European Journal of Lipid Science and Technology最新文献

{"title":"Engineered Lipid Nanoparticles beyond mRNA Vaccines: Advances in Lipid Design, Protein Corona Dynamics, and Expanding Therapeutic Horizons","authors":"Taruna Ikrar,&nbsp;Wachyudi Muchsin,&nbsp;Alfi Sophian","doi":"10.1002/ejlt.70143","DOIUrl":"https://doi.org/10.1002/ejlt.70143","url":null,"abstract":"<div>\u0000 \u0000 <p>Engineered lipid nanoparticles (LNPs) have emerged as transformative delivery vehicles for nucleic acid-based therapeutics, propelled into global prominence by the success of COVID-19 mRNA vaccines. This review consolidates advances in LNP science over the period 2019–2026 with a focus on three interrelated pillars: (i) innovations in lipid design, encompassing novel ionizable lipid architectures, biodegradable linkers, PEGylation alternatives, and artificial intelligence (AI)-driven formulation optimization; (ii) lipid metabolism and protein corona dynamics that govern biodistribution, immune recognition, and nanoparticle clearance; and (iii) expanding therapeutic applications beyond prophylactic vaccination, including CRISPR-Cas9 gene editing, cancer immunotherapy, siRNA therapeutics, rare disease treatment, and central nervous system delivery. The novelty of this review lies in its integrative, regulatory-science perspective—grounded in the mandate of a national medicines authority (Indonesia BPOM)—linking molecular lipid design to manufacturing scalability, long-term safety, and equitable access. Critical knowledge gaps including robust extrahepatic targeting, standardized protein corona characterization, and regulatory harmonization are identified and evidence-based future directions proposed. This synthesis aims to guide formulation scientists, clinician-scientists, and regulatory professionals in advancing the next generation of LNP-based precision nanomedicines.</p>\u0000 </div>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":"128 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dextrin Palmitate-Structured Sunflower Oil Oleogels for Professional Facial Massage: Evaluation of Use Potential and Safety Profile","authors":"Agnieszka Kulawik-Pióro,&nbsp;Olga Czerwińska-Ledwig,&nbsp;Anna Kurkiewicz-Piotrowska","doi":"10.1002/ejlt.70145","DOIUrl":"https://doi.org/10.1002/ejlt.70145","url":null,"abstract":"<div>\u0000 \u0000 <p>Sunflower oil, due to its favorable fatty acid profile and widespread use in cosmetic and dermatological formulations, is increasingly investigated as a base material for professional skin care products. Oleogels, through the incorporation and stabilization of active ingredients within a three-dimensional network, allow for formulation personalization and controlled release of active substances, suggesting their potential suitability for professional facial massage, including transbuccal application. This study was conducted to develop and characterize sunflower oil-based oleogels structured with dextrin palmitate and containing plant-derived macerates obtained from <i>Opuntia ficus-indica</i> and <i>Acmella oleracea</i>. Pure sunflower seed oil served as the reference. Physicochemical characterization was performed to assess formulation properties. Given the potential contact of the formulations with the oral mucosa, their safety profile was evaluated using a systematic safety assessment approach. Functional properties were determined in human volunteers using non-invasive measurements of skin hydration and transepidermal water loss (TEWL), together with glide time. Oleogelation of sunflower oil improved application properties, particularly glide time, while maintaining skin hydration and barrier-related parameters. All formulations met microbiological quality requirements, were well tolerated in dermatological testing, and showed acceptable systemic exposure dose (SED) and margin of safety (MoS) values. These findings support the potential of sunflower oil-based oleogels as safe and functional formulations for professional facial and transbuccal massage, consistent with current consumer interest in minimalist, “clean label” cosmetic products and trends toward oil-based, minimally formulated systems.</p>\u0000 <p><b>Practical Applications</b>: Knowledge of the safety profiles of cosmetic raw materials enables conscious design of innovative formulations that combine desired functional properties with a high level of user safety. The results of this study demonstrate that sunflower oil structured with dextrin palmitate and enriched with plant-derived macerates obtained from <i>Opuntia ficus-indica</i> and <i>Acmella oleracea</i> represents a safe, well-tolerated, and functional product suitable for professional facial massage, including transbuccal application. The physicochemical form of the oleogel provides an extended glide time compared to pure sunflower oil, supporting effective and comfortable massage techniques while improving skin hydration and maintaining barrier function. These findings indicate the potential for incorporating such oleogels into professional facial massage protocols, offering both enhanced application performance and a favorable safety profile.</p>\u0000 </div>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":"128 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Prediction of a Tunisian Virgin Olive Oil Based on the Effect of Climate Change Using ANFIS Method","authors":"Ines Zarrad,&nbsp;Rania Ben Hmida,&nbsp;Afif Masmoudi,&nbsp;Fakhreddine Dammak,&nbsp;Mohamed Bouaziz","doi":"10.1002/ejlt.70139","DOIUrl":"https://doi.org/10.1002/ejlt.70139","url":null,"abstract":"<p>An assessment of the interannual influence of climatic factors, namely temperature, precipitation, and humidity, on the quality of Tunisian virgin olive oil from the Zalmati cultivar was conducted using samples collected over three consecutive crop seasons (2018/2019–2020/2021), resulting in a total of 28 samples representing different ripening stages. An adaptive neuro-fuzzy inference system (ANFIS) model was developed to predict key olive oil quality parameters, including oleic acid, chlorophyll, and total phenols, based on climatic data. The ANFIS model demonstrated good predictive performance, with root mean square error (RMSE) values of 0.76, 0.87, and 12.40 during training, and 1.12, 0.79, and 24.68 during testing for oleic acid, chlorophyll, and phenols, respectively. Mean absolute percentage error (MAPE) values remained low, confirming the robustness of the model. A strong agreement between experimental and predicted results was observed. These findings demonstrate the potential of ANFIS as a reliable predictive tool for assessing climate-driven variations in olive oil composition and may support climate-adaptive strategies in olive production.</p><p><i>Practical Application</i>: This study provides a practical predictive tool for evaluating the impact of climatic variability on virgin olive oil quality under arid Mediterranean conditions. The developed ANFIS model can support olive growers and producers in anticipating changes in key quality parameters such as oleic acid, chlorophyll, and phenolic content based on climatic data. This predictive framework may assist in optimizing harvest timing, irrigation management, and crop adaptation strategies under climate change conditions. Furthermore, the model may support policymakers and agricultural stakeholders in designing sustainable management practices for olive production in vulnerable regions.</p>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":"128 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ejlt.70139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Quantification of Key Radicals in Lipid Oxidation via Spin Trapping and LC–MS/MS–IT–TOF","authors":"Yunping Yao,&nbsp;Zehui Zhang,&nbsp;Bingxin Li,&nbsp;Changmo Li","doi":"10.1002/ejlt.70142","DOIUrl":"https://doi.org/10.1002/ejlt.70142","url":null,"abstract":"<div>\u0000 \u0000 <p>Accurate qualitative and quantitative determination of free radicals during lipid oxidation, which is the core process of edible oil quality deterioration and essentially a free radical chain reaction, is of crucial importance. To overcome this problem, we developed and validated an analytical method for the specific characterization of key radicals in lipid oxidation. These radicals (R•, RO•, and ROO•) were trapped using <i>N-tert</i>-butyl-α-phenylnitrone under iron-mediated conditions, leading to the formation of stable adducts that were subsequently analyzed by LC–MS/MS–IT–TOF. This method offers good sensitivity with low limits of detection (0.06–0.15 µM) and limits of quantification (0.20–0.50 µM). Density functional theory calculations further confirm that the reaction proceeds readily and yields thermodynamically stable products. When applied to the methyl linoleate oxidation system, the method demonstrated that methyl linoleate alkyl radical (L•) predominated during oxidation, with its concentration peaking at 3.74 × 10⁻² mM (accounting for up to 70% of radicals) at 4 h followed by a decline due to chain termination reactions, whereas methyl linoleate peroxyl radicals (LOO•) concentration stabilized as oxidation progressed (1.46 × 10⁻² mM, 27% of radicals), and methyl linoleate alkoxyl radicals (LO•) remained at a consistently low concentration (1.58 × 10⁻³ mM) owing to its preferential conversion via the β-scission pathway. These results were consistent with electron spin resonance (ESR) data, thereby validating the method's reliability. Consequently, this method enables the precise monitoring of lipid oxidation processes, serving as a valuable tool for assessing antioxidant efficacy in lipid science.</p>\u0000 <p><i>Practical Applications</i>: We present an analytical method integrating <i>N-tert</i>-butyl-α-phenylnitrone (PBN)-based spin trapping with LC–MS/MS–IT–TOF for direct tracking of key lipid radicals (L•, LO•, and LOO•) throughout the lipid oxidation process. This method addresses a long-standing technical gap in lipid oxidation research, providing a robust, practical tool for both high-throughput screening of high-efficacy antioxidants and rational design of stable lipid-containing food formulations. By bridging fundamental analysis and product development, our technique has clear real-world implications for extending the shelf life of lipid-based foods, offering significant value to food chemistry research while delivering substantial economic and sustainability benefits to the global food industry.</p>\u0000 </div>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":"128 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information: Eur. J. Lipid Sci. Technol.","authors":"","doi":"10.1002/ejlt.70146","DOIUrl":"https://doi.org/10.1002/ejlt.70146","url":null,"abstract":"","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":"128 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ejlt.70146","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonpolar and Polar Lipids Composition in Recombinant Chinese Hamster Ovary Cells by Nile Red Staining Flow Cytometry","authors":"Francisco E. López-Salas,&nbsp;Enrique García-Hernández,&nbsp;Francisco Gasteazoro,&nbsp;Emiliano Balderas-Ramírez,&nbsp;Octavio T. Ramírez,&nbsp;Georgina I. López-Cortés,&nbsp;Helgi Jung-Cook,&nbsp;Emilio Rojas,&nbsp;Mauricio A. Trujillo‑Roldán,&nbsp;Norma A. Valdez‑Cruz","doi":"10.1002/ejlt.70144","DOIUrl":"https://doi.org/10.1002/ejlt.70144","url":null,"abstract":"<div>\u0000 \u0000 <p>Chinese hamster ovary (CHO) cells are the preferred system for producing recombinant proteins (RPs), and many have been used effectively in therapies. Specific productivity is related to the intrinsic genetics, biochemical, and metabolic characteristics of each producing clone. Through cell growth, lipids play a crucial role in biology. They serve as energy sources, form the bilayer of organelles and cell membranes, and are involved in the regulation of metabolic stress. To understand lipid requirements during culture, the cellular lipid composition must be considered. However, lipid studies involve complex analytical techniques, such as nuclear magnetic resonance (NMR) and mass spectrometry (MS), which require lengthy processing. Here, we explored the nonpolar and polar lipid composition in CHO cells stained with Nile red (NR), a fluorescent dye with solvatochromic properties, using flow cytometry. We describe a form to determine nonpolar and polar lipids using four different configurations of excitation wavelength laser lines and 10 different wavelength bandpass filters to acquire the fluorescence emission by flow cytometry. We show that the nonpolar and polar lipid composition changes throughout the kinetic growth of three clones of the stably transfected CHO cell line DG44 (CHO-DG44). Nonpolar lipids tend to increase in the stationary phase while polar lipids progressively decrease throughout the kinetic growth. This study provides insight into changes in lipid composition during the growth phases of CHO cells, which can be used to improve bioprocesses for CHO and other cell lines used for RP production.</p>\u0000 <p><i>Practical applications</i>: An increasing number of recombinant proteins are produced in mammalian cells. They are amply used not only in the biomedical field but also in the food industry. A necessary approach to improving the bioprocesses involved in recombinant protein production is to understand and control needed physical parameters such as temperature and oxygen consumption, as well as the required metabolites, by maintaining a continuous record of culture conditions throughout the process. This work describes a procedure for rapidly assessing changes in nonpolar and polar lipids throughout cell culture. This technique could be used to estimate lipid requirements in cell lines used in recombinant protein production and improve culture medium conditions.</p>\u0000 </div>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":"128 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in Lipid-Based Delivery Systems: A Patent Review","authors":"Darcy Lorena Junca-Fonseca,&nbsp;Luz Angela Chicuasuque Rodriguez,&nbsp;Izabel Almeida Alves,&nbsp;Mairim Russo Serafini,&nbsp;Diana Marcela Aragón","doi":"10.1002/ejlt.70141","DOIUrl":"https://doi.org/10.1002/ejlt.70141","url":null,"abstract":"<div>\u0000 \u0000 <p>Lipid-based drug delivery systems (LBDDS) are a pharmaceutical strategy that improves the bioavailability of poorly water-soluble drugs and reduces possible side effects, offering a wide variety of treatments for different pathologies, especially cancer treatment. This review aims to assess the current state of patents for LBDDS. A search was conducted in the Espacenet database from 2014 to 2024. Keywords included “Delivery system” and “Lipid”. Sixty-six patents met the inclusion criteria, originating from China, Taiwan, the United States, Japan, and South Korea. Most of these patents focus on liposomes, with the majority aimed at improving characteristics related to efficiency, safety, and cancer treatment. LBDDS have shown significant promise in improving the bioavailability, safety, and efficacy of poorly water-soluble drugs, particularly in cancer treatment and gene therapy. Patents over the past decade highlighted ongoing innovations in liposomes, nanoemulsions, and lipid-polymer hybrid nanoparticles. While academic research is outpacing patent filings, the integration of both can drive further technological advancements. Despite challenges in scalability and regulatory concerns, LBDDS continues to be a promising platform for enhancing drug delivery and therapeutic outcomes.</p>\u0000 <p><i>Practical application</i>: This patent review highlights recent technological advances in lipid-based drug delivery systems (LBDDS), offering researchers and developers a comprehensive overview of current industrial innovations. The findings support the identification of emerging excipients, nanocarrier architectures, and formulation strategies that can guide the design of next-generation lipid systems with improved stability, bioavailability, and targeting efficiency. The compiled patent trends also help innovators recognize opportunities for translational research, anticipate regulatory and manufacturing challenges, and align academic developments with industrial needs. Furthermore, the review provides insights relevant to therapeutic areas such as cancer, gene therapy, and central nervous system disorders, facilitating the strategic selection of lipid carriers for specific applications. Overall, this work serves as a valuable resource for researchers aiming to accelerate the development, optimization, and commercialization of new lipid-based pharmaceutical products.</p>\u0000 </div>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":"128 9","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148860171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palm-Oil Detection and Quantification in Adulterated Mustard Oil From Bangladesh Using ATR-FTIR Spectroscopy With Chemometric Model","authors":"Hillol Roy,&nbsp;Md. Azmain Faique,&nbsp;Saidul Islam,&nbsp;Tanu Sree Sarker,&nbsp;Tamanna Tasnim,&nbsp;Manobendra Nath Mohanta,&nbsp;Md. Mahasin Ali,&nbsp;Md. Najim Uddin,&nbsp;Md. Atikul Islam","doi":"10.1002/ejlt.70138","DOIUrl":"https://doi.org/10.1002/ejlt.70138","url":null,"abstract":"<div>\u0000 \u0000 <p>A fast and reliable approach was developed to detect and quantify palm oil in adulterated mustard oil from Bangladeshi market by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy at a spectral range of 4000–400 cm⁻¹ coupled with chemometric analysis. Orthogonal partial least squares-discriminant analysis (OPLS-DA) was used to distinguish between controlled and adulterated mustard oil, and OPLS was applied to quantify the palm-oil concentration in controlled mustard oil with good accuracy at 1210–1074 cm⁻¹. The optimal OPLS-DA model (B) obtained high cumulative <i>R</i>²<i>Y</i> and <i>Q</i>² values of 0.937 and 0.919, respectively, along with a low root mean square error of prediction (RMSEP) value of 0.146. The area under the receiver operating characteristic curve (AUROC) curve exhibited a value of 1.0, indicating the model's high accuracy in discriminating between controlled and adulterated mustard oil. The best OPLS model (P) showed strong calibration performance, with <i>R</i>²<i>X</i> (cum), <i>R</i>²<i>Y</i> (cum), and <i>Q</i>² (cum) values of 0.988, 0.985, and 0.974, along with low limit of detection (LOD) and limit of quantification (LOQ) values of 1.018 and 3.086, respectively. The validation of the selected model demonstrated good predictive performance, as evidenced by root mean square error of estimation (RMSEE) and RMSEP values of 1.899 and 2.865, respectively.</p>\u0000 <p><i>Practical Applications</i>: This study provides a non-destructive and user-friendly approach for detecting and quantifying palm-oil adulteration in mustard oil. Thus, the developed method is well-suited for routine quality monitoring in the edible oil industry.</p>\u0000 </div>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":"128 8","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Properties of Mixed Organogelators: Interactions of 9,10-Dihydroxystearic Acid and 12-Hydroxystearic Acid Towards Medium-Chain Triglyceride Oil in Organogel Systems","authors":"Norashikin Ahmad,&nbsp;Sumaiyah Megat Nabil Mohsin,&nbsp;Yusrabbil Amiyati Yusof,&nbsp;Zafarizal Aldrin Azizul Hasan,&nbsp;Suria Ramli,&nbsp;Murad Bahadi,&nbsp;Muhammad Rahimi Yusop","doi":"10.1002/ejlt.70137","DOIUrl":"https://doi.org/10.1002/ejlt.70137","url":null,"abstract":"<div>\u0000 \u0000 <p>Dihydroxystearic acid (DHSA) has shown promise as a gelling agent for structuring vegetable oils for nonfood applications. However, its use with medium-chain triglyceride (MCT) oil results in weak organogels with low gel strength and soft texture. Therefore, this study aims to improve the DHSA with MCT organogel properties by mixing DHSA with 12-hydroxystearic acid (HSA) in ratios of 100:0, 70:30, 50:50, 30:70, and 0:100 (w/w). The effects of these ratios on physicochemical characteristics, microstructure, mechanical, and thermal properties were systematically examined. An increased proportion of HSA significantly improved oil binding capacity (OBC) with DHSA/HSA, 30:70 (w/w), achieving the highest OBC (99.27%) and hardness (3739.92 g). Microscopic analysis revealed smaller crystal structures and tighter molecular packing at this ratio, contributing to the enhanced mechanical properties. X-ray diffraction and rheological studies further confirmed the formation of a stronger gel network. Fourier transform infrared analysis revealed different dimer formations in DHSA and HSA blends, whereas differential scanning calorimetry indicated a heterogeneous matrix with decreasing melting temperatures (84.5–57.9°C) as the HSA content increased. The adjustable properties of DHSA and HSA organogels offer significant potential for developing stable and durable formulations, presenting new possibilities for cosmetics and pharmaceutical applications.</p>\u0000 <p><i>Practical applications</i>: This study provides a practical strategy for tailoring the performance of medium-chain triglyceride-based organogels by combining dihydroxystearic acid and 12-hydroxystearic acid. The findings provide researchers with guidance for selecting organogelator compositions to achieve properties suited to specific nonfood applications. These design principles can support the development of customized organogel systems as oil-structuring materials for pharmaceutical, cosmetic, and other functional soft material applications requiring tailored mechanical strength, thermal behavior, and formulation stability.</p>\u0000 </div>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":"128 8","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148753791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seaweed-Derived Proteolytic Extracts as Natural Interfacial Stabilizers for Oxidatively Sensitive DHA-Rich Oil-in-Water Emulsions","authors":"Sakhi Ghelichi,&nbsp;Behdad Shokrollahi Yancheshmeh,&nbsp;Mona Hajfathalian,&nbsp;Seyed Hossein Helalat,&nbsp;Charlotte Jacobsen","doi":"10.1002/ejlt.70135","DOIUrl":"https://doi.org/10.1002/ejlt.70135","url":null,"abstract":"<p>In this work, we examined how the choice of protease in sequential enzymatic/alkaline extraction affects seaweed-derived extracts and their ability to stabilize docosahexaenoic acid (DHA) oil-in-water emulsions. Extracts produced with Alcalase (Ac-A), Flavourzyme (Fz-A), and Formea Prime (Fp-A) were compared. Ac-A achieved the highest protein extraction yield (∼95%) and phenolic content (∼17 µg GAE/mL) and showed a significantly higher ratio of essential to total amino acids (<i>p</i> &lt; 0.05). Although radical scavenging activity was similar among extracts, Ac-A exhibited stronger Fe²⁺ chelation (<i>p</i> &lt; 0.05). Ac-A and Fz-A reduced interfacial tension more effectively than Fp-A, supported by confocal laser scanning microscopy. Emulsions showed comparable droplet size (75–82 nm), negative zeta potential, and viscosity (∼1.0–1.5 centipoise [cP]) (<i>p</i> &gt; 0.05). All extracts improved oxidative stability compared to the negative control (no antioxidant); Ac-A and Fz-A showed lower peroxide value trajectories than Fp-A (<i>p</i> &lt; 0.05), whereas butylated hydroxytoluene (BHT) remained most effective. Extracts delayed tocopherol depletion and reduced volatile formation, with Ac-A performing closest to BHT. Alcalase-derived extracts thus offer multifunctional interfacial and antioxidant protection for omega-3 emulsions.</p><p><i>Practical Applications</i>: The findings support marine-derived extracts as clean-label ingredients for omega-3-enriched food products. They can be applied in systems where both physical and oxidative stability are required, such as fortified beverages, emulsified sauces, and nutraceutical formulations. Their dual functionality may reduce the need for multiple additives, simplifying ingredient lists, and improving product appeal. In addition, the use of seaweed-based materials offers opportunities for sustainable sourcing and valorization of marine biomass, which aligns with current industry trends toward natural and environment-friendly solutions.</p>","PeriodicalId":11988,"journal":{"name":"European Journal of Lipid Science and Technology","volume":"128 8","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ejlt.70135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148752872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}