European Journal of Clinical Microbiology & Infectious Diseases最新文献

{"title":"Investigation of antimicrobial susceptibility and resistance gene prevalence in Capnocytophaga spp. isolated from dogs and cats and characterization of novel class A β-lactamase CST-1.","authors":"Kaoru Umeda, Michio Suzuki, Koichi Imaoka","doi":"10.1007/s10096-024-05025-0","DOIUrl":"10.1007/s10096-024-05025-0","url":null,"abstract":"<p><strong>Purpose: </strong>Capnocytophaga spp., common inhabitants of the animal oral cavity, are zoonotic pathogens transmitted to humans through dog/cat bites and cat scratches. Appropriate antimicrobial therapy is essential for treatment this zoonotic disease because of the rapid deterioration of systemic symptoms at disease onset; however, antimicrobial resistance of animal bite-associated Capnocytophaga spp. has not been fully investigated. We sought to understand the antimicrobial susceptibility and prevalence of resistance genes among Capnocytophaga sp. isolates obtained from dogs and cats.</p><p><strong>Method: </strong>Minimum inhibitory concentrations (MICs) of nine antibiotics for a total of 57 isolates belonging to 6 species (C. canimorsus, C. cynodegmi, C. canis, C. felis, C. stomatis, and C. catalasegens) were assayed using E-test. Resistance genes were detected using polymerase chain reaction, nucleotide sequencing, and whole-genome sequencing.</p><p><strong>Results: </strong>The MICs of penicillin, ceftriaxone, cefepime, clindamycin, minocycline, nalidixic acid, and ciprofloxacin were high for some isolates. The MICs of imipenem and amoxicillin/clavulanic acid were low for all isolates. Known resistance genes bla_cfxA2, bla_OXA-347, emrF, and tetQ were detected using polymerase chain reaction. Mutation in the quinolone resistance-determining region of gyrA was also detected. Cst-1, a previously unreported gene, was identified using whole-genome analysis of two C. stomatis isolates. CST-1 was proposed as a class A, subclass A2, β-lactamase based on amino acid sequence and phylogenetic relationship. In recombination experiments, CST-1 inactivated penicillin and first- and second-generation cephems; however, sulbactam inhibited it.</p><p><strong>Conclusion: </strong>Known and novel resistance genes are prevalent among Capnocytophaga spp. in animal oral cavities. The findings have clinical implications, especially in antimicrobial treatment.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"559-569"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple regional outbreaks caused by global and local VIM-producing Klebsiella pneumoniae clones in Poland, 2006-2019.","authors":"Marta Biedrzycka, Paweł Urbanowicz, Sylvain Brisse, Federica Palma, Dorota Żabicka, Marek Gniadkowski, Radosław Izdebski","doi":"10.1007/s10096-024-05016-1","DOIUrl":"10.1007/s10096-024-05016-1","url":null,"abstract":"<p><strong>Purpose: </strong>This study was aimed at comprehensive genomic analysis of VIM-type carbapenemase-producing Klebsiella pneumoniae species complex (KpSC) in Poland.</p><p><strong>Methods: </strong>All non-duplicate 214 VIM-producing KpSC isolates reported in Poland in 2006-2019 were short-read sequenced and re-identified by the average nucleotide identity scoring. Their clonality/phylogeny was assessed by cgMLST and SNP in comparison with genomes from international databases. Serotypes, VIM-encoding integrons, resistomes, virulomes and plasmid replicons were identified by various bioinformatic tools. Structures of plasmids and genomic islands with VIM integrons were analysed for representative long-read sequenced isolates.</p><p><strong>Results: </strong>The KpSC isolates were the second most prevalent VIM-positive Enterobacterales (23.1%) in Poland in 2006-2019, following Enterobacter spp. (40.1%). Their significance emerged in 2014 and then grew consequently, owing to eight regional outbreaks of K. pneumoniae sequence types (STs) ST437, ST147, ST15, ST277 and ST392. These carried different VIM integrons, mainly In238 and In916 types, located on IncFIB + IncHI2 (pNDM-MAR)-, IncA- or IncM-like plasmids, or clc-type integrative and conjugative elements. Despite relatedness of the outbreak clusters to isolates from other countries, e.g. Greece, Spain, Slovakia or Germany, most of them have apparently emerged on site by horizontal acquisition of resistance determinants from other species, including Enterobacter spp. and Pseudomonas spp.</p><p><strong>Conclusions: </strong>This work shows dynamic epidemiology of VIM-producing organisms, driven by a mix of circulation of different VIM-encoding elements, and parallel clonal spread of multiple organisms.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"619-628"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic accuracy of Xpert MTB/RIF Ultra for detecting pulmonary tuberculosis and rifampicin resistance: a systematic review and meta-analysis.","authors":"Man-Qing Wang, Ya-Fang Zheng, Yu-Qi Hu, Jin-Xia Huang, Zi-Xin Yuan, Zu-Yan Wu, Lu-Fang Huang, Chu-Ting Tang, Feng-Yi Zhang, Yan Chen, Jin-Ke He, Xu-Guang Guo, Bao-Mei Yan","doi":"10.1007/s10096-024-05032-1","DOIUrl":"10.1007/s10096-024-05032-1","url":null,"abstract":"<p><strong>Background: </strong>Public health issues related to tuberculosis still exist. Because Xpert MTB/RIF Ultra is more effective than conventional TB diagnostic techniques are, it is now regarded as an emerging technology. The diagnostic accuracy of Xpert MTB/RIF Ultra for tuberculosis was assessed in this systematic study.</p><p><strong>Methods: </strong>We searched the PubMed, Cochrane, EMBASE, and Web of Science databases for pertinent literature published before January 18, 2024. The quality of the collected literature was assessed via Review Manager 5.3 software, which applies the quality assessment of diagnostic accuracy studies criteria. Using Metadisc 1.40 software, the sensitivity, specificity, and summary receiver operating characteristic curves were plotted and examined. Stata 12.0 was the program we utilized to assess publication bias in this investigation. The Prospero prospective register of systematic reviews included this study (reference number CRD42024569674).</p><p><strong>Results: </strong>Analysis of 187 fourfold tables from 72 studies revealed that Xpert MTB/RIF Ultra demonstrated an overall pooled sensitivity of 76% and specificity of 95% for detecting pulmonary tuberculosis. The positive likelihood ratio (PLR) was 14.91, and the negative likelihood ratio (NLR) was 0.23, with an area under the curve (AUC) of 0.9351 and a diagnostic odds ratio (DOR) of 73.39. For detecting rifampin resistance, the combined sensitivity and specificity were 94% and 97%, respectively. The pooled PLR was 24.94, the NLR was 0.07, and the DOR was 429.05. The area under the summary receiver operating characteristic (SROC) curve was 0.9868.</p><p><strong>Conclusion: </strong>In conclusion, developing effective laboratory diagnostic tools for identifying Mycobacterium tuberculosis is essential for epidemiological research. This study demonstrated that Xpert MTB/RIF Ultra effectively diagnosed Mycobacterium tuberculosis infection, including pulmonary tuberculosis, as well as rifampin resistance, highlighting its potential as a valuable tool for both diagnosis and resistance detection.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"681-702"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142926842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of whole genome sequencing typing tools for the typing of Belgian Legionella pneumophila outbreaks isolates.","authors":"Fedoua Echahidi, Subin Park, Alaeddine Meghraoui, Florence Crombé, Oriane Soetens, Denis Piérard, Benoit Prevost, Ingrid Wybo, Charlotte Michel","doi":"10.1007/s10096-024-05013-4","DOIUrl":"10.1007/s10096-024-05013-4","url":null,"abstract":"<p><p>Whole genome sequencing (WGS) marks a turning point for outbreak investigations for microorganisms related to public health matters, like Legionella pneumophila (Lp). Here, we evaluated the available Lp WGS typing tools for isolates of previously documented Belgian outbreaks, as well as small groups of related and non-related isolates. One reference strain and 77 clinical and environmental isolates were evaluated. Seven isolates belong to a Sequence Type (ST) 36 outbreak in 1999 and sixteen (ten clinical, two matching environmental and four non-related controls) belong to another ST1 outbreak in 1985-1987. The remaining isolates belong to small groups of related and non-related isolates of diverse ST's. WGS was performed and data were analysed using whole genome (wg) and core genome (cg) multilocus sequence typing (MLST) with \"Ridom SeqSphere + \" (cgMLST), \"Applied Maths-Bionumerics\" (wgMLST) and the 50 loci cgMLST (CDC/ESGLI_ESCMID). Results of the three tools were concordant with the traditional Sequence Based Typing (SBT). The known outbreaks and small clusters could be detected and clear discrimination of ST1 non-related isolates was obtained. In addition, the 50 loci cgMLST allowed to classify the isolates into subtypes because almost all the 50 genes could be called in all the analysed isolates, which was not achieved by the other tools. This is a big advantage in terms of standardisation and comparison between laboratories for future epidemiological investigations. WGS allowed to analyse a large volume of samples and generated more accurate conclusions for outbreak investigations compared to other typing methods due to its higher discriminatory power and throughput.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"597-607"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11880184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acne vulgaris: advances in pathogenesis and prevention strategies.","authors":"Weiping Xu, Jiahui Xu, Dandan Huang, Chen Wang, Jiajia Song, Xiaoyong Chen, Huayi Suo","doi":"10.1007/s10096-024-04984-8","DOIUrl":"10.1007/s10096-024-04984-8","url":null,"abstract":"<p><strong>Purpose: </strong>The aim is to encourage the creation of innovative prevention and treatment measures and to help readers in selecting the most effective ones.</p><p><strong>Background: </strong>Acne vulgaris is the most prevalent skin condition of adolescents, affecting approximately 9% of the global population. Patients become more prone to mental and psychological problems because of it. Several strategies have been established to effectively improve acne vulgaris. However, the complexity of its pathogenesis and the limitations of the existing strategies to control it in terms of bacterial resistance, patient compliance, and safety have made the development of new control strategies a hot topic in skin health research.</p><p><strong>Results: </strong>This review systematically summarizes the pathogenesis and prevention strategies of acne vulgaris according to the most recent studies. The limitations of the current research on acne vulgaris and future research directions are presented based on the analysis of the strengths and weaknesses of the existing prevention and treatment strategies.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"515-532"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143002167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Neisseria meningitidis carriage among men who have sex with men at a sexual health clinic in Brussels, Belgium: a cross-sectional study.","authors":"Alice Doubinsky, Véronique Yvette Miendje Deyi, Wesley Mattheus, Marc Delforge, Agnès Libois, Nicolas Dauby, Rakan Nasreddine","doi":"10.1007/s10096-024-05031-2","DOIUrl":"10.1007/s10096-024-05031-2","url":null,"abstract":"<p><p>To assess the prevalence of Neisseria meningitidis (Nm) carriage among men who have sex with men (MSM) and examine potential risk factors associated with colonization. This was an observational, cross-sectional, monocentric study. Inclusion criteria were asymptomatic adult MSM. Recruitment took place at an outpatient sexual health clinic in Brussels, Belgium from October 2019 to March 2020. The primary outcome of this study was to determine the prevalence of meningococcal oropharyngeal carriage. Secondary outcomes included characterization of the participants colonized with Nm and serogroup identification of encapsulated strains. Ordinal logistic regression analysis was performed to evaluate for associations with Nm colonization. A total of 143 participants were included, of which 36 (25.2%) were found to be colonized with Nm. The most common encapsulated strain identified was serogroup B (50%). Colonized individuals had a median (IQR) age of 36.5 (29-43) years, 69.4% were Caucasian, and 38.9% indicated having 2 to 5 kissing-only partners while 47.2% reported having no sex partners during that same period. Multivariable analysis showed that not having been vaccinated against Nm in the past 5 years was the only characteristic significantly associated with being colonized with Nm (odds ratio 3.38; 95% confidence interval 1.26-6.58, p = 0.02). In this analysis of MSM, asymptomatic Nm carriage was observed to be 25.2% and serogroup B (50%) was the most frequently identified. Non-vaccination against Nm was the only factor significantly associated with colonization. Further research is needed to better understand Nm colonization in this population.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"739-745"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of OXA-23-producing Proteus mirabilis in Switzerland.","authors":"Jacqueline Findlay, Patrice Nordmann, Roger Stephan, Laurent Poirel","doi":"10.1007/s10096-024-04972-y","DOIUrl":"10.1007/s10096-024-04972-y","url":null,"abstract":"<p><p>Proteus mirabilis is a Gram-negative bacterium found in the environment and also forms part of the commensal flora in the gastrointestinal tract of both humans and animals. P. mirabilis can cause a wide variety of infections, however it does not harbor any intrinsic β-lactamase genes and as such usually exhibits full susceptibility to β-lactams with the exception of imipenem, to which it is naturally resistant. OXA-23 enzymes are carbapenem-hydrolyzing class D β-lactamases and are usually the main cause of acquired resistance to carbapenems in Acinetobacter baumannii but have recently been reported in P. mirabilis in France. In this report we describe the emergence of OXA-23-producing P. mirabilis clinical isolates from Switzerland.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"759-762"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142871753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of six different methods used in detection of colistin resistance in extensively drug-resistant K. pneumoniae, A. baumannii and P. aeruginosa isolates with the reference method broth microdilution method.","authors":"Özlem Aydemir, Sema Çetin, Nilay Can, Gökçen Ormanoğlu, Mehmet Köroğlu","doi":"10.1007/s10096-024-05019-y","DOIUrl":"10.1007/s10096-024-05019-y","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the agreement of different methods with the reference method in the detection of colistin resistance in extensively drug-resistant K. pneumoniae, A. baumannii and P. aeruginosa isolates.</p><p><strong>Materials and methods: </strong>K. pneumoniae, A. baumannii and P. aeruginosa isolates were included in the study. All isolates were subjected to colistin susceptibility testing with seven different methods. All results were compared with broth microdilution results. Categorical agreement (CA), very majorl error (VME) and major error (ME) rates were calculated for the tested methods.</p><p><strong>Results: </strong>In all methods, VME and ME rates were found to be above the acceptable performance limit for all strains. Although VME rates were above the target value in all strains, the lowest VME rates were detected in MRRP-NP with 12.5%. Among all isolates, the lowest ME rate was obtained in MCLDE with 10% and the highest ME rate was detected in VITEK 2^® with 19.9%. The highest ME rate was detected in VITEK 2^® with 27.3%. The highest CA in A. baumannii was detected in MRRP-NP; in P. aeruginosa, it was detected in MCLDE, MCLDER and MRRP-NP. CA in K. pneumoniae isolates was below the acceptable performance in all methods.</p><p><strong>Conclusions: </strong>The results obtained in this study show that the methods used in colistin susceptibility testing still need standardization. There is an urgent need for a colistin susceptibility testing method that is easy to apply, inexpensive and suitable for routine use.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"629-638"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis and virulence of human Streptococcus suis serotype 14.","authors":"Parichart Boueroy, Jaime Brizuela, Thomas J Roodsant, Thidathip Wongsurawat, Piroon Jenjaroenpun, Peechanika Chopjitt, Rujirat Hatrongjit, Nattamol Phetburom, Sorujsiri Chareonsudjai, Thidarut Boonmars, Constance Schultsz, Anusak Kerdsin","doi":"10.1007/s10096-024-05029-w","DOIUrl":"10.1007/s10096-024-05029-w","url":null,"abstract":"<p><strong>Purpose: </strong>Streptococcus suis serotype 14 is the second most prevalent serotype being highly prevalent in Southeast Asia. This study aimed to characterize genetic background, population structure, virulent genes, antimicrobial-resistant genes, and virulence of human S. suis serotype 14.</p><p><strong>Methods: </strong>Genomes of 11 S. suis serotype 14 were sequenced by short- and long-read sequencing platforms. The genomes were analyzed for genetic relationship, virulence-associated genes, and antimicrobial-resistant genes. Antimicrobial susceptibility was conducted and the virulence was tested based on cell assay.</p><p><strong>Results: </strong>All isolates belonged to clonal complex (CC) 1, with nine sequence type (ST) 105 isolates and each isolate of ST1 and ST237. They were susceptible to penicillin, whereas tetracycline and macrolide were resistance due to tetO and ermB. Genomic analysis revealed that the serotype 14-ST105 isolates were closely related to zoonotic serotype 14-ST105 isolates from Vietnam and the serotype 1-ST105 Thai strain. The serotype 14-ST1 isolate was closely related to pig-diseased serotype 1-ST1 isolates from UK and USA, whereas the serotype 14-ST237 isolate was related to serotype 1-ST237 strains recovered from healthy pig from Thailand. Of 150 virulence-associated genes, 13 were absent from the serotype 14 isolates, including atl1, atlAss, hhly3, nisK, nisR, pnuC, salK, salR, sp1, srtG, virB4, virD4, and zmp. The virulence of strain 32481, a representative S. suis serotype 14-ST105 isolate showed reduced adhesion and invasion of two epithelial cell lines (A549 and HeLa) when compared to the serotype 2-ST1 strain P1/7, whereas apoptosis was similar.</p><p><strong>Conclusion: </strong>This study highlighted the pathogenic potential of virulent serotype 14-ST105 strains and the need for increased monitoring of S. suis serotypes other than for serotype 2.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":"639-651"},"PeriodicalIF":3.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the genetic context of the emerging OXA-484-producing carbapenem-resistant Escherichia coli from ST167 high-risk clone in the United Arab Emirates.","authors":"Farah Al-Marzooq, Akela Ghazawi, Mushal Allam, Timothy Collyns","doi":"10.1007/s10096-025-05082-z","DOIUrl":"https://doi.org/10.1007/s10096-025-05082-z","url":null,"abstract":"<p><strong>Purpose: </strong>The evolution of new carbapenemase variants is alarming. We aimed to investigate the genetic context and molecular epidemiology of the emerging OXA-484 carbapenemase in the United Arab Emirates, to decipher its resistance mechanisms and evolutionary relationships.</p><p><strong>Methods: </strong>Antimicrobial susceptibility testing was performed for an E. coli isolate recovered from an intrauterine contraceptive device of a patient returning to the UAE after a trip to Pakistan. Whole genome sequencing was used to characterize the genetic environment of bla_OXA-484, resistance and virulence determinants. Southern blotting was used to localize OXA-484 gene. Phylogenetic analysis established the sequence type (ST) and used to investigate relationships with global strains, and differences from other OXA-48-like types.</p><p><strong>Results: </strong>The strain demonstrated selective resistance against ertapenem while maintaining susceptibility to imipenem and meropenem. OXA-484 exhibited R214G substitution affecting the enzyme's activity and distinguishing it from closely related variants like OXA-181 (214R) and OXA-232 (214 S). bla_OXA-484 was located on two non-conjugative plasmids (∼ 65 and 100 kb) within a genetic environment containing multiple insertion sequences. It belonged to the high-risk clone ST167, recognized for its enhanced capacity to acquire and maintain resistance determinants. The gene was mostly prevalent in the Western part of the world with limited distribution in the Middle East.</p><p><strong>Conclusion: </strong>This study presents the first comprehensive characterization of OXA-484-producing E. coli ST167 in the UAE. The presence of bla_OXA-484 in high-risk clone warrants concern on its dissemination potential and underscores the importance of genomic surveillance and targeted infection control to prevent the spread of emerging resistance determinants.</p>","PeriodicalId":11782,"journal":{"name":"European Journal of Clinical Microbiology & Infectious Diseases","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}