Andrew M. Bailey, Raymond S. Burden, Caroline S. James, John P.R. Keon, Rebecca Croxen, Martin Bard, John A. Hargreaves
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引用次数: 7
摘要
贝利，A. M.，伯顿，R. S.，詹姆斯，C. S.，基恩，J. P. R.，克罗森，R.，巴德，M.和哈格里夫斯，J. A. 1993。玉米黑穗病菌Δ8→Δ7甾醇异构酶编码基因ERG2的分离。实验真菌学18,87-92。编码Δ8→Δ7甾醇异构酶的ERG2基因从植物真菌病原菌黑穗病菌中分离得到。这是通过筛选含有酿酒酵母ERG2基因片段的酵母基因组文库来完成的。通过对一个霉霉ERG2突变体进行互补，并将推导出的霉霉ERG2基因编码的氨基酸序列与酿酒葡萄球菌ERG2基因产物的氨基酸序列进行比较，证实了霉霉ERG2基因的同源性。
Isolation of the ERG2 Gene, Encoding Δ8 → Δ7 Sterol Isomerase, from the Maize Smut Pathogen Ustilago maydis
Bailey, A. M., Burden, R. S., James, C. S., Keon, J. P. R., Croxen, R., Bard, M., and Hargreaves, J. A. 1993. Isolation of the ERG2 gene, encoding Δ8 → Δ7 sterol isomerase, from the maise smut pathogen Ustilago maydis. Experimental Mycology 18, 87-92. The ERG2 gene encoding Δ8 → Δ7 sterol isomerase has been isolated from the fungal plant pathogen Ustilago maydis. This was accomplished by screening an U. maydis genomic library with a fragment of the Saccharomyces cerevisiae ERG2 gene. The identity of the U. maydis ERG2 gene was confirmed by complementation of an U. maydis Erg2 mutant and by comparing the deduced amino acid sequence encoded by the U. maydis ERG2 gene with that of the S. cerevisiae ERG2 gene product.
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