European Journal of Clinical Microbiology最新文献_第6页

Enzyme immunoassay for detection of antigen in acute Plasmodium falciparum malaria. 酶免疫分析法检测急性恶性疟原虫抗原。

European Journal of Clinical Microbiology Pub Date : 1987-10-01 DOI: 10.1007/BF02014260

B Fortier, P Delplace, J F Dubremetz, F Ajana, A Vernes

引用次数: 5

Specific and nonspecific immunodiagnostic properties of recombinant and synthetic Plasmodium falciparum antigens. 重组和合成恶性疟原虫抗原的特异性和非特异性免疫诊断特性。

European Journal of Clinical Microbiology Pub Date : 1987-10-01 DOI: 10.1007/BF02014244

J Knobloch, M Schreiber, S Grokhovsky, A Scherf

{"title":"Specific and nonspecific immunodiagnostic properties of recombinant and synthetic Plasmodium falciparum antigens.","authors":"J Knobloch, M Schreiber, S Grokhovsky, A Scherf","doi":"10.1007/BF02014244","DOIUrl":"https://doi.org/10.1007/BF02014244","url":null,"abstract":"Six Plasmodium falciparum/beta-galactosidase fusion proteins produced by a genomic DNA expression library, and two synthetic Plasmodium falciparum antigens were applied to ELISA and tested for their immunodiagnostic properties. Results were compared to reference methods, i.e. fluorescence antibody test with whole cell antigen and ELISA with detergent-soluble crude schizont antigen. Anti-Plasmodium falciparum antibodies could be detected by these molecular antigens to varying extents in human sera. Undesired reactivity to fusion proteins due to the high prevalence of antibodies to beta-galactosidase in human sera and undesired reactivity to one of the synthetic antigens (P12) frequently occurred. The antibodies responsible for the nonspecific reactivity could not be identified. It was concluded that the application of molecular Plasmodium falciparum antigens to ELISA represents a practicable approach to immunodiagnosis of malaria if the construction of epitopes that bind antibodies other than Plasmodium falciparum antibodies can be avoided.","PeriodicalId":11958,"journal":{"name":"European Journal of Clinical Microbiology","volume":"6 5","pages":"547-51"},"PeriodicalIF":0.0,"publicationDate":"1987-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02014244","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14452477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In vitro activity of four fluoroquinolones against eighty-six isolates of mycobacteria. 四种氟喹诺酮类药物对86株分枝杆菌的体外活性研究。

European Journal of Clinical Microbiology Pub Date : 1987-10-01 DOI: 10.1007/BF02014255

J Texier-Maugein, M Mormède, J Fourche, C Bébéar

引用次数: 6

Lectin-mediated bacterial adhesion to human tissue. 凝集素介导的细菌对人体组织的粘附。

European Journal of Clinical Microbiology Pub Date : 1987-10-01 DOI: 10.1007/BF02014258

J Beuth, H L Ko, G Uhlenbruck, G Pulverer

引用次数: 5

Enhanced susceptibility of trypsinized HeLa cells to Chlamydia trachomatis infection. 胰蛋白酶化HeLa细胞对沙眼衣原体感染的敏感性增强。

European Journal of Clinical Microbiology Pub Date : 1987-10-01 DOI: 10.1007/BF02014259

A O Oyelese, R C Brunham, J McDowell, T Williams

引用次数: 1

Commercially available fluorescein-conjugated monoclonal antibody for determining the in vitro activity of antimicrobial agents against Chlamydia trachomatis. 市售荧光素偶联单克隆抗体，用于测定抗沙眼衣原体抗菌药物的体外活性。

European Journal of Clinical Microbiology Pub Date : 1987-10-01 DOI: 10.1007/BF02014256

J M Webberley, R S Matthews, J M Andrews, R Wise

引用次数: 2

Enterococcal urinary tract infections in a teaching hospital. 某教学医院肠球菌性尿路感染。

European Journal of Clinical Microbiology Pub Date : 1987-10-01 DOI: 10.1007/BF02014250

L Lemoine, P R Hunter

引用次数: 6

In vitro activity of ciprofloxacin against methicillin-susceptible and methicillin-resistant staphylococci. 环丙沙星对甲氧西林敏感和耐甲氧西林葡萄球菌的体外活性研究。

European Journal of Clinical Microbiology Pub Date : 1987-10-01 DOI: 10.1007/BF02014254

B Gahrn-Hansen, P Søgaard, M Arpi

引用次数: 2

Susceptibility to beta-lactam antibiotics in septicemia isolates from twenty-nine European laboratories. European Study Group on Antibiotic Resistance. 来自29个欧洲实验室的败血症分离株对β -内酰胺类抗生素的敏感性欧洲抗生素耐药性研究小组。

European Journal of Clinical Microbiology Pub Date : 1987-10-01 DOI: 10.1007/BF02014238

{"title":"Susceptibility to beta-lactam antibiotics in septicemia isolates from twenty-nine European laboratories. European Study Group on Antibiotic Resistance.","authors":"","doi":"10.1007/BF02014238","DOIUrl":"https://doi.org/10.1007/BF02014238","url":null,"abstract":"In 1984 the European Study Group on Antibiotic Resistance (ESGAR) consecutively collected gram-negative bacilli and staphylococci blood isolates and performed susceptibility testing with 11 antibiotics using the microdilution method. In all 2,578 isolates were collected: 68% gram-negative bacilli and 32% staphylococci. The MICs of ampicillin and cefazoline for the susceptible gram-negative bacilli were 1-8 micrograms/ml; of piperacillin less than or equal to 0.5-4; of Sch 34343, cefotaxime, moxalactam, ceftazidime and aztreonam less than or equal to 0.5-2 micrograms/ml; of cefoxitin, cefuroxime and cefamandole less than or equal to 0.5-8 micrograms/ml. For susceptible staphylococci the MICs of cefazoline and cefuroxime were less than or equal to 0.5-1 micrograms/ml, and of cefoxitin, moxalactam, ceftazidime and cefotaxime, less than or equal to 0.5-32 micrograms/ml. The resistance levels varied between laboratories and countries, being lower in Northern Europe. In clinical protocols on patients with gram-negative septicemia from whom cefazoline-resistant strains were isolated, cefotaxime was the beta-lactam most commonly used (12%). In protocols on patients with staphylococcal septicemia from whom gentamicin-resistant or cefazoline-resistant strains were isolated, the most commonly used beta-lactam was cloxacillin (6%).","PeriodicalId":11958,"journal":{"name":"European Journal of Clinical Microbiology","volume":"6 5","pages":"515-20"},"PeriodicalIF":0.0,"publicationDate":"1987-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02014238","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14560705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological aspects of Clostridium difficile in a pediatric hospital and its role in diarrheal disease. 儿科医院艰难梭菌的流行病学特征及其在腹泻病中的作用。

European Journal of Clinical Microbiology Pub Date : 1987-10-01 DOI: 10.1007/BF02014243

M Camorlinga-Ponce, M Gamboa, J J Barragan, O Munoz, F R Fekety, J F Torres

{"title":"Epidemiological aspects of Clostridium difficile in a pediatric hospital and its role in diarrheal disease.","authors":"M Camorlinga-Ponce, M Gamboa, J J Barragan, O Munoz, F R Fekety, J F Torres","doi":"10.1007/BF02014243","DOIUrl":"https://doi.org/10.1007/BF02014243","url":null,"abstract":"The influence of antibiotics on the frequency of colonization by Clostridium difficile and the presence of its cytotoxin in infants and older children was examined to determine its role in diarrheal disease. Cytotoxin was more closely associated with cases of diarrhea, both in infants and in children than the microorganism, although not significantly. The isolates were typed by means of sensitivity to bacteriophages and bacteriocins and their cytotoxigenic potential was also determined. Less than 30% of the colonized patients had toxigenic strains. A study of strain variability over a four-year period in the same hospital showed that two bacteriophage-bacteriocin types and non-toxigenic strains predominated. The common presence of non-toxigenic strains could explain in part the lack of correlation between isolation of Clostridium difficile and diarrhea. Most of the non-toxigenic strains showed moderate resistance to tetracycline, a property which might explain their ability to persist for long periods in the hospital.","PeriodicalId":11958,"journal":{"name":"European Journal of Clinical Microbiology","volume":"6 5","pages":"542-6"},"PeriodicalIF":0.0,"publicationDate":"1987-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02014243","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14560707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8