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Heat shock enhances thermotolerance of infective juvenile insect-parasitic nematodes Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae). 热休克增强侵染性虫寄生线虫幼虫的耐热性。
Experientia Pub Date : 1996-07-15 DOI: 10.1007/BF01925583
S Selvan, P S Grewal, T Leustek, R Gaugler
{"title":"Heat shock enhances thermotolerance of infective juvenile insect-parasitic nematodes Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae).","authors":"S Selvan,&nbsp;P S Grewal,&nbsp;T Leustek,&nbsp;R Gaugler","doi":"10.1007/BF01925583","DOIUrl":"https://doi.org/10.1007/BF01925583","url":null,"abstract":"<p><p>Insect-parasitic nematodes possess many of the attributes of ideal biological control agents, but intolerance to extreme temperatures can restrict their use. We examined whether heat-shock treatments could improve nematode survival and infectivity at temperatures that normally inhibit their activity (35 and 40 degrees C). Nematodes exposed to a sub-lethal temperature (35 degrees C) for 3 h with a latency period of 1-2 h at 25 degrees C killed insects at 35 and 40 degrees C. Correlative evidence was obtained between increased thermotolerance and the synthesis of 70-kDa heat-shock proteins (hsps). These results provide the first evidence of hsp synthesis in the development of thermotolerance and biological activity in the non-feeding, developmentally arrested, infective juvenile nematodes.</p>","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 7","pages":"727-30"},"PeriodicalIF":0.0,"publicationDate":"1996-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01925583","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19672481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Immunocytochemical reaction of a haemocyanin antibody in the midgut gland of Nautilus (Cephalopoda, Tetrabranchiata). 鹦鹉螺(头足目,四鳃目)中肠腺中血色素抗体的免疫细胞化学反应。
Experientia Pub Date : 1996-06-15 DOI: 10.1007/BF01969726
P Ruth, W Blum, J Bille
{"title":"Immunocytochemical reaction of a haemocyanin antibody in the midgut gland of Nautilus (Cephalopoda, Tetrabranchiata).","authors":"P Ruth,&nbsp;W Blum,&nbsp;J Bille","doi":"10.1007/BF01969726","DOIUrl":"https://doi.org/10.1007/BF01969726","url":null,"abstract":"<p><p>The branchial gland of the dibranchiate cephalopods is described as the site of haemocyanin synthesis. Because there is no equivalent to this organ in tetrabranchiate cephalopods the localization of haemocyanin synthesis remained unknown for a long time. In this study we could confirm the conclusions from preliminary investigations concerning the copper content of the midgut gland of Nautilus, which gave the first indications for a possible localization of haemocyanin synthesis in this organ. We developed a polyclonal antibody against Nautilus haemocyanin, tested its specificity, and used it on ultra-thin sections of the tissue of the midgut gland. It could be shown that there is a clear immunogold precipitation only on the triangular basal cells in the terminal alveoli. All the other types of cell in this organ were free of any immunoreactivity. It can be supposed that the triangular basal cells in the terminal alveoli of the midgut gland are the sites of haemocyanin synthesis in Nautilus.</p>","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 6","pages":"549-53"},"PeriodicalIF":0.0,"publicationDate":"1996-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01969726","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19672098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Acclimatization effect on the evening fall in core temperature under the influence of two types of clothing. 两种服装对夜间体温下降的适应效应。
Experientia Pub Date : 1996-06-15 DOI: 10.1007/BF01969740
X Li, H Tokura
{"title":"Acclimatization effect on the evening fall in core temperature under the influence of two types of clothing.","authors":"X Li,&nbsp;H Tokura","doi":"10.1007/BF01969740","DOIUrl":"https://doi.org/10.1007/BF01969740","url":null,"abstract":"<p><p>This paper reports the effect of acclimatization on the evening fall in core temperature under the influence of two different types of clothing. Two groups of subjects dressed in either knee-length skirts or full trousers during the daytime for the three months from April to June. To compare the circadian rhythm of core temperature, the experiments were carried out before and after the three month program of acclimatization. It was found that the subjects who had worn knee-length skirts showed lower rectal temperatures during the nighttime and a bigger amplitude of circadian rhythm in July than in March.</p>","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 6","pages":"613-5"},"PeriodicalIF":0.0,"publicationDate":"1996-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01969740","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19671348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Resting energy expenditure in patients with cirrhosis of the liver measured by indirect calorimetry, anthropometry and bioelectrical impedance analysis. 用间接量热法、人体测量法和生物电阻抗分析法测量肝硬化患者静息能量消耗。
Experientia Pub Date : 1996-06-15 DOI: 10.1007/BF01969735
M Waluga, B Zahorska-Markiewicz, M Janusz, Z Słabiak, A Chełmicka
{"title":"Resting energy expenditure in patients with cirrhosis of the liver measured by indirect calorimetry, anthropometry and bioelectrical impedance analysis.","authors":"M Waluga,&nbsp;B Zahorska-Markiewicz,&nbsp;M Janusz,&nbsp;Z Słabiak,&nbsp;A Chełmicka","doi":"10.1007/BF01969735","DOIUrl":"https://doi.org/10.1007/BF01969735","url":null,"abstract":"<p><p>Energy expenditure was investigated in 15 patients with liver cirrhosis and 20 healthy controls by three methods: indirect calorimetry, anthropometry using the Harris-Benedict equation and bioelectrical impedance analysis. The energy expenditure was expressed in kcal/day, kcal/kg BW/day (BW - body weight), kcal/kg LBM/day (LBM - lean body mass, derived by bioelectrical impedance analysis) or in kcal/m2/day. We did not find statistical differences between values of resting energy expenditure obtained in patients with cirrhosis of the liver and healthy controls whichever method we used. We also did not find statistical differences between values obtained by indirect calorimetry, anthropometry and bioelectrical impedance analysis. There was a significant correlation between indirect calorimetry and anthropometry in both groups. We found significant correlations between indirect calorimetry and anthropometry, and between indirect calorimetry and bioelectrical impedance analysis, in the control group only. We can conclude that (1) resting energy expenditure of patients with cirrhosis of the liver is not changed when compared with healthy controls, and (2) bioelectrical impedance is a useful method to calculate body composition from which energy expenditure is derived; however, it gives an appropriate result only in healthy people, and only approximate values in patients with cirrhosis.</p>","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 6","pages":"591-6"},"PeriodicalIF":0.0,"publicationDate":"1996-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01969735","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19671344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Antioxidant survey to assess antagonism to redox stress using a prokaryotic and an eukaryotic system. 利用原核和真核系统对氧化还原应激的拮抗作用进行研究。
Experientia Pub Date : 1996-06-15 DOI: 10.1007/BF01969736
H Baker, B DeAngelis, O Frank, M Khalil, S H Hutner, E R Baker
{"title":"Antioxidant survey to assess antagonism to redox stress using a prokaryotic and an eukaryotic system.","authors":"H Baker,&nbsp;B DeAngelis,&nbsp;O Frank,&nbsp;M Khalil,&nbsp;S H Hutner,&nbsp;E R Baker","doi":"10.1007/BF01969736","DOIUrl":"https://doi.org/10.1007/BF01969736","url":null,"abstract":"<p><p>Using a prokaryote (Escherichia coli) and a metazoa-resembling eukaryote (Ochromonas danica), we surveyed antioxidants which might overcome redox stress imposed by menadione sodium bisulphite (MD) and buthionine sulphoximine (BSO). BSO oxidant stress was evident only in O. danica; MD oxidant stress was evident in both organisms. Glutathione, its precursors, e.g. cysteine, homocysteine, and 2-oxo-4-thiazolidine carboxylic acid, and red blood cells, emerged as prime antioxidants for relieving BSO and MD oxidant stress. BSO and MD oxidant activity and antioxidant-annulling effect in O. danica were judged comparable to those found in animal cells whereas the results E. coli were not entirely equivalent. The O. danica system emerged as a practical, rapid, and useful system for pinpointing oxidant stressors and antioxidants, and shows promise for studies with mammalian systems.</p>","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 6","pages":"597-9"},"PeriodicalIF":0.0,"publicationDate":"1996-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01969736","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19671345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Antibacterial effect of 2-hydroxy-N-(3,4-dimethyl-5-isoxazolyl)-1, 4-naphthoquinone-4-imine on Staphylococcus aureus. 2-羟基-n -(3,4-二甲基-5-异恶唑基)- 1,4 -萘醌-4-亚胺对金黄色葡萄球菌的抑菌作用。
Experientia Pub Date : 1996-06-15 DOI: 10.1007/BF01969737
P Bogdanov, I Albesa, N R Sperandeo, C Luna, M M de Bertorello
{"title":"Antibacterial effect of 2-hydroxy-N-(3,4-dimethyl-5-isoxazolyl)-1, 4-naphthoquinone-4-imine on Staphylococcus aureus.","authors":"P Bogdanov,&nbsp;I Albesa,&nbsp;N R Sperandeo,&nbsp;C Luna,&nbsp;M M de Bertorello","doi":"10.1007/BF01969737","DOIUrl":"https://doi.org/10.1007/BF01969737","url":null,"abstract":"<p><p>The mechanism by which a new naphthoquinone derivative, the 2-hydroxy-N-(3,4-dimethyl-5-isoxazolyl)-1, 4-naphthoquinone-4-imine (INQI-E) has antibacterial effect against Staphylococcus aureus was studied. The interaction of INQI-E with the bacteria was followed by absorption spectroscopy at 323 and 490 nm. The absorption band of INQI-E at 490 nm undergoes a hypochromic shift with a decrease of intensity. This effect was found to be reversible by oxygenation during the first hours of incubation. The participation of an oxidation-reduction process related to the respiratory chain was demonstrated by oxygen consumption. An increase in O2 uptake and inhibition of S. aureus growth was observed. Experiments with three inhibitors of the respiratory chain demonstrated that the pathway induced by INQI-E was antimycin-resistant and KCN- and salicylhydroxamic acid (SHAM)-sensitive, which suggests that INQI-E is capable of diverting the normal electron flow to an alternate superoxide-producing route. On the other hand, experiments with Tiron, a specific scavenger of superoxide, hindered the effect of INQI-E against S. aureus, indicating that the inhibitory growth effect of this quinone-imine is mainly due to the production of the cytotoxic superoxide radical.</p>","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 6","pages":"600-4"},"PeriodicalIF":0.0,"publicationDate":"1996-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01969737","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19671346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Inhibition of invasion and intraerythrocytic development of Plasmodium falciparum by kinase inhibitors. 激酶抑制剂对恶性疟原虫侵袭及红细胞发育的抑制作用。
Experientia Pub Date : 1996-06-15 DOI: 10.1007/BF01969742
A R Dluzewski, C R Garcia
{"title":"Inhibition of invasion and intraerythrocytic development of Plasmodium falciparum by kinase inhibitors.","authors":"A R Dluzewski,&nbsp;C R Garcia","doi":"10.1007/BF01969742","DOIUrl":"https://doi.org/10.1007/BF01969742","url":null,"abstract":"<p><p>We have examined the effects of seven protein kinase inhibitors (staurosporine, genistein, methyl 2,5-dihydroxycinnamate, tyrphostins B44 and B46, lavendustin A and R03) on the erythrocytic cycle of the malaria parasite, Plasmodium falciparum. One (staurosporine) strongly inhibits serine/threonine kinases, but the remainder all exhibit a strong preference for tyrosine kinases. We have been able to discriminate between effects on invasion and on intraerythrocytic development. All reagents impeded development of intraerythrocytic parasites, though at widely differing concentrations, from the sub-micromolar to the millimolar. Several inhibitors, including staurosporine, also reduced invasion. The phosphatase inhibitor, okadaic acid, had a strong inhibitory effect both on invasion and development. The regulation of malaria development by phosphorylation or dephosphorylation reactions at several points in the blood-stage cycle is implied.</p>","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 6","pages":"621-3"},"PeriodicalIF":0.0,"publicationDate":"1996-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01969742","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19671350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 47
In situ hybridization analysis of globin mRNAs in the primitive erythroid cells of the chick embryo. 鸡胚原始红细胞珠蛋白mrna的原位杂交分析。
Experientia Pub Date : 1996-06-15 DOI: 10.1007/BF01969723
L Fucci, U Galderisi, M Piscopo, R del Gaudio, G Geraci
{"title":"In situ hybridization analysis of globin mRNAs in the primitive erythroid cells of the chick embryo.","authors":"L Fucci,&nbsp;U Galderisi,&nbsp;M Piscopo,&nbsp;R del Gaudio,&nbsp;G Geraci","doi":"10.1007/BF01969723","DOIUrl":"https://doi.org/10.1007/BF01969723","url":null,"abstract":"<p><p>The possibility that the minor embryonic chick hemoglobins might be present in a particular subgroup of primitive erythroid cells has been investigated by in situ hybridization. Probe to detect the mRNA for the alpha A globin chain of the minor embryonic hemoglobin was used, and the results of the hybridization were compared with those obtained using as probes the cDNAs for total globin mRNAs. All erythroid cells circulating in a 4-day-old chick embryo gave positive signals with both probes at an approximately constant ratio. This shows that all cells contain a similar assortment of hemoglobin types, excluding the possibility that a subgroup might contain the minor primitive hemoglobins exclusively. However, the cells are not homogeneous, since about 10% of them show a distinctly higher concentration of mRNA of all globin types.</p>","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 6","pages":"535-9"},"PeriodicalIF":0.0,"publicationDate":"1996-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01969723","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19672220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increased melatonin levels after hemorrhagic shock in male and female C3H/HeN mice. 雄性和雌性C3H/HeN小鼠失血性休克后褪黑激素水平升高。
Experientia Pub Date : 1996-06-15 DOI: 10.1007/BF01969734
M W Wichmann, R Zellweger, C M DeMaso, A Ayala, I H Chaudry
{"title":"Increased melatonin levels after hemorrhagic shock in male and female C3H/HeN mice.","authors":"M W Wichmann,&nbsp;R Zellweger,&nbsp;C M DeMaso,&nbsp;A Ayala,&nbsp;I H Chaudry","doi":"10.1007/BF01969734","DOIUrl":"https://doi.org/10.1007/BF01969734","url":null,"abstract":"<p><p>Although hemorrhagic shock leads to significant alterations of several hormones, e.g. ACTH, corticosterone and beta-endorphin, it is not known whether plasma melatonin levels are affected under this condition and if so, whether the effects are comparable in males and females. Using a radioimmunoassay, it was found that plasma melatonin levels were significantly increased in male and proestrus female C3H/HeN mice immediately after hemorrhagic shock. However, in male mice, by two hours after hemorrhage and resuscitation, plasma melatonin returned to levels comparable to those seen in control and sham-operated animals. Proestrus female mice, on the other hand, showed significantly increased plasma melatonin levels at two hours after surgery when compared to unoperated control animals. Although the significance and biological role of the transient increased plasma melatonin levels after hemorrhagic shock remain to be determined, it appears that the pineal gland and/or an extrapineal source of melatonin, of both male and proestrus female mice responds to severe hypotension by increased release of melatonin.</p>","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 6","pages":"587-90"},"PeriodicalIF":0.0,"publicationDate":"1996-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01969734","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19671343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Tocopheryl acetates from the pupal exocrine secretion of the squash beetle, Epilachna borealis (Coccinellidae). 从北瓢虫科的南瓜甲虫蛹分泌的外分泌中提取生育酚醋酸酯。
Experientia Pub Date : 1996-06-15 DOI: 10.1007/BF01969741
A B Attygalle, S R Smedley, T Eisner, J Meinwald
{"title":"Tocopheryl acetates from the pupal exocrine secretion of the squash beetle, Epilachna borealis (Coccinellidae).","authors":"A B Attygalle,&nbsp;S R Smedley,&nbsp;T Eisner,&nbsp;J Meinwald","doi":"10.1007/BF01969741","DOIUrl":"https://doi.org/10.1007/BF01969741","url":null,"abstract":"<p><p>The oily droplets on the pupal integumental hairs of the squash beetle Epilachna borealis contain a mixture of alpha-, beta-, gamma-, and delta-tocopheryl acetates as major constituents. In addition, the secretion contains a number of minor components that appear to be dehydrocongeners of the major components. This is the first report of the occurrence of acetate esters of any tocopherol in nature.</p>","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 6","pages":"616-20"},"PeriodicalIF":0.0,"publicationDate":"1996-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01969741","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19671349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
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