Experimental eye research最新文献

{"title":"Nrf2-HO1 signaling pathway-mediated axial elongation in lens-induced myopia in Guinea pigs through regulation of tight junctions in retinal pigment epithelial cells","authors":"He-Yan Li ,&nbsp;Li Dong ,&nbsp;Rui-Heng Zhang ,&nbsp;Wen-Da Zhou ,&nbsp;Hao-Tian Wu ,&nbsp;Xu-Han Shi ,&nbsp;Chu-Yao Yu ,&nbsp;Yi-Tong Li ,&nbsp;Jost B. Jonas ,&nbsp;Wen-Bin Wei","doi":"10.1016/j.exer.2025.110477","DOIUrl":"10.1016/j.exer.2025.110477","url":null,"abstract":"<div><div>The study aimed to explore the potential involvement of Nrf2-HO1 (nuclear factor erythroid 2-related factor 2 heme oxygenase-1) in the process of myopic axial elongation, which influenced the disruption of tight junctions in retinal pigment epithelium (RPE) cells. Guinea pigs aged 2–3 weeks underwent bilateral lens-induced myopization (LIM) and received weekly intraperitoneal injections of the heme oxygenase-1 (HO-1) activators Hemin (25 mg/kg and 50 mg/kg, respectively), or Quercetin (25 mg/kg and 50 mg/kg, respectively). The study additionally included normal control groups with or without LIM and with vehicle injections. Ocular biometry, optical coherence tomography, and high-throughput sequencing were performed. Eyes with LIM showed a significantly increased expression of the Nrf2-HO1 signal pathway. Eyes with Hemin injections demonstrated axial elongation in a Hemin-dose dependent manner. Eyes with LIM and Quercetin injections showed in a dose-dependent manner and inhibition of the Nrf2 overexpression and an attenuation of axial elongation, a disruption of RPE cell tight junctions, and retinal and choroidal thinning. Immunofluorescence revealed the RPE cell as the main location of the HO-1 activation. Transmission electron microscope showed that Hemin was associated with a disruption of RPE tight junctions with axial elongation. The overexpression of Nrf2-HO1 signal pathway may be involved in LIM development and RPE tight junction disruption. The potential anti-myopic therapeutic value of Quercetin may be further explored.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"258 ","pages":"Article 110477"},"PeriodicalIF":3.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-29a regulates scleral remodelling via TLR7/8-dependent inflammatory signalling in myopia","authors":"Lingfeng Lv ,&nbsp;Qing Shao ,&nbsp;Jibo Zhou ,&nbsp;Yi Zhu","doi":"10.1016/j.exer.2025.110474","DOIUrl":"10.1016/j.exer.2025.110474","url":null,"abstract":"<div><div>Myopia, especially high myopia, is a global health concern with a rising prevalence, yet its underlying mechanisms remain incompletely understood. MicroRNAs (miRNAs) have been implicated in the pathogenesis of myopia, potentially playing a critical role in its development. This study aimed to investigate the mechanism by which miR-29a mediates scleral remodelling through the activation of Toll-like receptors (TLRs). By using a form-deprivation myopia (FDM) guinea pig model, we combined fluorescence in situ hybridization (FISH) and qPCR to demonstrate that the expression of miR-29a and inflammatory cytokines (TNF-α and IL-6) was significantly upregulated in the sclera of myopic eyes, whereas TLR7/8 expression remained unchanged. In human scleral fibroblasts (HSFs), FISH and RNA immunoprecipitation (RIP) assays revealed that miR-29a directly interacts with TLR7 and TLR8, forming ligand‒receptor complexes. This interaction activated downstream inflammatory signalling, leading to upregulated expression of proinflammatory cytokines (TNF-α and IL-6), downregulated expression of anti-inflammatory IL-10, and suppression of COL1A1 expression. Inhibition of TLR7/8 signalling reversed these effects, restoring COL1A1 levels and attenuating inflammatory responses. Our findings reveal a novel mechanism by which miR-29a promotes scleral remodelling through TLR7/8-mediated inflammatory signalling, providing new insights into the pathogenesis of myopia and potential therapeutic targets for its prevention and treatment.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"258 ","pages":"Article 110474"},"PeriodicalIF":3.0,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144243559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of break schedules on digital eye strain symptoms and ocular accommodation during prolonged near work","authors":"Beatriz Redondo ,&nbsp;Raimundo Jiménez ,&nbsp;Jesús Vera ,&nbsp;Mark Rosenfield","doi":"10.1016/j.exer.2025.110463","DOIUrl":"10.1016/j.exer.2025.110463","url":null,"abstract":"<div><div>This study evaluated the effects of four different break schedules on symptoms and signs of Digital Eye Strain (DES) during a 40-min reading task. Twenty-four young adults participated in four experimental conditions, performed on four different days in random order: no break, one break at 20 min, one break every 10 min and self-paced breaks. Primary outcomes included visual symptoms reported after the reading task, accommodative lag and variability during the reading task, and the near work-induced transient myopia (NITM) assessed post-task (including initial NITM and its decay). Visual symptoms were evaluated using a 10-item questionnaire, while accommodative measures were obtained with a binocular open-field autorefractor (Grand Seiko WAM-5500). Participants reported higher “eye irritation or burning” in the no-break condition compared to the 3-break (p &lt; 0.001) and self-paced breaks conditions (p = 0.008). Eye strain was also greater in the no-break condition than in the 3-break (p = 0.04) and self-paced breaks conditions (p = 0.04). Accommodative variability showed significant effects for both break schedule (p = 0.03) and time period (p = 0.02), with greater variability observed in the no-break and 3-break conditions during the final time interval. NITM was higher in the no-break condition compared with the 3-break (p = 0.02) and self-paced breaks conditions (p = 0.02), while NITM decay was faster in the 3-break condition (p = 0.001). Accommodative lag did not differ significantly across conditions. These findings highlight the potential benefits of individualized and frequent breaks for managing DES during prolonged screen use.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"258 ","pages":"Article 110463"},"PeriodicalIF":3.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruption of the 12-hour ultradian rhythm in ocular surface microbiota of T2DM C57BL/6J mice","authors":"Xinwei Jiao ,&nbsp;Hongyu Li ,&nbsp;Ting Wang ,&nbsp;Hongchen Fu ,&nbsp;Shiwu Wang ,&nbsp;Hong Liu ,&nbsp;Lei Wang ,&nbsp;Xiuyun Li ,&nbsp;Aijun Deng ,&nbsp;Zhijie Li","doi":"10.1016/j.exer.2025.110472","DOIUrl":"10.1016/j.exer.2025.110472","url":null,"abstract":"<div><div>The ocular surface (OS) microbiota exhibits a 12-h ultradian rhythm in healthy mice, essential for ocular homeostasis, yet its regulation in type 2 diabetes mellitus (T2DM), associated with ocular surface diseases, remains unclear. This study investigated the temporal dynamics of the OS microbiota in control (NC) and T2DM C57BL/6J mice over a 24-h cycle (ZT0, ZT6, ZT12, ZT18) using 16S rRNA sequencing and JTK_CYCLE analysis of diversity indices, OTU composition, and family-level abundance. In NC mice, a robust 12-h rhythm with distinct temporal patterns was observed. In contrast, T2DM disrupted this rhythmicity, with diversity indices and OTU compositions lacking temporal structure, and family-level rhythms, including those of <em>Rhodocyclaceae</em> and <em>Pseudomonadaceae</em>, altered, often shifting toward 24-h patterns in selected taxa and diversity measures. PCoA confirmed significant temporal clustering in NC but not in T2DM. These findings suggest that T2DM disrupts the 12-h ultradian rhythm of the OS microbiota, inducing complex temporal dysregulation, potentially contributing to ocular pathology, and highlight the potential of chronotherapeutic strategies to restore microbial rhythmicity and alleviate T2DM-related complications.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"258 ","pages":"Article 110472"},"PeriodicalIF":3.0,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144223323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DAPL1 inhibits epithelial-mesenchymal transition of retinal pigment epithelial cells by regulating the TGF-β/MITF pathway","authors":"Yaqi You ,&nbsp;Youjia Liu ,&nbsp;Lijing Huang ,&nbsp;Wan-ni Lu ,&nbsp;Yingxin Zhang ,&nbsp;Tianyin Nie ,&nbsp;Meiyu Jing ,&nbsp;J. Fielding Hejtmancik ,&nbsp;Xingyi Li ,&nbsp;Ling Hou ,&nbsp;Xiaoyin Ma","doi":"10.1016/j.exer.2025.110473","DOIUrl":"10.1016/j.exer.2025.110473","url":null,"abstract":"<div><div>Epithelial-mesenchymal transition (EMT) of the retinal pigment epithelium (RPE) is a critical factor in the development of retinopathies, including proliferative vitreoretinopathy (PVR) and age-related macular degeneration (AMD), which are the leading causes of blindness worldwide. Deficiency in DAPL1 can induce RPE-EMT in vivo, and <em>Dapl1</em> knockout mice (<em>Dapl1 −/−</em>) are prone to PVR, while aged <em>Dapl1 −/−</em> mice display AMD-like pathological features. However, the molecular mechanisms through which DAPL1 regulates RPE-EMT remain largely unknown. Here, using <em>Dapl1 −/−</em> mice and DAPL1 knockdown or overexpression RPE cells, we show that DAPL1 inhibits RPE-EMT by regulating the TGF-β/MITF signaling pathway, a critical signaling pathway/transcription factor in RPE cells. Overexpression of DAPL1 inhibits TGF-β-induced RPE-EMT, while deletion of <em>Dapl1</em> in mice activates TGF-β signaling, decreases MITF expression, and promotes RPE-EMT under physiological or PVR pathological conditions. Gene therapy demonstrates that transgenic overexpression of MITF in <em>Dapl1 −/−</em> mice inhibits RPE-EMT in vivo and prevents retinal detachment-induced PVR pathological progress, offering hope for future treatment. Similarly, pharmacological therapy with Isoviolanthin, a flavonoid glycoside isolated from traditional medicinal herbs, inhibits TGF-β signaling and increases MITF expression in RPE cells in <em>Dapl1 −/−</em> mice, which then effectively rescues experimental PVR in <em>Dapl1 −/−</em> mice. These results suggest that DAPL1 regulates RPE-EMT at least partial through the TGF-β/MITF pathway and that targeting the TGF-β/MITF pathway could be a potential therapeutic strategy to treat <em>Dapl1</em> deficiency-induced RPE-EMT-related retinal diseases, instilling hope for the future of retinal disease treatment.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"258 ","pages":"Article 110473"},"PeriodicalIF":3.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrin αvβ6 mediates the effects of age-related decreases in matrix stiffness on Tenon's fibroblast activation and bleb scarring in glaucoma","authors":"Lemeng Feng ,&nbsp;Wei Xiong ,&nbsp;Jiayang Yin ,&nbsp;Dongshi Guan ,&nbsp;Hangyu Li ,&nbsp;Jiamin Cao ,&nbsp;Ziyi Zhu ,&nbsp;Wenhua Zhang ,&nbsp;Feng Zhang","doi":"10.1016/j.exer.2025.110459","DOIUrl":"10.1016/j.exer.2025.110459","url":null,"abstract":"<div><div>Glaucoma, including primary open-angle glaucoma (POAG) and primary angle-closure glaucoma, leads to optic nerve injury and visual field loss, often necessitating surgical intervention to lower intraocular pressure (IOP). Trabeculectomy, the most common glaucoma surgery, could fail due to excessive scarring of the filtering bleb, driven by the hyperproliferation of human Tenon's fibroblasts (HTFs). Herein, the impact of aging on matrix stiffness in Tenon's capsule tissue, the role of extracellular matrix (ECM) stiffness in the phenotypic transformation of HTFs, and the regulatory function of integrin alphavbeta6 (αvβ6) were investigated. Matrix stiffness in Tenon's capsule tissue is notably lower in elder glaucoma patients in comparison with younger ones, with reduced levels of α-SMA, collagen I, and integrin αvβ6. GFS (Glaucoma Filtration Surgery) models were established in young and old SD rats, and it was observed that older rats exhibited lower ECM stiffness, reduced fibrosis, and decreased integrin αvβ6 expression. HTFs from elderly glaucoma patients showed reduced ECM stiffness, decreased viability, impaired migration, and diminished fibrotic responses. When HTFs from young glaucoma patients were cultured on substrates of varying stiffness, it was found that stiffer substrates increased cell viability, migration, collagen synthesis, and fibrosis marker expression. Additionally, knocking down integrin αvβ6 in HTFs cultured on stiffer substrates resulted in decreased cell viability, impaired migration, reduced collagen synthesis, and lower fibrosis marker expression. <em>In vivo</em> knockdown of integrin αvβ6 effectively reduced ECM stiffness and fibrosis, thereby attenuating bleb scarring after GFS in young rats. Collectively, the aging-associated changes in ECM stiffness and integrin αvβ6 expression contribute to reduced fibrosis, potentially enhancing the success of trabeculectomy in elder patients.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"258 ","pages":"Article 110459"},"PeriodicalIF":3.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on “Human umbilical cord-derived mesenchymal stem/stromal cells via suprachoroidal injection: A novel approach for experimental uveitis treatment”","authors":"Sıdıka Gerçeker Demircan","doi":"10.1016/j.exer.2025.110454","DOIUrl":"10.1016/j.exer.2025.110454","url":null,"abstract":"","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"258 ","pages":"Article 110454"},"PeriodicalIF":3.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of proteomics and artificial intelligence-driven OCT biomarker analysis in central retinal vein occlusion","authors":"Lorenzo Ferro Desideri ,&nbsp;Kentaro Kojima ,&nbsp;Nina Eldridge ,&nbsp;Denise C. Zysset-Burri ,&nbsp;Marie Ørskov ,&nbsp;Henrik Vorum ,&nbsp;Bent Honoré ,&nbsp;Martin S. Zinkernagel ,&nbsp;Lasse Jørgensen Cehofski","doi":"10.1016/j.exer.2025.110462","DOIUrl":"10.1016/j.exer.2025.110462","url":null,"abstract":"<div><div>Retinal OCT biomarker analysis by artificial intelligence (AI) has not previously been integrated with proteomics. Here, we combined the two techniques to elucidate novel molecular mechanisms in central retinal vein occlusion (CRVO). Proteomic data on aqueous humor samples from patients with treatment naïve CRVO complicated by macular edema (n = 21) and an age-matched, healthy control group (n = 20) was obtained from an existing cohort. Swept-source OCT macular scans (Topcon) from CRVO patients were analysed by AI-driven OCT software (Discovery platform, RetinAI AG, Bern Switzerland) to quantify retinal thicknesses and OCT biomarkers, including intraretinal fluid (IRF), subretinal fluid (SRF), and the outer nuclear layer thickness (ONL). Correlation analysis between protein expression and OCT biomarkers was performed. Proteins involved in complement activation and immune responses exhibited positive correlations with IRF, notably complement component C8 beta chain (r = 0.63, p = 0.0020) and Ig lambda-6 chain C region (r = 0.59, p = 0.0050). Negative correlations with IRF were identified for phosphatidylethanolamine-binding protein 1 (r = −0.66, p = 0.0010) and chordin-like protein 1 (r = −0.61, p = 0.0030). SRF was linked with insulin-like growth factor-binding protein complex acid labile subunit (r = 0.55, p = 0.010) and with extracellular superoxide dismutase (r = −0.63, p = 0.0022). Retinal layer thickness, particularly ONL and total retinal thickness, was positively associated with fibrinogen beta chain (r = 0.58, p = 0.0060) and fibronectin (r = 0.51, p = 0.019). The integration of AI and proteomics allowed for linking biological processes with specific OCT biomarkers. The combined analysis identified complement activation, immune response, oxidative stress, and extracellular matrix remodelling as likely driving forces of macular edema secondary to CRVO.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"258 ","pages":"Article 110462"},"PeriodicalIF":3.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibiting effect of LIPUS on epithelial-mesenchymal transition in lens epithelial cells","authors":"Junfen Li ,&nbsp;Yiqing Zhou ,&nbsp;Sicheng He ,&nbsp;Wenjing Mao ,&nbsp;Xinbing Han ,&nbsp;Xinyi Zhang ,&nbsp;Yan Wang","doi":"10.1016/j.exer.2025.110450","DOIUrl":"10.1016/j.exer.2025.110450","url":null,"abstract":"<div><div>To investigate the effect of low-intensity pulsed ultrasound (LIPUS) on epithelial-mesenchymal transition (EMT) in lens epithelial cells. EMT was induced using high glucose (HG) in SRA01/04 cells. Optimal parameters for LIPUS irradiation were determined by cell counting kit-8 assays and flow cytometry. Cell morphology was assessed by light microscopy, while cell migration ability was analyzed by a wound healing assay. Levels of specific proteins and the relationship between autophagy and the cytoskeleton were examined by immunofluorescence (IF) staining and Western blot (WB). Cytoskeletal structures were visualized by phalloidin staining and autophagosomes were quantified by transmission electron microscopy. EMT was successfully induced by HG treatment. Compared to the model group, LIPUS irradiation resulted in a change in cell morphology from spindle to oval, a significant decrease in cell migration area, and an increase in E-cadherin and LC3B/LC3A levels. In contrast, α-SMA and SQSTM1/P62 levels decreased, the number of autophagosomes increased and F-actin levels decreased in the LIPUS group. SRA01/04 cells treated with LIPUS irradiation after autophagy inhibitors 3-MA and CQ showed increased cell migration area compared to the 3-MA/CQ group; LC3B/LC3A levels decreased; SQSTM1/P62 and F-actin levels increased in the LIPUS + 3-MA/CQ group compared to 3-MA/CQ treatment alone. Colocalization of the cytoskeletal marker Arpc2 with the autophagy marker SQSTM1/P62 was also observed. After treatment with the cytoskeletal inhibitor CK666+LIPUS combination therapy, SQSTM1/P62 levels increased while LC3B/LC3A levels decreased. LIPUS inhibited HG-induced EMT by restoring autophagy, which appears to be associated with cytoskeletal remodeling.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"258 ","pages":"Article 110450"},"PeriodicalIF":3.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of gene recombination pattern induced by Tg(Crx-cre)1Tfur mouse strain in visual system organs","authors":"C. Felgerolle ,&nbsp;E. Villalonga ,&nbsp;A. Attallah ,&nbsp;A. Menuet ,&nbsp;S. Briault ,&nbsp;M. Ardourel ,&nbsp;O. Perche","doi":"10.1016/j.exer.2025.110461","DOIUrl":"10.1016/j.exer.2025.110461","url":null,"abstract":"<div><div>Retinas are more and more considered in research, as a part of the Central Nervous System (CNS) sharing the same embryonic origin as brain, and thus displays similarities to it on many aspects. Thus, deciphering retinal processes may bring information on downstream central structures of the CNS in addition to understanding retina by itself. Therefore, access to suitable <em>in vivo</em> tools to create appropriate models to decipher retina-specific phenomena and their impacts on other tissues became a need. Some years ago, the new murine strain Tg(Crx-cre)1Tfur was published as a tool to induce retina-specific gene recombination, and literature reported its efficiency to induce a global gene recombination in all retinal layers and cell types at post-natal ages. However, to our knowledge, no study of the retina-specificity of Tg(<em>Crx-Cre</em>)-induced recombination had been published yet. Here we aimed to further investigate the gene-recombination pattern induced by Tg(<em>Crx-Cre</em>) among the structures of the visual system, to bring clear clues of the fair use that could be done of the Tg(<em>Crx-Cre</em>) murine strain. Our results showed that Tg(<em>Crx-Cre</em>) induced a total gene recombination in retinas but also scattered significantly brain structures. Therefore we advise that Tg(Crx-cre)1Tfur must be used in studies focusing strictly on retina and conclusions should be carefully drawn taking into account the non-retina-specificity of gene recombination.</div></div>","PeriodicalId":12177,"journal":{"name":"Experimental eye research","volume":"258 ","pages":"Article 110461"},"PeriodicalIF":3.0,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}