Experientia最新文献_第6页

Tissue protection against oxidative stress. 组织对氧化应激的保护。

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923990

S Di Meo, P Venditti, T De Leo

{"title":"Tissue protection against oxidative stress.","authors":"S Di Meo, P Venditti, T De Leo","doi":"10.1007/BF01923990","DOIUrl":"https://doi.org/10.1007/BF01923990","url":null,"abstract":"We used an enhanced luminescence technique to study the response of rat tissues, such as liver, heart, muscle and blood, to oxidative stress and to determine their antioxidant capacity. As previously found for liver homogenate, the intensity of light emission (E) of tissue homogenates and blood samples, stressed with sodium perborate, is dependent on concentration, and the dose-response curves can be described by the equation E = a.C/exp(b.C). The b value depends on the antioxidant defence capability of the tissues. In fact, it increases when homogenates are supplemented with an antioxidant, and is correlated with tissue antioxidant capacity, evaluated by two previously set up methods both using the same luminescence technique. Our results indicate that the order of antioxidant capacity of the tissues is liver > blood > heart > muscle. The a value depends on the systems catalysing the production of radical species. In fact, it is related to the tissue level of hemoproteins, which are known to act as catalysts in radical production from hydroperoxides. The equation proposed to describe the dose-response relation is simple to handle and permits an immediate connection with the two characteristics of the systems analysed which determine their response to the pro-oxidant treatment. However, the equation which best describes the above relation for all the tissues is the following: E = alpha. C/exp(beta.C delta). The parameter delta assumes values smaller than 1, which seem to depend on relative amounts of tissue hemoproteins and antioxidants. The extension of the analysis to mitochondria shows that they respond to oxidative stress in a way analogous to the tissues, and that the adherence of the dose-response curve to the course predicted from the equation E = a.C/exp(b.C) is again dependent on hemoprotein content.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 8","pages":"786-94"},"PeriodicalIF":0.0,"publicationDate":"1996-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01923990","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19746762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Characterization and relative abundance of maxi-chloride channels in Epstein-Barr virus (EBV) producer: B95-8 cells. eb病毒(EBV)产生者:B95-8细胞中最大氯离子通道的特征和相对丰度。

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923996

T H Yeh, M C Tsai, S Y Lee, M M Hsu

{"title":"Characterization and relative abundance of maxi-chloride channels in Epstein-Barr virus (EBV) producer: B95-8 cells.","authors":"T H Yeh, M C Tsai, S Y Lee, M M Hsu","doi":"10.1007/BF01923996","DOIUrl":"https://doi.org/10.1007/BF01923996","url":null,"abstract":"Several Epstein-Barr virus (EBV)-transformed cell lines were used to investigate the pathogenesis of lymphoproliferative diseases and nasopharyngeal carcinoma. The studies focus on the events occurring inside the membrane. On only one occasion, the cell membrane of EBV-transformed B lymphocytes from a cystic fibrosis patient was found to express defective Cl channels (CFTR; Cystic Fibrosis Transmembrane conductance Regulator), as in the airway epithelial cell. No other type of channel in EBV-transformed cells has so far been investigated. In this study, the cell membrane of the B95-8 cell was examined by the patch-clamp technique and compared to the non-EBV-infected BJAB cell. The high conductance (approximately 300 pS) maxi-chloride (Cl) channel activity was the most frequently observed event in inside-out configurations. Under similar experimental conditions, we have found a significantly higher probability of detecting maxi-Cl channel activity on the cell membrane of B95-8 cells (69%) than on BJAB cells (27%), or as previously reported on resting murine B lymphocytes (38%) or intact human T lymphocytes (37%). The relative abundance of the maxi-Cl channel on B95-8 cells may be linked to EBV infection and/or secretory ability.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 8","pages":"818-26"},"PeriodicalIF":0.0,"publicationDate":"1996-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01923996","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19746652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effects of female sex hormones on polyamine-oxidizing enzyme activities and polyamine concentrations in immature rat uterus and liver. 雌性荷尔蒙对未成熟大鼠子宫和肝脏中多胺氧化酶活性和多胺浓度的影响

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923991

O Dimitrov, V Pavlov, I Jotova

{"title":"Effects of female sex hormones on polyamine-oxidizing enzyme activities and polyamine concentrations in immature rat uterus and liver.","authors":"O Dimitrov, V Pavlov, I Jotova","doi":"10.1007/BF01923991","DOIUrl":"10.1007/BF01923991","url":null,"abstract":"17 beta-estradiol (E2) and progesterone (P) treatment of immature female rats (10 micrograms/100 g body weight) respectively resulted in 1.38-fold (p < 0.02) and 1.42-fold (p < 0.02) increase in the uterine polyamine oxidase activity, and 2.45-fold (p < 0.001) and 1.43-fold (p < 0.02) increase in the uterine diamine oxidase activity, as compared to the controls. E2 caused a 5-fold (p < 0.05) and a 1.36-fold (p < 0.05) increase in putrescine and spermidine concentration respectively in rat uterus. Increases of 1.7-fold (p < 0.02) and 1.6-fold (p < 0.05) in putrescine and spermine concentration were determined in the P-treated uterus, as compared to the controls. The spermidine/spermine ratio, which is regarded as an index of growth rate, was higher in the E2-treated uterus and lower in the P-treated uterus than in the control uterus. No statistically significant hormonal effects were estimated in the immature liver. The data reported suggest the possibility of an involvement of polyamine-oxidizing enzymes in the modulation of polyamine concentrations in rat uterus by the female sex hormones.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 8","pages":"795-8"},"PeriodicalIF":0.0,"publicationDate":"1996-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01923991","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19746763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Immunochemical characterization of antibody-coated nanoparticles. 抗体包被纳米颗粒的免疫化学表征。

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923993

F Velge-Roussel, P Breton, X Guillon, F Lescure, N Bru, D Bout, J Hoebeke

引用次数: 11

Molecular evidence for increased hematopoietic proliferation in the spleen of the b/b laboratory rat. b/b实验大鼠脾脏造血增殖增加的分子证据。

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923994

S Savković, S Pavlović, T Mitrović, M Joksimović, J Marjanović, V Glisin, Z Popović

引用次数: 1

Embryonic chick muscle produces an FGF-like activity. 鸡胚胎肌肉产生类似fgf的活性。

Experientia Pub Date : 1996-08-15 DOI: 10.1007/BF01923985

D S Morris, S J Stock, J C McLachlan

{"title":"Embryonic chick muscle produces an FGF-like activity.","authors":"D S Morris, S J Stock, J C McLachlan","doi":"10.1007/BF01923985","DOIUrl":"https://doi.org/10.1007/BF01923985","url":null,"abstract":"Normal and pathological formation of blood vessels is of considerable interest both in terms of basic scientific processes and clinical applications. Angiogenic events in the adult are likely to represent persistence of developmental mechanisms, and embryos are therefore a suitable experimental model for these processes. Among embryonic tissues, muscle is particularly appropriate for investigation, since it is highly vascularised from early stages. There are a number of competing explanations of how this process is controlled. Bioassays offer advantages over conventional molecular localisation techniques, in that they reveal the presence of active processed forms of the molecules under study, rather than non-processed forms, or non-translated messages. Using these techniques, we report here that embryonic chick muscle, taken from the stages at which blood vessels are forming, produces an angiogenic activity on the chick chorioallantoic membrane (CAM), and transforms NR6 cells in soft agar. Basic fibroblast growth factor (bFGF) is shown to be angiogenic on the CAM in the same way, and also transforms NR6 cells (NR6 cells lack functional epidermal growth factor/transforming growth factor-a receptors, and are believed to respond only to bFGF in this way). Anti-bFGF removes the transforming activity of the embryonic muscle. We conclude that this represents evidence that embryonic chick muscle is producing an FGF-like molecule which is capable of acting as an angiogenic agent at the appropriate times in development.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 8","pages":"757-61"},"PeriodicalIF":0.0,"publicationDate":"1996-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01923985","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19747504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum cholesterol level in a typical suburban commercial community in Nigeria. 尼日利亚一个典型郊区商业社区的血清胆固醇水平。

Experientia Pub Date : 1996-07-15 DOI: 10.1007/BF01925573

J E Ahaneku, J C Ndefo, C E Dioka

引用次数: 3

Experimental liver fibrosis induced in rats receiving high doses of alcohol and alternating between regular and vitamin-depleted diets. 实验性肝纤维化诱导大鼠接受高剂量的酒精，并在常规和缺乏维生素的饮食之间交替。

Experientia Pub Date : 1996-07-15 DOI: 10.1007/BF01925580

H Hirano, T Hirano, K Hirata, M Tamura, T Yamaura, T Hamada

引用次数: 4

The diet and gut microflora influence the distribution of enteroendocrine cells in the rat intestine. 饲粮和肠道菌群对大鼠肠道内分泌细胞的分布有影响。

Experientia Pub Date : 1996-07-15 DOI: 10.1007/BF01925570

R Sharma, U Schumacher

{"title":"The diet and gut microflora influence the distribution of enteroendocrine cells in the rat intestine.","authors":"R Sharma, U Schumacher","doi":"10.1007/BF01925570","DOIUrl":"https://doi.org/10.1007/BF01925570","url":null,"abstract":"Several functions of the gut are locally influenced by peptides and biogenic amines released from enteroendocrine cells. The aim of the present study was to assess whether the luminal stimulus of diet or microbial flora or diet-microbial interactions have an influence on the distribution of enteroendocrine cells along the crypt-surface axes of the small and large intestine. The effects of diet and indigenous flora were investigated by comparing the numbers of argyrophil and serotonin immunoreactive cells in the jejunum and colon of germ free and conventional rats fed either a purified diet containing fine ingredients or a commercial diet containing crude fibre of cereal origin. The effect of human flora were analysed in germ-free rats inoculated with human faecal organisms. 1. Feeding the commercial diet reduced the number of argyrophil endocrine cells in the jejunum and serotonin immunoreactive cells in the colon of germ-free animals but increased the serotonin immunoreactive cells in the colon of conventional animals. 2. The rat flora increased the serotonin immunoreactive cells in the colon of animals fed a commercial diet and decreased in those fed a purified diet. 3. Inoculation of human flora increased the numbers of serotonin immunoreactive cells both in the jejunum and colon. The results provide evidence that the dietary changes and diet-microbial interactions can affect the regional number of enteroendocrine cells.","PeriodicalId":12087,"journal":{"name":"Experientia","volume":"52 7","pages":"664-70"},"PeriodicalIF":0.0,"publicationDate":"1996-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF01925570","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19671960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Mechanism of age-dependent involution in embryonic chick notochords. 鸡胚脊索年龄依赖性复归的机制。

Experientia Pub Date : 1996-07-15 DOI: 10.1007/BF01925574

E Ghanem, M Cornelissen, H Thierens, L De Ridder

引用次数: 0