Experimental and clinical endocrinology最新文献_第8页

Experimental "chronic" African trypanosomiasis: endocrine dysfunctions generated by parasitic components released during the tryptanolytic phase in rats. 实验性“慢性”非洲锥虫病:由大鼠在胰蛋白酶溶解期释放的寄生成分产生的内分泌功能障碍。

Experimental and clinical endocrinology Pub Date : 1993-01-01 DOI: 10.1055/s-0029-1211225

B Soudan, D Tetaert, M Hublart, A Racadot, D Croix, A Boersma

{"title":"Experimental \"chronic\" African trypanosomiasis: endocrine dysfunctions generated by parasitic components released during the tryptanolytic phase in rats.","authors":"B Soudan, D Tetaert, M Hublart, A Racadot, D Croix, A Boersma","doi":"10.1055/s-0029-1211225","DOIUrl":"https://doi.org/10.1055/s-0029-1211225","url":null,"abstract":"The disorders of the gonadotropic axis have been studied during the course of a \"chronic\" african trypanosomiasis induced experimentally in rats inoculated by the variant Trypanosoma brucei brucei AnTat 1.1.E. The levels of serum and pituitary LH as well as serum testosterone and corticosterone have been determined, during the infestation, at a particular period of the circadian cycle, in regard to the parasitemia variations. In addition, the inoculation of trypanosomal component fractions [obtained by concanavalin-A sepharose chromatography (conA-components)], has been performed in an attempt to define more exactly the nature of factor(s) producing the hypotestosteronemia in rats. This work evidenced that the hormonal parameter levels were predominantly decreased at the trypanolytic phase during the evolution of the disease. The action towards the hypothalamo-pituitary gonadal axis was attributed not only to peculiar trypanosomal enzyme(s) [a serine, thiol-dependent, cation sensitive endoprotease with a post-proline cleaving activity (purified from unretained conA fraction)], but also to protein and/or glycoprotein factor(s) released by the trypanosomes (components with affinity to the lectin).","PeriodicalId":12104,"journal":{"name":"Experimental and clinical endocrinology","volume":"101 3","pages":"166-72"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0029-1211225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19213528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Type II iodothyronine 5'-deiodinase in mouse brown adipose tissue is stimulated by a single injection of isoproterenol in an oil/water emulsion. 在油/水乳液中单次注射异丙肾上腺素刺激小鼠棕色脂肪组织中的II型碘甲状腺原氨酸5'-去碘酶。

Experimental and clinical endocrinology Pub Date : 1993-01-01 DOI: 10.1055/s-0029-1211232

A Lerchl, K A Stokkan, K O Nonaka, M K Vaughan, R J Reiter

引用次数: 0

Endocrine markers in malignant tumor cells producing parathyroid hormone-related protein. 恶性肿瘤细胞中分泌甲状旁腺激素相关蛋白的内分泌标志物。

Experimental and clinical endocrinology Pub Date : 1993-01-01 DOI: 10.1055/s-0029-1211247

M Zabel, M Dietel

引用次数: 5

In vivo assessment of insulin binding in different organs of growing and adult glutamate-induced obese rats. 谷氨酸诱导肥胖大鼠不同器官胰岛素结合的体内评价。

Experimental and clinical endocrinology Pub Date : 1993-01-01 DOI: 10.1055/s-0029-1211235

P Nenoff, H Remke, F Müller, T Arndt, T Mothes

{"title":"In vivo assessment of insulin binding in different organs of growing and adult glutamate-induced obese rats.","authors":"P Nenoff, H Remke, F Müller, T Arndt, T Mothes","doi":"10.1055/s-0029-1211235","DOIUrl":"https://doi.org/10.1055/s-0029-1211235","url":null,"abstract":"Injection of Na-L-glutamate into neonate Wistar-rats (2 mg/g body mass s.c.; day 1-5 of life) induces hypothalamic lesions, which are followed by hypoplastic-hypertrophic obesity despite normophagia. In contrast to other animal models of obesity, these rats develop obesity under peripheral normoinsulinemic conditions. However, beginning at an age of 2 months (growing rats), peripheral insulin concentration rises gradually and at an age of 6 months (adults rats) hyperinsulinemia becomes manifest. Surprisingly, adult rats show normoglycemia, pointing to alterations in insulin sensitivity. In continuation to previous work, insulin binding of different organs of growing and adult rats was investigated using the in vivo radioreceptor assay described by Whitcomb et al. in 1985. In contrast to in vitro methods, this assay works under real metabolic and hormonal conditions in plasma of lean and obese rats. Insulin binding of liver, pancreas, adrenals, stomach, duodenum, spleen, and heart muscles was found to be not statistically different between lean and obese rats of both age groups. Thus, liver insulin binding was 6323 +/- 458 pg/g wet organ in growing, and 7586 +/- 959 pg/g in adult lean rats. Corresponding values for obese rats were 5755 +/- 445 pg/g and 7830 +/- 526 pg/g, respectively. Organ specific down regulation of insulin binding in obese rats was not detected, suggesting unalterated insulin sensitivity. It is concluded that hyperinsulinemia of adult glutamate-induced obese rats cannot be explained by diminished insulin binding and reduced organ specific insulin clearance.","PeriodicalId":12104,"journal":{"name":"Experimental and clinical endocrinology","volume":"101 4","pages":"215-21"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0029-1211235","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18904121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Quality of life in patients with Addison's disease: effects of different cortisol replacement modes. Addison病患者的生活质量:不同皮质醇替代方式的影响

Experimental and clinical endocrinology Pub Date : 1993-01-01 DOI: 10.1055/s-0029-1211215

M Riedel, A Wiese, T H Schürmeyer, G Brabant

{"title":"Quality of life in patients with Addison's disease: effects of different cortisol replacement modes.","authors":"M Riedel, A Wiese, T H Schürmeyer, G Brabant","doi":"10.1055/s-0029-1211215","DOIUrl":"https://doi.org/10.1055/s-0029-1211215","url":null,"abstract":"This study compares the impact of different modes of cortisol replacement therapy on the health perception and general well-being in patients with primary adrenocortical failure. 14 adults (8 female, 6 male) with Addison's disease on chronic cortisol replacement participated in the study. In a randomized double-blind cross-over design, all patients were treated with 3 modes of cortisol replacement for one week each (mode I: 20 mg hydrocortisone (HC) at 0700 h and 10 mg HC at 1900 h; mode II: 30 mg HC at 0700 h and placebo at 1900 h; mode III: placebo at 0700 h and 30 mg HC at 1900 h). Following the third week, the replacement modes were repeated in a different random order. For quality-of-life assessment the patients completed three different questionnaires (Addison-questionnaire, Basler Befindlichkeits-Skala, Beschwerde-Liste) and were interviewed about their general contentment at the last day of each treatment week. General well-being in terms of subjective contentment was best established during mode I (in 64% of patients) and less often stated with mode II (in 29%) and III (in 14%) (p < 0.05 mode I vs III). With the twice-daily replacement (mode I), sum scores of all questionnaires were changed towards improvement compared to both once-daily regimens (p < 0.05 vs mode II and III), but did not reach normal values of healthy subjects. Differences between mode II and III were insignificant. We conclude that quality of life in Addison patients is mainly influenced by the mode of cortisol replacement therapy.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":12104,"journal":{"name":"Experimental and clinical endocrinology","volume":"101 2","pages":"106-11"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0029-1211215","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19390454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 58

Genetic screening for multiple endocrine neoplasia type 2. 2型多发性内分泌瘤的遗传筛查。

Experimental and clinical endocrinology Pub Date : 1993-01-01 DOI: 10.1055/s-0029-1211208

B A Ponder

引用次数: 2

Perspectives in gene therapy with recombinant adenoviruses. 重组腺病毒基因治疗的展望。

Experimental and clinical endocrinology Pub Date : 1993-01-01 DOI: 10.1055/s-0029-1211201

W Siegfried

引用次数: 23

Recent advances in the molecular biology of growth-hormone secreting human pituitary tumours. 分泌生长激素的人垂体肿瘤分子生物学研究进展。

Experimental and clinical endocrinology Pub Date : 1993-01-01 DOI: 10.1055/s-0029-1211202

E F Adams, M Buchfelder, A Hüttner, S Moreth, R Fahlbusch

引用次数: 3

Entrainment of nocturnal pituitary-adrenocortical activity to sleep processes in man--a hypothesis. 人类夜间垂体-肾上腺皮质活动对睡眠过程的影响——一种假说。

Experimental and clinical endocrinology Pub Date : 1993-01-01 DOI: 10.1055/s-0029-1211243

H L Fehm, E Späth-Schwalbe, R Pietrowsky, W Kern, J Born

{"title":"Entrainment of nocturnal pituitary-adrenocortical activity to sleep processes in man--a hypothesis.","authors":"H L Fehm, E Späth-Schwalbe, R Pietrowsky, W Kern, J Born","doi":"10.1055/s-0029-1211243","DOIUrl":"https://doi.org/10.1055/s-0029-1211243","url":null,"abstract":"The 24 hr patterns of plasma ACTH and cortisol concentrations are characterized by prominent circadian and ultradian oscillations. Usually both, nadir and acrophase of the circadian rhythm occur during sleep. This led us to re-evaluated the temporal relationship between sleep processes and nocturnal plasma ACTH and cortisol levels and the impact of several types of sleep manipulation (sleep delay, sleep disruption, sleep prolongation, sleep deprivation, and reversal of the sleep-wake cycle). Pituitary-adrenocortical activity appeared to be linked to the cyclic process of nocturnal sleep with inhibitory influences present during the first two sleep cycles. After initiation of the third sleep cycle stimulatory effects of sleep prevailed, lasting until awakening. The sleep associated influences appeared to act in concert with influences of circadian oscillators and resulted in an amplification of the circadian rhythm of pituitary-adrenal activity; they were strong enough to entrain the circadian rhythm of the pituitary-adrenal system to the sleep-wake cycle, as long as phase delays were moderate. However, with acute sleep-wake reversals the sleep associated influences were masked by the dominant effects of the circadian clock. In contrast, GH secretion appeared to be controlled primarily be sleep-associated mechanism with only minor circadian influences.","PeriodicalId":12104,"journal":{"name":"Experimental and clinical endocrinology","volume":"101 5","pages":"267-76"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0029-1211243","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19286287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Effect of protein deficiency on luteinizing hormone releasing hormone (LHRH), gonadotropin releasing hormone associated peptide (GAP) and luteinizing hormone (LH) immunocytochemistry in the hypothalamus and pituitary gland of prepubertal ewes. 蛋白质缺乏对青春期前母羊下丘脑和垂体促黄体生成素释放激素(LHRH)、促性腺激素释放激素相关肽(GAP)和促黄体生成素(LH)免疫细胞化学的影响

Experimental and clinical endocrinology Pub Date : 1993-01-01 DOI: 10.1055/s-0029-1211237

J Polkowska, F Przekop

{"title":"Effect of protein deficiency on luteinizing hormone releasing hormone (LHRH), gonadotropin releasing hormone associated peptide (GAP) and luteinizing hormone (LH) immunocytochemistry in the hypothalamus and pituitary gland of prepubertal ewes.","authors":"J Polkowska, F Przekop","doi":"10.1055/s-0029-1211237","DOIUrl":"https://doi.org/10.1055/s-0029-1211237","url":null,"abstract":"Growing female lambs were fed diets containing 14.2% (standard) or 8.1% (protein restricted) of proteins to determine their effects on puberty and luteinizing hormone releasing hormone (LHRH), gonadotropin hormone associated peptide (GAP), luteinizing hormone (LH) hormonal system. At the end of the experiment (30-34 weeks of age), hypothalamic LHRH, GAP and pituitary LH were analysed by immunocytochemical methods using specific antibodies. Plasma LH were determined by radioimmunoassay at 21 weeks of age. It was found that lowering of the dietary proteins content decreased the concentration of basal plasma LH significantly in lambs of 21 weeks of age. None of the sheep of this group reached sexual maturity at the same time as the animals of the standard group. However, immunoreactive (ir) LHRH neuronal system of protein restricted lambs was normally developed: Numerous irLHRH perikarya, dense network of axons and abundant material stored in the nerve terminals were well visualized in the typical sites of the preoptico-septal area, hypothalamus and the median eminence (ME). Gonadotropin associated peptide (GAP) of the LHRH precursor was present in the same populations of neurons that contained LHRH in the sheep brain. The proportion of pituitary LH-cells was three fold higher in pituitaries of the nutritionally restricted group. They displayed hypertrophy and very strong immunoreaction. These results show that protein deficiency in diets of growing female sheep delays their puberty but does not impair the synthesis and processing of LHRH in the brain neurons and synthesis of LH in pituitary cells.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":12104,"journal":{"name":"Experimental and clinical endocrinology","volume":"101 4","pages":"230-7"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1055/s-0029-1211237","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19295487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16