Food Additives & Contaminants: Part A最新文献

{"title":"Metabolism and pharmacokinetics of mebendazole in Japanese pufferfish (Takifugu rubripes)","authors":"Lei Men, Yuhan Zhang, Keke Li, Zhong-yin Li, Chunbin Li, Xueyuan Zhang, Xiaojie Gong, Linlin Fang","doi":"10.1080/19440049.2022.2052974","DOIUrl":"https://doi.org/10.1080/19440049.2022.2052974","url":null,"abstract":"Abstract As a typical and broad-spectrum benzimidazole, mebendazole (MBZ) has long been used in human and veterinary medicine to treat parasitic infestations, and is widely employed in the aquaculture of Japanese pufferfish (Takifugu rubripes). However, there have been no studies examining the pharmacokinetic characteristics of MBZ in Japanese pufferfish. Furthermore, the presence of MBZ and its metabolites in animal-derived raw food represents a notable safety concern. Here, we investigated the metabolism of MBZ using a UPLC-Q-TOF system. Additionally, we evaluated the pharmacokinetics of MBZ and two metabolites, 2-amino-5(6)-benzoylbenzimidazole (MBZ-NH2) and 5-hydroxymebendazole (MBZ-OH), in Japanese pufferfish following intramuscular injection of 20 mg/kg MBZ. We detected three metabolites of MBZ (M1–M3), among which, 2-amino-5(6)-(a-hydroxybenzyl) benzimidazole (M3) was detected in an aquatic animal for the first time. The plasma dispositions of MBZ, MBZ-NH2, and MBZ-OH were characterized by low plasma clearance, medium distribution volume, and long terminal half-life. Moreover, these compounds were widely distributed in the muscle, from which they were rapidly cleared. The pharmacokinetics and metabolism of mebendazole in Japanese pufferfish are described for the first time in this study. Our findings provide a basis for the rational application of MBZ in Japanese pufferfish farming and contribute to our understanding of the metabolism of MBZ in cultured fish.","PeriodicalId":12121,"journal":{"name":"Food Additives & Contaminants: Part A","volume":"47 1","pages":"912 - 924"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87565819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous detection of multiple phenolic compounds in fish by gas chromatography-mass spectrometry following a modified QuEChERS cleanup","authors":"Jiali Xing, Yang Li, Ruihang Zheng, Hao Shen, Xiaorong Xu, Lingyan Mao, Xiaohu Luo, Jian Shen, Weirong Yao","doi":"10.1080/19440049.2022.2062058","DOIUrl":"https://doi.org/10.1080/19440049.2022.2062058","url":null,"abstract":"Abstract Phenolic compounds can cause health problems in humans through the food chain. Considering that fish play an important role in human diets, we established a rapid, simple and high-throughput method for the determination of 18 phenolic compounds in fish based on a modified QuEChERS sample preparation method combined with GC-MS. The average recovery of the 18 phenolic compounds was 81.3–116% at 3 spiked levels, and the relative standard deviations, RSDr and RSDwR, were in the range of 1.1–11.3% and 1.5–12.2%, respectively. The limit of detection was 2.0–10.1 μg/kg. Satisfactory linear relationships (R 2 > 0.998) were observed for the phenolic compounds in their corresponding concentration ranges. Moreover, the established method exhibited a high sensitivity, good stability, and reliability. The development of this method has an important theoretical and practical significance for establishing standards and to control the residue levels of phenolic compounds in fish.","PeriodicalId":12121,"journal":{"name":"Food Additives & Contaminants: Part A","volume":"24 1","pages":"1136 - 1148"},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73695687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold-based strip sensor for the rapid and sensitive detection of butralin in tomatoes and peppers","authors":"Xinxin Xu, Xin Guo, Shanshan Song, Aihong Wu, Chuanlai Xu, Hua Kuang, Liqiang Liu","doi":"10.1080/19440049.2022.2063391","DOIUrl":"https://doi.org/10.1080/19440049.2022.2063391","url":null,"abstract":"Abstract Butralin is a widely used dinitroaniline herbicide. Butralin residues in vegetables or fruits represent a threat to human health. In this study, we developed a rapid and sensitive gold-based lateral flow immunoassay (LFIA) for butralin detection in tomato and green pepper samples based on a screened monoclonal antibody (mAb) against butralin. The mAb possessed a half-maximal inhibitory concentration (IC50) of 12.7 ng/mL, with no cross-reactivity toward other dinitroaniline herbicides. The established LFIA strip had a visible limit of detection (LOD) of 50 ng/g and a cut-off value of 2000 ng/g in tomato and green pepper samples. According to the calibration curves for quantitative analysis, the calculated LODs of the LFIA strip were 4.7 ng/g and 4.3 ng/g in tomato and green pepper, respectively. The results were obtained within 10 min. The average recoveries ranged between 95.4% and 109.6% with a coefficient of variation (CV) of 4.3% to 7.1% in tomato samples and between 94.8% and 109.1% with a CV of 3.9% to 6.1% in green pepper samples. These data suggested that our proposed LFIA is a sensitive, specific, and reliable method for the rapid detection of butralin in real samples.","PeriodicalId":12121,"journal":{"name":"Food Additives & Contaminants: Part A","volume":"24 1 1","pages":"1255 - 1264"},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77815927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of fungicides on spike characteristics in winter wheat inoculated with Fusarium culmorum","authors":"N. D. Köycü","doi":"10.1080/19440049.2022.2052971","DOIUrl":"https://doi.org/10.1080/19440049.2022.2052971","url":null,"abstract":"Abstract The impact of fungicides on the head blight (FHB) development disease index, percent spike harvest index (SHI), and deoxynivalenol (DON) accumulation in wheat kernels under field conditions was evaluated after artificial spike inoculation with Fusarium culmorum (S-14). The trial was carried out using commercially available fungicides and a sensitive cultivar of bread wheat (Flamura-85) in a field of a wheat producer located in the Trakya region of Turkey. Fungicides were applied at the beginning of anthesis (ZGS 61), 48 hours after the inoculation with the pathogen. Disease index was determined 10 days and 14 days post-inoculation. The application of fungicides containing tebuconazole, thiophanate methyl plus tetraconazole and prothioconazole plus trifloxystrobin reduced the FHB disease and increased kernel number, spike weight, and kernel weight, as compared to the inoculated/non-fungicide control. The efficacy of tebuconazole and of prothioconazole plus trifloxystrobin was higher than that of thiophanate methyl plus tetraconazole on FHB disease severity, percent spike harvest index (SHI), and DON accumulation in wheat kernels.","PeriodicalId":12121,"journal":{"name":"Food Additives & Contaminants: Part A","volume":"10 1","pages":"1001 - 1008"},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87417251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T-2 and HT-2 toxins in harvested oat grains and their prevalence in whole grain flour during storage","authors":"S. Janavičienė, A. Mankevičienė, Y. Kochiieru, Eimantas Venslovas","doi":"10.1080/19440049.2022.2063392","DOIUrl":"https://doi.org/10.1080/19440049.2022.2063392","url":null,"abstract":"Abstract Mycotoxin contamination of food is a constant global concern. There has been a scientific debate in Europe on the validation of accredited detection methods for type A trichothecenes T-2 and HT-2 and the restriction on dangerous concentrations. The issue is of great importance as this type of mycotoxin is frequently found in spring cereals grown in Lithuania. The aim of this study was to optimise and validate a method for the determination of T-2/HT-2 toxin concentrations in oats harvested in 2015–2018 and to observe the changes in the concentrations of both toxins in oat flour during 3- and 6-week storage at different temperatures and increased relative air humidity. All of the oat grain samples (100%) collected in 2015–2018 tested positive for contamination with type A trichothecenes. The highest mean co-contamination by T-2 + HT-2 (260.4 ± 140.9 µg/kg) and the highest concentration (594.6 µg/kg) were determined in 2018 when warm and wet weather conditions prevailed during oat flowering. The effect of long-term storage (6 weeks) on T-2 and HT-2 toxin production manifested itself only when the samples had been stored under cooler conditions (8 °C). The most important factors which impacted the variation of the concentrations of type A trichothecenes in flour were ambient temperature and storage time.","PeriodicalId":12121,"journal":{"name":"Food Additives & Contaminants: Part A","volume":"27 1","pages":"1284 - 1295"},"PeriodicalIF":0.0,"publicationDate":"2022-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74522143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a method for simultaneous screening of four natural-derived steroids and their analogues used as dietary supplements via liquid chromatography-quadrupole-time of flight mass spectrometry and liquid chromatography-tandem mass spectrometry","authors":"Ji Hyun Lee, J. H. Han, Hyeon Joo Ham, Hyungil Kim, Jongkook Lee, S. Baek","doi":"10.1080/19440049.2022.2048899","DOIUrl":"https://doi.org/10.1080/19440049.2022.2048899","url":null,"abstract":"Abstract Natural-derived steroids and their analogues are present in various plants and insects. To minimize the chance of missing a positive doping test and avoiding potentially serious health problems, adequate screening methods are necessary for the detection of a wide range of natural-derived steroids and their analogues in dietary supplements. In this study, an accurate and simple liquid-chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to determine and quantify the natural-derived steroids and their analogues according to the International Conference on Harmonization of technical Requirements for Registration of Pharmaceuticals for Human Use guidelines. The validation results indicating excellent extraction efficiency and low matrix effects show that the LC-MS/MS method is reliable for the detection of natural-derived steroids and their analogues. In addition, we established the ion fragmentation of turkesterone and ion fragmentation of four natural-derived steroids and their analogues. The validated method was applied to 60 dietary supplements purchased online and in person from international vendors in 2020. Ecdysterone and 5α-hydroxylaxogenin were detected respectively in 3 and 14 of 60 dietary supplements. Especially, a high amount of 5α-hydroxylaxogenin, an FDA-unapproved ingredient, was detected in two of dietary supplements (44.4 and 32.3 mg/g). This component should be controlled since it may cause unexpected side effects if administered excessively. Thus, this method will be helpful for the continuous control and supervision of unlicensed dietary supplements containing natural-derived steroids and their analogues.","PeriodicalId":12121,"journal":{"name":"Food Additives & Contaminants: Part A","volume":"37 18 1","pages":"829 - 837"},"PeriodicalIF":0.0,"publicationDate":"2022-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78336072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal and geographical variations in insecticide and miticide residues in hop samples produced across three hop-growing regions in the Czech Republic during the years 2018–2020","authors":"M. Dušek, J. Vostřel, Kamila Kalachová, Vladimíra Jandovská","doi":"10.1080/19440049.2022.2061054","DOIUrl":"https://doi.org/10.1080/19440049.2022.2061054","url":null,"abstract":"Abstract The aim of this study was to determine the presence of zoocide (insecticide and miticide) residues in hops collected in three hop-growing regions located in the Czech Republic, and to assess their zoocide profiles and residue levels in terms of variability in temperature and precipitation across the 2018–2020 seasons. Furthermore, the weather factors that influenced the occurrence of hop pests are described and discussed. During our 3-year survey, a total of 120 samples of whole-cone hops samples harvested in three hop-growing regions were analysed for the presence of 29 insecticides and miticides using the modified QuEChERS extraction method, followed by liquid chromatography-tandem mass spectrometry and gas chromatography-tandem mass spectrometry. A majority, 119 of 120 samples, contained a residue of at least one of the active substances surveyed in this study, and 34 analysed samples contained multiple residues with three to four zoocides presented. Concerning the most frequently detected zoocide residues, spirotetramat and/or its metabolites were found in 94.2% of the samples at levels ranging from 0.02 to 1.08 mg/kg. Of the other zoocides surveyed, residues of fenpyroximate, hexythiazox, bifenazate and lambda-cyhalothrin were routinely found in hop cone samples. Obtained data were then used for evaluating seasonal and geographical variations in the profile of zoocide residues among the hop-growing regions in the years 2018–2020, and the compliance with legal regulations concerning the use of zoocides on hops was ascertained. The results showed that (1) the profile and levels of zoocide residues found in the samples reflected seasonal prevalence of pest infestation on hop plants; (2) the strategy to control pests (especially aphids) used in most of hop yards was consistent across the seasons; and (3) a concentration of spirotetramat residues less than 1 mg/kg was typical for hops grown in the Czech Republic.","PeriodicalId":12121,"journal":{"name":"Food Additives & Contaminants: Part A","volume":"56 1","pages":"1109 - 1122"},"PeriodicalIF":0.0,"publicationDate":"2022-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76922219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of serum paraoxonase/arylesterase 1 (PON1) as a protein marker of illicit dexamethasone treatment in veal calves","authors":"C. Guglielmetti, S. Brusadore, M. Pezzolato, E. Baioni, F. Ingravalle, P. Acutis, M. Mazza, E. Bozzetta","doi":"10.1080/19440049.2022.2062057","DOIUrl":"https://doi.org/10.1080/19440049.2022.2062057","url":null,"abstract":"Abstract The illicit use of dexamethasone and other glucocorticoids for cattle fattening in livestock production has been widely described; evidence for illegal treatments can be obtained by direct or indirect detection. In our previous study, we applied two-dimensional electrophoresis (2DE) to identify plasma protein markers of dexamethasone administration in veal calves. Comparison of 2DE maps obtained from blood samples before and after treatment showed the disappearance of two protein spots identified as serum paraoxonase/arylesterase 1 precursor (PON1). In the present study, we validated PON1 as a marker by analysing a larger number of samples treated with dexamethasone for illicit use. Analysis of samples from experimental treatment with other glucocorticoids, androgens and oestrogens confirmed that their influence on PON1 could be excluded. The specificity of the PON1 protein marker was verified on expected negative field samples to exclude interfering factors. However, there is poor statistical evidence to support a significant association between the outcome of PON1 and the considered variables. The results on field samples were compared with histological examination of the thymus as a biomarker of corticosteroid treatment monitored in the Italian histological plan for the control of growth promoters in animals. Two suspect cases were identified from two Piedmont farms where other animals had tested positive at histological examination. In conclusion, the absence of PON1 in the plasma of veal calves can indirectly reveal illicit dexamethasone treatment in individual animals and so identify suspect farms for further investigation. It is effective in a period ranging from 3 to about 10 days from illicit treatment, covering a time span that goes beyond the limits of official chemical controls and preceding histological controls on the thymus of slaughtered animals. PON1 detection in plasma can be coupled with other tests to identify illegal dexamethasone use on veal calf farms. Graphical Abstract","PeriodicalId":12121,"journal":{"name":"Food Additives & Contaminants: Part A","volume":"11 5 1","pages":"1065 - 1073"},"PeriodicalIF":0.0,"publicationDate":"2022-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73137904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PCDD/Fs and PCBs in Mytilus galloprovincialis from a contaminated area in Italy: the role of mussel size, temperature and meteorological factors","authors":"O. V. Giannico, S. Baldacci, F. Desiante, F. Basile, Ettore Franco, G. R. Fragnelli, G. Diletti, M. Conversano","doi":"10.1080/19440049.2022.2059108","DOIUrl":"https://doi.org/10.1080/19440049.2022.2059108","url":null,"abstract":"Abstract Mussels represent a food at risk of contamination because they are filter-feeding organisms with high bioaccumulation and low biotransformation potential for organic contaminants. Taranto, Southern Italy, is of particular relevance in this context due to the presence of industrial sources of POPs. The aim of this study was to analyse the role of mussel size, temperature and other meteorological factors in determining PCDD/Fs and PCBs concentrations in mussels. Mussels were collected on a monthly basis with random sampling between 2012 and 2020 according to the extraordinary monitoring plan of the Prevention Department. Samples were analysed by the National Reference Laboratory for Halogenated POPs in food and feed. A total of 787 mussel samples were collected between 2012 and 2020. Higher median concentrations were observed in Mar Piccolo First Inlet: PCDD/Fs 1.49 pg WHO-TEQ/g ww; PCDD/Fs + DL-PCBs 6.35 pg WHO-TEQ/g ww; DL-PCBs 4.74 pg WHO-TEQ/g ww; NDL-PCBs 65.10 ng/g ww. In all basins, PCDD/Fs + DL-PCBs, DL-PCBs and NDL-PCBs showed higher concentrations in mussels ≥ 5 cm. Temperature showed positive association with all pollutants in all basins. Higher relative humidity was associated with higher concentrations of some pollutants, while number of rain days was associated with lower values. Our findings showed higher PCDD/Fs and PCBs concentrations in adult mussels, with dependence on temperature and some other meteorological factors. These results could help guide public health strategies and mussel sampling according to mussel size and meteorological conditions in areas affected by POPs contamination.","PeriodicalId":12121,"journal":{"name":"Food Additives & Contaminants: Part A","volume":"15 1","pages":"1123 - 1135"},"PeriodicalIF":0.0,"publicationDate":"2022-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73476942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of deoxynivalenol, T-2 and HT-2 toxins and associated Fusarium species during commercial and laboratory de-hulling of milling oats","authors":"Jens Meyer, T. Birr, Inga Hennies, Detlev Wessels, K. Schwarz","doi":"10.1080/19440049.2022.2059576","DOIUrl":"https://doi.org/10.1080/19440049.2022.2059576","url":null,"abstract":"Abstract Oats (Avena sativa L.) are well known for their nutritional properties but are susceptible to the growth of different Fusarium fungi resulting in mycotoxin contamination of harvested oats. In this study, oat samples from harvest years 2011 to 2017 were preselected for their suitability as milling oats for food purposes with DON contents below 1750 µg/kg. The reduction of DON, T-2 and HT-2 toxins during the commercial de-hulling process was analysed. While the average reduction for the sum of T-2 and HT-2 toxins in large oat kernels was 85%, the reduction for thin kernels was 66%. The reduction for DON was about 60% and did not differ for the two kernel fractions. In laboratory de-hulling experiments, milling oat samples and de-hulled oat kernels with known DON, T-2 and HT-2 toxin content were correlated with the associated DNA amount of Fusarium graminearum, Fusarium culmorum and Fusarium langsethiae. The reduction of the Fusarium DNA amount after de-hulling was comparable to the reduction of the associated mycotoxins. Notably, the correlation between F. langsethiae DNA amounts and the sum of T-2 and HT-2 toxin contents was R 2 = 0.69 in milling oats and it rose to R 2 = 0.85 in de-hulled oat kernels. In laboratory tests, at least one third of the initial levels of DON and the sum of T-2 and HT-2 toxins could be removed by polishing off the first parts of the outer layers; two thirds remained in the polished oat kernels. These observations indicate that de-hulling alone may not be completely sufficient to remove mycotoxin contamination in oats. These findings are of high importance in the discussion of determining legal maximum levels for DON or the sum of T-2 and HT-2 toxins in intermediate and final products.","PeriodicalId":12121,"journal":{"name":"Food Additives & Contaminants: Part A","volume":"67 4","pages":"1163 - 1183"},"PeriodicalIF":0.0,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72621494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}