Equine Veterinary Journal最新文献

{"title":"Yearling laryngeal function grades II.2 and below are not associated with reduced performance.","authors":"Josephine L Hardwick, Benjamin J Ahern, Kylie L Crawford, Kate J Allen, Brian H Anderson, Kim J Rose, Samantha H Franklin","doi":"10.1111/evj.14452","DOIUrl":"https://doi.org/10.1111/evj.14452","url":null,"abstract":"<p><strong>Background: </strong>The relationship between Thoroughbred yearling laryngeal function (YLF) grade and race performance is unclear.</p><p><strong>Objectives: </strong>To determine the effect of YLF on future race performance.</p><p><strong>Study design: </strong>Retrospective cohort study.</p><p><strong>Methods: </strong>Post-sale endoscopic recordings were reviewed from Australian yearling sales in 2018-2019. Race performance was evaluated for career and as 2-, 3- and ≥4-year-olds. Multivariable generalised linear modelling examined the association between YLF and performance, with risk estimates presented as coefficients (95% CI).</p><p><strong>Results: </strong>The YLF in 5175 examinations was graded I in 29.8% (n = 1542); II.1 in 49.0% (n = 2537), II.2 in 16.5% (n = 855), III.1 in 3.9% (n = 200), III.2 in 0.8% (n = 39) and III.3 in <0.04% (n = 2). Additional endoscopic abnormalities included ventroaxial luxation of the corniculate process (VLAC, n = 77, 1.5%); arytenoid mucosal lesions (n = 392, 7.6%) and intermittent dorsal displacement of the soft palate (iDDSP, n = 1264, 24.4%). Median (IQR) career earnings were: grade I $45 095 ($15 565, $113 220); grade II.1 $45 315 ($15 915, $107 490), grade II.2 $38 610 ($14 326, $95 218), grade III.1 $32 765 ($8565, $86 030) and grade III.2 $35 810 ($3700, $65 770). There was no difference in career earnings for YLF grades II.2 and III.1, compared with referent grade I/II.1, whereas grade III.2 earned less overall (-$46 015 (95% CI: -$89 994, -$2036), p = 0.04). Earnings in ≥4-year-olds were less for grades III.1 (-$35 076 (-$56 129, -$14 024), p = 0.001) and III.2 (-$53 219, (-$76 062, -$30 375) p < 0.001).</p><p><strong>Main limitations: </strong>Lack of follow-up data due to retrospective nature of study. Exclusion of unraced horses and those with no prize money from analysis.</p><p><strong>Conclusions: </strong>Ninety-five percent of the yearling population had grades I, II.1 or II.2 YLF and minimal difference in race performance was identified between them. Horses with grade III.1 YLF performed similarly to grades I/II.1 in their early careers but had reduced race performance at ≥4-year-old.</p>","PeriodicalId":11796,"journal":{"name":"Equine Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of equine odontoclastic tooth resorption and hypercementosis: A comparative study using microCT and radiography in age-matched controls.","authors":"Zoe Nugent, Anders Jensen, Niamh Owen, Andrew J Peffers, Mohesh Moothanchery, Mandy J Peffers","doi":"10.1111/evj.14453","DOIUrl":"https://doi.org/10.1111/evj.14453","url":null,"abstract":"<p><strong>Background: </strong>Equine odontoclastic tooth resorption and hypercementosis (EOTRH) is a painful disorder primarily affecting the incisor teeth of horses over 15 years of age. Clinical signs of the disease include prehension problems, halitosis and in severe cases weight loss. The disease predominately affects the reserve crown and presents as a loss of dental tissue and excessive build-up of cementum.</p><p><strong>Objectives: </strong>To determine the radiographic scores of horses with EOTRH and age-matched controls and to increase understanding of EOTRH using microCT to compare teeth from horses with EOTRH and age-matched controls.</p><p><strong>Study design: </strong>In vivo and ex vivo studies.</p><p><strong>Methods: </strong>This study used radiography (in vivo) and microcomputed tomography (microCT) (ex vivo) to help understand and help characterise the imaging changes that occur in EOTRH. For radiography, 87 patients were assessed using a radiographic scoring system for EOTRH. The microCT study was undertaken on 20 extracted cadaver incisor teeth which were scanned and segmented to measure the different dental tissues. These were assessed using a descriptive analysis (surface roughening, tooth resorption, root blunting and pulp cavity).</p><p><strong>Results: </strong>Radiographic scoring demonstrated that 03s were more severely affected than 01s in EOTRH. Total radiographic score and age had a weak positive correlation. Following microCT, we identified that EOTRH teeth had a lower pulp and enamel volume and therefore significantly higher pulp and enamel ratios relative to the whole tooth volume, compared with control teeth. Cementum and dentine volumes were more variable in EOTRH teeth. Thus, their ratios relative to the whole tooth volume were not different to control teeth.</p><p><strong>Main limitations: </strong>The number of horses was relatively small.</p><p><strong>Conclusions: </strong>Results suggest differing degrees of tooth resorption and hypercementosis, in different affected teeth potentially indicating multiple phenotypes of the disease. We provide further evidence of the presence of subclinical EOTRH imaging changes in some teeth.</p>","PeriodicalId":11796,"journal":{"name":"Equine Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of experimentally induced endotoxaemia and meloxicam administration on the haemostatic system in donkeys.","authors":"Alejandro Perez-Ecija, Antonio Buzon-Cuevas, Carlos Gonzalez-De Cara, Raul Aguilera-Aguilera, Adelaida de Las Heras, Francisco J Mendoza","doi":"10.1111/evj.14476","DOIUrl":"https://doi.org/10.1111/evj.14476","url":null,"abstract":"<p><strong>Background: </strong>Endotoxaemia is a common condition in equids, frequently accompanied by alterations in haemostasis. Non-steroidal anti-inflammatory drugs, such as meloxicam, have been proven to alleviate some signs of endotoxaemia in donkeys. Neither the haemostatic response to induced endotoxaemia nor the effect of meloxicam in this regard have been described in donkeys.</p><p><strong>Objectives: </strong>(a) To characterise the haemostatic changes in response to induced endotoxaemia in healthy donkeys, and (b) to assess the effect of meloxicam in these animals.</p><p><strong>Study design: </strong>In vivo experiments.</p><p><strong>Methods: </strong>Endotoxaemia was induced by LPS (20 ng/kg) infusion in six healthy adult Andalusian donkeys. All animals randomly received either an IV bolus of saline solution (untreated group) or meloxicam (0.6 mg/kg) after LPS infusion (PLI), with a 1-month washout period between trials. Samples were collected at -30, 0, 30, 60, 90, 120, 180 and 360 min PLI for clotting times, antithrombin III, anti-Xa activities, platelet and fibrinogen concentrations; and at -30, 90, 180 and 360 min PLI for viscoelastography (Sonoclot).</p><p><strong>Results: </strong>LPS caused prothrombotic-specific viscoelastographic changes (short activated clot time and elongated clot rate formation) and lower antithrombin III activities compared with reference range for this species. Prolonged prothrombin times were also seen at late timepoints in the experiment. Meloxicam administration resulted in no significant differences between groups.</p><p><strong>Main limitations: </strong>Short sampling duration, only Andalusian donkeys.</p><p><strong>Conclusions: </strong>Acute endotoxaemia induced hypercoagulability and initial signs of a consumptive coagulopathy in donkeys. Despite a more prolonged increase in anti-Xa activity, meloxicam did not ameliorate most of these haemostatic changes.</p>","PeriodicalId":11796,"journal":{"name":"Equine Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mare colostrum quality and relationship with foal serum immunoglobulin G concentrations and average daily weight gains.","authors":"Kirsty Gallacher, Katherine Champion, Katharine S Denholm","doi":"10.1111/evj.14471","DOIUrl":"https://doi.org/10.1111/evj.14471","url":null,"abstract":"<p><strong>Background: </strong>Foals suffer from total failure to transfer passive immunity (TFTPI) when serum immunoglobulin (IgG) is <4 g/L, and partial failure to transfer passive immunity (PFTPI) when serum IgG is 4-8 g/L.</p><p><strong>Objectives: </strong>To explore risk factors for poor serum IgG concentration.</p><p><strong>Study design: </strong>Retrospective observational study.</p><p><strong>Methods: </strong>Data from 535 Thoroughbred foals born to 177 mares were analysed and included foal sex, birthweight, month and year of birth and colostrum Brix %. Associations between dam age and colostrum Brix (%); and between foal serum IgG and liveweight gains were also measured. Pre-suckle colostrum samples and foal blood samples were collected (by jugular venipuncture within 12-24 h of birth). IgG was estimated in mare colostrum and foal serum using Brix refractometry and turbidimetric immunoassay, respectively. Foals were weighed within 20 h of birth; daily until 7 days of age and weekly until 130 days of age.</p><p><strong>Results: </strong>Mean foal serum IgG was 10.78 g/L (SD = 3.26) and mean mare colostrum Brix was 27.32% (SD = 5.96). A number of colostrum samples (20.9%, n = 112/535) measured <20% Brix and 20.4% of foals (n = 109/535) had serum IgG concentrations ≤8 g/L, 2.2% of foals (n = 12/535) had serum IgG less than or equal to 4 g/L and 18.1% (n = 97/535) had serum IgG between 4.1 g/L and 8 g/L. Foals had an average daily gain (ADG, kg) of 1.26 kg (SD = 0.14). Serum IgG (g/L) was associated with year of birth, birthweight (kg) and colostrum Brix (%). For every unit increase in foal birthweight (kg), there were small, significant increases in foal serum IgG (0.04 g/L, p = 0.04). Similarly, for every unit increased in mare colostrum Brix (%) there were small, significant increases in foal serum IgG (0.25 g/L, p < 0.001). Month of birth was significantly associated with colostrum Brix (%) and with average daily gains; with lower values for both outcome parameters with increasing calendar month. Increasing dam age at foaling was associated with lower colostrum Brix (%).</p><p><strong>Main limitations: </strong>Retrospective design with missing data, small convenience sample.</p><p><strong>Conclusions: </strong>Several risk factors were significantly associated with foal serum IgG and mare colostrum Brix (%) in the current work.</p>","PeriodicalId":11796,"journal":{"name":"Equine Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Report from the 2024 Dorothy Russell Havemeyer Working Group Meeting on Consensus Definitions for Foal Sepsis.","authors":"P A Wilkins, D M Wong","doi":"10.1111/evj.14443","DOIUrl":"https://doi.org/10.1111/evj.14443","url":null,"abstract":"","PeriodicalId":11796,"journal":{"name":"Equine Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A genome-wide association study for recurrent laryngeal neuropathy in the Thoroughbred horse identifies a candidate gene that regulates myelin structure.","authors":"Charlotte L McGivney, Beatrice A McGivney, Gabriella Farries, Katie F Gough, Haige Han, Amy R Holtby, David E MacHugh, Lisa Michelle Katz, Emmeline W Hill","doi":"10.1111/evj.14461","DOIUrl":"https://doi.org/10.1111/evj.14461","url":null,"abstract":"<p><strong>Background: </strong>Equine recurrent laryngeal neuropathy (RLN) is an economically important upper respiratory tract (URT) disease with a genetic contribution to risk, but genetic variants independent of height have not been identified for Thoroughbreds. The method of clinical assessment for RLN is critical to accurately phenotype groups for genetic studies.</p><p><strong>Objectives: </strong>To identify genetic risk loci for RLN in Thoroughbreds in a genome-wide association study (GWAS) following high-resolution phenotyping.</p><p><strong>Study design: </strong>Case-control.</p><p><strong>Methods: </strong>Thoroughbred horses were characterised as RLN cases and controls using resting and exercising URT endoscopic examinations and laryngeal ultrasonography, with the case-cohort supplemented using a questionnaire. Genotypes for 43 831 autosomal single-nucleotide polymorphisms (SNPs) from n = 235 horses (n = 110 cases; n = 125 controls) were used to estimate trait heritability and identify significantly associated SNPs in a GWAS. Haplotypes were examined in cases and controls and risk allele frequencies were examined in a population cohort (n = 3126).</p><p><strong>Results: </strong>Heritability was h² = 0.30 including sex and 5PCs as covariates. A SNP on ECA20 located between candidate genes, DAAM2 and LRFN2, was significantly associated with RLN. Six index SNPs with allelic effect sizes OR = 1.5-2.9 were identified on ECA1, ECA14, and ECA20 close to candidate genes ATPA10, KCNN2, and TFAP2A. Eleven ECA20 SNPs defined seven haplotypes with homozygous H2/H2 horses having a 3.1× higher risk of RLN. Risk alleles segregate in the population, and stallions are carriers.</p><p><strong>Main limitations: </strong>The main study population was young. Horses in the control group had no evidence of RLN as 2- or 3-year olds but may have developed RLN later.</p><p><strong>Conclusions: </strong>Genetic markers for RLN were identified which may be useful for the development of a polygenic risk score. Candidate genes with functions in neuropathies may further the understanding of RLN pathobiology.</p>","PeriodicalId":11796,"journal":{"name":"Equine Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors influencing equine veterinarians' job satisfaction and retention: A focus group study.","authors":"Kristen Whitaker, Audrey Burnette, Jean-Yin Tan, Meggan Graves, Julie Hunt, Elizabeth Devine, Stacy Anderson, Katherine Kirkendall, Lauren Wisnieski","doi":"10.1111/evj.14467","DOIUrl":"https://doi.org/10.1111/evj.14467","url":null,"abstract":"<p><strong>Background: </strong>There is a shortage of equine veterinarians. Understanding what factors are associated with job satisfaction in equine veterinarians can inform interventions to increase retention in equine medicine.</p><p><strong>Objective: </strong>To explore the prominent factors causing work dissatisfaction and burnout in equine veterinarians.</p><p><strong>Study design: </strong>Qualitative research study consisting of semi-structured focus groups.</p><p><strong>Methods: </strong>Thirty-seven current and former equine veterinarians across the United States were recruited via snowball and convenience sampling to answer questions on work history, work-life balance, and perceptions of equine practice. Transcripts were analysed in Delve and coded in the context of the Conservation of Resources (COR) theory. A card sorting activity was used to rank the four types of resources in the COR theory (condition, object, energy, and personal).</p><p><strong>Results: </strong>Condition resources were the most frequently mentioned reasons for work dissatisfaction. These included issues with discrimination or bias due to age, race/ethnicity, and gender, unpredictable and long hours, and heavy workloads. Object resources, such as equipment, were rarely mentioned. Energy resources, including pay and student loan debt, were influential, with most participants feeling that equine veterinarians are underpaid. Personal resources, such as problem-solving skills and enjoyment in helping others, improved job satisfaction.</p><p><strong>Main limitations: </strong>Although recruiting efforts prioritised perspectives of black, indigenous, and people of colour, lesbian, gay, bisexual, transgender, queer plus identities, and members with disabilities, demographic information was not directly collected.</p><p><strong>Conclusions: </strong>The main barriers to equine veterinary retention included a lack of work-life balance, long hours, lower-than-expected pay, and issues with discrimination and bias. This study highlights areas for intervention to improve the equine veterinary field, such as higher pay, rural practice incentives, and effective diversity, equity, inclusion, and belonging (DEIB) efforts. A shift toward caseload-sharing between veterinarians could help alleviate excessive emergency on-call and burnout.</p>","PeriodicalId":11796,"journal":{"name":"Equine Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in pressure distribution of the solar surface after a single trimming event are associated with external hoof measurements in the equine fore foot.","authors":"Sarah Seery, James Gardiner, Karl T Bates, Gina Pinchbeck, Pete Clegg, Joanne L Ireland, Peter I Milner","doi":"10.1111/evj.14463","DOIUrl":"https://doi.org/10.1111/evj.14463","url":null,"abstract":"<p><strong>Background: </strong>Trimming is critical for a functioning equine hoof. Pressure distribution provides information on loading; however, information on the effects of trimming on pressure distribution is lacking.</p><p><strong>Objectives: </strong>To describe the pressure changes of equine fore feet following trimming.</p><p><strong>Study design: </strong>Cross-sectional cohort study.</p><p><strong>Methods: </strong>Fifty sound horses were recruited. Eighteen external hoof measures of the dorsal, lateral, medial and solar aspects were obtained before and after trimming from 94 fore feet. Horses were walked over a pressure mat before and after trimming and pressure maps of the solar surface created. Percentage change in hoof measures were assessed. Factors associated with an increase in pressure in the frog region after trimming were entered into a forward likelihood ratio logistic regression model. Odd ratios (ORs) with 95% confidence intervals (CI) and area under the curve receiver operator characteristics (AUROC) were calculated. Sensitivity and specificity were calculated at a cut-off value of p = 0.5.</p><p><strong>Results: </strong>Trimming resulted in a significant increase in pressure, topographically mapped to the frog region, in 12/94 (13% 95% CI 6; 20) feet. Percentage difference in bearing border length (OR 0.66 95% CI 0.51; 0.86), heel buttress to centre of pressure distance (OR 1.30 95% CI 1.10; 1.53), heel angle (lateral side) (OR 1.11 95% CI 1.04; 1.19) and heel length (medial side) (OR 0.92 95% CI 0.85; 0.99) were retained in the final model associated with increased pressure in the frog region following trimming. AUROC was excellent (0.94 95% CI 0.88; 0.99) with fair sensitivity (58% [95% CI 50; 66]) and excellent specificity (98% [95% CI 78; 118]).</p><p><strong>Main limitations: </strong>Subjective lameness exam; horse velocity unmeasured.</p><p><strong>Conclusions: </strong>Measuring pressure changes over the solar surface of the equine fore foot after trimming identified that an increased pressure in the frog region was linked to specific changes in hoof shape.</p>","PeriodicalId":11796,"journal":{"name":"Equine Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammation and response to bacterial infection as potential drivers of equine odontoclastic tooth resorption and hypercementosis: A proteomics insight.","authors":"Anders Jensen, Emily J Clarke, Zoe Nugent, Emily Paice, Iris Gringel, Kazuhiro Yamamoto, Guido Rocchigiani, Andrew J Peffers, Lee Cooper, Mandy J Peffers","doi":"10.1111/evj.14469","DOIUrl":"https://doi.org/10.1111/evj.14469","url":null,"abstract":"<p><strong>Background: </strong>Equine dental diseases significantly impact a horse's overall health, performance and quality of life. They can result in secondary infections and digestive disturbances, potentially leading to colic. A recently described disease affecting the incisors of horses is equine odontoclastic tooth resorption and hypercementosis (EOTRH). Understanding EOTRH is crucial for early diagnosis, effective management and prevention of its severe consequences.</p><p><strong>Objectives: </strong>To determine proteomic differences in incisor cementum in horses with and without clinical EOTRH.</p><p><strong>Study design: </strong>Comparative and observational clinical study.</p><p><strong>Methods: </strong>Teeth were extracted (N = 5) and cementum was isolated using a diamond wire. Proteins were extracted using an optimised sequential workflow, and trypsin was digested for mass spectrometry. Protein identification and label-free quantification were undertaken.</p><p><strong>Results: </strong>In total 1149 unique proteins were detected in cementum across all samples. We identified four proteins exclusively in EOTRH-affected cementum. EOTRH samples showed a higher heterogeneity than healthy samples. In total, 54 proteins were increased in EOTRH, and 64 proteins were reduced (adjusted p-value <0.05). Inflammatory proteins, such as cathepsin G (p = 0.004), neutrophil elastase (p = 0.003), bactericidal permeability-increasing protein (p = 0.002), azurocidin (p = 0.003) and lactotransferrin (p = 0.002) were all increased in EOTRH. Pathway analysis revealed that antimicrobial peptides (Z score 2.65, p = 1.93E-09) and neutrophil degranulation (Z-score 1.89, p = 1.7E-04) were commonly up-regulated canonical pathways.</p><p><strong>Main limitations: </strong>The sample size was limited. Lack of age-matched healthy controls.</p><p><strong>Conclusion: </strong>EOTRH leads to biochemical changes within the cementum proteome, which are important in explaining the physiological changes occurring in disease. Differentially abundant proteins may represent promising biomarkers for earlier disease detection and the establishment of a cell-based model could provide further insight into the role these proteins play in hypercementosis and resorption.</p>","PeriodicalId":11796,"journal":{"name":"Equine Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterisation of temporal abundance of microRNAs in synovial fluid from an experimental equine model of osteoarthritis.","authors":"Marie Walters, Kerstin Skovgaard, Peter M H Heegaard, Yongxiang Fang, Yalda A Kharaz, Louise Bundgaard, Lene T Skovgaard, Henrik E Jensen, Pia H Andersen, Mandy J Peffers, Stine Jacobsen","doi":"10.1111/evj.14456","DOIUrl":"https://doi.org/10.1111/evj.14456","url":null,"abstract":"<p><strong>Background: </strong>MicroRNAs, a class of small noncoding RNAs, serve as post-transcriptional regulators of gene expression and are present in a stable and quantifiable form in biological fluids. MicroRNAs may influence intra-articular responses and the course of disease, but very little is known about their temporal changes in osteoarthritis.</p><p><strong>Objectives: </strong>To identify miRNAs and characterise the temporal changes in their abundance in SF from horses with experimentally induced osteoarthritis. We hypothesised that the abundance of miRNA would change during disease progression.</p><p><strong>Study design: </strong>In vivo experiments.</p><p><strong>Methods: </strong>RNA extracted from synovial fluid obtained sequentially (Day 0, 28 and 70) from nine horses with experimentally induced osteoarthritis was subjected to small RNA sequencing using the Illumina Hiseq 4000 sequencing platform. Differentially abundant miRNAs underwent further validation and mapping of temporal abundance (Day 0, 14, 17, 21, 28, 35, 42, 49, 56, 63 and 70 days after osteoarthritis induction) by microfluidic reverse transcription quantitative real-time PCR. Bioinformatic analyses were performed to predict potential biological associations and target genes of the differentially abundant microRNAs.</p><p><strong>Results: </strong>Small RNA sequencing revealed 61 differentially abundant microRNAs at an early osteoarthritis stage (Day 28), and subsequent reverse transcription quantitative real-time PCR analysis validated 20 of these. Significant biological functions of the differentially abundant microRNAs were apoptosis, necrosis, cell proliferation and cell invasion. Following validation, four microRNAs (miRNA-199b-3p, miRNA-139-5p, miRNA-1839 and miRNA-151-5p) were detected in more than 50% of the synovial fluid samples and had higher abundance in osteoarthritic than in control joints.</p><p><strong>Main limitations: </strong>Limited sample size.</p><p><strong>Conclusion: </strong>This is the first study to determine longitudinal changes in synovial fluid microRNA abundance in an equine model of osteoarthritis. Larger studies are needed in naturally occurring osteoarthritis to interrogate putative changes identified by this study.</p>","PeriodicalId":11796,"journal":{"name":"Equine Veterinary Journal","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142946658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}