Experimental eye research最新文献_第6页

A multiomic study of retinal tissues in mice with direct ocular exposure to vesicants 直接眼接触除湿剂小鼠视网膜组织的多组学研究

IF 3 2区医学

Experimental eye research Pub Date : 2025-05-14 DOI: 10.1016/j.exer.2025.110414

Assylbek Zhylkibayev , James Mobley , Mohammad Athar , Marina Gorbatyuk

{"title":"A multiomic study of retinal tissues in mice with direct ocular exposure to vesicants","authors":"Assylbek Zhylkibayev , James Mobley , Mohammad Athar , Marina Gorbatyuk","doi":"10.1016/j.exer.2025.110414","DOIUrl":"10.1016/j.exer.2025.110414","url":null,"abstract":"<div><div>This study employed a multiomic approach to investigate retinal tissue damage following direct ocular exposure (DOE) to vesicants (VSs)—namely, nitrogen mustard (NM) and lewisite (Lew). We explored both the acute and chronic stages of retinal injury by assessing functional, structural, and molecular changes. C57BL/6 mice were used to measure scotopic and photopic electroretinograms (ERGs) and to analyze TUNEL-positive retinal cells. Global retinal proteomics was conducted to identify common and unique signaling pathways. In addition, we performed targeted metabolomic and lipidomic analyses of retinal tissue to uncover significant metabolic changes. Our results demonstrated remarkable declines in ERG amplitudes at 2 and 4 weeks post-exposure, accompanied by an increase in TUNEL<sup>+</sup> retinal cells in response to DOE to both VSs. Our proteomic analysis revealed chronic oxidative stress, mitochondrial dysfunction, elevated RXR signaling, and increased levels of 28 proteins. Moreover, we observed a decline in the KEGG phototransduction pathways, along with the downregulation of photoreceptor-specific proteins, in response to both VSs. Consistent with the proteomic findings, targeted metabolomics identified a decline in phototransduction and steroid hormone biosynthesis, along with increases in D-amino acid and purine metabolism, as well as lysine degradation. These changes were associated with a GSSG/GSH ratio of 2.6, confirming the proteomic data on oxidative stress. Furthermore, lipidomic analysis revealed an increase in oxidative lipid levels, accompanied by a 3.4-fold increase in phosphatidylserine (PS), suggesting apoptotic cell death and a reduction in fatty acids (FAs). In conclusion, exposure to both VSs induced progressive retinal damage, altering major metabolic pathways and dysregulating lipid metabolism. Future studies should focus on identifying the responses of individual neuronal cell types to DOE to VSs to develop cell-specific countermeasures.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"257 ","pages":"Article 110414"},"PeriodicalIF":3.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying a role for oxytosis/ferroptosis in Pde6b-associated retinitis pigmentosa 在pde6b相关性视网膜色素变性中确定氧中毒/铁下垂的作用

IF 3 2区医学

Experimental eye research Pub Date : 2025-05-14 DOI: 10.1016/j.exer.2025.110424

Madison E. Weiss , Paola E. Parrales , Maumita Datta , Michelle Fleishaker , Galina Gvoriantchikova , Dmitry Ivanov , Abigail S. Hackam

{"title":"Identifying a role for oxytosis/ferroptosis in Pde6b-associated retinitis pigmentosa","authors":"Madison E. Weiss , Paola E. Parrales , Maumita Datta , Michelle Fleishaker , Galina Gvoriantchikova , Dmitry Ivanov , Abigail S. Hackam","doi":"10.1016/j.exer.2025.110424","DOIUrl":"10.1016/j.exer.2025.110424","url":null,"abstract":"<div><div>Inherited retinal diseases (IRDs) are a large heterogeneous group of diseases that lead to visual impairment and complete vision loss. Retinitis pigmentosa (RP) is an IRD with progressive degeneration of photoreceptors and has been associated with mutations in over 80 genes. In this study, we investigated the mechanism of retinal degeneration caused by an inherited mutation in the <em>Pde6b</em> gene in the <em>rd10</em> mouse model of RP, with a focus on alternative programmed cell death pathways. RNA-seq analysis was used to identify changes in gene expression in <em>rd10</em> mice, using C57BL/6J mice as non-degenerating genetic background controls. The functional role of differentially expressed genes was investigated using pharmacological treatments and visual acuity was assessed using optomotor kinetic tracking assay. We found increased expression of genes involved in inflammatory response, while expression of genes involved in photoreceptor function and homeostasis were decreased. We also demonstrated increased expression of genes that regulate oxytosis/ferroptosis, a type of regulated necrosis that can promote inflammatory responses. We found no significant changes in expression of genes controlling other types of regulated necrosis. Treating <em>rd10</em> mice with oxytosis/ferroptosis inhibitors led to significant improvements in visual acuity. Therefore, these findings suggest that disruption of <em>Pde6b</em> activity results in photoreceptor death via oxytosis/ferroptosis, contributing to inflammatory responses in the retina. Our results identify for the first time a possible role of oxytosis/ferroptosis in a model of inherited retinal degeneration and provide a foundation for further studies exploring oxytosis/ferroptosis inhibitors as a potential therapeutic strategy for RP.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"257 ","pages":"Article 110424"},"PeriodicalIF":3.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the link between Co-stimulatory gene polymorphisms and clinical manifestations in Graves' ophthalmopathy 探讨Graves眼病共刺激基因多态性与临床表现的关系。

IF 3 2区医学

Experimental eye research Pub Date : 2025-05-14 DOI: 10.1016/j.exer.2025.110423

Ding-Ping Chen , Chia-Rui Shen , Wei-Tzu Lin , Yen-Chang Chu

{"title":"Exploring the link between Co-stimulatory gene polymorphisms and clinical manifestations in Graves' ophthalmopathy","authors":"Ding-Ping Chen , Chia-Rui Shen , Wei-Tzu Lin , Yen-Chang Chu","doi":"10.1016/j.exer.2025.110423","DOIUrl":"10.1016/j.exer.2025.110423","url":null,"abstract":"<div><div>Graves' ophthalmopathy (GO) is an autoimmune disorder that affects orbital tissues in approximately 30 % of Graves' disease patients. Single nucleotide polymorphisms (SNPs), particularly in immune-related genes, play a crucial role in the development of GO. This study investigates the association between SNPs in co-stimulatory molecules and specific clinical characteristics of GO, including laterality, orbital pain, swelling, diplopia, exophthalmos, redness, and eyelid retraction. Forty-one patients newly diagnosed with GO were analyzed. Genomic DNA was extracted from their blood, and 98 SNPs were amplified using PCR and sequenced. Candidate SNPs, selected based on prior research, were analyzed using chi-square tests and genetic models to assess genotype and allele frequency differences related to clinical manifestations. CD28 SNPs rs3181096 (C vs. T: p = 0.001) and rs3181098 (G vs. A: p = 0.002) were found to show protective effects against eyelid inflammation, while the A-allele of rs200353921(p = 0.005) increased the risk of eyelid inflammation. PDCD1 SNPs rs36084323 (C vs. T: p = 0.004) and rs41386349 (G vs. A: p = 0.005) were linked to diplopia and eyelid inflammation, respectively. The T-allele of rs6705653 in PDCD1 was found to increase the risk of diplopia (p = 0.001) but decreased the risk of eyelid retraction (p = 0.002). Other SNPs, including rs2227982 (p = 0.003) and rs2227981 (G vs. A: p = 0.001), were also associated with diplopia and eyelid retraction, highlighting the complex genetic influences on the clinical manifestations of GO. Furthermore, interactions between age, gender, and SNPs were observed in relation to GO clinical features. These findings highlight the potential regulatory roles of these genes in influencing immune responses and orbital inflammation in GO. Understanding these genetic influences could help identify predictive markers and novel therapeutic targets for GO management.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"257 ","pages":"Article 110423"},"PeriodicalIF":3.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grx2 maintains GSH/GSSG homeostasis to enhance GPX4-mediated ferroptosis defense in UVB irradiation induced cataract Grx2维持GSH/GSSG稳态，增强gpx4介导的UVB照射致白内障铁上睑塌陷防御。

IF 3 2区医学

Experimental eye research Pub Date : 2025-05-12 DOI: 10.1016/j.exer.2025.110421

Chenjun Guo , Yong Guo , Jie Zhang , Jue Wang , Liping Su , Xiaona Ning , Xi Chen , Hong Yan

{"title":"Grx2 maintains GSH/GSSG homeostasis to enhance GPX4-mediated ferroptosis defense in UVB irradiation induced cataract","authors":"Chenjun Guo , Yong Guo , Jie Zhang , Jue Wang , Liping Su , Xiaona Ning , Xi Chen , Hong Yan","doi":"10.1016/j.exer.2025.110421","DOIUrl":"10.1016/j.exer.2025.110421","url":null,"abstract":"<div><h3>Purpose</h3><div>Ultraviolet B (UVB) irradiation induces cataract pathogenesis, and Glutaredoxin 2 (Grx2) deficiency causes the early onset of UVB-induced cataracts. Several researchers have shown that, apart from apoptosis and pyroptosis, UVB irradiation also can induce cell ferroptosis. We explored the role of ferroptosis caused by UVB irradiation in human lens epithelial cells (HLECs) and clarified how Grx2 protects against UVB-induced cataracts.</div></div><div><h3>Methods</h3><div>HLE-B3 cells and mice lenses were treated with DMSO or ferroptosis inhibitors after various doses of UVB irradiation. Cell morphology and ultrastructure were observed by optical microscope and transmission electron microscopy. Lens opacity was observed <em>ex vivo</em> using an optical microscope and <em>in vivo</em> using a slit lamp. The lipid peroxidation level was measured by C11-BODIPY probe and 4-HNE (the lipid peroxidation marker) protein expression. Cell viability was determined using the CCK-8 kit and propodium iodide (PI) immunofluorescence. Grx2 KO and KI mice, Grx2 silencing and Grx2 overexpression in HLE-B3 cell lines were used for <em>in vivo</em> and <em>in vitro</em> experiments respectively.</div></div><div><h3>Results</h3><div>UVB-caused HLE-B3 cells death, lens opacity and lipid peroxidation could be mitigated by ferroptosis inhibitors. Grx2 KO mice accelerate the appearance of lens opacity induced by UVB. Meanwhile, Grx2 silencing enhanced HLECs lipid peroxidation susceptibility, downregulated the GSH level, shrunk mitochondria, and reduced the number of cristae. Grx2 overexpression had opposite effects.</div></div><div><h3>Conclusions</h3><div>Ferroptosis appears involved in UVB-induced HLECs damage. Inhibiting ferroptosis prevented UVB-induced cataracts. Grx2 strengthens resistance to ferroptosis induced by UVB irradiation through maintaining HLEC cellular GSH/GSSG homeostasis.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"257 ","pages":"Article 110421"},"PeriodicalIF":3.0,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144076494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depletion of Polypyrimidine tract binding protein 1 (ptbp1) activates Müller glia-derived proliferation during zebrafish retina regeneration via modulation of the senescence secretome

IF 3 2区医学

Experimental eye research Pub Date : 2025-05-10 DOI: 10.1016/j.exer.2025.110420

Gregory J. Konar , Audrey L. Lingan , Kyle T. Vallone, Tu D. Nguyen, Zachary R. Flickinger, James G. Patton

引用次数: 0

A standardized in vivo protocol for ocular biodistribution of gold nanoparticles 金纳米颗粒眼部生物分布的标准化体内方案

IF 3 2区医学

Experimental eye research Pub Date : 2025-05-06 DOI: 10.1016/j.exer.2025.110409

Alexis Loiseau , Christelle Gross , Sylvain Guérin , Élodie Boisselier

{"title":"A standardized in vivo protocol for ocular biodistribution of gold nanoparticles","authors":"Alexis Loiseau , Christelle Gross , Sylvain Guérin , Élodie Boisselier","doi":"10.1016/j.exer.2025.110409","DOIUrl":"10.1016/j.exer.2025.110409","url":null,"abstract":"<div><div>Effective drug administration plays a pivotal role in the treatment of eye diseases. Each route of ocular administration has its advantages and limitations, but a common challenge is the low bioavailability of drugs at the target sites. New delivery nanosystems are required to ensure sufficient drug concentration over time at the target in order to improve therapeutic efficacy. Gold nanoparticles represent a promising strategy for improving drug delivery to the eye, but they can be difficult to track in biological systems. To optimize the formulations, it is crucial to understand the biodistribution profiles of nanoparticles in the eye. Designing, interpreting and compiling research on the ocular biodistribution of nanoparticles raise major challenges, particularly considering the various nanoparticle-based ocular delivery systems and the multiple available animal models. The <em>in vivo</em> spatiotemporal distribution of nanoparticles in the eye is generally measured at specific time points after animal euthanasia and eye collection. In this technical article, we propose a detailed standardization of <em>in vivo</em> protocols for ocular biodistribution studies of gold-based delivery systems in rabbits following topical application. The protocol covers all steps, including enucleation, eye dissection of various ocular tissues and their digestion, as well as <em>ex vivo</em> analysis of gold (Au) atom content from gold nanoparticles by inductively coupled plasma-mass spectrometry (ICP-MS) at specific time points.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"257 ","pages":"Article 110409"},"PeriodicalIF":3.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oleic acid association with primary angle-closure glaucoma: A finding using metabolomics 油酸与原发性闭角型青光眼的关系：代谢组学的发现

IF 3 2区医学

Experimental eye research Pub Date : 2025-05-04 DOI: 10.1016/j.exer.2025.110418

Xia Qi , Yunhai Dai , Xiaojing Pan , Xinmiao Shan , Qingshu Ge , Jinyan Zhou , Hongwei Wang , Jie Lan

{"title":"Oleic acid association with primary angle-closure glaucoma: A finding using metabolomics","authors":"Xia Qi , Yunhai Dai , Xiaojing Pan , Xinmiao Shan , Qingshu Ge , Jinyan Zhou , Hongwei Wang , Jie Lan","doi":"10.1016/j.exer.2025.110418","DOIUrl":"10.1016/j.exer.2025.110418","url":null,"abstract":"<div><div>Metabolomics has been used to study ophthalmic diseases for the past several years. However, there was rare metabolomics study was reported about primary angle-closure glaucoma (PACG). The purpose of this study was to explore a two-step metabolomics analysis method for identifying differential metabolites in the aqueous humor of patients with PACG. Aqueous humor samples were collected from 20 patients each with age-related cataracts, acute PACG, and chronic PACG. In the first step, the changed metabolites were identified using a non-targeted metabolic analysis. In the second step, metabolites showing consistent changes in both acute and chronic PACG groups were selected for targeted metabolic analysis. Further, correlation analysis was performed to study the correlation between differential metabolites and clinical data. The results demonstrated that 7495 metabolites were identified using non-targeted metabolomics methods; subsequently, seven consistently changed metabolites, including oxoadipic acid, heptanoic acid, oleic acid, quinolinic acid, 25-hydroxycholesterol, mannitol, and maleic acid, were selected in the PACG groups. Except for quinolinic acid, the other metabolites were identified using targeted metabolomics analysis. However, to avoid clinical drug bias, mannitol and maleic acid were excluded from further statistical analyses. The PACG groups showed a tendency towards higher oleic acid and oxoadipic acid contents, whereas only oleic acid was negatively correlated with sex and logarithm of the minimum angle of resolution vision. These findings not only indicate that oleic acid may be involved in the development of pathological changes in eyes with PACG but also provide a method for identifying differential metabolites for other clinical diseases.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"256 ","pages":"Article 110418"},"PeriodicalIF":3.0,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silencing METTL3 and YTHDF2 increases HSP70 expression and promotes corneal epithelial healing 沉默METTL3和YTHDF2可增加HSP70表达，促进角膜上皮愈合

IF 3 2区医学

Experimental eye research Pub Date : 2025-05-03 DOI: 10.1016/j.exer.2025.110419

Yapeng Jing , Jun Li , Yi Guan , Shulei Xing , Xuan Li

{"title":"Silencing METTL3 and YTHDF2 increases HSP70 expression and promotes corneal epithelial healing","authors":"Yapeng Jing , Jun Li , Yi Guan , Shulei Xing , Xuan Li","doi":"10.1016/j.exer.2025.110419","DOIUrl":"10.1016/j.exer.2025.110419","url":null,"abstract":"<div><div>Delayed healing of corneal epithelial injury can cause irreversible scarring and severely affect vision. Outstanding progress has been made in recent years regarding corneal epithelial repair. However, studies investigating the role of N6-methyladenosine (m6A) in this process remain limited. The main objective of this study was to explore the role of m6A modification and its regulators in the healing of the corneal epithelium. In this study, we used the C57BL/6 mouse corneal alkali burn model as an in vivo model and human corneal epithelial cells (HCECs) as an in vitro subject. Small interfering RNA (siRNA) was used to downregulate the expression of YTHDF2, METTL3, and FTO in HCECs. We evaluated the effects of downregulating m6A modification regulators on the proliferation, migration, and apoptosis of HCECs under basal conditions and following IL-1β stimulation. The results suggest that m6A and its regulators are involved in the healing process of the corneal epithelium. Following corneal alkali burns, m6A levels were elevated, while the expression of METTL3, FTO, and YTHDF2 was reduced. In HCECs stimulated with IL-1β, m6A levels were significantly increased, and the expression of FTO and YTHDF2 was decreased. Silencing METTL3 and YTHDF2 enhanced the proliferation and migration of HCECs by increasing HSP70 expression, thereby facilitating corneal epithelial healing. In contrast, silencing FTO may impede corneal epithelial healing by promoting apoptosis in HCECs and inhibiting their proliferation and migration. This study offers a novel perspective on the treatment of delayed corneal epithelial healing.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"256 ","pages":"Article 110419"},"PeriodicalIF":3.0,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative comparison of inner and outer retinal intraretinal hyperreflective foci in age-related macular degeneration 年龄相关性黄斑变性视网膜内、外视网膜内高反射灶的定量比较

IF 3 2区医学

Experimental eye research Pub Date : 2025-05-02 DOI: 10.1016/j.exer.2025.110415

Maryam Ashrafkhorasani , Rouzbeh Abbasgholizadeh , Abbas Habibi , Mehdi Emamverdi , Muneeswar Gupta Nittala , Mohammad Mahdi Aghayee , Charles C. Wykoff , SriniVas R. Sadda

{"title":"Quantitative comparison of inner and outer retinal intraretinal hyperreflective foci in age-related macular degeneration","authors":"Maryam Ashrafkhorasani , Rouzbeh Abbasgholizadeh , Abbas Habibi , Mehdi Emamverdi , Muneeswar Gupta Nittala , Mohammad Mahdi Aghayee , Charles C. Wykoff , SriniVas R. Sadda","doi":"10.1016/j.exer.2025.110415","DOIUrl":"10.1016/j.exer.2025.110415","url":null,"abstract":"<div><div>To quantitatively compare the spectral-domain optical coherence tomography (SD-OCT) characteristics of inner and outer intraretinal hyperreflective foci (IHRF) in eyes with age-related macular degeneration (AMD) and to investigate their potential pathophysiological implications.</div><div>This retrospective study included 64 eyes from 64 treatment-naive AMD patients. SD-OCT imaging (Spectralis, Heidelberg Engineering) was performed using a 20° × 20° macular scan centered on the fovea. Inner IHRF were defined as lesions located above the inner border of the inner nuclear layer, while outer IHRF were situated below the inner border of outer nuclear layer. Semi-automated segmentation and quantitative analysis of IHRF were performed using ImageJ. Parameters analyzed included mean area, normalized mean reflectivity, greatest linear dimension (GLD), and mean circularity.</div><div>A total of 32 eyes with 68 inner IHRF and 32 eyes with 113 outer IHRF were included. The mean area of inner and outer IHRF was 887.09 ± 942.63 μm<sup>2</sup> and 832.27 ± 881.75 μm<sup>2</sup>, respectively (p = 0.69). Normalized reflectivity was 1.18 ± 0.14 for inner IHRF versus 1.20 ± 0.17 for outer IHRF (p = 0.54), and circularity was 0.81 ± 0.19 for inner IHRF versus 0.79 ± 0.20 for outer IHRF (p = 0.71). The mean GLD measured 42.8 ± 35.2 μm for inner IHRF and 45.3 ± 30.4 μm for outer IHRF (mean difference: 2.5 μm, 95 % CI: 7.57 to 12.57; p = 0.595). No statistically significant differences were observed between inner and outer IHRF in any evaluated parameters.</div><div>Inner and outer IHRF in AMD share similar quantitative SD-OCT characteristics, suggesting common pathological mechanisms irrespective of retinal location. These findings challenge the notion of distinct cellular origins and highlight the need for further research.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"256 ","pages":"Article 110415"},"PeriodicalIF":3.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances on modeling retinal disease: Towards efficient gene/drug therapy 视网膜疾病建模的最新进展：迈向有效的基因/药物治疗

IF 3 2区医学

Experimental eye research Pub Date : 2025-05-02 DOI: 10.1016/j.exer.2025.110416

Elham Norouz Dolatabadi , Mohammad Reza Akbarzadeh Zaky , Fatima Hashim Abbas , Amir Eftekhari Milani , Helder André , Effat Alizadeh

引用次数: 0