FEBS Open Bio最新文献_第7页

A minimal cellulosome-like system in Cellulosilyticum lentocellum. lentocellum中最小的纤维素样系统。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-08-19 DOI: 10.1002/2211-5463.70330

John Allan, Amias Alstrom-Moore, Gary W Black

{"title":"A minimal cellulosome-like system in Cellulosilyticum lentocellum.","authors":"John Allan, Amias Alstrom-Moore, Gary W Black","doi":"10.1002/2211-5463.70330","DOIUrl":"https://doi.org/10.1002/2211-5463.70330","url":null,"abstract":"Cellulosomes are efficient enzymatic nanomachines which have arisen for the degradation of cellulosic biomass. They are found abundantly in soil-dwelling microbes and bacteria which thrive in the stomachs of ruminant mammals. Two protein domains, cohesins and dockerins, characterise cellulosomes. These domains interact with each other to form, in many cases, enormous complexes with as many as 160 individual proteins. However, genome annotation of Cellulosilyticum lentocellum DSM 5427 revealed a single cohesin domain (encoded by Clole_2599) and a single dockerin domain (Clole_2598). Therefore, we recombinantly expressed ClcC and ClcD and found they form a (predicted ~ 104 kDa) heterodimeric complex. We show that this complex formation enhances cellulase activity approximately 2-fold on insoluble microcrystalline cellulose and 1.25-fold on soluble carboxymethyl cellulose. Moreover, we identified two additional candidate interacting partners for ClcC, one of which appears to bind via a non-canonical interface. These findings suggest that cellulosomal principles can operate in highly reduced cohesin-dockerin systems.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13487519/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148790106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Threonine 348 regulates the subcellular localization of PTEN. 苏氨酸348调节PTEN的亚细胞定位。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-08-19 DOI: 10.1002/2211-5463.70328

Takashi Kato, Suzu Tanaka, Miyu Ohashi

{"title":"Threonine 348 regulates the subcellular localization of PTEN.","authors":"Takashi Kato, Suzu Tanaka, Miyu Ohashi","doi":"10.1002/2211-5463.70328","DOIUrl":"https://doi.org/10.1002/2211-5463.70328","url":null,"abstract":"The tumor suppressor PTEN (phosphatase and tensin homolog) dephosphorylates PIP3 (phosphatidylinositol (3,4,5)-trisphosphate) at the plasma membrane and protects genomic integrity in the nucleus; thus, regulation of PTEN subcellular localization is crucial. Previous studies have shown that the PTEN350 fragment is markedly enriched in the nucleus, a feature not explained by the N-terminal nuclear localization signal. In this study, we generated PTEN fragments of various lengths and identified PTEN348 (residues 1-348), which showed prominent nuclear localization. Furthermore, the replacement of threonine 348 (Thr348) with other amino acids reduced the nuclear localization of the PTEN348 and PTEN350 fragments. Moreover, we found that PTENA4 and PTENK13R,A4 localized predominantly to the nucleus and plasma membrane, respectively, and that substitution of Thr348 with aspartic acid resulted in cytoplasmic localization in both mutants. Collectively, these results indicate that Thr348 is a key contributor to the regulation of PTEN subcellular localization.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13487556/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148790073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GelMA-based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma. 基于gelma的三维球体概括了髓系肉瘤的转录组学和功能特征。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-08-13 DOI: 10.1002/2211-5463.70321

Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti

{"title":"GelMA-based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma.","authors":"Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti","doi":"10.1002/2211-5463.70321","DOIUrl":"10.1002/2211-5463.70321","url":null,"abstract":"Myeloid sarcoma (MS) is a rare extramedullary manifestation of acute myeloid leukemia (AML). Conventional two-dimensional cultures fail to replicate the constraints of the MS microenvironment. We demonstrated that 5% gelatin methacrylate (GelMA) hydrogels provide a tunable three-dimensional matrix for in vitro modeling of MS. GelMA hydrogels exhibited tissue-like stiffness and oxygen diffusion properties, supporting the formation of viable spheroids from myeloid leukemia cell lines. The 3D context induced G1 cell cycle arrest and apoptotic progression, reflecting dormancy at extramedullary sites. RNA sequencing revealed transcriptional reprogramming in GelMA-embedded spheroids, with an enrichment in ECM remodeling and metabolic pathways. Furthermore, transcriptomic profiles from 3D-cultured cells aligned with patient-derived MS samples, validating the model's relevance to MS. Thus, we present a GelMA-based system which provides a clinically relevant platform for investigating MS biology and screening potential therapeutic strategies.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13470959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148720866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U-87 MG cells. 胶质母细胞瘤来源的胶质瘤干细胞和U-87 MG细胞中表达的降钙素受体亚型的鉴定和表征。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-08-13 DOI: 10.1002/2211-5463.70322

Pragya Gupta, Sebastian Gb Furness, Tahereh Gharbi, Ric De Paoli-Iseppi, Shweta S Joshi, Michael Clark, David L Hare, Peter Wookey

{"title":"Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U-87 MG cells.","authors":"Pragya Gupta, Sebastian Gb Furness, Tahereh Gharbi, Ric De Paoli-Iseppi, Shweta S Joshi, Michael Clark, David L Hare, Peter Wookey","doi":"10.1002/2211-5463.70322","DOIUrl":"10.1002/2211-5463.70322","url":null,"abstract":"Glioblastoma (GBM) is a highly lethal brain cancer in which the calcitonin receptor (CT Receptor), encoded by the CALCR gene, is expressed in 78-88% of patient biopsies. Here, we investigate whether the CT Receptor plays a role in cancer cell survival. In cancer cell lines, knockdown of CALCR disrupts the cell cycle and induces apoptosis, supporting an essential pro-survival role. The CALCR gene produces three main transcripts in humans, of which Transcript 1 encodes CALCRb mRNA including exon 10 and is translated into the CTb Receptor isoform, and Transcripts 2 and 3 which are translated into the CTa Receptor. CALCRb expression is conserved across a diverse range of mammalian species. We examined the expression of all CT Receptor isoforms (CALCRtotal) and CALCRb expression in four high-grade glioma stem-like cell lines and in U-87 MG glioblastoma cells. Using qPCR, we observed stable levels of both CALCRtotal and CALCRb expression under conditions of autophagy or apoptosis, consistent with a requirement for CALCRb in cell survival. As alternative splicing (AS) of key genes in cancers confers tumour resilience, we investigated AS of CALCR transcript 2 using long-read nanopore sequencing. Unexpectedly, we discovered a novel AS event causing inclusion of exon 10 within Transcript 2 in all glioblastoma cell lines investigated. This finding, together with stable CALCRb expression under cellular stress and the finding by other groups confirming that knockdown of CT Receptor compromises cell survival, implicates the CTb Receptor as a potential oncoprotein.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13470297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148720191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using cell-free RNA to identify B- and T-cell clonality for diagnosis and monitoring of B- and T-cell neoplasms. 利用无细胞RNA鉴定B细胞和t细胞克隆，诊断和监测B细胞和t细胞肿瘤。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-08-13 DOI: 10.1002/2211-5463.70323

Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar

{"title":"Using cell-free RNA to identify B- and T-cell clonality for diagnosis and monitoring of B- and T-cell neoplasms.","authors":"Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar","doi":"10.1002/2211-5463.70323","DOIUrl":"10.1002/2211-5463.70323","url":null,"abstract":"Cell-free RNA (cfRNA) is emerging as a supplemental approach for liquid biopsy (LBx). Given that lymphoid cells and plasma cells express substantial quantities of immunoglobulin (Ig) and T-cell receptor (TCR) RNA, we investigated the utility of using cfRNA for detecting B-cell and T-cell clonality in LBxs. Using next-generation sequencing (NGS) of cfRNA, we clonotyped Igs and TCRs in LBx samples from patients with B-cell neoplasm, T-cell neoplasm, myeloid neoplasm and solid tumors alongside cancer-free individuals. To establish a clonality cutoff, we clonotyped tissue RNA from patients with confirmed clonal B- or T-cell and from polyclonal. Clonotype-naïve testing of LBx samples demonstrated B-cell clonality in 36% of B-cell neoplasms, 7% of normal, 8% of T-cell lymphomas, 13% of myeloid neoplasms, and 14% of solid tumors. T-cell clonality using TCR beta or gamma demonstrated T-cell clonality in 22% of T-cell neoplasms, 3% of normal, 5% of myeloid neoplasms, 7% of solid tumors and 3% of B-cell neoplasms. This confirms that Clonotype-naïve cfRNA testing in LBx is a reliable approach for detecting B- and T-cell clonality.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13470955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148720203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accurate and noninvasive prostate cancer detection using plasma-derived extracellular vesicle RNA. 利用血浆来源的细胞外囊泡RNA准确、无创地检测前列腺癌。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-08-07 DOI: 10.1002/2211-5463.70305

Hanping Wei, Haoran Wu, Wei Feng

{"title":"Accurate and noninvasive prostate cancer detection using plasma-derived extracellular vesicle RNA.","authors":"Hanping Wei, Haoran Wu, Wei Feng","doi":"10.1002/2211-5463.70305","DOIUrl":"10.1002/2211-5463.70305","url":null,"abstract":"Prostate cancer (PCa) is the most commonly diagnosed noncutaneous malignancy in men and a leading cause of cancer-related death worldwide. Its clinical heterogeneity, ranging from indolent to aggressive disease, presents major diagnostic and therapeutic challenges. However, prostate-specific antigen (PSA), the current standard biomarker, lacks sufficient sensitivity and specificity, resulting in both overdiagnosis and missed cases. Therefore, more accurate noninvasive biomarkers are urgently needed. In this study, we isolated plasma-derived extracellular vesicles (EVs) using a wheat germ agglutinin (WGA)-conjugated magnetic bead method and identified differentially expressed transcripts between PCa patient and healthy control (HC) EVs. Candidate biomarkers were selected through weighted gene co-expression network analysis (WGCNA) and Random Forest modeling, followed by validation using RT-qPCR in independent cohorts including PCa, benign prostatic hyperplasia (BPH), and HC samples. We identified three EV-derived RNAs (NM_024955, NR_047469, and NR_002564), which were significantly dysregulated in PCa compared with both HC and BPH. Combined analysis demonstrated high diagnostic performance, outperforming PSA and notably NM_024955 expression showed a strong correlation, indicating potential relevance to disease severity. These findings suggest that lectin-based EV isolation coupled with RNA profiling provides a robust and scalable platform for PCa diagnosis, offering promising noninvasive biomarkers to complement or improve current PSA-based screening.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148688761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying and characterising a plant GH1 β-glucosidase that exhibits hydrolytic activity on N-linked glucopyranoside. 鉴定和鉴定一种植物GH1 β-葡萄糖苷酶，该酶对n -链型葡萄糖苷具有水解活性。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-08-06 DOI: 10.1002/2211-5463.70309

Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner

引用次数: 0

Evolution-guided yeast complementation reveals functional differences in human PSPH variants. 进化引导酵母互补揭示了人类PSPH变异的功能差异。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-08-05 DOI: 10.1002/2211-5463.70308

Mauricio Campa-Álvarez, Diana Ascencio, Miguel Vallebueno-Estrada, Eduardo González-Orozco, Christian Eduardo Martínez-Guerrero, Rafael Montiel, Alexander DeLuna

{"title":"Evolution-guided yeast complementation reveals functional differences in human PSPH variants.","authors":"Mauricio Campa-Álvarez, Diana Ascencio, Miguel Vallebueno-Estrada, Eduardo González-Orozco, Christian Eduardo Martínez-Guerrero, Rafael Montiel, Alexander DeLuna","doi":"10.1002/2211-5463.70308","DOIUrl":"10.1002/2211-5463.70308","url":null,"abstract":"Deciphering how human genetic variants affect conserved metabolic enzymes is essential for understanding their evolutionary and clinical significance. Here, we combine sequence analyses of temporally stratified human genomes with a quantitative Saccharomyces cerevisiae complementation assay in a strain lacking SER2, the yeast gene required for the final step of L-serine biosynthesis, to examine functional differences among human phosphoserine phosphatase (PSPH) variants. Population-genomic comparisons between ancient hunter-gatherers and present-day humans identified two PSPH exons with elevated differences in nucleotide diversity, guiding the selection of two ancient-genome-prioritized variants (R27S and Q83H) for functional testing. To place their effects in functional context, we expressed each variant individually and compared complementation with the modern PSPH allele and two disease-associated alleles (D32N and A35T) across multiple environmental conditions. Human PSPH enhanced growth of the SER2 deletion mutant and revealed reproducible quantitative differences among alleles. The modern allele generally conferred the strongest complementation, while the ancient genome variants supported measurable but more condition-dependent rescue, and the disease-associated alleles showed the weakest complementation. These differences were broadly consistent across conditions, while specific environmental perturbations revealed context-dependent shifts in effect size. Together, our results establish a scalable framework that links evolutionary genomics with experimental functional assays to identify and evaluate human metabolic enzyme variants with measurable in vivo effects.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13439035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-513a promotes human erythroid differentiation by modulating c-Jun MiR-513a通过调节c-Jun促进人红细胞分化。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-08-03 Epub Date: 2026-03-08 DOI: 10.1002/2211-5463.70226

MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury

{"title":"MiR-513a promotes human erythroid differentiation by modulating c-Jun","authors":"MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury","doi":"10.1002/2211-5463.70226","DOIUrl":"10.1002/2211-5463.70226","url":null,"abstract":"Erythropoiesis is a highly coordinated process that generates mature red blood cells from hematopoietic stem-progenitor cells (HSPCs). Erythropoietin (EPO) is a major regulator of erythropoiesis and binds to the erythropoietin receptor (EPOR), leading to increased erythroid differentiation and proliferation. MicroRNAs (miRs) are important developmental regulators, and distinct miR expression patterns are associated with specific stages of hematopoietic differentiation. Expression profiling of EPO-stimulated human HSPCs revealed increased expression of miR-513a-5p in early erythroid cells. Enforced expression of miR-513a in primary human CD34+ HSPCs promoted erythroid differentiation, as determined by cell-surface marker expression and increased levels of erythroid molecules including hemoglobin. Similar results were observed in human TF-1 erythroleukemia cells, where miR-513a stimulated erythroid differentiation including increased GATA1 and hemoglobin expression and decreased GATA2 expression, even in the absence of EPO. Notably, miR-513a promoted erythroid differentiation in EPOR knockout (KO) TF-1 cells, but not in GATA1 KO TF-1 cells, indicating that miR-513a requires GATA1, but not EPOR, to stimulate erythropoiesis. Further analysis revealed that enforced expression of miR-513a was associated with reduced c-Jun and phospho-c-Jun protein levels. Overexpression of c-Jun inhibited both EPO- and miR-513a-stimulated erythropoiesis. Conversely, c-Jun KO TF-1 cells had increased hemoglobin protein expression, even in the absence of EPO, phenocopying miR-513a overexpression. In summary, this study identifies miR-513a as a positive regulator of early human erythropoiesis and supports a role for miR-513a in promoting erythroid differentiation by modulating c-Jun expression and increasing GATA1 expression.","PeriodicalId":12187,"journal":{"name":"FEBS Open Bio","volume":"16 8","pages":"1509-1526"},"PeriodicalIF":2.7,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13399029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147376436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana YlmG1仅定位于拟南芥叶绿体包膜，参与拟南芥前蛋白易位。

IF 2.7 4区生物学

FEBS Open Bio Pub Date : 2026-08-03 Epub Date: 2026-03-03 DOI: 10.1002/2211-5463.70222

Mengyi Li, Xueyang Zhao, Masato Nakai

引用次数: 0