{"title":"Spread of Multi-drug Resistant Aerosolised Salmonella sp and Escherichiacoli from Dumpsites in Built Environment of Abraka Town, Nigeria","authors":"F. Ihator, B. O. Ejechi","doi":"10.61448/njse213232","DOIUrl":"https://doi.org/10.61448/njse213232","url":null,"abstract":"Waste dumpsites are known to be hot spots for the development of multi-drug resistant (MDR) pathogenic microorganisms, which can be dispersed via aerosols. The dispersal of MDR Salmonella sp and E. coli in dumpsite-infested neighbourhoods of Abraka, a university town, was therefore investigated. Eosine Methylene Blue and Bismuth Sulphite agar plates were used to isolate airborne E. coli and Salmonella sp, respectively using settling plate technique. Air sampling was carried out at 3 neighbourhood dumpsites and at intervals of 50m up to 200m away from the dumpsites along the cardinal directions. The isolates were tested for antibiotics resistance using 10 antibiotics and those with multiple antibiotics resistance (MAR) index≥0.3 were considered MDR and subsequently subjected to plasmid curing, and re-tested for antibiotics resistance. The population of both organisms varied with cardinal directions and dumpsites, but generally declined as the distance from dumpsites increased (E. coli, 2.96-1.72 log cfu/m3 ; Salmonella sp, 2.65-0.00 cfu/m3 ). By overall assessment, mean MAR index for E. coli isolates was 0.39±0.01-0.64±0.04 and 0.37±0.02-0.68±0.03 for Salmonella sp isolates. MDR E. coli and Salmonella sp constituted 26.7-45.7 and 0.0-47.1% of the total population, respectively; and were encountered up to 200m away from the dumpsites at all directions although with decreasing numbers. There were significant reductions (P<0.05) in the MAR indexes of both organisms after curing thereby indicating plasmid involvement in the MDR. In conclusion, the aerosolised plasmid-encoded and chromosome-based genes in MDR organisms from dumpsites can be dispersed in neighbourhoods; and plasmids can spread antibiotics resistant genes by horizontal transfer and compound therapeutic options","PeriodicalId":119603,"journal":{"name":"Nigerian Journal of Science and Environment","volume":"62 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140485941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"WATER QUALITY ASSESSMENT OF RIVER NIGER AT ASABA/ ONITSHA AXIS, NIGERIA","authors":"O. Stephen, A. Ignatius","doi":"10.5987/UJ-NJSE.17.146.1","DOIUrl":"https://doi.org/10.5987/UJ-NJSE.17.146.1","url":null,"abstract":"The concentrations of heavy metals and selected water quality parameters of River Niger at Asaba/Onitsha axis were investigated during both dry and wet seasons in order to assess the water quality of the river. For the physicochemical properties of the water samples, the mean values ranged from 7.13 to 8.15 for pH, 227 to 749/cm for electrical conductivity, 132 to 430 mg/L for total dissolved solids and 12.3 to 13.9 mg/L for total suspended solids. The other physicochemical properties were in the ranges of (mg/L) 101 to 144, 0.06 to 0.20, 1.16 to 1.46, 6.79 to 8.48, 13.02 to 21.37 and 19.4 to 32.68 for chloride, nitrates suphate, dissolved oxygen, biochemical oxygen demand and chemical oxygen demand, respectively. The metal levels ranged from (mg/L) 6.15 to 7.69 for Fe, 0.73 to 1.15 for Zn, 0.12 to 0.22 for Cu, 0.15 to 0.02 for Mn, 0.16 to 0.22 for Ni, 0.04 to 0.06 for Cr, 0.17 to 0.26 for V, and 0.04 to 0.08 for Pb. The t-test analysis of the results revealed that there are significant variations in the values obtained for dry and wet seasons. The dry season values showed generally higher level of pollution loads. Some of the physicochemical parameters and almost all the metals investigated, showed values above Standard Organization of Nigeria (SON) and world Health Organization (WHO) permissible standard values. The extremely high values obtained for water quality index computation confirms that the River Niger water at Asaba/Onitsha axis is very unsuitable for drinking purposes.","PeriodicalId":119603,"journal":{"name":"Nigerian Journal of Science and Environment","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128238088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"COMPARATIVE STUDY OF KIDNEY LIPID PROFILE, GLUTATHIONE AND MALONDIALDEHYDE LEVELS IN MALARIA INFECTED MICE TREATED WITH AFRAMOMUM SCEPTRUM","authors":"A. A. Akpovwehwee","doi":"10.5987/UJ-NJSE.17.126.1","DOIUrl":"https://doi.org/10.5987/UJ-NJSE.17.126.1","url":null,"abstract":"The effect of aqueous extract of Aframomum sceptrum (spice) on the kidney of mice induced with malaria was assessed in this study. Six groups of six mice each were used: normal control, parasite control, normal + 250 mg of spice, parasite + 250 mg of spice, parasite+350 mg of spice and normal+350 mg of spice. After four days of treatment with aqueous extract of A. sceptrum, the mice were sacrificed and the kidneys collected to determine the levels of some biomarkers. The results revealed that the parasitized mice had a significant (p 0.05) alteration was observed in kidney level of reduced glutathione (GSH). It can be inferred that the antioxidants and bioactive metabolites of A. sceptrum are responsible for the observed change. Thus, A. sceptrum can play a crucial function in the management of malaria.","PeriodicalId":119603,"journal":{"name":"Nigerian Journal of Science and Environment","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127012535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Augustine Apiamu, Uduenevwo Evuen Francis, M. Akpan
{"title":"DETERMINATION OF POLLUTION INDEX OF BENIN RIVER FLOWING THROUGH OGHARA INDUSTRIAL SITE OF ETHIOPE WEST L.G.A, NIGERIA","authors":"Augustine Apiamu, Uduenevwo Evuen Francis, M. Akpan","doi":"10.5987/UJ-NJSE.17.128.1","DOIUrl":"https://doi.org/10.5987/UJ-NJSE.17.128.1","url":null,"abstract":"The present study was done to ascertain the pollution index of Benin River flowing through the industrial site of Oghara town for the assessment of heavy metals’ concentrations in soil and water samples. The description of the study area was enhanced by means of a geographic positioning system (GPS) and geographic information system (GIS) devices of the designated sites (A, B, C) with specific distances of 100, 200 and 300 m from the industrial site (labelled D). In each case for soil and water samples whose pHs were determined, a total of twelve samples with three samples at the respective sites were collected for the evaluation of six heavy metals (Zn, Cu, Mn, Cd, Cr and Pb) using atomic absorption spectrophotometer. Although the correlation study showed no marked significant difference (p>0.05) among the concentrations of heavy metals obtained from the designated sites, the results of pollution indices of heavy metals in soil (A-0.26, B-0.39, C-0.39 and D-0.44) and water (A-0.11, B-0.09, C-0.07 and D-0.09) samples were less than a unit, and the quality grades of the samples were considered unpolluted and may be safe for domestic and agricultural purposes.","PeriodicalId":119603,"journal":{"name":"Nigerian Journal of Science and Environment","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114601288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"IMPACT OF GAS FLARING ON BIODIVERSITY IN NIGER DELTA, NIGERIA","authors":"Ito E.E, Ugbome I.L","doi":"10.5987/UJ-NJSE.17.144.1","DOIUrl":"https://doi.org/10.5987/UJ-NJSE.17.144.1","url":null,"abstract":"Environmental pollution arising from gas flaring has impacted the biodiversity of Niger Delta. The main stresses relative to oil and gas operations arise from gas flaring, leakages of crude oil, and the escape of other chemicals used in production processes. This paper review highlighted the impacts of gas flaring on the biodiversity and the Niger delta environment. The negative effects of flaring on the flora and fauna of ecosystems have also been documented. The extinction of biodiversity, destruction and contamination of soil, and atmospheric pollution associated with gas flaring have not only deteriorated the environment, but also brought social impacts to the inhabitants of such area. Gas flaring has impoverished the communities where it is practiced, with attendant environmental, economic and health challenges. These difficulties faced by local communities and loss/threat to biodiversity from gas flares are sufficient justification for ending gas flaring practice. Fines by defaulting oil companies should be high enough to deter them while the gas can be processed and produced into cooking/domestic gas.","PeriodicalId":119603,"journal":{"name":"Nigerian Journal of Science and Environment","volume":"112 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116683953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"HYDROGEOPHYSICAL SURVEY FOR UNDERGROUD WATER IN FUGAR EDO STATE NIGERIA","authors":"Omasheye G.A.J, Onavwie Ufuoma, Ekibade Jahswill","doi":"10.5987/UJ-NJSE.17.127.1","DOIUrl":"https://doi.org/10.5987/UJ-NJSE.17.127.1","url":null,"abstract":"Twelve (12) vertical electrical sounding (VES) was done using the Schlumberger electrode array configuration to obtain data and the Schlumberger automatic analysis method of interpretation was adopted. The lithological section of the study area revealed that the area is a basement complex with a thick fresh basement beneath the surface. The weathered basement is a good accumulation for underground water due to its low resistivity values. The interpretation of the data showed a resolution of nine geoelectric layers for the VES locations and a total cumulative thicknesses of 203.55m (677.82ft), and 272.29m (906.73ft) and 27l.43m (903.83ft) depth. From the geologic explanation of the subsurface materials, these values indicate a deeper probing into the aquifer zone. This study would give a guide on effective aquifer layer position in the studied locations (Fugar, Etsako Central Local Government of Edo State).","PeriodicalId":119603,"journal":{"name":"Nigerian Journal of Science and Environment","volume":"169 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132229065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"COMPARATIVE STUDY OF THE IN VITRO ANTIOXIDANT PROPERTIES OF DIFFERENT EXTRACTS OF ETHULIA CONYZOIDES","authors":"O. Israel","doi":"10.5987/UJ-NJSE.17.131.1","DOIUrl":"https://doi.org/10.5987/UJ-NJSE.17.131.1","url":null,"abstract":"The present study was aimed to examine the in vitro antioxidant potential of Ethulia conyzoides aerial parts extracts. Different parts of E. conyzoides have been employed in traditional medicine for treating several diseases. Solvents of different polarity (petroleum ether, acetone and ethanol) were used to prepare extracts of the plant for determining their in vitro antioxidant activities using diverse in vitro assays. The antioxidant activity was measured by using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, ferric thiocyanate (FTC), reducing power and thiobarbituric acid (TBA) assay methods. Here, ascorbic acid (ASA) was used as standard antioxidant. Results obtained from the study shows that all extracts of the plant have significant scavenging activity against DPPH (45.85% for acetone, 42.00% for ethanol and 37.85% for petroleum ether extracts at 2 mg/ml each). Furthermore, the three extracts of E. conyzoides also demonstrated significant antioxidant activity in other in vitro assays with the acetone extract being the most active. Hence the present antioxidant studies of E. conyzoides, will be beneficial in the synthesis/ preparation of new drugs of pharmaceutical importance, especially its acetone extract.","PeriodicalId":119603,"journal":{"name":"Nigerian Journal of Science and Environment","volume":"351 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134399457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EFFECT OF GAMBAYA ALBIDA JUICE ON THE SURVIVABILITY OF GOAT SEMEN UNDER STORAGE IN A TROPICAL ENVIRONMENT","authors":"E. Obushe, Akporhuarho P.O, I. Udeh","doi":"10.5987/UJ-NJSE.17.138.1","DOIUrl":"https://doi.org/10.5987/UJ-NJSE.17.138.1","url":null,"abstract":"Four healthy bucks of West African Dwarf goat certified free of andrological defects were used for the experiment to determine the survivability of goat sperm in Gambaya albida juice extender under two storage conditions, namely; refrigeration temperature (5°C) and room temperature (25°C). Four storage trials were carried out for the G. albida extender using semen collected from bucks by electron-ejaculation method. Statistical analysis showed highly significant (P<0.01), effect of storage days on the motility of buck semen. The highest mortality was maintained in the first 24 h of storage, thereafter, motility decreases as the storage days increases, At the fourth day of storage motility reduced drastically at room temperature while at room temperature motility score sharply dropped to zero value. The storage temperature was recorded to be highly significant (P<0.01), except on the 6th week of trial. The refrigeration temperature was superior compare to room temperature. The refrigeration temperature maintained sperm viability for a maximum period of 96 hr. The interaction between days and storage temperature showed was highly significant (P<0.01). The storage media was significant (P<0.05); except on the 4 and 6th week of trial. The result has been able to provide the usefulness of G. albida juice in formulating semen extender for both refrigeration temperature and room temperature","PeriodicalId":119603,"journal":{"name":"Nigerian Journal of Science and Environment","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115470738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE EFFECT OF ABATTOIR WASTE WATER ON THE METABOLISM OF COWPEA SEEDLINGS GROWN IN DIESEL CONTAMINATED SOIL","authors":"F. Achuba, P. O. Erhijivwo","doi":"10.5987/UJ-NJSE.17.145.1","DOIUrl":"https://doi.org/10.5987/UJ-NJSE.17.145.1","url":null,"abstract":"The effects of abattoir waste water on the macromolecules (total sugar, protein, amino acid, beta carotene and chlorophyll) of cowpea grown on diesel contaminated soil at various concentrations (0.1%, 0.25%, 0.5%, 1.0% and 2.0%) as well as the activities of alpha amylase, starch phosphorylase and oxidative stress markers (lipid peroxidation, superoxide dismutase activity, catalase activity and xanthine oxidase) in cowpea seedlings were investigated. The results showed that diesel imposed environmental stress in cowpea seedlings. This is indicated by the decrease in total sugar, total protein and amino acids and a decrease in the chlorophyll contents of the leaves of 12-day-old seedlings. The activities of alpha amylase and starch phosphorylase in the cotyledon of 4-day-old seedlings were inhibited by the various diesel concentrations in the control treatment, but abattoir waste water ameliorated the effect of diesel toxicity. Also, the results indicated that the petroleum product caused a significant increase in lipid peroxidation and a significant decrease in the activities of the antioxidant enzymes: Superoxide dismutase, catalase and xanthine oxidase activities in the control; but abattoir waste water ameliorated the effect of these stresses posed by the diesel contaminated soil. The following observations, therefore, suggest that abattoir wastewater is capable of remediating the undesirable effects of diesel contamination on cowpea seedlings.","PeriodicalId":119603,"journal":{"name":"Nigerian Journal of Science and Environment","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128020020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"COMPARATIVE STUDY ON THE PHYTOCHEMICAL AND IN VITRO ANTIOXIDANT PROPERTIES OF METHANOLIC LEAF EXTRACT OF JUSTICIA SECUNDA VAHL","authors":"A. HamiltonAmachree, E. Osioma","doi":"10.5987/UJ-NJSE.17.139.1","DOIUrl":"https://doi.org/10.5987/UJ-NJSE.17.139.1","url":null,"abstract":"Medicinal plants are a primary source of healing and widely used for the treatment of various ailments. This present study evaluates phytochemicals and in vitro antioxidant activities of the methanolic leaf extract of Justicia secunda, Acanthaceae family. Fresh leaves of the plant were collected, and shade dried. The dry leaves were pulverized, and the resultant powder extracted with 0.1% hydrogen chloride (HCl) in methanol, and 1% HCl in methanol respectively. Extracts obtained were subjected to qualitative phytochemical screening and in vitro antioxidant assays. Results obtained confirm the presence of saponinis, tannins, steroids, flavonoids, and alkaloids in equal degree in both 0.1% and 1% HCl methanolic leaf extract. Concentrations of anthocyanins, phenols and phlobatannins were quantitatively higher in 0.1% HCl extract as compared to 1% HCl extract. However, the presence of carbohydrates was not confirmed in either extracts. The results also indicated that phenolic and anthocyanins content of the leaf extracts were significantly higher (p 0.05) in both extracts. The antioxidant scavenging efficacy showed that higher percentage inhibition was observed from the 0.1% HCl methanolic extract of Justicia secunda in its ability to scavenge hydrogen peroxide, 1,1 – diphenylpicrylhydrazyl, and superoxide anion. Overall, from this study, it could be concluded that Justicia secunda may be a good source of phytochemicals and natural antioxidants that will be useful in the food, medicine, and pharmaceutical industries.","PeriodicalId":119603,"journal":{"name":"Nigerian Journal of Science and Environment","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130774743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}