Experientia最新文献_第5页

Effects of Freund's complete adjuvant on the diurnal rhythms of neuroendocrine processes and ornithine decarboxylase activity in various tissues of male rats. 弗氏完全佐剂对雄性大鼠各组织神经内分泌过程昼夜节律及鸟氨酸脱羧酶活性的影响。

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938878

M Neidhart

{"title":"Effects of Freund's complete adjuvant on the diurnal rhythms of neuroendocrine processes and ornithine decarboxylase activity in various tissues of male rats.","authors":"M Neidhart","doi":"10.1007/BF01938878","DOIUrl":"https://doi.org/10.1007/BF01938878","url":null,"abstract":"The aim of this study was to investigate the effects of Freund's complete adjuvant (FCA) on the diurnal rhythms of hormonal parameters in serum and ornithine decarboxylase (ODC) activity in various tissues of male rats. On days 1-2 after FCA, increase of ODC activity (used to evaluate the level of activation) was observed in the hypothalamus, pituitary gland, adrenal medulla, adrenal cortex, liver and lymphoid tissues, while the ODC activity in the kidney was reduced. This was accompanied by an increase in serum corticosterone. On days 3-4 after FCA, ODC activity remained elevated in the pituitary gland, liver and lymphoid tissues, while the ODC activity in the testes and pancreas was reduced; kidney ODC activity returned to baseline. This was associated with increased serum levels of prolactin (Prl) and luteinizing hormone, but decreased growth hormone, testosterone and insulin. The increase in ODC activity in the thymus, as well as the reduced ODC activity in the testes and kidney, can be obtained with paraffin. Furthermore, bromocryptine microcapsules (CBLA) reduced the FCA-induced increase of ODC activity in the pituitary gland, liver and lymphoid tissues (days 3-4) but did not affect the changes in other tissues. The increase in ODC activity in the pituitary gland, liver and lymphoid tissues is specific for FCA. A role for Prl in the induction of ODC in liver and lymphoid tissues is suggested by the fact that CBLA suppresses this enhancement.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 9","pages":"900-8"},"PeriodicalIF":0.0,"publicationDate":"1996-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01938878","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19809064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Splenic B-cell activation in lipopolysaccharide-non-responsive C3H/HeJ mice by lipopolysaccharide of Porphyromonas gingivalis. 牙龈卟啉单胞菌脂多糖对脂多糖无反应C3H/HeJ小鼠脾b细胞的激活作用。

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938879

H Shimauchi, T Ogawa, H Uchida, J Yoshida, H Ogoh, T Nozaki, H Okada

引用次数: 11

Effect of a dipeptide inhibiting ubiquitin-mediated protein degradation nerve-dependent limb regeneration in the newt. 抑制泛素介导的蛋白质降解的二肽对蝾螈神经依赖性肢体再生的影响。

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938871

C H Taban, H Hondermarck, R A Bradshaw, B Biolly

{"title":"Effect of a dipeptide inhibiting ubiquitin-mediated protein degradation nerve-dependent limb regeneration in the newt.","authors":"C H Taban, H Hondermarck, R A Bradshaw, B Biolly","doi":"10.1007/BF01938871","DOIUrl":"https://doi.org/10.1007/BF01938871","url":null,"abstract":"The dipeptide Leu-Ala, which inhibits ubiquitin-mediated protein degradation, has been shown to act in vitro as an inhibitor of neurite outgrowth of PC12 cells (Hondermarck et al. [1992] Biochem. Biophys. Res. Commun. 189:280). Using agarose beads as vehicles, we tested, in vivo, the effect of this dipeptide (and the inactive inverse, Ala-Leu, as a control) on limb regeneration in the newt (Triturus cristatus), a nerve-dependent developmental process. Leu-Ala inhibited the growth of mid-bud blastemas without altering blastema differentiation, while Ala-Leu had no effect. Cytological observations of dipeptide-treated blastemas using Bodian staining or neurofilament antibodies showed that all the blastema tissues were unmodified except with regard to innervation. Leu-Ala-treated blastemas were devoid of nerve fibers in the epidermal cap, while the mesenchyme distal to the dipeptide impregnated bead exhibited fewer nerve fibers than did Ala-Leu-treated blastemas, which were similar to the control nontreated blastemas. Thus, Leu-Ala, in reducing blastema innervation, inhibits its growth in the same manner as surgical denervation.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 9","pages":"865-70"},"PeriodicalIF":0.0,"publicationDate":"1996-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01938871","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19888902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Inhibition mechanisms of HIV-1, Mo-MuLV and AMV reverse transcriptases by Kelletinin A from Buccinulum corneum. 角膜Kelletinin A抑制HIV-1、Mo-MuLV和AMV逆转录酶的机制

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923995

P Orlando, G Strazzullo, F Carretta, M De Falco, P Grippo

{"title":"Inhibition mechanisms of HIV-1, Mo-MuLV and AMV reverse transcriptases by Kelletinin A from Buccinulum corneum.","authors":"P Orlando, G Strazzullo, F Carretta, M De Falco, P Grippo","doi":"10.1007/BF01923995","DOIUrl":"https://doi.org/10.1007/BF01923995","url":null,"abstract":"Kelletinin A [ribity pentakis (p-hydroxybenzoate)] (KA), an inhibitor of HTLV-1 replication isolated from Buccinulum corneum, showed a noncompetitive inhibitory activity with respect to the template primer and to dTTP in the poly(rA).oligo(dT)12-18-directed reaction of HIV-1, Mo-MuLV and AMV reverse transcriptases (RT). Analysis of natural and synthetic KA-related compounds showed that the inhibitory activity was strictly related to the structural peculiarities of the molecule. In the presence of DNA as template primer the inhibition mechanism was drastically modified: HIV-1 RT activity was stimulated by low concentrations of KA and was inhibited by increasing the concentration of the compound, while Mo-MuLV and AMV activities were irreversibly inhibited by the formation of a non-reactive complex. The RNase H activities of these RTs were not affected by KA. The results of this study suggest a different mechanism of interaction of Kelletinins with HIV-1 RT compared with other non-nucleoside inhibitors. A possible use of these drugs in combination therapy and in the design of structure-based reverse transcriptase inhibitors is discussed.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 8","pages":"812-7"},"PeriodicalIF":0.0,"publicationDate":"1996-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01923995","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19746651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Glucose formation from methylglyoxal in hepatocytes from streptozotocin-induced diabetic mice: the effect of insulin. 链脲佐菌素诱导的糖尿病小鼠肝细胞中甲基乙二醛生成葡萄糖:胰岛素的影响。

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923997

M P Kalapos, P Riba, T Garzo, J Mandl

引用次数: 7

Investigations of receptor-mediated phagocytosis by hormone-induced (imprinted) Tetrahymena pyriformis. 激素诱导(印迹)梨状四膜虫受体介导吞噬的研究。

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923987

P Kovács, C A Sundermann, G Csaba

引用次数: 11

Isolation of type II epithelial cells from rabbit fetal lungs by adherence on an IgG-coated surface. 兔胎肺ⅱ型上皮细胞在igg包被表面的粘附分离。

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923992

A Gupta, S Kaushal, S Majumdar, S N Sanyal

引用次数: 0

Synthesis of a ubiquitously present new HSP60 family protein is enhanced by heat shock only in the Malpighian tubules of Drosophila. 一种普遍存在的新的HSP60家族蛋白的合成仅在果蝇的马氏小管中被热休克增强。

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923984

S C Lakhotia, B N Singh

引用次数: 10

Lack of tolerance development to tumor necrosis factor alpha inside the central nervous system. 中枢神经系统对肿瘤坏死因子缺乏耐受性。

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923988

J M Goldbach, J Roth, B Störr, E Zeisberger

引用次数: 1

Induction of vanadium accumulation and nuclear sequestration causing cell suicide in human Chang liver cells. 在人肝细胞中诱导钒积累和核封存导致细胞自杀。

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923989

K H Sit, R Paramanantham, B H Bay, K P Wong, P Thong, F Watt

{"title":"Induction of vanadium accumulation and nuclear sequestration causing cell suicide in human Chang liver cells.","authors":"K H Sit, R Paramanantham, B H Bay, K P Wong, P Thong, F Watt","doi":"10.1007/BF01923989","DOIUrl":"https://doi.org/10.1007/BF01923989","url":null,"abstract":"Very little is known about the modulation of vanadium accumulation in cells, although this ultratrace element has long been seen as an essential nutrient in lower life forms, but not necessarily in humans where factors modulating cellular uptake of vanadium seem unclear. Using nuclear microscopy, which is capable of the direct evaluation of free and bound (total) elemental concentrations of single cells we show here that an NH4Cl acidification prepulse causes distinctive accumulation of vanadium (free and bound) in human Chang liver cells, concentrating particularly in the nucleus. Vanadium loaded with acidification but leaked away with realkalinization, suggests proton-dependent loading. Vanadyl(4), the oxidative state of intracellular vanadium ions, is known to be a potent source of hydroxyl free radicals (OH). The high oxidative state of nuclei after induction of vanadyl(4) loading was shown by the redox indicator methylene blue, suggesting direct oxidative damage to nuclear DNA. Flow cytometric evaluation of cell cycle phase-specific DNA composition showed degradation of both 2N and 4N DNA phases in G1, S and G2/M cell cycle profiles to a solitary IN DNA peak, in a dose-dependent manner, effective from micromolar vanadyl(4) levels. This trend was reproduced with microccocal nuclease digestion in a time response, supporting the notion of DNA fragmentation effects. Several other approaches confirmed fragmentation occurring in virtually all cells after 4mM V(4) loading. Ultrastructural profiles showed various stages of autophagic autodigestion and well defined plasma membrane outlines, consistent with programmed cell death but not with necrotic cell death. Direct intranuclear oxidative damage seemed associated with the induction of mass suicide in these human Chang liver cells following vanadium loading and nuclear sequestration.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 8","pages":"778-85"},"PeriodicalIF":0.0,"publicationDate":"1996-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01923989","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19746761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8