2006 IEEE International Symposium on MicroNanoMechanical and Human Science最新文献

A Method for In-Situ Targeting and Harvesting of Cultured Cells Under a Microscope 一种在显微镜下原位定位和收获培养细胞的方法

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-12-01 DOI: 10.1109/MHS.2006.320229

H. Takamatsu, S. Uchida

引用次数: 2

Nanorobotics System Simulation in 3D Workspaces with Low Reynolds Number 低雷诺数三维工作空间纳米机器人系统仿真

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-12-01 DOI: 10.1109/MHS.2006.320269

A. Cavalcanti, Tad Hogg, B. Shirinzadeh

引用次数: 11

Spatial and temporal application of microfluidics to cells 微流体技术在细胞中的时空应用

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-12-01 DOI: 10.1109/MHS.2006.320281

A. Yamada, Y. Katanosaka, S. Mohri, K. Naruse

引用次数: 1

Compact Vision System on a Chip Application 片上紧凑视觉系统的应用

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-12-01 DOI: 10.1109/MHS.2006.320276

H. Uvet, T. Arai, K. Inoue, T. Takubo, S. Kunimatsu

{"title":"Compact Vision System on a Chip Application","authors":"H. Uvet, T. Arai, K. Inoue, T. Takubo, S. Kunimatsu","doi":"10.1109/MHS.2006.320276","DOIUrl":"https://doi.org/10.1109/MHS.2006.320276","url":null,"abstract":"This paper describes the research and development of the two compact vision systems for real-time cell detection and manipulation in the capillary in order to apply on the \"desktop bioplant\" project which will achieve sort of cell manipulation tasks such as positioning, cutting, sizing, and so on, automatically between different interconnected modules. The first prototype vision system with sufficient magnification and image resolution has been designed to be boarded onto the PDMS chip. Design schema and preliminary experimental results of first proposed system have been also given. A second system with a commercially available small size and light-weight microscope unit has also been prototyped to be integrated under microfluidic channel. The integration of two models with desktop bioplant could bring a good solution to automation of the cell manipulation including nuclear transportation. While the size and weight of the total systems are decreased to provide portability and compatibility, determining the position and management of an egg-cell in a capillary has vital importance for the automation of nuclear transportation in a capillary with using micro robotics","PeriodicalId":121329,"journal":{"name":"2006 IEEE International Symposium on MicroNanoMechanical and Human Science","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127400718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstitution of in vitro inactivation and reactivation systems of DnaA protein for the control of chromosomal replication initiation in Escherichia coli 控制大肠杆菌染色体复制起始的dna蛋白体外失活和再激活系统的重建

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-12-01 DOI: 10.1109/MHS.2006.320253

M. Su’etsugu, K. Fujimitsu, T. Katayama

{"title":"Reconstitution of in vitro inactivation and reactivation systems of DnaA protein for the control of chromosomal replication initiation in Escherichia coli","authors":"M. Su’etsugu, K. Fujimitsu, T. Katayama","doi":"10.1109/MHS.2006.320253","DOIUrl":"https://doi.org/10.1109/MHS.2006.320253","url":null,"abstract":"The Escherichia coli initiator protein, DnaA binds to the replicational origin of the chromosome (oriC) and initiates the replication in a manner depending on ATP binding. Replication of mini-chromosome (a plasmid bearing oriC) has been reconstituted in vitro using crude extracts or purified proteins. In these in vitro systems, only ATP-bound form of DnaA is active to initiate a series of initiation reactions which leads to loading of the DNA polymerase III holoenzyme (pol III HE) onto DNA for DNA synthesis. In a manner dependent on Hda protein and DNA loading of pol III HE, ATP bound on DnaA is hydrolyzed. The resultant ADP-bound form of DnaA is inactive for the initiation. This inactivation system called RIDA (regulatory inactivation of DnaA) ensures the replication initiation only once per cell cycle. We here describe recent progress on our system to reconstitute the inactivation of DnaA by RIDA and a novel system to regenerate active DnaA. The reconstitution of the Hda-dependent DnaA-ATP hydrolysis requires the DNA-loaded form of the sliding clamp. This complex is formed by loading of the beta subunit of pol III HE onto DNA during replication process. Hda protein forms a stable complex with the sliding clamp, and most likely has a direct contact to ATP bound on DnaA. The cellular ATP-DnaA level fluctuates during the cell cycle. Regeneration from ADP-DnaA to ATP-DnaA is observed in vivo before the next round of the replication. Recently we have found a specific DNA segment DARS (DnaA-reactivating sequences) that has an activity to release ADP bound on DnaA. ATP-DnaA is regenerated by nucleotide-exchange in the presence of DARS and the elevated concentrations of ATP. The resultant ATP-DnaA was active in the mini-chromosome replication reaction in vitro. Thus, DARS can functionally reactivate ADP-DnaA. The inactivation-reactivation cycle of DnaA might be reconstituted in vitro in a manner controled by RIDA and DARS","PeriodicalId":121329,"journal":{"name":"2006 IEEE International Symposium on MicroNanoMechanical and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130217488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expansion of protein biosynthesis system including nonnatural amino acids 扩展蛋白质生物合成系统，包括非天然氨基酸

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-12-01 DOI: 10.1109/MHS.2006.320251

T. Ohtsuki, Y. Doi, T. Manabe, M. Sisido

引用次数: 0

In Vivo Microvascular Oxygen Measurements by Phosphorescence Quenching Technique 用磷光猝灭技术测定体内微血管氧

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-12-01 DOI: 10.1109/MHS.2006.320286

M. Shibata, S. Ichioka

引用次数: 0

Fabrication of 3D Tissue-Like Structure Ussing Magnetite Nanoparticles and Magnetic Force 利用磁铁矿纳米颗粒和磁力制备三维类组织结构

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-12-01 DOI: 10.1109/MHS.2006.320291

A. Ito, K. Ino, K. Shimizu, H. Honda, M. Kamihira

{"title":"Fabrication of 3D Tissue-Like Structure Ussing Magnetite Nanoparticles and Magnetic Force","authors":"A. Ito, K. Ino, K. Shimizu, H. Honda, M. Kamihira","doi":"10.1109/MHS.2006.320291","DOIUrl":"https://doi.org/10.1109/MHS.2006.320291","url":null,"abstract":"Novel technologies to establish 3D tissue-like constructs are desired for tissue engineering. In the present study, magnetic force and magnetite nanoparticles were used to construct a layered mesenchymal stem cell (MSC) sheet, a layered cardiomyocyte sheet, and a layered fibroblast sheet involving capillaries. Magnetite cationic liposomes (MCLs) were taken up by the target cells. When a magnet was set under a tissue culture dish, magnetically labeled target cells were attracted and then adhered to form a layered cell sheet. MSC sheets were harvested and transplanted into the bone defect in cranium of nude rats, resulted in formation of new bones surrounded by osteoblast-like cells. For cardiomyocyte sheets, the immunofluorescence staining revealed the existence of gap junctions within the cardiomyocyte sheets. For fibroblast sheets, normal human dermal fibroblasts (NHDFs) sheets contained the major dermal extracellular matrix components. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NHDF sheets, resulted in tube-like formation of HUVECs. These results suggest that this novel use of magnetite nanoparticles and magnetic force, which we refer to as \"magnetic force-based tissue engineering (Mag-TE)\", offers a major advancement in tissue engineering","PeriodicalId":121329,"journal":{"name":"2006 IEEE International Symposium on MicroNanoMechanical and Human Science","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127001137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dynamic study of micro-domains on a phospholipid bilayer membrane 磷脂双层膜微结构域的动态研究

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-12-01 DOI: 10.1109/MHS.2006.320254

M. Ichikawa, Y. Shitamichi, H. Tashirot, Y. Kimura

引用次数: 1

Three-dimensional Microstructuring of PDMS by Two-photon Microstereolithography 双光子微立体光刻技术制备PDMS的三维微结构

2006 IEEE International Symposium on MicroNanoMechanical and Human Science Pub Date : 2006-12-01 DOI: 10.1109/MHS.2006.320261

T. Hasegawa, K. Oishi, S. Maruo

引用次数: 4