Experimental eye research最新文献_第6页

Human embryonic stem cell-derived immunity-and-matrix-regulatory cells on collagen scaffold effectively treat rat corneal alkali burn. 胶原支架上的人类胚胎干细胞衍生免疫和基质调节细胞可有效治疗大鼠角膜碱烧伤。

IF 3 2区医学

Experimental eye research Pub Date : 2024-11-19 DOI: 10.1016/j.exer.2024.110164

Haimiao Lin, Baojie Guo, Zhongwen Li, Chenxin Wang, Wenyu Wu, Zhaoxiang Lu, Liu Wang, Jun Wu, Jinming Li, Jie Hao, Yun Feng

{"title":"Human embryonic stem cell-derived immunity-and-matrix-regulatory cells on collagen scaffold effectively treat rat corneal alkali burn.","authors":"Haimiao Lin, Baojie Guo, Zhongwen Li, Chenxin Wang, Wenyu Wu, Zhaoxiang Lu, Liu Wang, Jun Wu, Jinming Li, Jie Hao, Yun Feng","doi":"10.1016/j.exer.2024.110164","DOIUrl":"10.1016/j.exer.2024.110164","url":null,"abstract":"<p><p>Corneal alkali burns (CAB) are a severe form of ocular injury that often leads to significant vision loss, with limited effective treatment options available beyond corneal transplantation. Immunity and matrix-regulatory cells (IMRCs) have emerged as a promising alternative due to their ability to modulate immune responses and support tissue repair. This study investigates the efficacy of IMRCs on collagen scaffolds (IMRCs-col) for treating CAB in a rat model. We developed a novel treatment combining IMRCs with a collagen scaffold to align with the ocular surface structure. In vitro analyses showed that IMRCs-col significantly upregulated the expression of immune regulatory molecules, including IL-1RA and SCF. Additionally, IMRCs-col effectively inhibited the production of pro-inflammatory cytokines (IL-8 and Gro-a/CXCL1) while promoting pro-regenerative cytokines (bFGF, HGF, and PDGF). In an animal model of CAB, IMRCs-col transplantation demonstrated substantial efficacy in restoring corneal opacity and reducing neovascularization. Histological examination revealed reduced inflammation and improved corneal tissue regeneration compared to untreated CAB. Enhanced activation of pathways associated with anti-inflammatory responses and tissue repair was observed at days 3, 7, and 21 post-treatment.</p>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":" ","pages":"110164"},"PeriodicalIF":3.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical application of 666-15, a potent inhibitor of CREB, alleviates alkali-induced corneal neovascularization 局部应用 CREB 的强效抑制剂 666-15 可减轻碱引起的角膜新生血管。

IF 3 2区医学

Experimental eye research Pub Date : 2024-11-19 DOI: 10.1016/j.exer.2024.110165

Zuohong Li , Jianping Chen , Zhaohao Huang , Weifeng Huang , Kerui Wang , Xuanwei Liang , Wenru Su

{"title":"Topical application of 666-15, a potent inhibitor of CREB, alleviates alkali-induced corneal neovascularization","authors":"Zuohong Li , Jianping Chen , Zhaohao Huang , Weifeng Huang , Kerui Wang , Xuanwei Liang , Wenru Su","doi":"10.1016/j.exer.2024.110165","DOIUrl":"10.1016/j.exer.2024.110165","url":null,"abstract":"<div><div>Corneal neovascularization (CNV) is a dynamically regulated process that arises due to a disruption in the equilibrium between pro-angiogenic and anti-angiogenic factors. Various cytokines are released by vascular endothelial cells and macrophages in damaged cornea, ultimately inducing CNV. The cAMP-response element-binding protein (CREB), a nuclear transcription factor, potentially impacts tumor angiogenesis by modulating the secretion of angiogenic proteins. This study aimed to assess the impact of 666-15, a potent inhibitor of CREB, on angiogenesis using human microvascular retinal endothelial cells (HMRECs), RAW 264.7 macrophage cell line and alkali-induce CNV mouse model. In vivo, the topical application of 666-15 (0.05 mg/mL) to the alkali-burn corneas led to 45% reduction in CNV. Additionally, in vitro treatment with 666-15 is effective in suppressing the migration, proliferation, and tube formation by HMRECs. Furthermore, treatment with 666-15 resulted in a down-regulation of pro-angiogenic cytokines expression, including VEGF-A, TGF-β1, b-FGF, and MMP-2 but simultaneously increasing anti-angiogenic cytokines expression, such as ADAMTS-1, Thrombospondin-1 (Tsp-1) and Tsp-2, both in alkali-burn corneas and HMRECs. And 666-15 inhibited the recruitment and the cytokines expression (VEGF-A, MMP-2, IL-1β, TNF-α, MCP-1 and MIP-1) of macrophage. Our findings revealed that 666–15 may suppress the function of endothelial cells and angiogenesis by restoring the homeostasis of pro-angiogenic stimuli, suggesting its potential as a therapeutic agent in the treatment of CNV and other angiogenesis-driven diseases.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"250 ","pages":"Article 110165"},"PeriodicalIF":3.0,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of semaphorin7A in epithelial-mesenchymal transition and proliferative vitreoretinopathy semaphorin7A在上皮-间质转化和增殖性玻璃体视网膜病变中的作用

IF 3 2区医学

Experimental eye research Pub Date : 2024-11-18 DOI: 10.1016/j.exer.2024.110153

Shuang Song , Rufei Yang , Ying Su , Feng Wang

{"title":"Role of semaphorin7A in epithelial-mesenchymal transition and proliferative vitreoretinopathy","authors":"Shuang Song , Rufei Yang , Ying Su , Feng Wang","doi":"10.1016/j.exer.2024.110153","DOIUrl":"10.1016/j.exer.2024.110153","url":null,"abstract":"<div><div>Proliferative vitreoretinopathy (PVR) is a multifactorial ocular condition characterized by the development of fibrotic membranes inside the vitreous cavity and on the detached retina, which can result in severe blindness. Semaphorin7A (Sema7a) is involved in axon growth, inflammatory responses, and immune regulation; however, its role in PVR and regulatory mechanisms in retinal pigment epithelium (RPE) cells remains unclear. This study aimed to examine Sema7a in PVR and the underlying mechanisms. Transcriptome sequencing was used to investigate the changes in mRNA expression profiles. Western blotting, immunofluorescence, and real-time polymerase chain reaction (RT-PCR) were utilized to investigate the potential mechanism of Sema7a on epithelial-mesenchymal transition (EMT) in RPE cells. Stimulating RPE cells with transforming growth factor beta-1 (TGF-β1) decreased the levels of epithelial markers but increased those of mesenchymal markers. Based on transcriptome sequencing, many molecules associated with PVR progression were regulated. PVR vitreous fluid proteomics data analysis showed that Sema7a significantly changed at different levels. Silencing <em>Sema7a</em> in RPE cells attenuated TGF-β1-induced EMT and their ability to induce experimental PVR; in contrast, recombinant Sema7a (rSema7a) directly triggered EMT in RPE cells. TGF-β1 induction mechanically activated the PI3k-AKT and MAPK pathways, while Sema7a knockdown by short interfering RNA lowered the phosphorylation of the PI3k-AKT/MAPK signaling pathway. Therefore, Sema7a may be a viable therapeutic target for PVR due to its crucial role in the TGF-β1-induced EMT of RPE cells.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"250 ","pages":"Article 110153"},"PeriodicalIF":3.0,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of protein profile in vitreous samples of patients with epiretinal membrane by proteomic approaches 用蛋白质组学方法评估视网膜外膜患者玻璃体样本中的蛋白质概况

IF 3 2区医学

Experimental eye research Pub Date : 2024-11-17 DOI: 10.1016/j.exer.2024.110160

Fatma Sumer , Berna Ozkan , V. Levent Karabas , Gurler Akpinar , Murat Kasap

{"title":"Assessment of protein profile in vitreous samples of patients with epiretinal membrane by proteomic approaches","authors":"Fatma Sumer , Berna Ozkan , V. Levent Karabas , Gurler Akpinar , Murat Kasap","doi":"10.1016/j.exer.2024.110160","DOIUrl":"10.1016/j.exer.2024.110160","url":null,"abstract":"<div><div>This study aims to characterize idiopathic epiretinal membrane (iERM) using proteomic analysis to enhance diagnosis and treatment strategies. In a prospective case-control clinical trial, vitreous fluids (VF) from twelve iERM patients were collected during surgery and analyzed by 2DE-based MALDI TOF-TOF MS/MS. PANTHER and STRING analyses were performed to investigate the biological relationships between the identified proteins and to determine relevant cellular pathways. A total of 148 proteins were identified, including 24 that were unique to iERM. Grouping the proteins by biological processes revealed that most were involved in cell adhesion (n = 6), proteolysis (n = 10), and complement activation (n = 8). Compared to control VF, 12 proteins were upregulated and 12 downregulated in iERM VF, with the differentially expressed proteins strongly associated with inflammation. Proteomic analysis highlighted complement and inflammatory proteins as potential biomarkers or therapeutic targets for iERM. Given that inflammation and fibrosis play critical roles in iERM, further investigation into these differential proteins holds significant clinical relevance. Despite the challenge of recruiting suitable patients, we believe the results of this study provide a valuable foundation for future research.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"250 ","pages":"Article 110160"},"PeriodicalIF":3.0,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complement C3 knockout protects photoreceptors in the sodium iodate model 在碘酸钠模型中，补体 C3 基因敲除可保护光感受器。

IF 3 2区医学

Experimental eye research Pub Date : 2024-11-16 DOI: 10.1016/j.exer.2024.110161

Tan Wang , Ying Song , Brent A. Bell , Brandon D. Anderson , Timothy T. Lee , Weihong Yu , Joshua L. Dunaief

{"title":"Complement C3 knockout protects photoreceptors in the sodium iodate model","authors":"Tan Wang , Ying Song , Brent A. Bell , Brandon D. Anderson , Timothy T. Lee , Weihong Yu , Joshua L. Dunaief","doi":"10.1016/j.exer.2024.110161","DOIUrl":"10.1016/j.exer.2024.110161","url":null,"abstract":"<div><div>Complement factor 3 (C3) has emerged as a primary therapeutic target in age-related macular degeneration (AMD) supported by genetic, histologic, and clinical trial evidence. Yet, the site(s) of action are unclear. The purpose of this study was to test the effect of C3 knockout on photoreceptors and retinal pigment epithelial cells (RPE) in the sodium iodate (NaIO<sub>3</sub>) model, which mirrors some features of AMD. <em>C3</em><sup><em>−/−</em></sup> and WT mice, both on a C57Bl/6J background, were injected intraperitoneally with 25 mg/kg NaIO<sub>3</sub>. Electroretinography and optical coherence tomography were performed 7 days later to assess retinal function and structure, respectively. Then, mice were euthanized for retinal immunohistochemistry, quantitative real-time PCR and enzyme-linked immunosorbent assays. NaIO<sub>3</sub> increased C3 protein levels in the neural retina but not RPE. WT but not <em>C3</em><sup><em>−/−</em></sup> mice showed NaIO<sub>3</sub>-induced iC3b deposition on photoreceptor outer segments. <em>C3</em><sup><em>−/−</em></sup> mice were partially protected against photoreceptor layer thinning. There was partial preservation of rod and cone function in the <em>C3</em><sup><em>−/−</em></sup> group. Neither RPE structure nor function was protected. These results suggest outer segment opsonization contributes to photoreceptor death in this model, and that targeting C3 can protect photoreceptor structure and function when RPE cells are stressed.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"250 ","pages":"Article 110161"},"PeriodicalIF":3.0,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deferiprone protects photoreceptors by inhibiting ferroptosis after experimental retinal detachment 去铁酮通过抑制实验性视网膜剥离后的铁蛋白沉积来保护感光细胞。

IF 3 2区医学

Experimental eye research Pub Date : 2024-11-15 DOI: 10.1016/j.exer.2024.110156

Ziyang Ye , Yuanye Yan , Feiyu Jin, Jiazhen Jiang, Can Deng, Lisong Wang, Kai Dong

{"title":"Deferiprone protects photoreceptors by inhibiting ferroptosis after experimental retinal detachment","authors":"Ziyang Ye , Yuanye Yan , Feiyu Jin, Jiazhen Jiang, Can Deng, Lisong Wang, Kai Dong","doi":"10.1016/j.exer.2024.110156","DOIUrl":"10.1016/j.exer.2024.110156","url":null,"abstract":"<div><div>The detachment of the retinal neuroepithelium from the retinal pigment epithelium (RPE), often due to a retinal tear and subsequent subretinal fluid (SRF) accumulation, is a critical factor leading to photoreceptor cells (PR) death and permanent vision impairment in retinal detachment (RD) scenarios. Predicting postoperative visual recovery is challenging, even with surgical reattachment. Research has indicated that increased iron and transferrin (TF) saturation in the vitreous fluid (VF) correlates with poorer visual outcomes, suggesting a potential role for ferroptosis, a form of regulated cell death, in PR following RD. To explore this hypothesis, we analyzed the VF of RD patients for ferroptosis markers, revealing reduced levels of glutathione peroxidase 4 (GPX4), glutathione (GSH), and reduced nicotinamide adenine dinucleotide phosphate (NADPH), alongside elevated levels of Long-chain acyl-CoA synthetase 4(ACSL4), malondialdehyde (MDA), and ferrous iron. We then developed a mouse model to simulate RD and administered the iron chelator deferiprone (DFP) as a treatment. Our findings indicated that DFP mitigated ferroptosis in the retina, thereby preserving retinal architecture and function. Collectively, our study establishes the occurrence of ferroptosis in RD and demonstrates the therapeutic potential of DFP in protecting PR and treating RD.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"250 ","pages":"Article 110156"},"PeriodicalIF":3.0,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SN promote retinal pathological neovascularization through activation of EGFR, IR and IGF-1R SN 通过激活表皮生长因子受体、IR 和 IGF-1R 促进视网膜病理性新生血管形成。

IF 3 2区医学

Experimental eye research Pub Date : 2024-11-15 DOI: 10.1016/j.exer.2024.110158

Wen Deng , Kongqian Huang , Ling Cui , Zhijie Niu , Diyang Ke , Li Jiang , Ningning Tang , Haibin Zhong , Qianqian Lan , Fan Xu , Fen Tang

{"title":"SN promote retinal pathological neovascularization through activation of EGFR, IR and IGF-1R","authors":"Wen Deng , Kongqian Huang , Ling Cui , Zhijie Niu , Diyang Ke , Li Jiang , Ningning Tang , Haibin Zhong , Qianqian Lan , Fan Xu , Fen Tang","doi":"10.1016/j.exer.2024.110158","DOIUrl":"10.1016/j.exer.2024.110158","url":null,"abstract":"<div><div>Secretoneurin (SN) is a neuropeptide derived from secretogranin II (SgII), mainly are involved in neuroendocrine system. The present study is aimed to investigate the role of SN in retinal pathological neovascularization and physiological vasculature. In the study, we found the overexpression of SgII in retina of Oxygen-Induced Retinopathy (OIR) mouse model, and <em>SgII</em> knockdown could alleviate pathological retinal neovascularization in OIR. Conversely, <em>SgII</em> knockdown have no detectable effect in embryonic physiological vasculature. Experiments <em>in vitro</em> and <em>in vivo</em> further verified SN's angiogenic effect on the eye. In further, we identified that SN promoted angiogenesis via activation of Epidermal Growth Factor Receptor (EGFR), Insulin Receptor (IR), and Insulin-like Growth Factor 1 Receptor (IGF-1R), and followed by the phosphorylation of PI3K-AKT-mTOR signaling. In summarize, our study suggests that SN might be a postnatal angiogenic factor, which was critically involved in retinal pathological neovascularization, but not in embryonic retinal physiological vasculature. Moreover, we identified the receptors and the downstream signaling involved in SN induced retinal angiogenesis.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"250 ","pages":"Article 110158"},"PeriodicalIF":3.0,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monochromatic light effects on refractive error, cone cell density and retinoic acid signaling in dorsal and ventral retina in guinea pigs 单色光对豚鼠背侧和腹侧视网膜屈光不正、视锥细胞密度和视黄酸信号的影响

IF 3 2区医学

Experimental eye research Pub Date : 2024-11-14 DOI: 10.1016/j.exer.2024.110155

Leilei Zou , Cheng Fang , Hong Liu , Rui Liu , Jinhui Dai

{"title":"Monochromatic light effects on refractive error, cone cell density and retinoic acid signaling in dorsal and ventral retina in guinea pigs","authors":"Leilei Zou , Cheng Fang , Hong Liu , Rui Liu , Jinhui Dai","doi":"10.1016/j.exer.2024.110155","DOIUrl":"10.1016/j.exer.2024.110155","url":null,"abstract":"<div><div>Cone cells have been found to influence refractive states. This study investigated whether cone cells and retinal acid (RA) plays a role in refractive states under monochromatic lights. Guinea pigs were exposed to blue (BL), green (GL), or white light (WL), respectively, for 8 weeks. Refractive error (RE), cone cell density, RA, retinoic acid receptor-β (RAR-β), collagen-I expression, and scleral thickness in dorsal and ventral eyes were assessed. Eyes exposed to BL showed a slower shift from hyperopia to emmetropia, particularly in the ventral retina, where higher S-cone density was linked to greater remaining hyperopia. In contrast, GL exposure led to myopic shifts, notably in the dorsal retina, where increased M-cone density was associated with greater reductions in refractive error. BL exposure resulted in similar decreases in RA and retinoic acid receptor-β (RAR-β) expression in both dorsal and ventral regions, along with elevated scleral collagen-I and thicker sclera. In contrast, GL exposure increased RA and RAR-β levels, while reducing scleral collagen-I and thickness. GL-associated changes in RAR-β expression and scleral thinning were more pronounced in the dorsal retina compared to the ventral retina, despite similar RA levels in both regions. These findings suggested that RA may not contribute to the hyperopic shifts with increased S-cone cell density in BL. However, increased RA and RAR-β may be correlated with ocular growth in guinea pigs exposed to GL, it may underlie myopic shifts with increased M-cone cell density.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"249 ","pages":"Article 110155"},"PeriodicalIF":3.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood integrin- and cytokine-producing T cells are associated with stage and genetic risk score in age-related macular degeneration 血液整合素和产生细胞因子的 T 细胞与老年性黄斑变性的分期和遗传风险评分有关。

IF 3 2区医学

Experimental eye research Pub Date : 2024-11-14 DOI: 10.1016/j.exer.2024.110154

Rianne Rijken , Els M. Pameijer , Bram Gerritsen , Sanne Hiddingh , Marilette Stehouwer , Joke H. de Boer , Saskia M. Imhof , Redmer van Leeuwen , Jonas JW. Kuiper

{"title":"Blood integrin- and cytokine-producing T cells are associated with stage and genetic risk score in age-related macular degeneration","authors":"Rianne Rijken , Els M. Pameijer , Bram Gerritsen , Sanne Hiddingh , Marilette Stehouwer , Joke H. de Boer , Saskia M. Imhof , Redmer van Leeuwen , Jonas JW. Kuiper","doi":"10.1016/j.exer.2024.110154","DOIUrl":"10.1016/j.exer.2024.110154","url":null,"abstract":"<div><div>Age-related macular degeneration (AMD) remains a leading cause of vision loss in the geriatric population. There are age-related changes in peripheral blood leukocyte composition, but their significance for AMD remains unclear. We aimed to determine changes in immune cell populations in the blood of AMD patients. A standardized 31-parameter flow cytometry analysis was conducted on peripheral blood mononuclear cells from 59 patients with early and advanced AMD and 39 controls without AMD, all older than 65 years. Fundus photography and optical coherence tomography were used to classify disease stages and a custom genotype array was used to compute an AMD genetic risk score based on 52 AMD disease risk variants (GRS-52). A generalized linear regression model corrected for age, sex, and smoking status revealed that AMD patients showed decreased frequencies of CD4<sup>+</sup> T helper cell population expressing Integrin Alpha E (CD103) (<em>Padj = 0.019</em>). We further noted that early AMD was characterized by increased interleukin-4 (IL-4)-producing CD4<sup>+</sup> T helper cells (<em>Padj = 0.013; <0.001</em>), as well as IL-4-producing cytotoxic CD8<sup>+</sup> T cells (<em>Padj = 0.016; <0.001</em>). Reclassification of samples based on the GRS-52 revealed that IL-17-producing T cells decreased incrementally across GRS-52 categories. In AMD, alterations in peripheral blood leukocyte populations are associated with genetic risk score and disease stage and include specifically IL-4 and IL-17A cytokine-producing and CD103 integrin-expressing T cell populations.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"250 ","pages":"Article 110154"},"PeriodicalIF":3.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of a COL1A1 gene haplotype with pathologic myopia in a Northern Chinese Han population 中国北方汉族人群中 COL1A1 基因单倍型与病理性近视的关系

IF 3 2区医学

Experimental eye research Pub Date : 2024-11-13 DOI: 10.1016/j.exer.2024.110151

Guangqi An , Min Zhang , Wenna Gao , Fan Yang , Lin Li , Youmei Xu , Xuemin Jin , Liping Du

{"title":"Association of a COL1A1 gene haplotype with pathologic myopia in a Northern Chinese Han population","authors":"Guangqi An , Min Zhang , Wenna Gao , Fan Yang , Lin Li , Youmei Xu , Xuemin Jin , Liping Du","doi":"10.1016/j.exer.2024.110151","DOIUrl":"10.1016/j.exer.2024.110151","url":null,"abstract":"<div><div>To investigate the relationship between <em>COL1A1</em> variations and the susceptibility to pathologic myopia (PM) among the general population in Northern China, we included 525 PM patients and 1105 non-myopic controls. All PM patients underwent comprehensive ophthalmologic examinations. DNA was extracted from peripheral venous blood samples and genotyped using the MassArray System. Statistical analyses, including Hardy-Weinberg equilibrium, χ<sup>2</sup> test, and linkage disequilibrium analysis, were conducted to compare the genotypic and allelic distributions of SNPs between PM patients and controls. The results showed no significant differences in the genotypic and allelic distributions of rs2075555, rs2269336, and rs1107946 between the PM and control groups. However, haplotype analysis revealed that the G-G-C and T-C-A haplotypes are risk factors for PM (G-G-C: OR = 1.399, 95% CI = 1.206–1.623, <em>P</em> < 0.001, <em>Pc</em> < 0.001; T-C-A: OR = 1.248, 95% CI = 1.064–1.456, <em>P</em> = 0.007, <em>Pc</em> = 0.021). Although individual SNPs in COL1A1 were not significantly associated with PM, specific haplotypes (G-G-C and T-C-A) were identified as risk factors. This suggests a potential role of <em>COL1A1</em> in the development of PM.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"250 ","pages":"Article 110151"},"PeriodicalIF":3.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142617747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0