Experimental "chronic" African trypanosomiasis: endocrine dysfunctions generated by parasitic components released during the tryptanolytic phase in rats.
B Soudan, D Tetaert, M Hublart, A Racadot, D Croix, A Boersma
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引用次数: 8
Abstract
The disorders of the gonadotropic axis have been studied during the course of a "chronic" african trypanosomiasis induced experimentally in rats inoculated by the variant Trypanosoma brucei brucei AnTat 1.1.E. The levels of serum and pituitary LH as well as serum testosterone and corticosterone have been determined, during the infestation, at a particular period of the circadian cycle, in regard to the parasitemia variations. In addition, the inoculation of trypanosomal component fractions [obtained by concanavalin-A sepharose chromatography (conA-components)], has been performed in an attempt to define more exactly the nature of factor(s) producing the hypotestosteronemia in rats. This work evidenced that the hormonal parameter levels were predominantly decreased at the trypanolytic phase during the evolution of the disease. The action towards the hypothalamo-pituitary gonadal axis was attributed not only to peculiar trypanosomal enzyme(s) [a serine, thiol-dependent, cation sensitive endoprotease with a post-proline cleaving activity (purified from unretained conA fraction)], but also to protein and/or glycoprotein factor(s) released by the trypanosomes (components with affinity to the lectin).
在大鼠接种变异布鲁氏锥虫AnTat 1.1.E后实验性诱发的“慢性”非洲锥虫病过程中,研究了促性腺轴的紊乱。血清和垂体LH水平以及血清睾酮和皮质酮水平已经确定,在侵染期间,在生理周期的特定时期,关于寄生虫病的变化。此外,接种锥虫体组分组分[通过豆豆蛋白- a - sepharose色谱法(cona组分)获得],已经进行了尝试,以更准确地定义产生大鼠低睾酮血症的因素的性质。这项工作证明了在疾病的进化过程中,激素参数水平主要在锥虫分解阶段下降。对下丘脑-垂体性腺轴的作用不仅归因于特有的锥虫酶(一种丝氨酸、硫醇依赖、阳离子敏感的内蛋白酶,具有脯氨酸后切割活性(从未保留的conA部分纯化)),还归因于锥虫释放的蛋白质和/或糖蛋白因子(与凝集素有亲和力的成分)。