Isolation of the ERG2 Gene, Encoding Δ8 → Δ7 Sterol Isomerase, from the Maize Smut Pathogen Ustilago maydis

Abstract

Bailey, A. M., Burden, R. S., James, C. S., Keon, J. P. R., Croxen, R., Bard, M., and Hargreaves, J. A. 1993. Isolation of the ERG2 gene, encoding Δ⁸ → Δ⁷ sterol isomerase, from the maise smut pathogen Ustilago maydis. Experimental Mycology 18, 87-92. The ERG2 gene encoding Δ⁸ → Δ⁷ sterol isomerase has been isolated from the fungal plant pathogen Ustilago maydis. This was accomplished by screening an U. maydis genomic library with a fragment of the Saccharomyces cerevisiae ERG2 gene. The identity of the U. maydis ERG2 gene was confirmed by complementation of an U. maydis Erg2 mutant and by comparing the deduced amino acid sequence encoded by the U. maydis ERG2 gene with that of the S. cerevisiae ERG2 gene product.