FASEB bioAdvances最新文献

{"title":"Unraveling the ultrastructure and dynamics of autophagic vesicles: Insights from advanced imaging techniques","authors":"Ting Jiang,&nbsp;Chaoye Ma,&nbsp;Hao Chen","doi":"10.1096/fba.2024-00035","DOIUrl":"10.1096/fba.2024-00035","url":null,"abstract":"<p>Autophagy, an intracellular self-degradation process, is governed by a complex interplay of signaling pathways and interactions between proteins and organelles. Its fundamental purpose is to efficiently clear and recycle cellular components that are damaged or redundant. Central to this process are autophagic vesicles, specialized structures that encapsulate targeted cellular elements, playing a pivotal role in autophagy. Despite growing interest in the molecular components of autophagic machinery and their regulatory mechanisms, capturing the detailed ultrastructural dynamics of autophagosome formation continues to present significant challenges. However, recent advancements in microscopy, particularly in electron microscopy, have begun to illuminate the dynamic regulatory processes underpinning autophagy. This review endeavors to provide an exhaustive overview of contemporary research on the ultrastructure of autophagic processes. By synthesizing observations from diverse technological methodologies, this review seeks to deepen our understanding of the genesis of autophagic vesicles, their membrane origins, and the dynamic alterations that transpire during the autophagy process. The aim is to bridge gaps in current knowledge and foster a more comprehensive comprehension of this crucial cellular mechanism.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":"6 7","pages":"189-199"},"PeriodicalIF":2.5,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2024-00035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141022081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiac lipotoxicity and fibrosis underlie impaired contractility in a mouse model of metabolic dysfunction-associated steatotic liver disease","authors":"Olufunto O. Badmus,&nbsp;Alexandre A. da Silva,&nbsp;Xuan Li,&nbsp;Lucy C. Taylor,&nbsp;Jennifer R. Greer,&nbsp;Andrew R. Wasson,&nbsp;Karis E. McGowan,&nbsp;Parth R. Patel,&nbsp;David E. Stec","doi":"10.1096/fba.2023-00139","DOIUrl":"10.1096/fba.2023-00139","url":null,"abstract":"<p>The leading cause of death among patients with metabolic dysfunction-associated steatotic liver disease (MASLD) is cardiovascular disease. A significant percentage of MASLD patients develop heart failure driven by functional and structural alterations in the heart. Previously, we observed cardiac dysfunction in hepatocyte-specific peroxisome proliferator-activated receptor alpha knockout (<i>Ppara</i>^HepKO), a mouse model that exhibits hepatic steatosis independent of obesity and insulin resistance. The goal of the present study was to determine mechanisms that underlie hepatic steatosis-induced cardiac dysfunction in <i>Ppara</i>^HepKO mice. Experiments were performed in 30-week-old <i>Ppara</i>^HepKO and littermate control mice fed regular chow. We observed decreased cardiomyocyte contractility (0.17 ± 0.02 vs. 0.24 ± 0.02 μm, <i>p</i> &lt; 0.05), increased cardiac triglyceride content (0.96 ± 0.13 vs. 0.68 ± 0.06 mM, <i>p</i> &lt; 0.05), collagen type 1 (4.65 ± 0.25 vs. 0.31 ± 0.01 AU, <i>p</i> &lt; 0.001), and collagen type 3 deposition (1.32 ± 0.46 vs. 0.05 ± 0.03 AU, <i>p</i> &lt; 0.05). These changes were associated with increased apoptosis as indicated by terminal deoxynucleotidyl transferase dUTP nick end labeling staining (30.9 ± 4.7 vs. 13.1 ± 0.8%, <i>p</i> &lt; 0.006) and western blots showing increased cleaved caspase-3 (0.27 ± 0.006 vs. 0.08 ± 0.01 AU, <i>p</i> &lt; 0.003) and pro-caspase-3 (5.4 ± 1.5 vs. 0.5 ± 0.3 AU, <i>p</i> &lt; 0.02), B-cell lymphoma protein 2-associated X (0.68 ± 0.07 vs. 0.04 ± 0.04 AU, <i>p</i> &lt; 0.001), and reduced B-cell lymphoma protein 2 (0.29 ± 0.01 vs. 1.47 ± 0.54 AU, <i>p</i> &lt; 0.05). We further observed elevated circulating natriuretic peptides and exercise intolerance in <i>Ppara</i>^HepKO mice when compared to controls. Our data demonstrated that lipotoxicity, and fibrosis underlie cardiac dysfunction in MASLD.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":"6 5","pages":"131-142"},"PeriodicalIF":2.7,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2023-00139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140372197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FASEB BioAdvances announces changes in 2024","authors":"Loren E. Wold,&nbsp;Crislyn D'Souza-Schorey,&nbsp;Yung Hou Wong","doi":"10.1096/fba.2024-00043","DOIUrl":"10.1096/fba.2024-00043","url":null,"abstract":"&lt;p&gt;In 2019, The Federation of American Societies for Experimental Biology (FASEB) started publishing &lt;i&gt;FASEB BioAdvances&lt;/i&gt; as a fully open-access partner to its flagship &lt;i&gt;FASEB Journal&lt;/i&gt; for authors to publish their quality research spanning the breadth of the biological and biomedical sciences. Today, the journal publishes a variety of manuscript types, including original research, reviews, and perspectives on current issues in science and academia. The scope of &lt;i&gt;FASEB BioAdvances&lt;/i&gt; overlaps with &lt;i&gt;The FASEB Journal&lt;/i&gt;, and although its priority is also quality science, it puts less emphasis on perceived impact (e.g., is a sound science journal) and now also welcomes manuscript types that we believe will improve rigor and reproducibility, including replication studies or reports on negative (null) findings.&lt;/p&gt;&lt;p&gt;Drs. Crislyn D'Souza-Schorey and Yung Hou Wong have served as Editors-in-Chief since January 2022. During their tenure, they have focused their efforts on timely review and publication of cutting-edge science, cross-disciplinary science, and original research. Their efforts have repositioned the journal with a focus that serves it well into the future.&lt;/p&gt;&lt;p&gt;Recently, FASEB considered how to best grow published output in &lt;i&gt;FASEB BioAdvances&lt;/i&gt; without reducing quality or compromising on scientific integrity. FASEB's analysis also identified a need for a seamless process for authors transferring manuscripts from &lt;i&gt;The FASEB Journal&lt;/i&gt; to &lt;i&gt;FASEB BioAdvances&lt;/i&gt; in a way that further strengthens their partnership. In an effort to increase the value of &lt;i&gt;FASEB BioAdvances&lt;/i&gt; to its authors, to decrease the time to first decision, and to better align with the needs of the global author community served by FASEB, we are announcing a change in the editorial structure of the journal. Since February 2024, &lt;i&gt;FASEB BioAdvances&lt;/i&gt; is under the direction of Loren E. Wold, PhD, Editor-in-Chief of &lt;i&gt;The FASEB Journal&lt;/i&gt; who will work to strengthen both journals. Dr. Wold and team are working on enhancing manuscript transfer options for authors, adding a new dedicated Referral Editor to work between both journals to be a resource for authors, and a plan to introduce greater emphasis on the publication of thematic special collections in areas of considerable importance. &lt;i&gt;The FASEB Journal&lt;/i&gt; is fortunate to already have in place a team of over 200 dedicated and diverse researchers: a Senior Editor, a team of eight Associate Editors, and a Special Issues and Reviews Editor, an almost 100-member editorial board, and a 116-member early career researcher editorial board. The &lt;i&gt;FASEB BioAdvances&lt;/i&gt; Deputy Editor will continue to serve in that role and will join &lt;i&gt;The FASEB Journal&lt;/i&gt; editorial team. Associate Editors of &lt;i&gt;FASEB BioAdvances&lt;/i&gt; will continue to serve in their roles. Leveraging this new, broad, and expanded editorial team for both journals will be an important advantage for authors by ensuring rapid review, edi","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":"6 4","pages":"103-104"},"PeriodicalIF":2.7,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2024-00043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140227217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The m6 RNA methylation regulator KIAA1429 is associated with autophagy-mediated drug resistance in lung cancer","authors":"Bo Ma,&nbsp;Lei Xiu,&nbsp;Lili Ding","doi":"10.1096/fba.2023-00083","DOIUrl":"10.1096/fba.2023-00083","url":null,"abstract":"<p>N6-methyladenosine (m6A) modification plays a crucial role in cancer progression. However, the role of m6A modification-mediated autophagy underlying non-small cell lung cancer (NSCLC) gefitinib resistance remains unknown. Here, we discovered that m6A methyltransferase KIAA1429 was highly expressed in NSCLC gefitinib-resistant cells (PC9-GR) as well as tissues, and KIAA1429 high expression was associated with poor survival. In addition, silent KIAA1429 repressed gefitinib resistance in NSCLC and reduced tumor growth in vivo. Mechanistically, KIAA1429 stabilized WTAP, a significant player in autophagy, by binding to the 3′ untranslated regions (3′-UTR) of WTAP. In a word, our findings indicated that KIAA1429 could elevate NSCLC gefitinib resistance, which may provide a promising targeted therapy for NSCLC patients.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":"6 4","pages":"105-117"},"PeriodicalIF":2.7,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2023-00083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progresses in gut microbiome mediates obstructive sleep apnea-induced cardiovascular diseases","authors":"Xiaotong Zhang,&nbsp;Haifen Zhang,&nbsp;Shuai Li,&nbsp;Fan Fang,&nbsp;Yanran Yin,&nbsp;Qiang Wang","doi":"10.1096/fba.2023-00153","DOIUrl":"10.1096/fba.2023-00153","url":null,"abstract":"<p>Obstructive sleep apnea (OSA) is a multifactorial sleep disorder with a high prevalence in the general population. OSA is associated with an increased risk of developing cardiovascular diseases (CVDs), particularly hypertension, and is linked to worse outcomes. Although the correlation between OSA and CVDs is firmly established, the mechanisms are poorly understood. Continuous positive airway pressure is primary treatment for OSA reducing cardiovascular risk effectively, while is limited by inadequate compliance. Moreover, alternative treatments for cardiovascular complications in OSA are currently not available. Recently, there has been considerable attention on the significant correlation between gut microbiome and pathophysiological changes in OSA. Furthermore, gut microbiome has a significant impact on the cardiovascular complications that arise from OSA. Nevertheless, a detailed understanding of this association is lacking. This review examines recent advancements to clarify the link between the gut microbiome, OSA, and OSA-related CVDs, with a specific focus on hypertension, and also explores potential health advantages of adjuvant therapy that targets the gut microbiome in OSA.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":"6 4","pages":"118-130"},"PeriodicalIF":2.7,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2023-00153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140078501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ROCK1 deficiency preserves caveolar compartmentalization of signaling molecules and cell membrane integrity","authors":"Jianjian Shi,&nbsp;Lei Wei","doi":"10.1096/fba.2024-00015","DOIUrl":"https://doi.org/10.1096/fba.2024-00015","url":null,"abstract":"<p>In this study, we investigated the roles of ROCK1 in regulating structural and functional features of caveolae located at the cell membrane of cardiomyocytes, adipocytes, and mouse embryonic fibroblasts (MEFs) as well as related physiopathological effects. Caveolae are small bulb-shaped cell membrane invaginations, and their roles have been associated with disease conditions. One of the unique features of caveolae is that they are physically linked to the actin cytoskeleton that is well known to be regulated by RhoA/ROCKs pathway. In cardiomyocytes, we observed that ROCK1 deficiency is coincident with an increased caveolar density, clusters, and caveolar proteins including caveolin-1 and -3. In the mouse cardiomyopathy model with transgenic overexpressing Gαq in myocardium, we demonstrated the reduced caveolar density at cell membrane and reduced caveolar protein contents. Interestingly, coexisting ROCK1 deficiency in cardiomyocytes can rescue these defects and preserve caveolar compartmentalization of β-adrenergic signaling molecules including β1-adrenergic receptor and type V/VI adenylyl cyclase. In cardiomyocytes and adipocytes, we detected that ROCK1 deficiency increased insulin signaling with increased insulin receptor activation in caveolae. In MEFs, we identified that ROCK1 deficiency increased caveolar and total levels of caveolin-1 and cell membrane repair ability after mechanical or chemical disruptions. Together, these results demonstrate that ROCK1 can regulate caveolae plasticity and multiple functions including compartmentalization of signaling molecules and cell membrane repair following membrane disruption by mechanical force and oxidative damage. These findings provide possible molecular insights into the beneficial effects of ROCK1 deletion/inhibition in cardiomyocytes, adipocytes, and MEFs under certain diseased conditions.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":"6 3","pages":"85-102"},"PeriodicalIF":2.7,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2024-00015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140053252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of chaperone-mediated autophagy does not alter age-related bone loss in male mice","authors":"James A. Hendrixson,&nbsp;Alicen James,&nbsp;Nisreen S. Akel,&nbsp;Dominique J. Laster,&nbsp;Julie A. Crawford,&nbsp;Stuart B. Berryhill,&nbsp;Melda Onal","doi":"10.1096/fba.2023-00133","DOIUrl":"https://doi.org/10.1096/fba.2023-00133","url":null,"abstract":"<p>Chaperone-mediated autophagy (CMA) is a lysosome-dependent degradation pathway that eliminates proteins that are damaged, partially unfolded, or targeted for selective proteome remodeling. CMA contributes to several cellular processes, including stress response and proteostasis. Age-associated increase in cellular stressors and decrease in CMA contribute to pathologies associated with aging in various tissues. CMA contributes to bone homeostasis in young mice. An age-associated reduction in CMA was reported in osteoblast lineage cells; however, whether declining CMA contributes to skeletal aging is unknown. Herein we show that cellular stressors stimulate CMA in UAMS-32 osteoblastic cells. Moreover, the knockdown of an essential component of the CMA pathway, LAMP2A, sensitizes osteoblasts to cell death caused by DNA damage, ER stress, and oxidative stress. As elevations in these stressors are thought to contribute to age-related bone loss, we hypothesized that declining CMA contributes to the age-associated decline in bone formation by sensitizing osteoblast lineage cells to elevated stressors. To test this, we aged male CMA-deficient mice and controls up to 24 months of age and examined age-associated changes in bone mass and architecture. We showed that lack of CMA did not alter age-associated decline in bone mineral density as measured by dual x-ray absorptiometry (DXA). Moreover, microCT analysis performed at 24 months of age showed that vertebral cancellous bone volume, cortical thickness, and porosity of CMA-deficient and control mice were similar. Taken together, these results suggest that reduction of CMA does not contribute to age-related bone loss.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":"6 3","pages":"73-84"},"PeriodicalIF":2.7,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2023-00133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140053256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Club cell-specific telomere protection protein 1 (TPP1) protects against tobacco smoke-induced lung inflammation, xenobiotic metabolic dysregulation, and injurious responses","authors":"Thivanka Muthumalage,&nbsp;Chiara Goracci,&nbsp;Irfan Rahman","doi":"10.1096/fba.2023-00115","DOIUrl":"10.1096/fba.2023-00115","url":null,"abstract":"<p>Inhaling xenobiotics, such as tobacco smoke is a major risk factor for pulmonary diseases, e.g., COPD/emphysema, interstitial lung disease, and pre-invasive diseases. Shelterin complex or telosome provides telomeric end protection during replication. Telomere protection protein 1 (TPP1) is one of the main six subunits of the shelterin complex supporting the telomere stability and genomic integrity. Dysfunctional telomeres and shelterin complex are associated as a disease mechanism of tobacco smoke-induced pulmonary damage and disease processes. The airway epithelium is critical to maintaining respiratory homeostasis and is implicated in lung diseases. Club cells (also known as clara cells) play an essential role in the immune response, surfactant production, and metabolism. Disrupted shelterin complex may lead to dysregulated cellular function, DNA damage, and disease progression. However, it is unknown if the conditional removal of TPP1 from Club cells can induce lung disease pathogenesis caused by tobacco smoke exposure. In this study, conditional knockout of Club-cell specific TPP1 demonstrated the instability of other shelterin protein subunits, such as TRF1, dysregulation of cell cycle checkpoint proteins, p53 and downstream targets, and dysregulation of telomeric genes. This was associated with age-dependent senescence-associated genes, increased DNA damage, and upregulated RANTES/IL13/IL33 mediated lung inflammation and injury network by cigarette smoke (CS). These phenomena are also associated with alterations in cytochrome P450 and glutathione transferases, upregulated molecular pathways promoting lung lesions, bronchial neoplasms, and adenocarcinomas. These findings suggest a pivotal role of TPP1 in maintaining lung homeostasis and injurious responses in response to CS. Thus, these data TPP1 may have therapeutic value in alleviating telomere-related chronic lung diseases.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":"6 2","pages":"53-71"},"PeriodicalIF":2.7,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2023-00115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139622618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of air–liquid interface on cultured human intestinal epithelial cells","authors":"Akanksha Sabapaty,&nbsp;Po-Yu Lin,&nbsp;James C. Y. Dunn","doi":"10.1096/fba.2023-00132","DOIUrl":"10.1096/fba.2023-00132","url":null,"abstract":"<p>The intestinal epithelium is a dynamic barrier that allows the selective exchange of ions, hormones, proteins, and nutrients. To accomplish this, the intestinal epithelium adopts a highly columnar morphology which is partially lost in submerged culturing systems. To achieve this, small intestinal tissue samples were utilized to obtain human intestinal crypts to form enteroids. The Transwell system was subsequently employed to form a monolayer of cells that was cultured in either the submerged condition or the air–liquid Interface (ALI) condition. We found that the human intestinal monolayer under the ALI condition exhibited morphology more similar to the normal intestinal epithelium. F-actin localization and brush border formation were observed apically, and the integrity of the tight junctions was preserved in the ALI condition. Fewer apoptotic cells were observed in the ALI conditions as compared to the submerged conditions. The monolayer of cells expressed a higher level of secretory cell lineage genes in the ALI condition. The ALI condition positively contributes toward a more differentiated phenotype of epithelial cells. It serves as an amplifier that enhances the existing differentiation cue. The ALI system provides a more differentiated platform to study intestinal function compared to submerged conditions.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":"6 2","pages":"41-52"},"PeriodicalIF":2.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2023-00132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138952490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered microheterogeneity at several N-glycosylation sites in OPSCC in constant protein expression conditions","authors":"Amy Dickinson,&nbsp;Sakari Joenväärä,&nbsp;Tiialotta Tohmola,&nbsp;Jutta Renkonen,&nbsp;Petri Mattila,&nbsp;Timo Carpén,&nbsp;Antti Mäkitie,&nbsp;Suvi Silén","doi":"10.1096/fba.2023-00066","DOIUrl":"10.1096/fba.2023-00066","url":null,"abstract":"<p>Protein glycosylation responds sensitively to disease states. It is implicated in every hallmark of cancer and has recently started to be considered as a hallmark itself. Changes in N-glycosylation microheterogeneity are more dramatic than those of protein expression due to the non-template nature of protein glycosylation. This enables their potential use in serum-based diagnostics. Here, we perform glycopeptidomics on serum from patients with oropharyngeal squamous cell carcinoma (OPSCC), compared to controls and comparing between cancers based on etiology (human papilloma virus- positive or negative). Using MS2, we then targeted glycoforms, significantly different between the groups, to identify their glycopeptide compositions. Simultaneously we investigate the same serum proteins, comparing whether N-glycosylation changes reflect protein-level changes. Significant glycoforms were identified from proteins such as alpha-1-antitrypsin (SERPINA1), haptoglobin, and different immunoglobulins. SERPINA1 had glycovariance at 2 N-glycosylation sites, that were up to 35 times more abundant in even early-stage OPSCCs, despite minimal differences between SERPINA1 protein levels between groups. Some identified glycoforms' fold changes (FCs) were in line with serum protein level FCs, others were less abundant in early-stage cancers but with great variance in higher-stage cancers, such as on immunoglobulin heavy constant gamma 2, despite no change in protein levels. Such findings indicate that glycovariant analysis might be more beneficial than proteomic analysis, which is yet to be fruitful in the search for biomarkers. Highly sensitive glycopeptide changes could potentially be used in the future for cancer screening. Additionally, characterizing the glycopeptide changes in OPSCC is valuable in the search for potential therapeutic targets.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":"6 1","pages":"26-39"},"PeriodicalIF":2.7,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2023-00066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138974648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}