European journal of protistology最新文献_第6页

An approach to evaluating seasonal responses to acute toxicity of antibiotic nitrofurazone on periphytic ciliated protist communities in marine environments 海洋环境中抗生素硝呋酮急性毒性对附生纤毛原虫群落季节性反应的评估方法

IF 2.9 2区生物学

European journal of protistology Pub Date : 2024-04-08 DOI: 10.1016/j.ejop.2024.126081

Awais Ali , Ning Wang , Qiaoling Wang , Guangjian Xu , Henglong Xu

{"title":"An approach to evaluating seasonal responses to acute toxicity of antibiotic nitrofurazone on periphytic ciliated protist communities in marine environments","authors":"Awais Ali , Ning Wang , Qiaoling Wang , Guangjian Xu , Henglong Xu","doi":"10.1016/j.ejop.2024.126081","DOIUrl":"https://doi.org/10.1016/j.ejop.2024.126081","url":null,"abstract":"<div>Periphytic protists including ciliates are the primary components of microbial communities in which they play a vital role in the progression of food webs by moving resources from lower to higher trophic levels. However, the toxic effects of veterinary antibiotics on periphytic protists across four seasons are minimally understood. Therefore, in this study, a 1-year survey was conducted with the antibiotic nitrofurazone (NFZ) applied at concentrations of 0.0, 1.5, 3.0, 6.0, and 12.0 mg/L. Samples of protist communities were collected using microscope glass slides during four seasons in the coastal waters of the Yellow Sea, Qingdao, northern China. The abundance of protists dropped with an increase in NFZ concentrations, and almost all species were dead at a concentration of 12.0 mg/L. The 12 h-LC50 values of NFZ for the protist biota were similar among the four seasons, despite significant seasonal variability in the community structure. The present results suggest that the periphytic protist biota may be used as a biomarker for assessing the ecotoxicity of NFZ in marine environments regardless of the year season.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"94 ","pages":"Article 126081"},"PeriodicalIF":2.9,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140552655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pacific marine gregarines (Apicomplexa) shed light on biogeographic speciation patterns and novel diversity among early apicomplexans 太平洋海洋鳕形目（Apicomplexa）揭示了生物地理学上的物种分化模式和早期无脊椎动物的新多样性

IF 2.9 2区生物学

European journal of protistology Pub Date : 2024-03-30 DOI: 10.1016/j.ejop.2024.126080

Eric Odle , Siratee Riewluang , Kentaro Ageishi , Hiroshi Kajihara , Kevin C. Wakeman

{"title":"Pacific marine gregarines (Apicomplexa) shed light on biogeographic speciation patterns and novel diversity among early apicomplexans","authors":"Eric Odle , Siratee Riewluang , Kentaro Ageishi , Hiroshi Kajihara , Kevin C. Wakeman","doi":"10.1016/j.ejop.2024.126080","DOIUrl":"10.1016/j.ejop.2024.126080","url":null,"abstract":"<div>Gregarines are the most biodiverse group of apicomplexan parasites. This group specializes on invertebrate hosts (e.g., ascidians, crustaceans, and polychaetes). Marine gregarines are of particular interest because they are considered to be the earliest evolving apicomplexan lineage, having subsequently speciated (and radiated) through virtually all existing animal groups. Still, mechanisms governing the broad (global) distribution and speciation patterns of apicomplexans are not well understood. The present study examines Pacific lecudinids, one of the most species-rich and diverse groups of marine gregarines. Here, marine polychaetes were collected from intertidal zones. Single trophozoite cells were isolated for light and electron microscopy, as well as molecular phylogenetic analyses using the partial 18S rRNA gene. The cytochrome c oxidase subunit 1 gene was used to confirm morphology-based host identification. This study introduces Undularius glycerae n. gen., n. sp. and Lecudina kitase n. sp. (Hokkaido, Japan), as well as Difficilina fasoliformis n. sp. (California, USA). Occurrences of Lecudina cf. longissima and Lecudina cf. tuzetae (California, USA) are also reported. Phylogenetic analysis revealed a close relationship between L. pellucida, L. tuzetae, and L. kitase n. sp. Additionally, clustering among North Atlantic and Pacific L. tuzetae formed a species complex, likely influenced by biogeography.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"94 ","pages":"Article 126080"},"PeriodicalIF":2.9,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140404776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological reconstruction during cell regeneration in the ciliate Spirostomum ambiguum 纤毛虫歧螺母细胞再生过程中的形态重建

IF 2.9 2区生物学

European journal of protistology Pub Date : 2024-03-28 DOI: 10.1016/j.ejop.2024.126079

Maho Shimada , Masashi M. Hayakawa , Toshinobu Suzaki , Hideki Ishida

{"title":"Morphological reconstruction during cell regeneration in the ciliate Spirostomum ambiguum","authors":"Maho Shimada , Masashi M. Hayakawa , Toshinobu Suzaki , Hideki Ishida","doi":"10.1016/j.ejop.2024.126079","DOIUrl":"10.1016/j.ejop.2024.126079","url":null,"abstract":"<div>When the ciliate Spirostomum ambiguum is transected into two pieces, both fragments regenerate and proliferate. In the anterior fragments, which have lost their contractile vacuoles due to transection, new contractile vacuoles were formed at their posterior ends in a few minutes. When the cells were cut into three pieces, new contractile vacuoles were formed in the anterior and middle fragments, both at their posterior ends. Thus, the anterior-posterior axis of S. ambiguum was maintained after transection. Morphological repair, including the formation of the contractile vacuole, was also observed when only the anteriormost portion was transected to cut out a small fragment that did not contain part of the macronucleus. Scanning electron microscopy was performed to observe changes in the shape of the cleavage surface of S. ambiguum during the wound healing process. Within minutes after cutting, the cut surface was covered with a cilia-free membrane, preventing leakage of cytoplasmic contents. The surface of the cut area then rounded with time and was covered with cilia, completing the repair of the cut area in about one day.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"94 ","pages":"Article 126079"},"PeriodicalIF":2.9,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140399204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contractile vacuoles: a rapidly expanding (and occasionally diminishing?) understanding 收缩空泡：迅速扩大（偶尔缩小？

IF 2.9 2区生物学

European journal of protistology Pub Date : 2024-03-26 DOI: 10.1016/j.ejop.2024.126078

Kiran J. More , Harpreet Kaur , Alastair G.B. Simpson , Frederick W. Spiegel , Joel B. Dacks

引用次数: 0

Homo- and hetero-oligomeric protein–protein associations explain autocrine and heterologous pheromone-cell interactions in Euplotes 同源和异源异构体蛋白-蛋白关联解释了幼虫的自分泌和异源信息素-细胞相互作用

IF 2.9 2区生物学

European journal of protistology Pub Date : 2024-03-16 DOI: 10.1016/j.ejop.2024.126075

Claudio Alimenti , Bill Pedrini , Pierangelo Luporini , Yaohan Jiang , Adriana Vallesi

{"title":"Homo- and hetero-oligomeric protein–protein associations explain autocrine and heterologous pheromone-cell interactions in Euplotes","authors":"Claudio Alimenti , Bill Pedrini , Pierangelo Luporini , Yaohan Jiang , Adriana Vallesi","doi":"10.1016/j.ejop.2024.126075","DOIUrl":"10.1016/j.ejop.2024.126075","url":null,"abstract":"<div>In Euplotes, protein pheromones regulate cell reproduction and mating by binding cells in autocrine or heterologous fashion, respectively. Pheromone binding sites (receptors) are identified with membrane-bound pheromone isoforms determined by the same genes specifying the soluble forms, establishing a structural equivalence in each cell type between the two twin proteins. Based on this equivalence, autocrine and heterologous pheromone/receptor interactions were investigated analyzing how native molecules of pheromones Er-1 and Er-13, distinctive of mating compatible E. raikovi cell types, associate into crystals. Er-1 and Er-13 crystals are equally formed by molecules that associate cooperatively into oligomeric chains rigorously taking a mutually opposite orientation, and each burying two interfaces. A minor interface is pheromone-specific, while a major one is common in Er-1 and Er-13 crystals. A close structural inspection of this interface suggests that it may be used by Er-1 and Er-13 to associate into heterodimers, yet inapt to further associate into higher complexes. Pheromone-molecule homo-oligomerization into chains accounts for clustering and internalization of autocrine pheromone/receptor complexes in growing cells, while the heterodimer unsuitability to oligomerize may explain why heterologous pheromone/receptor complexes fail clustering and internalization. Remaining on the cell surface, they are credited with a key role in cell–cell mating adhesion.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"94 ","pages":"Article 126075"},"PeriodicalIF":2.9,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0932473924000257/pdfft?md5=82e08cb8220c05d2e4f00c51e6326a24&pid=1-s2.0-S0932473924000257-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140181978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parallel functional reduction in the mitochondria of apicomplexan parasites 螺旋体寄生虫线粒体的平行功能降低

IF 2.9 2区生物学

European journal of protistology Pub Date : 2024-03-09 DOI: 10.1016/j.ejop.2024.126065

Patrick J. Keeling , Mahara Mtawali , Morelia Trznadel , Samuel J. Livingston , Kevin C. Wakeman

{"title":"Parallel functional reduction in the mitochondria of apicomplexan parasites","authors":"Patrick J. Keeling , Mahara Mtawali , Morelia Trznadel , Samuel J. Livingston , Kevin C. Wakeman","doi":"10.1016/j.ejop.2024.126065","DOIUrl":"10.1016/j.ejop.2024.126065","url":null,"abstract":"<div>Extreme functional reduction of mitochondria has taken place in parallel in many distantly related lineages of eukaryotes, leading to a number of recurring metabolic states with variously lost electron transport chain (ETC) complexes, loss of the tricarboxylic acid (TCA) cycle, and/or loss of the mitochondrial genome. The resulting mitochondria-related organelles (MROs) are generally structurally reduced and in the most extreme cases barely recognizable features of the cell with no role in energy metabolism whatsoever (e.g., mitosomes, which generally only make iron-sulfur clusters). Recently, a wide diversity of MROs were discovered to be hiding in plain sight: in gregarine apicomplexans. This diverse group of invertebrate parasites has been known and observed for centuries, but until recent applications of culture-free genomics, their mitochondria were unremarkable. The genomics, however, showed that mitochondrial function has reduced in parallel in multiple gregarine lineages to several different endpoints, including the most reduced mitosomes. Here we review this remarkable case of parallel evolution of MROs, and some of the interesting questions this work raises.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"94 ","pages":"Article 126065"},"PeriodicalIF":2.9,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0932473924000154/pdfft?md5=2fa0d50d9ef35d502b12047cc59ed12b&pid=1-s2.0-S0932473924000154-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140129340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Minimal zoonotic risk of cryptosporidiosis and giardiasis from frogs and reptiles 蛙类和爬行动物隐孢子虫病和贾第虫病的人畜共患风险极低

IF 2.9 2区生物学

European journal of protistology Pub Date : 2024-02-28 DOI: 10.1016/j.ejop.2024.126066

Siobhon Egan , Amanda D. Barbosa , Yaoyu Feng , Lihua Xiao , Una Ryan

{"title":"Minimal zoonotic risk of cryptosporidiosis and giardiasis from frogs and reptiles","authors":"Siobhon Egan , Amanda D. Barbosa , Yaoyu Feng , Lihua Xiao , Una Ryan","doi":"10.1016/j.ejop.2024.126066","DOIUrl":"https://doi.org/10.1016/j.ejop.2024.126066","url":null,"abstract":"<div>The zoonotic potential of the protist parasites Cryptosporidium spp. and Giardia duodenalis in amphibians and reptiles raises public health concerns due to their growing popularity as pets. This review examines the prevalence and diversity of these parasites in wild and captive amphibians and reptiles to better understand the zoonotic risk. Research on Giardia in both groups is limited, and zoonotic forms of Cryptosporidium or Giardia have not been reported in amphibians. Host-adapted Cryptosporidium species dominate in reptiles, albeit some reptiles have been found to carry zoonotic (C. hominis and C. parvum) and rodent-associated (C. tyzzeri, C. muris and C. andersoni) species, primarily through mechanical carriage. Similarly, the limited reports of Giardia duodenalis (assemblages A, B and E) in reptiles may also be due to mechanical carriage. Thus, the available evidence indicates minimal zoonotic risk associated with these organisms in wild and captive frogs and reptiles. The exact transmission routes for these infections within reptile populations remain poorly understood, particularly regarding the importance of mechanical carriage. Although the risk appears minimal, continued research and surveillance efforts are necessary to gain a more comprehensive understanding of the transmission dynamics and ultimately improve our ability to safeguard human and animal health.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"93 ","pages":"Article 126066"},"PeriodicalIF":2.9,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0932473924000166/pdfft?md5=f2d522799f5a06cf61e0671be64349cf&pid=1-s2.0-S0932473924000166-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140031379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redescription and molecular phylogeny of Trochilia sigmoides Dujardin, 1841 (Ciliophora, Cyrtophoria) collected from South Korea 从韩国采集的 Trochilia sigmoides Dujardin, 1841（纤毛虫，Cyrtophoria）的重新描述和分子系统发育

IF 2.9 2区生物学

European journal of protistology Pub Date : 2024-02-28 DOI: 10.1016/j.ejop.2024.126067

Ji Hye Choi , Atef Omar , Jae-Ho Jung

{"title":"Redescription and molecular phylogeny of Trochilia sigmoides Dujardin, 1841 (Ciliophora, Cyrtophoria) collected from South Korea","authors":"Ji Hye Choi , Atef Omar , Jae-Ho Jung","doi":"10.1016/j.ejop.2024.126067","DOIUrl":"https://doi.org/10.1016/j.ejop.2024.126067","url":null,"abstract":"<div>During a survey of Korean marine ciliates, Trochilia sigmoides, the type species of the genus Trochilia, was collected and examined using in vivo observation and protargol impregnation. Moreover, scanning electron microscopy and 18S rRNA gene sequencing have been applied for the first time to study this species. Morphologically, T. sigmoides is characterized by the small body size, the oval body outline, and the spiral dorsal ridges. The Korean population of T. sigmoides shows only minute differences to other populations reported in the literature, mainly in body size and the number of dorsal ridges. Phylogenetic analyses based on 18S rRNA gene sequences show that T. sigmoides and T. petrani are placed together with two members of the family Kyaroikeidae, causing the family Dysteriidae to be non-monophyletic. The present new data increase the knowledge about the morphology and phylogeny of the genus Trochilia and would assist in understanding the phylogenetic relationship between the free-living Dysteriidae and the parasitic Kyaroikeidae.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"93 ","pages":"Article 126067"},"PeriodicalIF":2.9,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140042490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new centrohelid heliozoan, Pterocystis polycristalepis sp. nov., and taxonomic and phylogenetic concerns within Pterista (Haptista: Centroplasthelida) 一种新的中心螺旋体 Pterocystis polycristalepis sp.

IF 2.9 2区生物学

European journal of protistology Pub Date : 2024-02-20 DOI: 10.1016/j.ejop.2024.126064

Dmitry G. Zagumyonnyi, Denis V. Tikhonenkov

{"title":"A new centrohelid heliozoan, Pterocystis polycristalepis sp. nov., and taxonomic and phylogenetic concerns within Pterista (Haptista: Centroplasthelida)","authors":"Dmitry G. Zagumyonnyi, Denis V. Tikhonenkov","doi":"10.1016/j.ejop.2024.126064","DOIUrl":"10.1016/j.ejop.2024.126064","url":null,"abstract":"<div>A new species of centrohelid heliozoans, Pterocystis polycristalepis sp. nov. (Pterocystidae), was examined using light and electron microscopy. The novel centrohelid is characterized by the presence of leaf-like spine-scales with a broad pedicel-like structure on the proximal part and many subparallel ribs on the lateral wing surface. The plate-scales are ovoid with medial tubular thickening and many subparallel ribs on the very extensive marginal rim. The closely related species Pterocystis striata has also been studied in detail using light and electron microscopy. Phylogenetic analysis of 18S rRNA gene sequences placed both species into a separate clade within Pterista. The closest morphologically characterized species to the new clade is Triangulopteris lacunata. The 18S rRNA sequence of Pseudoraphidiophrys veliformis was grouped within Pterista and found to be closely related to Pterocystis polycristalepis, Pterocystis striata, and Triangulopteris lacunata. Cyst-scales of various shapes, cell and cyst aggregations, syncytia, and a cell with a stalk were revealed in a clonal culture of P. veliformis. Analysis of the morphology and phylogenetic position of the studied species and other centrohelids revealed a large number of taxonomic and phylogenetic problems in Pterista.</div>","PeriodicalId":12042,"journal":{"name":"European journal of protistology","volume":"94 ","pages":"Article 126064"},"PeriodicalIF":2.9,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139927991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rumen ciliates (Ciliophora, Trichostomatia) in Brazilian domestic cattle feeding on diets with crescent urea levels 巴西家养牛新月形尿素水平饲粮中的瘤胃纤毛虫（纤毛虫、三代寄生虫

IF 2.9 2区生物学

European journal of protistology Pub Date : 2024-02-12 DOI: 10.1016/j.ejop.2024.126063

Franciane Cedrola , Suyane Costa Bordim , Pedro Braga Arcuri , Jailton da Costa Carneiro , Roberto Júnio Pedroso Dias

引用次数: 0