. We considered all anastomoses of the gastrointestinal tract (excl. biliary) from the esophagus to the rectum. Outcomes included AL rates on postoperative day (POD) 7 and the latest time point reported.</p><p><strong>Results: </strong>Fourteen studies were identified, evaluating stem cells in gastrointestinal anastomoses, of which 1 was on esophageal, 2 on gastric, 2 on small intestinal, and 9 on colorectal anastomoses. Meta-analysis did not show significant differences in AL rates on POD 7 (odds ratio [OR] 0.34, 95% confidence interval [CI]: 0.04-3.15, p = 0.248, I2 = 34.1%, 95% CI: 0-75.2%, Q = 6.07, df = 4, p = 0.194), but there was a nonsignificant trend for lower AL rates at the latest time point reported (OR 0.28, 95% CI: 0.08-1.01, p = 0.052, I2 = 34%, 95% CI: 0-70.8%, Q = 10.6, df = 7, p = 0.157).</p><p><strong>Conclusion: </strong>Stem cell therapy may be associated with lower AL rates in gastrointestinal anastomoses, though meta-analysis is severely inhibited by heterogeneous study design. More studies are needed to determine the therapeutic potential of stem cells.</p>","PeriodicalId":12222,"journal":{"name":"European Surgical Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10629735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of Cannabinoid Type 2 Receptors in the Favorable Effects of Sumatriptan on the Random-Pattern Skin Flap Survival in Rats: A Novel Potential Target.","authors":"Armin Aryannejad,&nbsp;Nafise Noroozi,&nbsp;Seyed Mohammad Tavangar,&nbsp;Samira Ramezani,&nbsp;Amir Rashidian,&nbsp;Reza Laripour,&nbsp;Mojtaba Yousefi Zoshk,&nbsp;Ahmad Reza Dehpour,&nbsp;Mohsen Chamanara","doi":"10.1159/000521581","DOIUrl":"https://doi.org/10.1159/000521581","url":null,"abstract":"<p><strong>Introduction: </strong>Recent investigations have indicated the potential therapeutic role of cannabinoid type 2 (CB2) receptors in various inflammatory-related disorders. However, the role of these receptors has not been studied in skin flap models to date. In this study, we aimed to evaluate the possible involvement of CB2 receptors in the anti-inflammatory effects of sumatriptan, improving the random-pattern skin flap survival in rats.</p><p><strong>Methods: </strong>In a controlled experimental study, 36 male Wistar rats were randomly divided into 6 study groups (n = 6 per group). Two doses of sumatriptan (0.1 and 0.3 mg/kg) were administered intraperitoneally 30 min before harvesting the flap tissue. In a separate group, SR144528 (a selective CB2 receptor inverse agonist) was injected before the most effective dose of sumatriptan to determine the possible involvement of CB2 receptors in its action. Histopathological examinations, the expression level of CB2 receptors (Western blot analysis), and IL-1β and TNF-α concentrations (ELISA) were explored in the skin flap sampled tissues.</p><p><strong>Results: </strong>Sumatriptan 0.3 mg/kg remarkably enhanced the skin flap survival in all macroscopic and microscopic investigations compared to the control group (p < 0.001). IL-1β and TNF-α levels were significantly attenuated (p < 0.001), and the expression of CB2 receptors in skin cells was amplified in rats treated with sumatriptan 0.3 mg/kg (p < 0.05) compared to the control group. However, the administration of SR144528 (2 mg/kg) nullified all the protective effects of sumatriptan 0.3 mg/kg.</p><p><strong>Conclusion: </strong>We discovered that CB2 receptors play a crucial role in the favorable effects of sumatriptan on skin flap survival as a novel mechanism of action. So, targeting these receptors seems to be a dependable method in skin flap surgeries to ensure its survival and prevent tissue necrosis. Further experimental and clinical investigations are needed to ensure the safe clinical application of this method.</p>","PeriodicalId":12222,"journal":{"name":"European Surgical Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10632112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}