Entomological Research最新文献

{"title":"Pig manure characteristics and decomposition ability of Protaetia brevitarsis seulensis larval instar stages","authors":"Yeon-Woo Jeong","doi":"10.1111/1748-5967.12766","DOIUrl":"https://doi.org/10.1111/1748-5967.12766","url":null,"abstract":"<p>Many types of livestock waste can be treated with insect larvae; however, in-depth research on the decomposition ability of <i>Protaetia brevitarsis seulensis</i> (PBS) larvae and the characteristics of manure after treatment with PBS is extremely limited. Therefore, this study examined the decomposition ability and survival rates of different instars of PBS larvae and their effects on the characteristics and pathogen counts of pig manure. Two treatments were designed: 1 kg of second instar or third instar PBS larvae per 10 kg of pig manure. Pig manure did not affect the decomposing capacity and survival rates of the two PBS larval instar groups (<i>P</i> &gt; 0.05). However, the decomposition abilities of the different PBS larval instar groups on days 2, 7 and 8 significantly differed (<i>P</i> &lt; 0.05). Over time, the changes observed in pH, moisture, total nitrogen, acid detergent fiber (ADF), neutral detergent fiber (NDF) and hemicelluloses did not significantly differ (<i>P</i> &gt; 0.05) between treatments using second or third instar PBS larvae. On day 15, both total nitrogen and NDF significantly differed (<i>P</i> &lt; 0.05) between the two treatment groups. During the experimental period, no significant (<i>P</i> &gt; 0.05) change in <i>Escherichia coli</i> or <i>Salmonella</i> counts was observed in pig manure treated with different PBS larval instars. Our findings suggest that the decomposition abilities and survival rates of larvae and pig manure characteristics and pathogen counts do not differ for different PBS larval instar stages.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 11","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology and distribution of antennal sensilla of Plautia stali (Hemiptera: Pentatomidae)","authors":"Gwang Hyun Roh,&nbsp;Ji Hye Oh,&nbsp;Yeon Jeong Lee,&nbsp;Min-Jung Huh,&nbsp;Il-Kwon Park,&nbsp;Chung Gyoo Park","doi":"10.1111/1748-5967.12770","DOIUrl":"https://doi.org/10.1111/1748-5967.12770","url":null,"abstract":"<p>In this study, the morphology and distribution of antennal sensilla of male and female adults of <i>Plautia stali</i> were investigated using scanning electron microscopy. In both sexes, the antennae of <i>P. stali</i> were composed of a scape, two-segmented pedicels (P1 and P2) and two-segmented flagellomeres (F1 and F2). There were no differences in the overall length and shape between males and females. Four basic types of sensilla (s. trichodea, s. basiconica, s. chaetica and s. coeloconica) were identified in each sex. Sensilla basiconica and s. chaetica were further divided into four and two subtypes, respectively. Among the sensilla, s. trichodea and s. basiconica subtype 4 were multiporous, indicating that these two types are involved in olfactory functions. There was an apical pore on the tip of s. basiconica subtypes 2 and 3, suggesting that they have contact chemosensory function. There was no pore on the cuticular surface of the other types of sensilla, suggesting that these types may be mechano-, thermo- or hygroreceptors. Sexual dimorphism was not evident in the distribution of the four types of sensilla, although there were variations in the number and distribution of sensilla along with the antennae in both sexes. This study reveals information regarding the sensory function of each type of sensillum in <i>P. stali</i>.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 10","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.12770","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of the main components of garlic, Allium sativum, volatiles and their determination of repellent or toxic activities on green peach aphid, Myzus persicae","authors":"Rongquan Lai,&nbsp;Yuee Mi,&nbsp;Yufei Lai,&nbsp;Gang Gu,&nbsp;Zhihou Cheng,&nbsp;Chen Yang,&nbsp;Ting Zhou,&nbsp;Bang Zhang","doi":"10.1111/1748-5967.12769","DOIUrl":"https://doi.org/10.1111/1748-5967.12769","url":null,"abstract":"<p>To determine the control mechanism of garlic on <i>Myzus persicae</i>, volatiles from garlic plants were collected, and the components were determined by GC–MS. Then, the volatile extracts and standard components were screened by electroantennogram (EAG) apparatus and Y-tube olfactometer, and the toxic effects were determined by the immersion method. The results showed the main components from garlic plant volatile were 2-methoxyphenol, 2,4-di-tert-butylphenol, diallyl disulfide, butyl phthalate esters, (1-hydroxycyclohexyl) phenyl ketone, and 2,6-di-tert-butylp-cresol. The volatile extracts and the standard components had significant repellent effects (<i>P</i> ≤ 0.05) on <i>M. persicae</i> when the volume ratio was 1:100. The relative values of EAG from volatile extracts and diallyl disulfide were significantly higher than those of the other substances (<i>P</i> ≤ 0.05) when the volume ratio was 1:100. The toxicity of garlic volatile extracts to <i>M. persicae</i> significantly (<i>P</i> ≤ 0.05) increased with increasing concentration. After 72 h (treated), the adjusted mortality of the extract to <i>M. persicae</i> was 36.99% at 90 ml/L. The standard components had the significant toxic effect on <i>M. persicae</i> at 30 mg/mL, and the rates of mortality were over 78.95%. Thus, the results suggested that the volatile extracts and the four standard components from garlic plants had significant repellent effects and electroantennogram responses for <i>M. persicae</i>, and the toxic effects were stronger than the repellent effects for <i>M. persicae</i>.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 10","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut bacteria-derived volatiles mediate Drosophila melanogaster attraction","authors":"Xianwei Gou,&nbsp;Yilu Feng,&nbsp;Ge Yi,&nbsp;Nan Yang,&nbsp;Shu-Wen Zhang,&nbsp;Xing-Jiang Qi,&nbsp;Wenwu Zhou,&nbsp;Jianchu Mo,&nbsp;Rong Xu,&nbsp;Hongjie Li","doi":"10.1111/1748-5967.12768","DOIUrl":"https://doi.org/10.1111/1748-5967.12768","url":null,"abstract":"<p>Bacteria-originated volatile molecules play a crucial role in chemical communications between insects, representing their promising application as odor bait traps in pest control. In this study, we investigated the behavioral preferences of the fruit fly <i>Drosophila melanogaster</i> (Diptera: Drosophilidae) towards the fermentation broth of seven gut-associated bacteria using trap choice assays. All seven bacterial fermentations significantly attracted adults and larvae compared to the medium control. We assessed the effects of bacterial fermentations on bayberry attractiveness to fly adults, and found that the bayberries sprayed with fermentation broth of seven bacteria were all significantly more attractive to adults than the non-sprayed, following in increased total egg numbers. We also compared the attractive effect of bacterial fermentations with a sugar-vinegar mixture and a commercial odor-bait. The commercial odor bait proved more enticing than the unconcentrated 5-day fermentation broths. However, out of the seven bacteria, 64-fold concentrated bacterial fermentation of <i>Corynebacterium</i> (Actinomycetota phylum) was significantly more attractive than commercial bait. Finally, we chemically identified the predominant compounds 2-methylpropanal and acetaldehyde, which are likely responsible for the behavioral preference of fruit flies. Our findings provide a deeper understanding of how gut microbes affect insect behavior and offer a potential bacteria-originated odor bait for fly control in the orchard.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 10","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142429365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition and mosquitocidal potential of Gloriosa superba (flame lily) seed extract against the dengue mosquito Aedes aegypti and aquatic mosquito predators","authors":"Venkatachalam Ramasamy,&nbsp;Palanisamy Prakash,&nbsp;Prabhakaran Vasantha-Srinivasan,&nbsp;Yeon Soo Han,&nbsp;Sengodan Karthi,&nbsp;Sengottayan Senthil-Nathan,&nbsp;Ekambaram Gayathiri,&nbsp;Ki Beom Park,&nbsp;Subramaniam Umavathi","doi":"10.1111/1748-5967.12767","DOIUrl":"https://doi.org/10.1111/1748-5967.12767","url":null,"abstract":"<p>This study investigates the mosquitocidal potential of crude petroleum ether extract fractions derived from <i>Gloriosa superba</i> against the dengue mosquito vector <i>Aedes aegypti</i>. Chemical profiling through gas chromatography identified four major metabolites, with nonadecane showing the highest peak area percentage (70.34%). A lethal dosage of fraction IV of <i>G. superba</i> (Gs-F4), at a maximum concentration of 5.0 ppm, exhibited high mortality rates in second (78%), third (71%) and fourth (51%) instars of <i>Ae. aegypti</i>. A sublethal dosage of Gs-F4 (3.0 ppm) significantly altered the larval midgut enzymes of cytochrome P450, esterases and glutathione <i>S</i>-transferases. The repellent activity of Gs-F4 was dosage dependent, with repellency percentages decreasing from 96% to 50% as the exposure time increased from 15 to 210 mins. The impact of Gs-F4 on aquatic mosquito predators was marginally toxic (&lt;50%) compared with the commercial compound temephos (1.0 ppm), which exhibited a higher toxicity (&gt;50%). <i>In silico</i> toxicity screening using the BeeToxAI server indicated that three of the four compounds in Gs-F4 were safe or non-toxic to honeybees, except for <i>α</i>-gurjunene (6.29 <i>μ</i>g/bee). Although <i>G</i><i>. superba</i> demonstrates effective larvicidal and repellent activity against <i>Ae. aegypti</i>, the non-target toxicity against mosquito predators necessitates further toxicological screening on beneficial species under field conditions.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 9","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.12767","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization and immune association of Fas-associated death domain protein (FADD) in Tenebrio molitor","authors":"Keunho Yun,&nbsp;Ho Am Jang,&nbsp;Bharat Bhusan Patnaik,&nbsp;Su Hyeon Hwang,&nbsp;Yong Seok Lee,&nbsp;Yeon Soo Han,&nbsp;Yong Hun Jo","doi":"10.1111/1748-5967.12765","DOIUrl":"https://doi.org/10.1111/1748-5967.12765","url":null,"abstract":"<p>Fas-associated death domain protein (FADD) is an apoptotic adapter molecule that recruits caspases and facilitates the formation of death-inducing signaling complexes in mammals. In lower animals, FADD forms a complex with immune deficiency and death-related ced-3/Nedd2-like caspase to elicit antimicrobial responses. In this study, we characterized the FADD sequence of <i>Tenebrio molitor</i> (TmFADD) using molecular informatics to understand its role in immune surveillance of the host against microorganisms. The 618 bp open reading frame of <i>TmFADD</i> encodes a polypeptide of 204 amino acids. The protein includes two promiscuous domains, the death-effector domain and the death domain, found at the N- and C-termini, respectively. TmFADD is clustered with the coleopteran FADD sequences in the phylogenetic tree. Homology-based structural analysis showed an <i>α</i>-helical model with a QMEAN score of 0.58. The expression of <i>TmFADD</i> mRNA was conspicuous in the larval and early pupal stages. <i>TmFADD</i> mRNA was distributed in the immune tissues of the larva and was found predominately in the gut of the adult. Infection of the host with <i>Candida albicans</i>, <i>Escherichia coli</i> or <i>Staphylococcus aureus</i> increased the expression of <i>TmFADD</i> mRNA, suggesting that these microorganisms trigger the innate immunity of the host. The silencing of <i>TmFADD</i> transcripts resulted in an increased susceptibility of <i>T. molitor</i> larvae to infection with <i>E. coli</i>, but not to infection with <i>C. albicans</i> or <i>S. aureus</i>. Hence, these results indicate that FADD plays a role in the defense of <i>T. molitor</i> and could open avenues for understanding insect immunity.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 9","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.12765","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142316749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of weir-induced habitat fragmentation on the diversity and community composition of benthic macroinvertebrates in an agricultural stream","authors":"Sung-Ho Lim,&nbsp;Yuno Do","doi":"10.1111/1748-5967.12764","DOIUrl":"https://doi.org/10.1111/1748-5967.12764","url":null,"abstract":"<p>This research delves into the effects of weir-induced habitat fragmentation on the diversity and structure of benthic macroinvertebrate communities in Topyeong Stream in the Republic of Korea. Through comprehensive zonation, environmental assessments and analyses of macroinvertebrate populations, the study brings to light the significant impact of physical barriers within aquatic ecosystems. Findings reveal notable differences in macroinvertebrate diversity among various stream zones, with alpha diversity measurements demonstrating the significant influence of habitat conditions on biodiversity. Beta diversity analyses uncovered considerable species turnover, pointing to distinct ecological features within fragmented habitats. Mantel tests solidified a significant link between geographical separation and species composition, highlighting the critical impact of spatial isolation arising from habitat fragmentation. The research also pinpointed environmental factors, including habitat size and depth variation, as critical influences on macroinvertebrate community dynamics. Discussions on conservation tactics centered on habitat restoration and forward-thinking water management strategies aim to counteract the negative consequences of fragmentation and drought. This study emphasizes the essential need for cohesive conservation initiatives to preserve aquatic biodiversity and the functionality of ecosystems amidst human-induced challenges.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 9","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.12764","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142233142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of Anaplasma phagocytophilum, Borrelia theileri and Severe fever with thrombocytopenia syndrome virus (SFTSV) in military working dogs and ticks collected from the Republic of Korea Army garrisons in Gangwon Province in 2021–2022","authors":"Minsung Kim,&nbsp;Sunghoon Cho,&nbsp;Gyeonggook Park,&nbsp;Jeongyun Kim,&nbsp;Misun Rieu,&nbsp;Kyung Tae Noh,&nbsp;Sangyun Ha,&nbsp;Quehn Park,&nbsp;Du Hwan Kim,&nbsp;Sangbeom Han,&nbsp;Geontae Jeon,&nbsp;Min Seong Park,&nbsp;Buddle Lee,&nbsp;Beonmman Ha,&nbsp;Ki Beom Park,&nbsp;Prabhakaran Vasantha-Srinivasan,&nbsp;Yeon Soo Han,&nbsp;Chanhee Lee","doi":"10.1111/1748-5967.12762","DOIUrl":"https://doi.org/10.1111/1748-5967.12762","url":null,"abstract":"<p>Tick-borne pathogens (TBPs) are an emerging concern to humans and animals in the Republic of Korea, especially within the military zones of Gangwon Province, a mountainous region abundant with wild fauna and ticks. This study aimed to detect six TBPs in military working dogs (MWDs) and ticks in this region using Nested polymerase chain reaction (nested PCR) and sequencing. The targeted pathogens included <i>Anaplasma phagocytophilum</i>, <i>Bartonella henselae</i>, <i>Borrelia</i> spp., <i>Orientia tsutsugamushi</i>, Severe fever with thrombocytopenia syndrome virus (SFTSV) and tick-borne encephalitis virus (TBEV). A total of 164 serum samples from MWDs were collected at the Korean Army Military working dog training center and 1418 ticks were collected at various training sites between June 2021 and August 2022. <i>Haemaphysalis longicornis</i> was identified as the predominant species (98.3%, <i>n</i> = 1394), followed by <i>Haemaphysalis flava</i> (1.6%, <i>n</i> = 22) and <i>Ixodes nipponensis</i> (0.1%, <i>n</i> = 2). This study detected <i>A. phagocytophilum</i> and <i>Borrelia theileri</i> in 1.4% (1/72) of the tick pools and detected SFTSV in 0.6% (1/164) of MWD serum samples. The MWD found to be infected with SFTS was a German Shepherd, but showed no significant clinical signs, with a normal complete blood count (CBC). In the phylogenetic analysis, three sequences were acquired. One sequence (OR865211) exhibited 100% homology with <i>16S</i> rRNA of <i>A. phagocytophilum</i> identified from animals and human patients in the Republic of Korea. Another sequence (OR865152) displayed 99.34%–100% sequence similarity with <i>16S rRNA</i> of <i>B. theileri</i> fragments. The third sequence (OR865115), which belongs to the SFTS B-1 genotype, showed 94.7% sequence similarity to a strain identified in the Republic of Korea (KY789441).</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 8","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142077978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of climatic and time-related variables on dung beetle communities: A case study in Central Spain","authors":"Sandra Grzechnik,&nbsp;Francisco José Cabrero-Sañudo","doi":"10.1111/1748-5967.12763","DOIUrl":"https://doi.org/10.1111/1748-5967.12763","url":null,"abstract":"<p>Dung beetles are considered a key element in ecosystems as they are involved in many ecological processes, being one of the main decomposers of organic matter in the landscape. They can be classified into 3 subfamilies: Aphodiinae, Geotrupinae and Scarabaeinae, with each subfamily exhibiting specific adaptations and evolutionary strategies that have developed over time. The global patterns of dung beetle diversity are influenced by ecological factors, such as climatic (temperature, humidity, atmospheric pressure) and time-related variables. Thus, the aim of the study is to see how these variables affect a dung beetle community and whether there are different responses among the subfamilies. The study was carried out in Mataelpino, a town located in Central Spain (Madrid, Spain). Monthly sampling was conducted from May 2018 to February 2020. According to the results the variables considered exert an effect on dung beetles, with differences observed among the subfamilies. To our knowledge, atmospheric pressure has been considered for the first time in a study of this type, with a greater effect being observed in the Aphodiinae subfamily than in the rest of the dung beetle groups. Regarding the other subfamilies, it has been observed that temperature is a determining factor for Scarabaeinae species, whereas humidity seems to have a greater effect on Geotrupinae species.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 8","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.12763","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142077976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression, purification, and function of baculovirus infected insect cells-derived hookworm AIP-1 and AIP-2 proteins","authors":"Chae Eun Lee,&nbsp;Hyun Joo Chung,&nbsp;Da Won Lee,&nbsp;Jong Seok Lim,&nbsp;Jeong Hwan Lee,&nbsp;Kisung Ko,&nbsp;Soon Auck Hong,&nbsp;Min Eui Hong,&nbsp;Joo Young Kim,&nbsp;Hye Jun Lee,&nbsp;Jin Wook Kim,&nbsp;Soon Chul Myung","doi":"10.1111/1748-5967.12760","DOIUrl":"https://doi.org/10.1111/1748-5967.12760","url":null,"abstract":"<p>Anti-inflammatory protein (AIP)-1 and AIP-2, identified as parasite excretory/secretory (ES) proteins, play a crucial role in promoting the survival of the parasite and evading the host immunological response. Both proteins inhibit inflammatory reactions, induce apoptosis in effector cells, and influence the phenotype of the immune response. Numerous parasite-derived proteins have shown promise as therapeutic targets for inflammatory and allergic diseases. Despite this, the precise biological roles, and molecular characteristics of many such proteins remain unclear.</p><p>In this study, AIP-1 and AIP-2 were produced in the baculovirus-insect cell expression system (BEVS). The multiplicity of infection (MOI) and the duration of infection conditions for AIP-1 and AIP-2 protein expression were successfully optimized to induce the highest expression levels of AIP-1 and AIP-2 proteins in insect cells. The insect cell-derived AIP-1 and AIP-2 exhibited inhibitory functions against human matrix metalloproteinases (MMPs). This study demonstrates that insect cell-derived AIP-1 and AIP-2 have the potential as therapeutic proteins for MMP-TIMP (Metalloprotease-Tissue inhibitor of metalloprotease) axis related disease.</p>","PeriodicalId":11776,"journal":{"name":"Entomological Research","volume":"54 8","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1748-5967.12760","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141973673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}