FASEB bioAdvances最新文献

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Medium-chain fatty acid receptor GPR84 deficiency leads to metabolic homeostasis dysfunction in mice fed high-fat diet 中链脂肪酸受体 GPR84 缺乏会导致高脂饮食小鼠代谢平衡失调
IF 2.5
FASEB bioAdvances Pub Date : 2024-10-17 DOI: 10.1096/fba.2024-00075
Akari Nishida, Ryuji Ohue-Kitano, Yuki Masujima, Hazuki Nonaka, Miki Igarashi, Takako Ikeda, Ikuo Kimura
{"title":"Medium-chain fatty acid receptor GPR84 deficiency leads to metabolic homeostasis dysfunction in mice fed high-fat diet","authors":"Akari Nishida,&nbsp;Ryuji Ohue-Kitano,&nbsp;Yuki Masujima,&nbsp;Hazuki Nonaka,&nbsp;Miki Igarashi,&nbsp;Takako Ikeda,&nbsp;Ikuo Kimura","doi":"10.1096/fba.2024-00075","DOIUrl":"https://doi.org/10.1096/fba.2024-00075","url":null,"abstract":"<p>Overconsumption of food, especially dietary fat, leads to metabolic disorders such as obesity and type 2 diabetes. Long-chain fatty acids, such as palmitoleate are recognized as the risk factors for these disorders owing to their high-energy content and lipotoxicity. In contrast, medium-chain fatty acids (MCFAs) metabolic benefits; however, their underlying molecular mechanisms remain unclear. GPR84 is an MCFA receptor, particularly for C10:0. Although evidence from in vitro experiments and oral administration of C10:0 in mice suggests that GPR84 is related to the metabolic benefits of MCFAs via glucose metabolism, its precise roles in vivo remain unclear. Therefore, the present study investigated whether GPR84 affects glucose metabolism and metabolic function using <i>Gpr84</i>-deficient mice. Although <i>Gpr84</i>-deficient mice were lean and had increased endogenous MCFAs under high-fat diet feeding conditions, they exhibited hyperglycemia and hyperlipidemia along with lower plasma insulin and glucagon-like peptide-1 (GLP-1) levels compared with wild-type mice. Medium-chain triglyceride (C10:0) intake suppressed obesity, and improved plasma glucose and lipid levels, and increased plasma GLP-1 levels in wild-type mice; however, these effects were partially attenuated in <i>Gpr84</i>-deficient mice. Our results indicate that long-term MCFA-mediated GPR84 activation improves the dysfunction of glucose and lipid homeostasis. Our findings may be instrumental for future studies on drug development with GPR84 as a potential target, thereby offering new avenues for the treatment of metabolic disorders like obesity and type 2 diabetes.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2024-00075","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142596184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
TMEM182 inhibits myocardial differentiation of human iPS cells by maintaining the activated state of Wnt/β-catenin signaling through an increase in ILK expression TMEM182 通过增加 ILK 的表达维持 Wnt/β-catenin 信号的激活状态,从而抑制人 iPS 细胞的心肌分化
IF 2.5
FASEB bioAdvances Pub Date : 2024-10-14 DOI: 10.1096/fba.2024-00086
Hirofumi Morihara, Shunichi Yokoe, Shigeo Wakabayashi, Shinji Takai
{"title":"TMEM182 inhibits myocardial differentiation of human iPS cells by maintaining the activated state of Wnt/β-catenin signaling through an increase in ILK expression","authors":"Hirofumi Morihara,&nbsp;Shunichi Yokoe,&nbsp;Shigeo Wakabayashi,&nbsp;Shinji Takai","doi":"10.1096/fba.2024-00086","DOIUrl":"https://doi.org/10.1096/fba.2024-00086","url":null,"abstract":"<p>Transmembrane protein 182 (TMEM182) is notably abundant in muscle and adipose tissue, but its role in the heart remains unknown. This study examined the contribution of TMEM182 in the differentiation of human induced pluripotent stem cells (hiPSCs) into cardiomyocytes. For this, we generated hiPSCs overexpressing TMEM182 in a doxycycline-inducible manner and induced their differentiation into cardiomyocytes. On Day 12 of differentiation, expression of the cardiomyocyte markers, <i>TNNT2</i> and <i>MYH6</i>, was significantly decreased in TMEM182-overexpressing cells. Additionally, we found that phosphorylation of GSK-3β (Ser9) and β-catenin (Ser552) was increased during TMEM182 overexpression, suggesting activation of Wnt/β-catenin signaling. We further focused on integrin-linked kinase (ILK) as the mechanism by which TMEM182 activates Wnt/β-catenin signaling. Evaluation showed that ILK expression was increased in cells overexpressing TMEM182. These results suggest that TMEM182 maintains Wnt/β-catenin signaling in an activated state after mesoderm formation by increasing ILK expression, thereby suppressing hiPSCs differentiation into cardiomyocytes.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2024-00086","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142596231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New role of calcium-binding fluorescent dye alizarin complexone in detecting permeability from articular cartilage to subchondral bone 钙结合荧光染料茜素络合酮在检测从关节软骨到软骨下骨的渗透性方面的新作用
IF 2.5
FASEB bioAdvances Pub Date : 2024-10-07 DOI: 10.1096/fba.2024-00103
Mingshu Cui, Mengcun Chen, Yanmei Yang, Hamza Akel, Bin Wang
{"title":"New role of calcium-binding fluorescent dye alizarin complexone in detecting permeability from articular cartilage to subchondral bone","authors":"Mingshu Cui,&nbsp;Mengcun Chen,&nbsp;Yanmei Yang,&nbsp;Hamza Akel,&nbsp;Bin Wang","doi":"10.1096/fba.2024-00103","DOIUrl":"https://doi.org/10.1096/fba.2024-00103","url":null,"abstract":"<p>Osteoarthritis (OA) is a chronic degenerative joint disorder characterized by the progressive deterioration of articular cartilage and concomitant alterations in subchondral bone architecture. However, the precise mechanisms underlying the initiation and progression of OA remains poorly understood. In the present study, we explored whether the calcification in the articular cartilage occurred in the early stage of mouse OA model, generated by the surgery destabilization of the medial meniscus (DMM), via the intra-articular injection of alizarin complexone due to its anionic nature for binding calcium-containing crystals. Although we did not observe the calcification in the articular cartilage of early stage of DMM mice, we unexpectedly identified alizarin complexone had the diffusion capacity for detecting the permeability from the articular cartilage to subchondral bone. Our data showed that the diffusion of alizarin complexone from the articular cartilage to calcified cartilage was greater in the early stage of DMM mice than that in sham controls. Additionally, we observed enhanced penetration of alizarin complexone through the periosteum in DMM mice compared to sham mice. In summary, we developed a novel imaging method that offers a valuable tool for further exploration of biochemical communication underlying OA development. Our findings provided new evidence that increased molecular interactions between the articular cartilage and subchondral bone is involved in the pathogenesis of OA progression.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2024-00103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142596364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Everything, everywhere, and all at once: A blueprint for supra-organization of core facilities 事无巨细,无处不在,一气呵成:核心设施超组织蓝图
IF 2.5
FASEB bioAdvances Pub Date : 2024-10-07 DOI: 10.1096/fba.2024-00094
Tsiona Elkayam Cohen, Robert Fluhr
{"title":"Everything, everywhere, and all at once: A blueprint for supra-organization of core facilities","authors":"Tsiona Elkayam Cohen,&nbsp;Robert Fluhr","doi":"10.1096/fba.2024-00094","DOIUrl":"https://doi.org/10.1096/fba.2024-00094","url":null,"abstract":"<p>Core facilities are crucial for cutting-edge scientific research in academic institutions, yet they place a significant financial burden on budgets. The viability of these facilities can be improved through cross-institutional collaborations, although initiating and sustaining such partnerships poses challenges. Insights from Israel's recent nationwide organization of core facilities could offer valuable lessons for fostering similar cooperation elsewhere. Despite the chronic shortfall in public research funding, Israeli research institutions were slow to fully embrace infrastructure sharing. This gap led to the creation of the Israel Research Core Facilities (IRCF) in 2022, which linked core facilities across the country through a bottom-up approach. IRCF facilitated the formation of numerous specialized nation-wide networks for intellectual exchange, and supported training workshops and meetings aimed at core technology providers. These initiatives serve dual purposes: they ensure the ongoing advancement of technological capabilities across facilities, regardless of their size or location, and they strengthen the commitment to the IRCF mission by motivating the maintenance of the IRCF database. As a result, a model of “capacity sharing” emerged, connecting all of Israel's core facility centers. This model enhances infrastructure use, supports strategic planning, and fosters growth. With over 450 core experts offering over 1100 scientific services consolidated into a publicly accessible database, IRCF supports research in universities, hospitals, government, and industry. This strategy could act as a model for creating regional core facility organizations to elevate research quality and ensure efficient infrastructure development.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2024-00094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epigenetic regulation of cardiovascular diseases induced by behavioral and environmental risk factors: Mechanistic, diagnostic, and therapeutic insights 行为和环境风险因素诱发心血管疾病的表观遗传调控:机理、诊断和治疗见解
IF 2.5
FASEB bioAdvances Pub Date : 2024-10-01 DOI: 10.1096/fba.2024-00080
Feifei Bi, Chen Gao, Hongchao Guo
{"title":"Epigenetic regulation of cardiovascular diseases induced by behavioral and environmental risk factors: Mechanistic, diagnostic, and therapeutic insights","authors":"Feifei Bi,&nbsp;Chen Gao,&nbsp;Hongchao Guo","doi":"10.1096/fba.2024-00080","DOIUrl":"https://doi.org/10.1096/fba.2024-00080","url":null,"abstract":"<p>Behavioral and environmental risk factors are critical in the development and progression of cardiovascular disease (CVD). Understanding the molecular mechanisms underlying these risk factors will offer valuable insights for targeted preventive and therapeutic strategies. Epigenetic modifications, including DNA methylation, histone modifications, chromatin remodeling, noncoding RNA (ncRNA) expression, and epitranscriptomic modifications, have emerged as key mediators connecting behavioral and environmental risk factors to CVD risk and progression. These epigenetic alterations can profoundly impact on cardiovascular health and susceptibility to CVD by influencing cellular processes, development, and disease risk over an individual's lifetime and potentially across generations. This review examines how behavioral and environmental risk factors affect CVD risk and health outcomes through epigenetic regulation. We review the epigenetic effects of major behavioral risk factors (such as smoking, alcohol consumption, physical inactivity, unhealthy diet, and obesity) and environmental risk factors (including air and noise pollution) in the context of CVD pathogenesis. Additionally, we explore epigenetic biomarkers, considering their role as causal or surrogate indicators, and discuss epigenetic therapeutics targeting the mechanisms through which these risk factors contribute to CVD. We also address future research directions and challenges in leveraging epigenetic insights to reduce the burden of CVD related to behavioral and environmental factors and improve public health outcomes. This review aims to provide a comprehensive understanding of behavioral and environmental epigenetics in CVD and offer valuable strategies for therapeutic intervention.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2024-00080","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-cell and spatial transcriptomics: Discovery of human placental development and disease 单细胞和空间转录组学:发现人类胎盘发育和疾病
IF 2.5
FASEB bioAdvances Pub Date : 2024-09-21 DOI: 10.1096/fba.2024-00133
Mi Tang, Liling Xiong, Jianghui Cai, Li Fan, Cheng Huang, Shimao Zhang, Ying Jin, Er-dan Luo, ShaSha Xing, Xiao Yang
{"title":"Single-cell and spatial transcriptomics: Discovery of human placental development and disease","authors":"Mi Tang,&nbsp;Liling Xiong,&nbsp;Jianghui Cai,&nbsp;Li Fan,&nbsp;Cheng Huang,&nbsp;Shimao Zhang,&nbsp;Ying Jin,&nbsp;Er-dan Luo,&nbsp;ShaSha Xing,&nbsp;Xiao Yang","doi":"10.1096/fba.2024-00133","DOIUrl":"https://doi.org/10.1096/fba.2024-00133","url":null,"abstract":"<p>The human placenta is a vital organ, encompassing many distinct cell types, that maintains the growth and development of the fetus and is essential for substance exchange, defense, synthesis, and immunity. Abnormalities in placental cells can lead to various pregnancy complications, but the mechanisms remain largely unclear. Single-cell and spatial transcriptomics technologies have been developed in recent years to demonstrate placental cell heterogeneity and spatial molecular localization. Here, we review and summarize the current literature, demonstrating these technologies and showing the heterogeneity of various placenta cells and cell–cell communication of normal human placenta, as well as placenta-related diseases, such as preeclampsia, gestational diabetes mellitus, advanced maternal age, recurrent pregnancy loss, and placenta accreta spectrum disorders. Meanwhile, current weaknesses and future direction were discussed.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2024-00133","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gut microbiota and autoimmune diseases: Insights from Mendelian randomization 肠道微生物群与自身免疫性疾病:孟德尔随机化的启示
IF 2.5
FASEB bioAdvances Pub Date : 2024-09-21 DOI: 10.1096/fba.2024-00037
Fangxiang Mu, Gusbakti Rusip, Florenly Florenly
{"title":"Gut microbiota and autoimmune diseases: Insights from Mendelian randomization","authors":"Fangxiang Mu,&nbsp;Gusbakti Rusip,&nbsp;Florenly Florenly","doi":"10.1096/fba.2024-00037","DOIUrl":"https://doi.org/10.1096/fba.2024-00037","url":null,"abstract":"<p>In recent years, the scientific community has shown interest in the role of gut microbiota in the development of autoimmune diseases (AID). Although observational studies have revealed significant associations between gut microbiota and AID like rheumatoid arthritis, systemic lupus erythematosus, and multiple sclerosis, these connections do not necessarily imply causality. Mendelian randomization (MR) approach has been extensively employed to investigate the causal relationship. Relevant MR study findings indicate that a reduction in beneficial microbial populations, particularly <i>Bifidobacterium</i> and <i>Lactobacillus</i>, and an increase in potential pathogenic microbes, is correlated with an elevated AID risk. Given the innovative potential of MR in unraveling the etiopathogenesis of AIDs, this article offers an overview of this methodological approach and its recent applications in AID research.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2024-00037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mitochondrial functional impairment in ARL3-mutation related rod-cone dystrophy 与 ARL3 基因突变相关的视杆细胞色素营养不良症中的线粒体功能障碍
IF 2.5
FASEB bioAdvances Pub Date : 2024-09-09 DOI: 10.1096/fba.2023-00138
Xiaoli Zhang, Shun Yao, Lujia Zhang, Beisi Zhang, Mingzhu Yang, Qingge Guo, Jin Xu, Zhongfeng Wang, Bo Lei, Xiuxiu Jin
{"title":"Mitochondrial functional impairment in ARL3-mutation related rod-cone dystrophy","authors":"Xiaoli Zhang,&nbsp;Shun Yao,&nbsp;Lujia Zhang,&nbsp;Beisi Zhang,&nbsp;Mingzhu Yang,&nbsp;Qingge Guo,&nbsp;Jin Xu,&nbsp;Zhongfeng Wang,&nbsp;Bo Lei,&nbsp;Xiuxiu Jin","doi":"10.1096/fba.2023-00138","DOIUrl":"https://doi.org/10.1096/fba.2023-00138","url":null,"abstract":"<p>Mitochondria are vital for retinal cell function and survival, and there is growing evidence linking mitochondrial dysfunction to retinal degenerations. Although <i>ARL3</i> mutations have been linked to multiple forms of retinal degeneration, the relationship between ARL3 and mitochondria remains unexplored. Herein, we investigated the effects of <i>ARL3</i><sup><i>T31A</i></sup>, <i>ARL3</i><sup><i>C118F</i></sup>, and <i>ARL3</i><sup><i>T31A/C118F</i></sup> mutations on mitochondrial function in fibroblasts obtained from patients with ARL3-related rod-cone dystrophy. Our findings revealed that these mutations led to a decrease in mitochondrial respiration, an increase in the accumulation mitochondrial reactive oxygen species (ROS), and induction of apoptosis in fibroblasts. Additionally, we conducted a comparative analysis of the effects of ARL3<sup>T31A</sup>, ARL3<sup>C118F</sup>, and ARL3<sup>T31A/C118F</sup> proteins on mitochondria in ARPE-19 cells. Results showed that ARL3<sup>T31A</sup> and ARL3<sup>T31A/C118F</sup> not only affected mitochondrial function but also induced apoptosis in ARPE-19 cells. Conversely, ARL3<sup>C118F</sup> primarily influenced cell apoptosis with minimal effects on mitochondrial function in ARPE-19 cells. Transcriptome analysis further suggested the involvement of respiratory electron transport, response to ROS, and apoptotic signaling pathways in ARL3<sup>T31A/C118F</sup> cells. Our study demonstrated that <i>ARL3</i>-related mutations play a significant role in the diversity of mitochondrial function, providing novel insights into the functional analysis of <i>ARL3</i>-related mutations.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1096/fba.2023-00138","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142595658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
AAV-mouse DNase I sustains long-term DNase I expression in vivo and suppresses breast cancer metastasis AAV-小鼠 DNase I 可维持体内 DNase I 的长期表达并抑制乳腺癌转移。
IF 2.5
FASEB bioAdvances Pub Date : 2024-08-30 DOI: 10.1096/fba.2024-00114
Melanie Herre, Kalyani Vemuri, Jessica Cedervall, Stefanie Nissl, Falk Saupe, Jacob Micallef, Henrik Lindman, Casey A. Maguire, George Tetz, Victor Tetz, Anna-Karin Olsson
{"title":"AAV-mouse DNase I sustains long-term DNase I expression in vivo and suppresses breast cancer metastasis","authors":"Melanie Herre,&nbsp;Kalyani Vemuri,&nbsp;Jessica Cedervall,&nbsp;Stefanie Nissl,&nbsp;Falk Saupe,&nbsp;Jacob Micallef,&nbsp;Henrik Lindman,&nbsp;Casey A. Maguire,&nbsp;George Tetz,&nbsp;Victor Tetz,&nbsp;Anna-Karin Olsson","doi":"10.1096/fba.2024-00114","DOIUrl":"10.1096/fba.2024-00114","url":null,"abstract":"<p>Neutrophil extracellular traps (NETs) have been implicated in the pathology of various inflammatory conditions. In cancer, NETs have been demonstrated to induce systemic inflammation, impair peripheral vessel and organ function and promote metastasis. Here we show that the plasma level of NETs is significantly higher in patients with metastatic breast cancer compared to those with local disease, or those that were considered cured at a 5-year follow-up, confirming NETs as interesting therapeutic targets in metastatic breast cancer. Administration of DNase I is one strategy to eliminate NETs but long-term treatment requires repeated injections and species-specific versions of the enzyme. To enhance administration and therapeutic efficacy, we have developed an adeno-associated virus (AAV) vector system for delivery of murine DNase I and addressed its potential to counteract cancer-associated pathology in the murine MMTV-PyMT model for metastatic mammary carcinoma. The AAV vector is comprised of capsid KP1 and an expression cassette encoding hyperactive murine DNase I (AAV-mDNase I) under the control of a liver-specific promotor. This AAV-mDNase I vector could support elevated expression and serum activity of murine DNase I over at least 8 months. Neutrophil Gelatinase-Associated Lipocalin (NGAL), a biomarker for kidney hypoperfusion that is upregulated in urine from MMTV-PyMT mice, was suppressed in mice receiving AAV-mDNase I compared to an AAV-null control group. Furthermore, the proportion of mice that developed lung metastasis was reduced in the AAV-mDNase I group. Altogether, our data indicate that AAV-mDNase I has the potential to reduce cancer-associated impairment of renal function and development of metastasis. We conclude that AAV-mDNase I could represent a promising therapeutic strategy in metastatic breast cancer.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11452440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An unidentified yet notable modification on INa and IK(DR) caused by ramelteon 雷美替胺对 I Na 和 I K (DR) 造成的一种尚未查明但却显著的改变。
IF 2.5
FASEB bioAdvances Pub Date : 2024-08-28 DOI: 10.1096/fba.2024-00008
Po-Ming Wu, Yi-Fang Tu, Hsin-Yen Cho, Meng-Cheng Yu, Yen-Hsien Wu, Sheng-Nan Wu
{"title":"An unidentified yet notable modification on INa and IK(DR) caused by ramelteon","authors":"Po-Ming Wu,&nbsp;Yi-Fang Tu,&nbsp;Hsin-Yen Cho,&nbsp;Meng-Cheng Yu,&nbsp;Yen-Hsien Wu,&nbsp;Sheng-Nan Wu","doi":"10.1096/fba.2024-00008","DOIUrl":"10.1096/fba.2024-00008","url":null,"abstract":"<p>Despite advancement in anti-seizure medications, 30% of patients continue to experience recurrent seizures. Previous data indicated the antiepileptic properties of melatonin and its agonists in several animal models. However, the underlying mechanisms of melatonin and its agonists on cellular excitability remain poorly understood. In this study, we demonstrated the electrophysiological changes of two main kinds of ion channels that are responsible for hyperexcitability of neurons after introduction of melatonin agonists- ramelteon (RAM). In Neuro-2a cells, the amplitude of voltage-gated Na<sup>+</sup> (<i>I</i><sub>Na</sub>) and delayed-rectifier K<sup>+</sup> currents (<i>I</i><sub>K (DR)</sub>) could be suppressed under RAM. The IC<sub>50</sub> values of 8.7 and 2.9 μM, respectively. RAM also diminished the magnitude of window Na<sup>+</sup> current (<i>I</i><sub>Na (W)</sub>) elicited by short ascending ramp voltage, with unchanged the overall steady-state current–voltage relationship. The decaying time course of <i>I</i><sub>Na</sub> during a train of depolarizing pulses arose upon the exposure to RAM. The conditioning train protocol which blocked <i>I</i><sub>Na</sub> fitted the recovery time course into two exponential processes and increased the fast and slow time constant of recovery the presence of RAM. In pituitary tumor (GH<sub>3</sub>) cells, <i>I</i><sub>Na</sub> amplitude was also effectively suppressed by the RAM. In addition, GH3-cells exposure to RAM decreased the firing frequency of spontaneous action potentials observed under current-clamp conditions. As a result, the RAM-mediated effect on INa was closely associated with its ability to decrease spontaneous action potentials. Collectively, we found the direct attenuation of <i>I</i><sub>Na</sub> and <i>I</i><sub>K (DR)</sub> caused by RAM besides the agonistic action on melatonin receptors, which could partially explain its anti-seizure activity.</p>","PeriodicalId":12093,"journal":{"name":"FASEB bioAdvances","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11452446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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