Commercially available fluorescein-conjugated monoclonal antibody for determining the in vitro activity of antimicrobial agents against Chlamydia trachomatis.

J M Webberley, R S Matthews, J M Andrews, R Wise
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引用次数: 2

Abstract

A simple, reproducible method for determining the antibiotic susceptibility of chlamydial isolates is described. Minimum inhibitory and lethal concentrations (MICs and MLCs) were determined for tetracycline and erythromycin titrated against a recent clinical isolate of Chlamydia trachomatis in McCoy cell cultures. A fluorescent antibody stain was found to be more sensitive than giemsa staining, generally giving two-fold higher values for both MICs and MLCs.

市售荧光素偶联单克隆抗体,用于测定抗沙眼衣原体抗菌药物的体外活性。
本文描述了一种测定衣原体分离物抗生素敏感性的简单、可重复的方法。测定了四环素和红霉素对McCoy细胞培养中最近临床分离的沙眼衣原体的最小抑制浓度和致死浓度(mic和MLCs)。发现荧光抗体染色比吉姆萨染色更敏感,通常对mic和MLCs的值都高两倍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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