Experimental Mycology最新文献_第9页

Isolation of the ERG2 Gene, Encoding Δ8 → Δ7 Sterol Isomerase, from the Maize Smut Pathogen Ustilago maydis 玉米黑穗病病菌Δ8→Δ7甾醇异构酶编码基因ERG2的分离

Experimental Mycology Pub Date : 1994-03-01 DOI: 10.1006/emyc.1994.1009

Andrew M. Bailey, Raymond S. Burden, Caroline S. James, John P.R. Keon, Rebecca Croxen, Martin Bard, John A. Hargreaves

引用次数: 7

Gravitropism of the Basidiomycete Flammulina velutipes: Morphological and Physiological Aspects of the Graviresponse 金针菇担子菌的向重力性:重力反应的形态和生理方面

Experimental Mycology Pub Date : 1994-03-01 DOI: 10.1006/emyc.1994.1002

Jan Monzer, Eva Haindl, Volker Kern, Kerstin Dressel

{"title":"Gravitropism of the Basidiomycete Flammulina velutipes: Morphological and Physiological Aspects of the Graviresponse","authors":"Jan Monzer, Eva Haindl, Volker Kern, Kerstin Dressel","doi":"10.1006/emyc.1994.1002","DOIUrl":"10.1006/emyc.1994.1002","url":null,"abstract":"<div>Monzer J., Haindl, E., Kern V., and Dressel, K. 1994. Gravitropism of the basidiomycete Flammulina velutipes: Morphological and physiological aspects of the graviresponse. Experimental Mycology, 18, 7-19. The fruiting body of the basidiomycete Flammulina velutipes shows a distinct negative gravitropic response. Maturing fruiting bodies in the rapid elongation phase become graviresponsive with basidiospore differentiation. Lateral gravistimulation by horizontal arrangement of the fruiting body results in unilateral growth regulation. Elongation in the upper stipe side decreases to 40% during gravitropic reorientation of the fruiting body. Overshooting of the gravitropic response during reorientation is precisely regulated. The graviresponsiveness is concentrated to the apical area of the stipe, the transition zone between pileus and stipe, which features a prominent elongation capability. The small size and low vacuolization of the transition zone hyphae compared with differentiated basal stipe hyphae correspond with this physiological function on the light and electron microscopical levels. Curvature experiments using intact and explanted fruiting bodies demonstrated the graviperceptive role of the transition zone. The excision of various amounts of pilear tissue, even the disruption of the whole pileus, had no severe effect on gravitropic curvature, until the transition zone was damaged. Removal of the transition zone resulted in a dramatic loss of graviresponse, whereas the decrease of elongation was less drastic.</div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"18 1","pages":"Pages 7-19"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1994.1002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89481465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

A Statistical Analysis of Sequence Features within Genes from Neurospora crassa 粗神经孢子虫基因序列特征的统计分析

Experimental Mycology Pub Date : 1994-03-01 DOI: 10.1006/emyc.1994.1007

Stephanie E. Edelmann, Chuck Staben

引用次数: 111

Molecular Genotyping of Colletotrichum Species Based on Arbitrarily Primed PCR, A + T-Rich DNA, and Nuclear DNA Analyses 基于任意引物PCR、富A + t DNA和核DNA分析的炭疽菌分子基因分型

Experimental Mycology Pub Date : 1993-12-01 DOI: 10.1006/emyc.1993.1029

S. Freeman, M. Pham, R.J. Rodriguez

{"title":"Molecular Genotyping of Colletotrichum Species Based on Arbitrarily Primed PCR, A + T-Rich DNA, and Nuclear DNA Analyses","authors":"S. Freeman, M. Pham, R.J. Rodriguez","doi":"10.1006/emyc.1993.1029","DOIUrl":"10.1006/emyc.1993.1029","url":null,"abstract":"<div>Freeman, S., Pham, M., and Rodriguez, R. J. 1993. Molecular genotyping of Colletotrichum species based on arbitrarily primed PCR, A + T-rich DNA, and nuclear DNA analyses. Experimental Mycology 17, 309-322. Isolates of Colletotrichum were grouped into 10 separate species based on arbitrarily primed PCR (ap-PCR), A + T-rich DNA (AT-DNA) and nuclear DNA banding patterns. In general, the grouping of Colletotrichum isolates by these molecular approaches corresponded to that done by classical taxonomic identification, however, some exceptions were observed. PCR amplification of genomic DNA using four different primers allowed for reliable differentiation between isolates of the 10 species. HaeIII digestion patterns of AT-DNA also distinguished between species of Colletotrichum by generating species-specific band patterns. In addition, hybridization of the repetitive DNA element (GcpR1) to genomic DNA identified a unique set of Pst 1-digested nuclear DNA fragments in each of the 10 species of Colletotrichum tested. Multiple isolates of C. acutatum, C. coccodes, C. fragariae, C. lindemuthianum, C. magna, C. orbiculare, C. graminicola from maize, and C. graminicola from sorghum showed 86-100% intraspecies similarity based on ap-PCR and AT-DNA analyses. Interspecies similarity determined by ap-PCR and AT-DNA analyses varied between 0 and 33%. Three distinct banding patterns were detected in isolates of C. gloeosporioides from strawberry. Similarly, three different banding patterns were observed among isolates of C. musae from diseased banana.</div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"17 4","pages":"Pages 309-322"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1993.1029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89672774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 120

Substrate Hydrophobicity and Adhesion of Uromyces Urediospores and Germlings 尿真菌孢子和芽孢的底物疏水性和粘附性

Experimental Mycology Pub Date : 1993-12-01 DOI: 10.1006/EMYC.1993.1024

B. T. Terhune, H. C. Hoch

引用次数: 81

Organism Index for Volume 17 第17卷的生物索引

Experimental Mycology Pub Date : 1993-12-01 DOI: 10.1006/emyc.1993.1039

引用次数: 0

Subject Index for Volume 17 第17卷的主题索引

Experimental Mycology Pub Date : 1993-12-01 DOI: 10.1006/emyc.1993.1038

引用次数: 0

Chemical Composition of the Hyphal Wall from Fusarium graminearum 小麦镰刀菌菌丝壁的化学成分

Experimental Mycology Pub Date : 1993-12-01 DOI: 10.1006/emyc.1993.1026

Ione Parra Barbosa, Carlos Kemmelmeier

{"title":"Chemical Composition of the Hyphal Wall from Fusarium graminearum","authors":"Ione Parra Barbosa, Carlos Kemmelmeier","doi":"10.1006/emyc.1993.1026","DOIUrl":"10.1006/emyc.1993.1026","url":null,"abstract":"<div>Barbosa, I. P., and Kemmelmeier, C. 1993. Chemical composition of the hyphal wall from Fusarium graminearum. Experimental Mycology 17, 274-283. Hyphal cell walls of Fusarium graminearum (zearalenone producer strain) were isolated, purified, and subjected to chemical analysis, enzymatic degradation, and electron microscopy. Chemical analysis revealed that the cell wall contained 74.5% carbohydrate, 4.5% protein, 3.0% lipid, 4.5% ash, and 0.3% phosphorus. The major cell wall component, N-acetylglucosamine (31%), is in the form of chitin, as shown by infrared spectroscopy. Glucose constituted 21.5% of the cell wall while mannose, galactose, arabinose, and glucuronic acid accounted for 22% of the cell wall. Glucuronic acid seems to be predominantly linked to galactose in the intact wall. Electron microscopy studies showed that the isolated cell wall consisted of two distinct layers, an outer electron-dense layer and a broader electron-transparent inner layer. Furthermore, intact cells showed an additional outer mucilaginous layer. The cell wall was shown to be susceptible to enzymatic degradation with the gastric juice from the snail Megalobulimus paranaguensis.</div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"17 4","pages":"Pages 274-283"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1993.1026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78417458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Isolation of Trap Cells from the Nematode-Trapping Fungus Dactylaria candida 捕获线虫真菌假丝酵母诱捕细胞的分离

Experimental Mycology Pub Date : 1993-12-01 DOI: 10.1006/emyc.1993.1036

Eva Friman

引用次数: 14

Purification and Partial Amino Acid Sequence of the Major 70,000-Dalton Heat Shock Protein in Neurospora crassa 粗神经孢子虫70000 - dalton主要热休克蛋白的纯化及部分氨基酸序列分析

Experimental Mycology Pub Date : 1993-12-01 DOI: 10.1006/emyc.1993.1035

Franco Fracella, Saadat Mohsenzadeh, Ludger Rensing

{"title":"Purification and Partial Amino Acid Sequence of the Major 70,000-Dalton Heat Shock Protein in Neurospora crassa","authors":"Franco Fracella, Saadat Mohsenzadeh, Ludger Rensing","doi":"10.1006/emyc.1993.1035","DOIUrl":"10.1006/emyc.1993.1035","url":null,"abstract":"<div>Fracella, F., Mohsenzadeh, S., and Rensing, L. 1993. Purification and partial amino acid sequence of the major 70,000-Dalton heat shock protein in Neurospora crassa. Experimental Mycology , 17, 362-367. The major heat shock protein of 70 kDa (hsp70) from heat-shocked mycelial extracts of Neurospora crassa was purified to near homogeneity employing DEAE anion-exchange chromatography followed by affinity chromatography on ATP-agarose. The isolated hsp70 migrates as a single band on one-dimensional sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE), with a molecular mass of ∼69 kDa. On two-dimensional gels it is resolved into two polypeptides with isoelectric points in the acidic range of ∼pH 5.2. The first 53 amino terminal amino acids of the major protein were sequenced and compared with hsp70 of other species. The amino acids aspartic acid, arginine, and phenylalanine occur at positions 27, 28, and 44 (from the methionine terminus) in contrast to the main consensus sequence. These three differing amino acids are shared by yeast, and, in addition, the first two by Arabidopsis, petunia, and maize.</div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"17 4","pages":"Pages 362-367"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1993.1035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90263981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15