Experientia最新文献_第4页

The action of the peptidoleukotriene LTD4 on intracellular calcium in rat mesangial cells. 肽多溴三烯LTD4对大鼠系膜细胞内钙的作用。

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938870

M Ochsner

引用次数: 2

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938876

M Salehi, M A Hodgkins, B J Merry, M H Goyns

{"title":"Age-related changes in gene expression in the rat brain revealed by differential display.","authors":"M Salehi, M A Hodgkins, B J Merry, M H Goyns","doi":"10.1007/BF01938876","DOIUrl":"https://doi.org/10.1007/BF01938876","url":null,"abstract":"We have used the polymerase chain reaction (PCR)-based technique of differential display to analyse changes in gene expression during ageing of the rat brain. In this approach we have compared three young adult (6 months) with three old adult (20 months) animals. RNA preparations from the homogenised brains were subjected to reverse transcriptase (RT)-PCR using 36 different combinations of primer pairs. Any PCR product which was consistently found to be more prominent in the three young brains compared to the three old brains, and vice versa, was scored as potentially representing a gene which was differentially expressed during the ageing of this tissue. Out of a possible 2000+ PCR products we identified 44 that might represent genes that exhibit differential expression during ageing of the rat brain. An initial screen of these fragments, by Southern-blotting the PCR products and hybridising them with cDNA probes derived from either young or old brain RNA preparations, indicated that 40% of them represented genes that were differentially expressed. This approach is likely to prove invaluable for identifying cohorts of genes that show differential expression during the ageing process.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 9","pages":"888-91"},"PeriodicalIF":0.0,"publicationDate":"1996-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01938876","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19809062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Synergism between long-acting bromocryptine microcapsules and cyclosporine A in the prevention of various autoimmune diseases in rats. 长效溴隐碱微胶囊与环孢素A预防大鼠多种自身免疫性疾病的协同作用

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938877

M Neidhart

{"title":"Synergism between long-acting bromocryptine microcapsules and cyclosporine A in the prevention of various autoimmune diseases in rats.","authors":"M Neidhart","doi":"10.1007/BF01938877","DOIUrl":"https://doi.org/10.1007/BF01938877","url":null,"abstract":"Pre-treatment of male Sprague-Dawley rats with long-acting bromocryptine microcapsules (CBLA) significantly inhibited the arthritic response to Freund's complete adjuvant and reduced weight loss, thymolysis, splenomegaly and leukocytosis. In the prevention of adjuvant arthritis (AA), the combination of CBLA plus sub-optimal doses of cyclosporine A (CsA) was more efficient than either of the drugs alone. Sub-optimal doses of CsA were 0.1 and 1.0 mg/kg/day s.c. for 5 days. Furthermore, CBLA alone did not decrease the incidence of experimental allergic uveitis (EAU) in the male Lewis rats. Low-dose CsA reduced the incidence of uveitis by 50%, and with the addition of CBLA, 100% of rats were protected. Low-dose CsA was 2 mg/kg/day i.m. for 14 days. Long-term treatment of male Sprague-Dawley rats with CBLA alone reduced the incidence and severity of spontaneous autoimmune periarteritis nodosa (PN) in a dose-dependent manner; CsA was less potent than CBLA, and only additive effects were obtained. Finally, for the prevention of spontaneous autoimmune insulin-dependent diabetes (DM), the administration of CBLA did not improve the effect of a low-dose CsA in male BB rats. Nevertheless, a delay in onset of DM could be achieved. A sequential therapy using CsA plus CBLA clearly showed beneficial effects. The dose of CsA was 10 mg/kg p.o. 6 days/week for 21 weeks. Compared with Sprague-Dawley or Lewis male rats, BB male rats showed only weak prolactin suppression after the same doses of CBLA. It is suggested that the use of CBLA may be particularly beneficial in autoimmune disorders. The effectiveness of the combination therapy CBLA plus CsA, however, was dependent on the model considered. Various factors could play a role: (1) the different ways of administering CsA (s.c. in AA, i.m. in EAU and PN, oral in DM); (2) strain-dependency in the capacity of CBLA to suppress Prl secretion; and (3) at least in the BB rats, the transient increase of CsA bioavailibility which was possibly induced by CBLA.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 9","pages":"892-9"},"PeriodicalIF":0.0,"publicationDate":"1996-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01938877","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19809063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Freeze-induced shrinkage of individual cells and cell-to-cell propagation of intracellular ice in cell chains from salivary glands. 唾液腺细胞链中单个细胞的冷冻诱导收缩和细胞间冰的细胞间增殖。

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938868

W K Berger, B Uhrík

{"title":"Freeze-induced shrinkage of individual cells and cell-to-cell propagation of intracellular ice in cell chains from salivary glands.","authors":"W K Berger, B Uhrík","doi":"10.1007/BF01938868","DOIUrl":"https://doi.org/10.1007/BF01938868","url":null,"abstract":"The formation of intracellular ice (IIF), usually a lethal event to be avoided when cryopreserving cells, should, however, be enforced during the cryosurgical destruction of tumour cells. IIF has been investigated so far only in single cells in suspension. Because cells in tissues cannot be successfully cryopreserved, in contrast to single cells in suspension, the mechanism of IIF in tissues may depend on factors that facilitate IIF. We studied IIF in cell strands from salivary glands, which represent a simple form of a tissue. Their cells are connected by channels responsible for intercellular communication. A substantial fraction of cell dehydration during freezing occurs before cells are encapsulated by ice, and the degree of this pre-ice-front shrinkage appears to influence IIF. In strands with coupled cells IIF spread from one cell to adjacent cells in a sequential manner with short delays (200-300 ms), suggesting cell-to-cell propagation via intercellular channels. In strands pretreated with decoupling agents (dinitrophenol, heptanol), sequential IIF was absent. Instead, formation of ice was random, with longer and variable delays between consecutive darkenings indicating IIF. Results suggest that the mechanism of IIF spread, and consequently the degree of cryodamage in tissue, can be influenced by the presence of intercellular channels (gap junctions).","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 9","pages":"843-50"},"PeriodicalIF":0.0,"publicationDate":"1996-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01938868","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19809278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

Enzymatic formation of potential anticancer and antiviral inosine analogues. 潜在的抗癌和抗病毒肌苷类似物的酶促形成。

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938874

B Sheid, E Gaetjens, S T Chung, L M Lerner

引用次数: 2

The modulus of elasticity of lobster aorta microfibrils. 龙虾主动脉微纤维的弹性模量。

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938880

C J McConnell, G M Wright, M E DeMont

引用次数: 19

Bombyxin gene expression in tissues other than brain detected by reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization. 逆转录聚合酶链反应(RT-PCR)和原位杂交技术检测家蚕素基因在脑外组织中的表达。

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938875

M Iwami, A Tanaka, N Hano, S Sakurai

引用次数: 35

(-)-Epigallocatechin gallate, a polyphenolic tea antioxidant, inhibits peroxynitrite-mediated formation of 8-oxodeoxyguanosine and 3-nitrotyrosine. (-)-表没食子儿茶素没食子酸酯，一种多酚类茶抗氧化剂，抑制过氧亚硝酸盐介导的8-氧脱氧鸟苷和3-硝基酪氨酸的形成。

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938881

E S Fiala, R S Sodum, M Bhattacharya, H Li

引用次数: 34

Critical amino-terminal segments in insertion of rat liver cytochrome P450 3A1 into the endoplasmic reticulum membrane. 大鼠肝细胞色素P450 3A1插入内质网膜的关键氨基末端。

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938869

P J Van den Broek, M Barroso, M C Lechner

引用次数: 8

Overexpression of copper-zinc superoxide dismutase in trisomy 21. 铜锌超氧化物歧化酶在21三体中的过表达。

Experientia Pub Date : 1996-09-15 DOI: 10.1007/BF01938872

R De La Torre, A Casado, E López-Fernández, D Carrascosa, V Ramírez, J Sáez

{"title":"Overexpression of copper-zinc superoxide dismutase in trisomy 21.","authors":"R De La Torre, A Casado, E López-Fernández, D Carrascosa, V Ramírez, J Sáez","doi":"10.1007/BF01938872","DOIUrl":"https://doi.org/10.1007/BF01938872","url":null,"abstract":"Down's syndrome (DS), the most frequent of congenital birth defects, results from the trisomy of chromosome 21 in all cells of affected patients. This disease is characterized by developmental anomalies, mental retardation and features of rapid aging, particularly in the brain, where the occurrence of Alzheimer's disease is observed in trisomy 21 patients over the age of 35. Copper-zinc superoxide dismutase (CuZnSOD) is one of the proteins encoded by chromosome 21 (21q22.1). As a consequence of gene dosage excess, CuZnSOD activity is increased by 50% in all DS tissues. This work reports the SOD activity of a population of DS patients with complete trisomy 21, partial trisomy 21, translocations and mosaicism, in order to confirm the gene dosage effect of SOD on the clinical features of DS, and to help to establish which is the critical region of chromosome 21 in DS. CuZnSOD was measured in red blood cells using the Minami and Yoshikawa method. In the population with complete trisomy 21, SOD activity was increased by 42%; in the population with partial trisomy 21, translocations and mosaicism, SOD activity was normal. In the population diagnosed as DS, but not karyotyped, SOD activity was increased by 28%. No differences between sexes or among ages were found. We conclude that the 21q22.1 segment is not the critical region responsible for DS, as we have found normal SOD activity in patients with the clinical features of DS.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 9","pages":"871-3"},"PeriodicalIF":0.0,"publicationDate":"1996-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01938872","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19809281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46