Experimental Mycology最新文献_第8页

Mating-Type Incompatibility between Commercial Strains of Lentinula edodes 香菇商业品系间的交配型不亲和性

Experimental Mycology Pub Date : 1994-06-01 DOI: 10.1006/emyc.1994.1011

Hilary M Fox, John Burden, Shu-Ting Chang, John F Peberdy

引用次数: 18

Presence of GTP-Binding Proteins in the Plasma Membrane of the Phycomyces Sporangiophore 孢囊藻质膜中gtp结合蛋白的存在

Experimental Mycology Pub Date : 1994-06-01 DOI: 10.1006/emyc.1994.1015

Hassan Ashktorab, Robert J. Cohen

{"title":"Presence of GTP-Binding Proteins in the Plasma Membrane of the Phycomyces Sporangiophore","authors":"Hassan Ashktorab, Robert J. Cohen","doi":"10.1006/emyc.1994.1015","DOIUrl":"10.1006/emyc.1994.1015","url":null,"abstract":"<div>Ashktorab, H., and Cohen, R. J. 1994. Presence of GTP-binding proteins in the plasma membrane of the Phycomyces sporangiophore. Experimental Mycology 18, 139-149. When a plasma membrane-enriched fraction isolated from the sporangiophore of the Zygomycete Phycomyces blakesleeanus was subject to immunoblotting, two polypeptide bands reacted with an antibody directed to a conserved sequence of the ∝ subunit of G-proteins; their apparent molecular masses were 40 and 51 kDa. Upon treating the plasma membrane preparation with cholera toxin, bands at 40 and 51 kDa appeared to be ADP-ribosylated but no band appeared with pertussis toxin incubation. Apparent dissociation constants for the binding of GTPγS were determined for plasma membrane from Phycomyces sporangiophore grown in the light (KD = 39 ± 16 nM) (±SD) and in the dark (KD = 11 ± 6 nM). GTP served as a strong competitor for binding as did GDP, although somewhat less well, while ATP competed considerably more weakly. Northern analysis of sporangiophore mRNA displayed two bands hybridizing to the Gα2 probes coding for a Gα subunit from Dictyostelium discoideum. Furthermore, Western blotting of plasma membrane revealed several bands containing polypeptides with presumptive covalently attached immunoreactive flavins. (The prevailing evidence from the action spectra of Phycomyces is that the photoreceptor is a flavoprotein residing in the plasma membrane.) Immunoblotting also detected a H+ ATPase similar to the plasma membrane enzyme of yeast, corroborating our isolation of plasma membrane and suggesting another possible player in the signal responses of Phycomyces . This is apparently the first evidence for a G-protein in this class of eukaryotes. G-proteins may serve a role in the flavoprotein-mediated phototransduction system of P. blakesleeanus.</div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"18 2","pages":"Pages 139-149"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1994.1015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90165004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Retardation of the Growth of Transplanted Apothecia: A Manifestation of Vegetative Incompatibility in Ascobolus stercorarius (Bull.) Schröt. 移植的荷叶花生长迟缓:一种营养不相容的表现。散粒。

Experimental Mycology Pub Date : 1994-06-01 DOI: 10.1006/emyc.1994.1012

George N. Bistis

{"title":"Retardation of the Growth of Transplanted Apothecia: A Manifestation of Vegetative Incompatibility in Ascobolus stercorarius (Bull.) Schröt.","authors":"George N. Bistis","doi":"10.1006/emyc.1994.1012","DOIUrl":"10.1006/emyc.1994.1012","url":null,"abstract":"<div>Bistis, G. N. 1994. Retardation of the growth of transplanted apothecia: A manifestation of vegetative incompatibility in Ascobolus stercorarius (Bull.) Schröt. Experimental Mycology 18, 103-110. Two loci, C and D, each with at least two alleles, C1, C2 and D1, D2, control a system of apothecium-transplant incompatibility in the fungus Ascobolus stercorarius. The recognition appears to occur between the sterile tissue of the transplanted apothecium and the vegetative hyphae of the recipient mycelium. When these carry similar C and D alleles the transplanted apothecium grows at a normal rate. If they carry different D alleles the apothecium grows at a subnormal rate. If they carry different C alleles (with like or different Ds) the apothecium grows at a sub-subnormal rate. The C locus, but not the D, also has a detectable effect in vegetative heterokaryons. Two auxotrophic mutants will complement only if they carry like C factors. The two mating-type alleles, A, a, also function as transplant and vegetative incompatibility factors.</div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"18 2","pages":"Pages 103-110"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1994.1012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84445627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Genetic Analyses of Interspecific Hybrids between Phytophthora infestans and Phytophthora mirabilis 疫霉与神奇疫霉种间杂种的遗传分析

Experimental Mycology Pub Date : 1994-03-01 DOI: 10.1006/emyc.1994.1003

Stephen B Goodwin, William E Fry

{"title":"Genetic Analyses of Interspecific Hybrids between Phytophthora infestans and Phytophthora mirabilis","authors":"Stephen B Goodwin, William E Fry","doi":"10.1006/emyc.1994.1003","DOIUrl":"10.1006/emyc.1994.1003","url":null,"abstract":"<div>Goodwin, S. B., and Fry, W. E. 1994. Genetic analyses of interspecific hybrids between Phytophthora infestans and Phytophthora mirabilis. Experimental Mycology 18, 20-32. Four crosses were made between isolates of two host-specific Phytophthora species. Phytophthora infestans and Phytophthora mirabilis. In the two most successful crosses involving a common P. infestans A2 parent, allozyme analysis confirmed that 79 of 86 progeny were interspecific hybrids, 3 were presumed selfs, and 4 were either selfs or nonrecombinant parental types. Mating type, alleles at the allozyme locus glucose-6-phosphate isomerase, and the + alleles at a number of DNA fingerprinting loci segregated independently according to Mendelian expectation. Three DNA fingerprinting loci were tightly linked in P. mirabilis, but no other linkages were detected among these markers. Mitochondrial DNA was uniparentally inherited, mostly from the P. infestans parent. Growth rate segregated as a quantitative character. None of the 68 progeny tested infected Mirabilis jalapa (the host of P. mirabilis), 3 infected potato, and 4 were weakly pathogenic to tomato. Because most of the F1 hybrids could not infect any of the hosts infected by the parents, host specialization could provide a postzygotic as well as a prezygotic reproductive isolating mechanism for P. infestans and P. mirabilis in central Mexico. These results indicate that P. mirabilis probably is capable of a regular outcrossing mating system.</div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"18 1","pages":"Pages 20-32"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1994.1003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86219734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 94

Plasmid Loss in Agaricus bitorquis without Alterations in Homologous Mitochondrial Sequences 同源线粒体序列未改变的松茸质粒丢失

Experimental Mycology Pub Date : 1994-03-01 DOI: 10.1006/emyc.1994.1008

Mary M. Robison, Paul A. Horgen

引用次数: 5

Genetic Variation in Heterobasidion annosum Detected with M13 Fingerprinting and Ribosomal DNA Probes M13指纹图谱和核糖体DNA探针检测杂交虫的遗传变异

Experimental Mycology Pub Date : 1994-03-01 DOI: 10.1006/emyc.1994.1005

Jan-Olof Karlsson

引用次数: 25

The Behavior of F-Actin during the Zoosporic Phases of the Chytridiomycete Gut Fungi Neocallimastix and Orpinomyces 壶菌属肠道真菌Neocallimastix和Orpinomyces动物孢子期F-Actin的行为

Experimental Mycology Pub Date : 1994-03-01 DOI: 10.1006/emyc.1994.1006

Jinliang Li, I.Brent Heath

{"title":"The Behavior of F-Actin during the Zoosporic Phases of the Chytridiomycete Gut Fungi Neocallimastix and Orpinomyces","authors":"Jinliang Li, I.Brent Heath","doi":"10.1006/emyc.1994.1006","DOIUrl":"10.1006/emyc.1994.1006","url":null,"abstract":"<div>Li, J., and Heath, I. B. 1994. The behavior of F-actin during the zoosporic phases of the chytridiomycete gut fungi Neocallimastix and Orpinomyces; Experimental Mycology 18, 57-69. The changing patterns of F-actin were shown with rhodamine-labeled phalloidin staining of cells fixed at different stages of their development cycle. Zoospores were permeated by diffuse F-actin which was more concentrated at the plasmalemma and in amoeboid projections, suggestive of a role in zoospore morphogenesis. Upon encystment, nuclei became enclosed in a perinuclear shell of F-actin which persisted throughout vegetative growth, including mitosis. We suggest that this shell, and cytoplasmic F-actin filaments, function in nuclear motility and positioning, and the former may also generate nuclear shape at mitosis. Also present during growth were peripheral F-actin plaques specifically associated with both diffuse cell wall deposition over the entire surface of the expanding cell body and localized deposition at rhizoid tips. Similar plaques are previously reported associated with localized wall synthesis in other fungi; their occurrence is now extended to the chytridiomycetes with their diffuse pattern of wall synthesis. During zoosporogenesis, cytoplasmic cleavage vacuoles were associated with extensive F-actin sheets which presumably generate or direct their expansion. Presumptive initials of these vacuoles are also F-actin-rich, consistent with an F-actin role in their initial clustering around the centrioles. Comparison of these observations with previous ones on similar stages in the unrelated oomycetes shows similarities in F-actin associated with wall synthesis, zoospore morphogenesis, and cytokinesis but differences in nuclear-associated F-actin, suggesting that an F-actin role in the former processes is a widespread and ancient phenomenon.</div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"18 1","pages":"Pages 57-69"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1994.1006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79401212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Acknowledgment 鸣谢

Experimental Mycology Pub Date : 1994-03-01 DOI: 10.1006/emyc.1994.1010

引用次数: 0

Uptake of Glucose and Phosphorus by Growing Colonies of Fusarium oxysporum as Quantified by Image Analysis 利用图像分析定量测定尖孢镰刀菌生长菌落对葡萄糖和磷的吸收

Experimental Mycology Pub Date : 1994-03-01 DOI: 10.1006/emyc.1994.1004

Stefan Olsson

{"title":"Uptake of Glucose and Phosphorus by Growing Colonies of Fusarium oxysporum as Quantified by Image Analysis","authors":"Stefan Olsson","doi":"10.1006/emyc.1994.1004","DOIUrl":"10.1006/emyc.1994.1004","url":null,"abstract":"<div>Olsson, S. 1994. Uptake of glucose and phosphorus by growing colonies of Fusarium oxysporum as quantified by image analysis. Experimental Mycology 18, 33-47. The simplest of all heterogeneous environments for fungal colony growth is the petri dish with an agar medium. As the colony grows there will be a depression of nutrient concentrations under the colony caused by the uptake of nutrients by the growing colony. Image analysis methods have been developed for measuring medium concentrations of glucose and phosphorus with simultaneous biomass density determinations in agar systems. Maps of the concentrations in the agar medium under the colony and of colony biomass density were produced. A new method for weighing fungal colonies grown on agar is also presented. For Fusarium oxysporum phosphorus and glucose uptake from the medium was the same irrespective of the C/mineral ratios in the medium within the measured range of ratios. Even the concentration profiles of the nutrients under the colony were the same irrespective of nutrient ratios. Distribution of biomass density was affected by differences in glucose concentrations, being highest at the colony margin at the lower concentrations. The results indicate that the fungal colony is able to take up nutrients at the margin in excess of the local needs.</div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"18 1","pages":"Pages 33-47"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1994.1004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79383546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Effect of n-Alkanols on Acidification Curves of Aureobasidium pullulans Suspensions 正烷醇对普鲁兰毛霉悬浮液酸化曲线的影响

Experimental Mycology Pub Date : 1994-03-01 DOI: 10.1006/emyc.1994.1001

Marı́a Dolores Moragues, Juan José Estevez, Aitor Rementerı́a, Marı́a Jesús Sevilla

{"title":"Effect of n-Alkanols on Acidification Curves of Aureobasidium pullulans Suspensions","authors":"Marı́a Dolores Moragues, Juan José Estevez, Aitor Rementerı́a, Marı́a Jesús Sevilla","doi":"10.1006/emyc.1994.1001","DOIUrl":"10.1006/emyc.1994.1001","url":null,"abstract":"<div>Moragues, M. D., Estevez, J. J., Rementerı́a, A., and Sevilla, M. J. 1994. Effect of n-alkanols on acidification curves of Aureobasidium pullulans suspensions. Experimental Mycology 18, 1-6. n -Alkanols, from methanol to 1-hexanol, induce yeast-to-hyphae transition in the dimorphic fungus Aureobasidium pullulans. In order to elucidate whether triggering of the morphogenetic event is membrane related, we have studied the effect of the morphogenetic n-alkanols on the pH of suspensions of A. pullulans, with no external carbon source. n -Alkanols, at their hyphal inducing concentration or higher, caused a decrease in the initial acidification rate (C) of yeast-phase cell suspensions of A. pullulans. From this effect on C, an inhibition coefficient (K) was deduced, specific for each alcohol. These coefficients were directly related to the lipid/buffer partition coefficients of the alkanols. On the other hand, germ tubes of A. pullulans, obtained in the presence of n-propanol, showed a much slower acidification rate in water than yeast cells. Moreover, 1-propanol or 1-butanol did not significantly affect the initial acidification rate of germ tubes. The latter observation was interpreted as an adaptation of cells grown in the presence of alcohol. The results of all these experiments support our hypothesis that the plasma membrane is a target of the morphogenetic effects of n-alkanols.</div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"18 1","pages":"Pages 1-6"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1994.1001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79342415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9