2006 IEEE International Symposium on MicroNanoMechanical and Human Science最新文献_第5页

Intelligent Actuators Realizing Snake-like Small Robot for Pipe Inspection 智能执行机构实现蛇形小型管道检测机器人

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-11-01 DOI: 10.1109/MHS.2006.320333

A. Kuwada, K. Tsujino, K. Suzumori, T. Kanda

引用次数: 42

Model System of Exocytotic Release in Mast Cells That Cause Allergic Responses 肥大细胞分泌释放引起过敏反应的模型系统

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-11-01 DOI: 10.1109/MHS.2006.320255

H. Sakiyama, S. Tadokoro, M. Sasai, N. Hirashima

{"title":"Model System of Exocytotic Release in Mast Cells That Cause Allergic Responses","authors":"H. Sakiyama, S. Tadokoro, M. Sasai, N. Hirashima","doi":"10.1109/MHS.2006.320255","DOIUrl":"https://doi.org/10.1109/MHS.2006.320255","url":null,"abstract":"Exocytosis is a key process to secrete bioactive substances from cells, such as neurotransimitters, hormones, and inflammatory mediators from immune cells. Mast cells are typical non-neuronal secretory cells and secrete inflammatory mediators such as histamine and cause allergic responses. It has been shown that SNARE (soluble N-ethyl maleimide-sensitive factor attachment protein receptor) proteins play an essential role in exocytotic release in both neuronal cells and non-neuronal secretory cells. To mimic the exocytotic processes in mast cells, we developed an artificial membrane fusion system using liposomes that contains SNARE proteins. To prepare liposomes that contain SNARE proteins, SNARE proteins expressed in E. coli were purified and reconstituted into liposomes by solubilization-reconstitution methods using octylglucoside as a detergent. The membrane fusion is monitored by FRET (fluorescent resonance energy transfer) between fluorescence labeled phospholipids in liposomes (NBD- and rhodamine- labeled phospholipids). Using this assay system, we found that SNAP23, syntaxin 3 and VAMP8 are involved in exocytotic release in mast cells","PeriodicalId":121329,"journal":{"name":"2006 IEEE International Symposium on MicroNanoMechanical and Human Science","volume":"SE-10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126574949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultraprecision Micro-Machining of Single Crystal Germanium by Applying Elliptical Vibratlon Cutting 椭圆振动切削单晶锗的超精密微加工

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-11-01 DOI: 10.1109/MHS.2006.320323

N. Suzuki, Z. Yan, R. Hino, E. Shamoto, Y. Hirahara, T. Masuda

{"title":"Ultraprecision Micro-Machining of Single Crystal Germanium by Applying Elliptical Vibratlon Cutting","authors":"N. Suzuki, Z. Yan, R. Hino, E. Shamoto, Y. Hirahara, T. Masuda","doi":"10.1109/MHS.2006.320323","DOIUrl":"https://doi.org/10.1109/MHS.2006.320323","url":null,"abstract":"'Ultrasonic elliptical vibration cutting' technology, which has been developed by the authors, is applied to ultraprecision machining of single crystal germanium in the present research. The germanium is an excellent material for infrared optical devices, whereas ultraprecision micro-machining of the germanium is difficult due to its brittleness. Micro grooving experiments are carried out on the (100), (110) and (111) surfaces of single crystal germanium substrates, and basic effects of the elliptical vibration on the ductile micro-machining are clarified as follows. Critical depth of cut to generate a completely fracture-free surface on the single crystal germanium is increased significantly in all crystal orientations on the (100) and (111) surfaces, and ultraprecision micro grooving is realized successfully at a large depth of cut of 1 mum by applying the elliptical vibration cutting. This improvement by the elliptical vibration cutting is considered to be caused by reduction of instantaneous uncut chip thickness and cutting force in each cycle of the elliptical vibration. When the rake angle becomes negative, influence of the crystal orientation on the critical depth of cut becomes negligible, while it is considerable at 0 degree. On the other hand, the rake angle does not have considerable influence on average values of the critical depth of cut between -20 to 0 degree. The critical depth of cut is increased in all crystal orientations as the cutting speed is reduced in the elliptical vibration cutting","PeriodicalId":121329,"journal":{"name":"2006 IEEE International Symposium on MicroNanoMechanical and Human Science","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124514679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Surface modification of DMFC using ultra-low energy electron beam irradiation system with silicon base membrane 超低能电子束辐照硅基膜DMFC表面改性研究

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-11-01 DOI: 10.1109/MHS.2006.320260

M. Yamaguchi, Y. Yamada, J. Murase, Y. Goto, Y. Nakano, S. Hayase, M. Shikida, K. Sato

引用次数: 3

Multi-Scale Genetics towards Understanding the Hierarchy of Transcription Factor Network in Genome Regulation 基因组调控中转录因子网络层次的多尺度遗传学研究

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-11-01 DOI: 10.1109/MHS.2006.320221

A. Ishihama, H. Ogasawara, T. Shimada, J. Teramoto, A. Hasegawa, Y. Umezawa, K. Yabuki, Y. lshida, T. Inaba, M. Matsui, Y. Kitai, A. Kori, K. Yamada, K. Hirao, K. Yamamoto

{"title":"Multi-Scale Genetics towards Understanding the Hierarchy of Transcription Factor Network in Genome Regulation","authors":"A. Ishihama, H. Ogasawara, T. Shimada, J. Teramoto, A. Hasegawa, Y. Umezawa, K. Yabuki, Y. lshida, T. Inaba, M. Matsui, Y. Kitai, A. Kori, K. Yamada, K. Hirao, K. Yamamoto","doi":"10.1109/MHS.2006.320221","DOIUrl":"https://doi.org/10.1109/MHS.2006.320221","url":null,"abstract":"The model bacterium, Escherichia coli, is able to survive under various stressful conditions in nature. From the complete genome sequence, about 4,400 genes are considered to exist on the genome. Selective and regulated expression of the genes is critical for bacterial adaptation to environment. Since the number of RNA polymerase, the enzyme responsible for transcription, is less than that of total genes, we proposed that the controlled distribution of RNA polymerase among 4,400 genes determines the genome expression pattern. In order to understand the molecular basis of genome regulation, we have been concerned with the analysis of all 300 transcription factors, which are involved in the functional modulation of RNA polymerase for switching the gene selectivity. For this purpose, we have performed the systematic search for target genes and promoters recognized by each transcription factor using the newly developed genomic SELEX system. Here we describe some results of this multi-scale genetics focusing on the protein-protein and protein-DNA interaction networks involved in the genome regulation. One hitherto undescribed finding is that the transcription factors form interplay networks, in which one transcription factor controls the expression of another factor and so on. The putative transcription factors with unidentified functions are often located on top of the hierarchy of transcription factor cascade. Such networks appear to be needed for stress response in nature","PeriodicalId":121329,"journal":{"name":"2006 IEEE International Symposium on MicroNanoMechanical and Human Science","volume":"125 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123154445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bundling Transition of F-actin f -肌动蛋白的成束转变

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-11-01 DOI: 10.1109/MHS.2006.320320

S. Watanabe, K. Yoshikawa

{"title":"Bundling Transition of F-actin","authors":"S. Watanabe, K. Yoshikawa","doi":"10.1109/MHS.2006.320320","DOIUrl":"https://doi.org/10.1109/MHS.2006.320320","url":null,"abstract":"Filament of F-actin is formed from a linear array of G-actin and exhibits the characteristics as a negatively charged stiff polyelectrolyte with the persistence length above 1 mum. In living cellular system, F-actins from thick bundles with the help of specific proteins as the mediator. It has been found that bundling of F-actin filaments is generated with various cationic species, such as Fe3+ , spermidine3+, cationic synthetic polypeptides. It has been regarded that these multivalent cations work as a paste to stick the like-charge filaments. In the past studies, the bundling phenomenon of F-actin has frequently been observed through the increase of the light scattering. There observations indicated the cooperative, continuous nature in the transition. In the present study, we performed the observation on the bundling phenomenon by use of fluorescent microscopy. We found that the bundling transition proceeds through the coexistence of single filaments and bundles, which corresponds to the continuous nature of the transition revealed from the measurement of the light scattering. From these results, it is concluded that the bundling transition is characterized as a first-order phase transition under the criterion of Landau's symmetry argument","PeriodicalId":121329,"journal":{"name":"2006 IEEE International Symposium on MicroNanoMechanical and Human Science","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125540128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Direct Observation of Transformation Process of Giant Liposome Induced by Reconstructed Cytoskeletons 重建细胞骨架诱导巨脂质体转化过程的直接观察

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-11-01 DOI: 10.1109/MHS.2006.320296

M. Honda, Y. Tanaka-Takiguchi, T. Inaba, H. Hotani, K. Takiguchi

引用次数: 0

Single-molecule Structural and Functional Analyses of Nuclear Pore Complex 核孔复合物的单分子结构与功能分析

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-11-01 DOI: 10.1109/MHS.2006.320314

S. Otsuka, H. Takahashi, S. Yoshimura

引用次数: 0

Fabrication of Gratings for an X-ray Talbot Interferometer x射线塔尔博特干涉仪光栅的制备

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-11-01 DOI: 10.1109/MHS.2006.320247

M. Tanaka, Y. Takeda, D. Noda, W. Yashiro, K. Okuda, A. Momose, T. Hattori

{"title":"Fabrication of Gratings for an X-ray Talbot Interferometer","authors":"M. Tanaka, Y. Takeda, D. Noda, W. Yashiro, K. Okuda, A. Momose, T. Hattori","doi":"10.1109/MHS.2006.320247","DOIUrl":"https://doi.org/10.1109/MHS.2006.320247","url":null,"abstract":"X-ray based imaging measurement has found applications in fields such as medical diagnosis and nondestructive inspection, having become one of essential technologies. However, its low sensitivity to biologically soft tissues limits scope of applications. Several methods have been developed for X-ray phase imaging. We proposed a method using an X-ray Talbot interferometer. It offers advantages; it is a very simple setup using two gratings; it has a potential of phase imaging with a compact laboratory X-ray source, but the fabrication of an amplitude X-ray grating is a difficult, because the structure of grating must have high aspect ratio. We used deep X-ray lithography and electroforming of Au in fabricating gratings. The period and height of the grating were 5.3mum and 31mum. We evaluated the gratings, successfully achieved a high visibility of 71%. With the gratings that had a period of 8 mum and a height of 29 mum, we conducted X-ray phase tomography of rabbit liver and a mouse tail. As a result, we successfully observed soft tissues and high-density tissues. The gratings, therefore, had sufficient performance to conduct phase tomography","PeriodicalId":121329,"journal":{"name":"2006 IEEE International Symposium on MicroNanoMechanical and Human Science","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134043329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Multi-Scale Biosensing Techniques for Cell Chips 细胞芯片的多尺度生物传感技术

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-11-01 DOI: 10.1109/MHS.2006.320243

M. Suzuki, Y. Iribe

引用次数: 7