European journal of protistology最新文献_第6页

Testate amoebae (Arcellinida, Amoebozoa) community diversity in New England bogs and fens assessed through lineage-specific amplicon sequencing 通过品系特异性扩增子测序评估新英格兰沼泽和沼泽中睾丸变形虫（Arcellinida，变形虫）群落多样性

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-12-15 DOI: 10.1016/j.ejop.2023.126049

Olivia K. Dufour , Evie Hoffman , Robin S. Sleith , Laura A. Katz , Agnes K.M. Weiner , Taylor R. Sehein

{"title":"Testate amoebae (Arcellinida, Amoebozoa) community diversity in New England bogs and fens assessed through lineage-specific amplicon sequencing","authors":"Olivia K. Dufour , Evie Hoffman , Robin S. Sleith , Laura A. Katz , Agnes K.M. Weiner , Taylor R. Sehein","doi":"10.1016/j.ejop.2023.126049","DOIUrl":"10.1016/j.ejop.2023.126049","url":null,"abstract":"<div>Testate amoebae (order Arcellinida) are abundant in freshwater ecosystems, including low pH bogs and fens. Within these environments, Arcellinida are considered top predators in microbial food webs and their tests are useful bioindicators of paleoclimatic changes and anthropogenic pollutants. Accurate species identifications and characterizations of diversity are important for studies of paleoclimate, microbial ecology, and environmental change; however, morphological species definitions mask cryptic diversity, which is a common phenomenon among microbial eukaryotes. Lineage-specific primers recently designed to target Arcellinida for amplicon sequencing successfully captured a poorly-described yet diverse fraction of the microbial eukaryotic community. Here, we leveraged the application of these newly-designed primers to survey the diversity of Arcellinida in four low-pH New England bogs and fens, investigating variation among bogs (2018) and then across seasons and habitats within two bogs (2019). Three OTUs represented 66% of Arcellinida reads obtained across all habitats surveyed. 103 additional OTUs were present in lower abundance with some OTUs detected in only one sampling location, suggesting habitat specificity. By establishing a baseline for Arcellinida diversity, we provide a foundation to monitor key taxa in habitats that are predicted to change with increasing anthropogenic pressure and rapid climate change.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"92 ","pages":"Article 126049"},"PeriodicalIF":2.9,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138689985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of Holostichides (Extraholostichides) eastensis tianjinensis subgen. nov. subspec. nov. (Ciliophora, Hypotricha) from northern China 描述来自中国北方的 Holostichides (Extraholostichides) eastensis tianjinensis subgen.

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-12-12 DOI: 10.1016/j.ejop.2023.126048

Ziyan Liu, Ziyu Wang, Qi Zhang, Qiukun Wang, Fengchao Li

{"title":"Description of Holostichides (Extraholostichides) eastensis tianjinensis subgen. nov. subspec. nov. (Ciliophora, Hypotricha) from northern China","authors":"Ziyan Liu, Ziyu Wang, Qi Zhang, Qiukun Wang, Fengchao Li","doi":"10.1016/j.ejop.2023.126048","DOIUrl":"10.1016/j.ejop.2023.126048","url":null,"abstract":"<div>The morphology and morphogenesis of a new urostylid, Holostichides (Extraholostichides) eastensis tianjinensis subgen. nov. subspec. nov. were analyzed. The new subspecies differs from the nominotypical subspecies H. (Extraholostichides) eastensis eastensis Wang et al., 2022 by the relatively long frontoterminal row (about 60% vs. 30% of body length), colorless cortical granules (vs. dark brown), two (vs. one) parabuccal cirri, and usually an extra cirrus behind the first midventral pair (vs. lacking). Based on the difference in the frontal ciliature, we split Holostichides into two subgenera: H. (Extraholostichides) subgen. nov. (type species Holostichides eastensis Wang et al., 2022; with a short cirral row behind the middle frontal cirrus) and H. (Holostichides) Foissner, 1987 (type species Holostichides chardezi Foissner, 1987; lacking this short row). The main morphogenetic characters of the new subspecies are very similar to those of H. (Extraholostichides) eastensis eastensis except for some minor differences. Phylogenetic analyses based on SSU rDNA sequences indicate that H. (Extraholostichides) subgen. nov. is monophyletic and nested within the monophyletic genus Holostichides, which is sister to Eschaneustyla lugeri.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"92 ","pages":"Article 126048"},"PeriodicalIF":2.9,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138572377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and molecular phylogeny of two hypotrichous ciliates (Ciliophora, Spirotrichea) from South Korea, including Hemiurosomoida koreana n. sp. 韩国两种次毛纤毛虫(纤毛虫、螺旋体纤毛虫)的形态和分子系统发育，包括朝鲜半毛纤毛虫

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-12-06 DOI: 10.1016/j.ejop.2023.126045

Atef Omar , Ji Hye Moon , Jae-Ho Jung

{"title":"Morphology and molecular phylogeny of two hypotrichous ciliates (Ciliophora, Spirotrichea) from South Korea, including Hemiurosomoida koreana n. sp.","authors":"Atef Omar , Ji Hye Moon , Jae-Ho Jung","doi":"10.1016/j.ejop.2023.126045","DOIUrl":"10.1016/j.ejop.2023.126045","url":null,"abstract":"<div>The living morphology, infraciliature, and molecular phylogeny of a new soil ciliate, Hemiurosomoida koreana n. sp., discovered in a sample collected from a mountain in the northeast of South Korea, were investigated. The new species possesses the characteristics of the genus Hemiurosomoida, i.e., a reduced number of frontal-ventral-transverse cirri, three dorsal kineties of which kineties 1 and 2 each bears a caudal cirrus, and a single dorsomarginal kinety. It is distinguishable from congeners and other similar species by at least one distinct qualitative or quantitative character including the body size, the presence and arrangement of cortical granules, the number of adoral membranelles, marginal cirri, and dorsal dikinetids, or by the arrangement of transverse cirri. Phylogenetic analyses based on 18S rRNA gene sequences also support the assignment of the new species to the non-monophyletic genus Hemiurosomoida. In addition, the living morphology, infraciliature, and the 18S rRNA gene sequence of a Korean population of Nothoholosticha flava were studied.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"92 ","pages":"Article 126045"},"PeriodicalIF":2.9,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138534298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology, morphogenesis, and molecular phylogeny of Aspidisca koreana n. sp. (Ciliophora, Euplotida) from South Korea 韩国朝鲜蛛的形态、形态发生和分子系统发育(纤毛蛛科，整虫目)

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-12-01 DOI: 10.1016/j.ejop.2023.126037

Ji Hye Choi , Atef Omar , Jae-Ho Jung

引用次数: 0

Morphology and molecular phylogeny of two new species of Psammophaga (Rhizaria, Foraminifera) from the west coast of India 印度西海岸两新种的形态和分子系统发育(根状目，有孔虫目)

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-11-21 DOI: 10.1016/j.ejop.2023.126035

Tushar Kaushik , Vaishnavi Dixit , Thirumalai Murugan

{"title":"Morphology and molecular phylogeny of two new species of Psammophaga (Rhizaria, Foraminifera) from the west coast of India","authors":"Tushar Kaushik , Vaishnavi Dixit , Thirumalai Murugan","doi":"10.1016/j.ejop.2023.126035","DOIUrl":"10.1016/j.ejop.2023.126035","url":null,"abstract":"<div>Benthic foraminifera are unicellular eukaryotic protists that construct an organic, agglutinated, or calcareous test wall. Although single-chambered (monothalamous) foraminifera are ubiquitous in marine habitats worldwide, they are poorly known compared to their multi-chambered relatives, notably from the tropical marine environments of India. In this study, we describe two new species of marine monothalamid genus Psammophaga Arnold, 1982, from the Rajapuri Creek, coastal Maharashtra, India (Arabian Sea). Psammophaga holzmannae sp. nov. is ovoid to spherical shaped, 103–246 µm in length, single aperture, translucent to orange color cytoplasm, outer surface is composed of agglutinated fine clay particles, and ingested mineral grains are concentrated near its aperture. Psammophaga sinhai sp. nov. is oblong, elliptical, or droplet-shaped, 279–448 µm in length, single aperture, yellow olive color cytoplasm, the exterior surface formed of agglutinated fine clay particles, and the ingested mineral grains are dispersed throughout the body. Phylogenetic analyses based on partial small subunit rRNA gene sequences position new species within the Clade E of monothalamids and are genetically distinct from other Psammophaga. Elemental (SEM–EDS) analysis of engulfed mineral grains revealed preferential selection and uptake of heavy opaque titaniferous minerals from the ambient environment in the cytoplasm.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"92 ","pages":"Article 126035"},"PeriodicalIF":2.9,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138543329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification and in-silico analysis of papain-family cysteine protease encoding genes in Tetrahymena thermophila 嗜热四膜虫木瓜蛋白酶家族半胱氨酸蛋白酶编码基因的全基因组鉴定和内部分析

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-11-08 DOI: 10.1016/j.ejop.2023.126033

Şeyma DURAN , Ayça Fulya ÜSTÜNTANIR DEDE , Yeliz DÜNDAR ORHAN , Muhittin ARSLANYOLU

{"title":"Genome-wide identification and in-silico analysis of papain-family cysteine protease encoding genes in Tetrahymena thermophila","authors":"Şeyma DURAN , Ayça Fulya ÜSTÜNTANIR DEDE , Yeliz DÜNDAR ORHAN , Muhittin ARSLANYOLU","doi":"10.1016/j.ejop.2023.126033","DOIUrl":"10.1016/j.ejop.2023.126033","url":null,"abstract":"<div>Tetrahymena thermophila is a promising host for recombinant protein production, but its utilization in biotechnology is mostly limited due to the presence of intracellular and extracellular papain-family cysteine proteases (PFCPs). In this study, we employed bioinformatics approaches to investigate the T. thermophila PFCP genes and their encoded proteases (TtPFCPs), the most prominent protease family in the genome. Results from the multiple sequence alignment, protein modeling, and conserved motif analyses revealed that all TtPFCPs showed considerably high homology with mammalian cysteine cathepsins and contained conserved amino acid motifs. The total of 121 TtPFCP-encoding genes, 14 of which were classified as non-peptidase homologs, were found. Remaining 107 true TtPFCPs were divided into four distinct subgroups depending on their homology with mammalian lysosomal cathepsins: cathepsin L-like (TtCATLs), cathepsin B-like (TtCATBs), cathepsin C-like (TtCATCs), and cathepsin X-like (TtCATXs) PFCPs. The majority of true TtPFCPs (96 out of the total) were in TtCATL-like peptidase subgroup. Both phylogenetic and chromosomal localization analyses of TtPFCPs supported the hypothesis that TtPFCPs likely evolved through tandem gene duplication events and predominantly accumulated on micronuclear chromosome 5. Additionally, more than half of the identified TtPFCP genes are expressed in considerably low quantities compared to the rest of the TtPFCP genes, which are expressed at a higher level. However, their expression patterns fluctuate based on the stage of the life cycle. In conclusion, this study provides the first comprehensive in-silico analysis of TtPFCP genes and encoded proteases. The results would help designing an effective strategy for protease knockout mutant cell lines to discover biological function and to improve the recombinant protein production in T. thermophila.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"92 ","pages":"Article 126033"},"PeriodicalIF":2.9,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135516519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two new species of the genus Leptomyxa (Goodey, 1915) – Leptomyxa botanica n. sp. and Leptomyxa monrepos n. sp. from the soil habitats of Northwestern Russia Leptomyxa属二新种(Goodey, 1915)——来自俄罗斯西北部土壤生境的Leptomyxa botanica n. sp.和Leptomyxa monrepos n. sp.。

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-10-01 DOI: 10.1016/j.ejop.2023.126028

Nikita S. Kulishkin , Alexey V. Smirnov , David J. Patterson

{"title":"Two new species of the genus Leptomyxa (Goodey, 1915) – Leptomyxa botanica n. sp. and Leptomyxa monrepos n. sp. from the soil habitats of Northwestern Russia","authors":"Nikita S. Kulishkin , Alexey V. Smirnov , David J. Patterson","doi":"10.1016/j.ejop.2023.126028","DOIUrl":"10.1016/j.ejop.2023.126028","url":null,"abstract":"<div>Amoebae of the genus Leptomyxa have variable morphologies and can only be reliably identified using molecular data. However, species distinction based on the 18S rRNA gene sequence is difficult due to the very low level of sequence divergence among morphologically different species. The database for other genes is much smaller, and genomic data on Leptomyxa is almost absent. In this study, we describe two new terrestrial species of the genus Leptomyxa isolated from Northwestern Russia, Leptomyxa botanica n. sp. and Leptomyxa monrepos n. sp. Both species easily adopt an expanded fan-shaped form and have a complex structure of the nucleolar material. Phylogenetic analyses show a derived status of these two species. They form a clade with Leptomyxa valladaresi. Our tree confirms that the 18S rRNA gene sequences of Leptomyxa species are split into two large clades. The morphological synapomorphies of these clades are not obvious. This analysis is complicated by the lack of reliable morphological data on many sequenced strains and probable misidentification of some isolates.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"91 ","pages":"Article 126028"},"PeriodicalIF":2.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89717416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic position of the pelobiont Mastigamoeba aspera and revision of the family Mastigamoebidae (Archamoebae, Pelobiontida) Mastigamoeba aspera pelobiont的系统发育位置和Mastigamoebidae科（Archamoebae，Pelobiotida）的修订。

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-10-01 DOI: 10.1016/j.ejop.2023.126029

Ludmila V. Chistyakova , Alexei Yu. Kostygov , Andrew V. Goodkov , Anton V. Radaev , Alexander O. Frolov

引用次数: 0

Re-isolation and revaluation of boundaries of Thecamoeba terricola Greeff, 1886 (Amoebozoa, Discosea, Thecamoebida), with description of Thecamoeba vicaria n. sp. 重新隔离和重新评估terricola Greeff的边界，1886年（Amoebozoa，Discosea，Thecamoebida），描述了Thecamoeba vicaria n.sp。

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-10-01 DOI: 10.1016/j.ejop.2023.126030

Yelisei Mesentsev , Alina Surkova , Oksana Kamyshatskaya , Elena Nassonova , Alexey Smirnov

{"title":"Re-isolation and revaluation of boundaries of Thecamoeba terricola Greeff, 1886 (Amoebozoa, Discosea, Thecamoebida), with description of Thecamoeba vicaria n. sp.","authors":"Yelisei Mesentsev , Alina Surkova , Oksana Kamyshatskaya , Elena Nassonova , Alexey Smirnov","doi":"10.1016/j.ejop.2023.126030","DOIUrl":"10.1016/j.ejop.2023.126030","url":null,"abstract":"<div>We studied a large species of Thecamoeba found in a glass dish with soaked moss and obtained 18s rRNA gene sequence of this organism. Morphologically, the strain was most similar to T. terricola sensu Page, but had significant differences in cell size and nuclear morphology. A more complete similarity was found with the original description of “Amoeba terricola” by Greeff, as well as with Penard’s descriptions and slides. The analysis of literature data shows that the strain described by Page in 1977 as a re-isolated T. terricola differs from the original description of this species provided by Greeff in 1866 and data by Penard published in 1902 and 1913. Based on our observations as well as on Greeff’s and Penard’s data, we reassessed the species boundaries of T. terricola and established T. vicaria n. sp. for the organism described by Page in 1977. The species T. terricola was defined according to its original description. The observations of amoebae on agar have shown that T. terricola cells can form the “standing amoeba” stage, previously described only for Sappinia pedata. This and some other “behaviour” features of T. terricola may be associated with living conditions in terrestrial habitats.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"91 ","pages":"Article 126030"},"PeriodicalIF":2.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71479727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological, ontogenetic, and phylogenetic research of a poorly known non-dorsomarginalian ciliate: Erniella filiformis Foissner, 1987 (Protista, Ciliophora, Hypotrichia) 一种鲜为人知的非背侧精缘纤毛虫的形态学、个体发生学和系统发育研究：丝状Erniella filiformis Foissner，1987（Protista，Ciliophora，Hyptrichia）。

IF 2.9 2区生物学

European journal of protistology Pub Date : 2023-10-01 DOI: 10.1016/j.ejop.2023.126031

Yurui Wang, Jingyi Wang, Banghe Wang, Kunwu Wang, Chen Shao

{"title":"Morphological, ontogenetic, and phylogenetic research of a poorly known non-dorsomarginalian ciliate: Erniella filiformis Foissner, 1987 (Protista, Ciliophora, Hypotrichia)","authors":"Yurui Wang, Jingyi Wang, Banghe Wang, Kunwu Wang, Chen Shao","doi":"10.1016/j.ejop.2023.126031","DOIUrl":"10.1016/j.ejop.2023.126031","url":null,"abstract":"<div>Erniella Foissner, 1987 is a poorly known genus with its available information limited solely to morphological characteristics. In this study, the type species E. filiformis, isolated from highly saline water in China, was investigated for morphology, morphogenesis, and phylogeny based on small subunit ribosomal DNA sequences. The main characteristic events during morphogenesis in Erniella filiformis are as follows: (1) in the proter, only the posterior part of the parental adoral zone is renewed and undulating membranes anlage is formed from the dedifferentiation of the old structure; (2) the oral primordium is formed intrakinetally in the opisthe; (3) frontoventral-transverse cirral anlagen II–IV develop in the primary mode, anlage V develops intrakinetally in the proter; and (4) the right frontoventral row is formed by a single anlage. The phylogenetic analyses indicate that Erniella forms a monophyletic group with Bistichella, Keronopsis, Lamtostyla, Orthoamphisiella, Parabistichella, Paraholosticha, and Uroleptoides.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"91 ","pages":"Article 126031"},"PeriodicalIF":2.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71479726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0