Environmental Entomology最新文献_第2页

Temperature fluctuation effects on the demography and fitness of Xyleborus bispinatus (Coleoptera: Curculionidae: Scolytinae): thresholds and growth rates. 温度波动对双叶木蚁种群分布和适宜度的影响：阈值和生长率。

IF 1.8 3区农林科学

Environmental Entomology Pub Date : 2025-06-26 DOI: 10.1093/ee/nvaf061

Lucas A Fadda, Luis A Ibarra-Juárez, Luis Osorio-Olvera, Jorge Soberón, Andrés Lira-Noriega

{"title":"Temperature fluctuation effects on the demography and fitness of Xyleborus bispinatus (Coleoptera: Curculionidae: Scolytinae): thresholds and growth rates.","authors":"Lucas A Fadda, Luis A Ibarra-Juárez, Luis Osorio-Olvera, Jorge Soberón, Andrés Lira-Noriega","doi":"10.1093/ee/nvaf061","DOIUrl":"https://doi.org/10.1093/ee/nvaf061","url":null,"abstract":"Understanding the demography of Xyleborini ambrosia beetles and accurately estimating their optimal growth temperatures remains a challenge due to their cryptic behavior and complex reproductive habits. In this study, we reared the ambrosia beetle Xyleborus bispinatus at five distinct temperatures (17 °C, 20 °C, 26 °C, 29 °C, and 35 °C) over a 36-d period. Population dynamics, growth rates, and life cycle durations were assessed through destructive sampling every 4 d for each temperature treatment. To analyze temperature-dependent growth and development, a nonlinear model was fitted to the intrinsic growth rate values at each temperature, allowing us to determine the species' optimal temperature and corresponding maximum growth rate. In the laboratory, X. bispinatus exhibited optimal growth rates between 26 °C and 29 °C, with rates of 0.10 and 0.12, individuals/individuals/day, and life cycle durations of 20 and 16 d, respectively. However, based on the fitted growth curve, the thermal optimum was estimated at 26.2 °C, where the maximum intrinsic growth rate reached 0.13 individuals/individuals/day. Given its potential to transmit Harringtonia lauricola, these findings provide valuable insights into the thermal effects on X. bispinatus throughout its life cycle and offer a practical approach for estimating growth rates and thresholds in species with cryptic behavior or where cohort tracking is challenging for demographic assessments.","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cold tolerance strategy and lower temperature thresholds of Lycorma delicatula (Hemiptera: Fulgoridae) egg masses. 细蛾虫卵群的耐寒策略及低温阈值。

IF 1.8 3区农林科学

Environmental Entomology Pub Date : 2025-06-21 DOI: 10.1093/ee/nvaf007

Anna J Turbelin, Brent J Sinclair, John Rost, Amanda D Roe

{"title":"Cold tolerance strategy and lower temperature thresholds of Lycorma delicatula (Hemiptera: Fulgoridae) egg masses.","authors":"Anna J Turbelin, Brent J Sinclair, John Rost, Amanda D Roe","doi":"10.1093/ee/nvaf007","DOIUrl":"https://doi.org/10.1093/ee/nvaf007","url":null,"abstract":"The spotted lanternfly (Lycorma delicatula, Hemiptera: Fulgoridae) is a planthopper native to China and southeastern Asia invasive in North America. To inform the risk assessment of northward spread, we determined the cold tolerance strategy and lower lethal limits of overwintering eggs. We used field-collected egg masses from Pennsylvania, USA, and treated them with short (1 or 12 h) and long (10 or 15 d) low temperature exposures (0 °C to -25 °C). We determined that eggs were chill susceptible, and their supercooling points ranged from -17.4 °C to -27.7°C. We observed successful hatch after short- and long-term exposure to temperatures below -20 °C. Hatch rates were lower at or below -20 °C in the short exposures, or -15 °C in our long exposures experiments than hatch rate in egg masses not exposed to any treatment. Because L. delicatula eggs survived temperatures near their supercooling points, -27.7 °C could be used as an estimate of the lower lethal temperature. Our findings suggest that L. delicatula may be able to survive in colder climates than initially thought. Of the locations where L. delicatula has been intercepted in Canada-central and eastern provinces-Winnipeg is the only location where frequent or intense cold spells are likely to prevent hatch. Thus, low winter temperatures may not limit L. delicatula overwinter survival in many regions of Canada or other parts of the world that experience similar winter conditions.","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dose-response to sex attractants, and flight phenology of click beetle species (Elateridae) native to eastern North America. 原产于北美东部的击甲对性引诱剂的剂量反应及飞行物候。

IF 1.8 3区农林科学

Environmental Entomology Pub Date : 2025-06-20 DOI: 10.1093/ee/nvaf066

Lawrence M Hanks, Elijah J Davis, Thomas C McElrath, Yunfan Zou, Jocelyn G Millar

{"title":"Dose-response to sex attractants, and flight phenology of click beetle species (Elateridae) native to eastern North America.","authors":"Lawrence M Hanks, Elijah J Davis, Thomas C McElrath, Yunfan Zou, Jocelyn G Millar","doi":"10.1093/ee/nvaf066","DOIUrl":"https://doi.org/10.1093/ee/nvaf066","url":null,"abstract":"Females of most species of click beetles (Coleoptera: Elateridae) emit sex pheromones that attract males, and these chemicals can be utilized in monitoring and managing pest species. Here, we conducted dose-response trials with sex attractants of 5 elaterid species native to Illinois. Synthesized attractants were dispensed from polyethylene sachets, with doses of 0 (solvent control), 1, 3.3, and 10 mg. For 2 Gambrinus species, only traps baited with 10 mg doses of limoniic acid captured significantly more beetles than did control traps. However, for another 3 elaterid species, traps baited with 3.3 and 10 mg of attractants captured more beetles than did control traps, including Elater abruptus Say (attractant 5-methylhexyl [Z]-4-decenoate), Melanotus ignobilis Melsheimer (11-dodecenyl butyrate), and Parallelostethus attenuatus (Say) (1,8-octanediol dihexanoate). A separate field experiment, that tested 2 types of dispensers loaded with attractants, showed that traps baited with polyethylene sachet dispensers captured significantly more males of E. abruptus and P. attenuatus than did traps baited with rubber septa. We also assessed seasonal and daily activity periods of E. abruptus, M. ignobilis, and P. attenuatus, using attractant-baited panel traps. Flight periods of all 3 species were generally unimodal and confined to the summer months. Diel activity periods, assessed with attractant-baited panel traps fitted with collection jars that rotated at intervals of 1 to 2 h, showed that males of E. abruptus and P. attenuatus were primarily diurnal, flying from early morning through the evening, whereas males of M. ignobilis were nocturnal.","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flight tube and field cage studies of harmonic radar tagged Bactrocera dorsalis and Ceratitis capitata (Diptera, Tephritidae): effects of tag size, sex, rearing, and irradiation. 谐波雷达标记的背小实蝇和头角certis（双翅目，绦虫科）的飞行管和野外笼研究：标记大小、性别、饲养和辐照的影响。

IF 1.8 3区农林科学

Environmental Entomology Pub Date : 2025-06-19 DOI: 10.1093/ee/nvaf064

Matthew S Siderhurst, Anika L Hurst, Nicolas Ladizinsky, Charles J Mason, Sayaka Aoki

{"title":"Flight tube and field cage studies of harmonic radar tagged Bactrocera dorsalis and Ceratitis capitata (Diptera, Tephritidae): effects of tag size, sex, rearing, and irradiation.","authors":"Matthew S Siderhurst, Anika L Hurst, Nicolas Ladizinsky, Charles J Mason, Sayaka Aoki","doi":"10.1093/ee/nvaf064","DOIUrl":"https://doi.org/10.1093/ee/nvaf064","url":null,"abstract":"Tracking individual insects with harmonic radar (HR) allows observations of insect movement behavior under wild and semi-wild conditions. However, attaching tags to insects has the potential to alter natural behavior. Quantifying the negative effects of tag attachment may allow researchers to correct movement parameters to account for deviations from natural movements, thus producing more accurate estimates of insect behavior. Using two HR tag sizes, ~350 (small) and ~570 μg (large), the movements of Ceratitis capitata and Bactrocera dorsalis were investigated in flight tubes and an outdoor field cage. Bactrocera dorsalis with small tags attached did not perform differently than untagged flies in flight tubes, while flies with large tags took longer to exit the tubes. Time to exit did not vary with B. dorsalis body size, wing area, or wing length for any tagged status (untagged, small, or large). However, B. dorsalis exit times did vary with rearing and irradiation status but not with sex. For C. capitata, negative impacts were observed on both the time to exit and the failure to exit rate. Field cage experiments revealed differences in movement parameters among tested groups. For instance, female, colony-reared, non-irradiated, B. dorsalis showed the greatest bias in (pseudo) turning angles; male, wild, non-irradiated, B. dorsalis showed the weakest correlation between flight and wind direction (β); and colony-reared C. capitata showed random movement directions (other groups showed directional biases). This study shows the feasibility of tracking tephritids as small as C. capitata and demonstrates the advantages of using the smallest possible tags.","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laboratory and field evaluation of integrated insecticide-fungicide treatments for controlling Euwallacea interjectus and its symbiotic Fusarium fungi. 杀虫剂-杀菌剂综合处理对菊苣及其共生真菌镰刀菌的室内和现场评价。

IF 1.8 3区农林科学

Environmental Entomology Pub Date : 2025-06-19 DOI: 10.1093/ee/nvaf054

Weiyi Pan, Mengyi Lu, Shengchang Lai, Xuenong Ding, Bei Sun, Dinggen Ji, Dejun Hao, Lulu Dai

{"title":"Laboratory and field evaluation of integrated insecticide-fungicide treatments for controlling Euwallacea interjectus and its symbiotic Fusarium fungi.","authors":"Weiyi Pan, Mengyi Lu, Shengchang Lai, Xuenong Ding, Bei Sun, Dinggen Ji, Dejun Hao, Lulu Dai","doi":"10.1093/ee/nvaf054","DOIUrl":"https://doi.org/10.1093/ee/nvaf054","url":null,"abstract":"As an emerging forestry pest characterized by rapid spread, wide distribution, and significant damage, Euwallacea interjectus necessitates comprehensive and scientifically validated chemical control measures. Currently, there is a lack of large-scale and precise experimental data on the efficacy of insecticides and fungicides for chemical control. Even for closely related ambrosia beetles, existing chemical control methods have unresolved aspects. Given the high dependency of E. interjectus life cycle on its symbiotic fungi, this study employed fungicides as a pivotal control strategy. Additionally, to mitigate potential secondary environmental hazards associated with broad-spectrum insecticides, detailed toxicity tests were conducted in laboratory settings, followed by field spray experiments. An organosilicon compound was utilized to examine the synergistic effects of insecticides, and liquid chromatography was employed to quantify insecticide residues in the xylem of host trees. In the laboratory, beta-cyfluthrin exhibited the lowest LC50 value (8.989 mg/L), achieving an 80.6% reduction in beetle infestations after 30 d. The addition of adjuvants increased residue levels by 2.5%, thereby enhancing control efficacy. Among the tested fungicides, a 4:1 mixture of fludioxonil and prochloraz-manganese chloride complex demonstrated the highest efficacy, with an EC50 value of 2.669 mg/L. In field experiments, spraying at a concentration of 2000 mg/L resulted in a 58.7% reduction in beetle infestations after 60 d. The findings provide a scientific basis for the control of E. interjectus and the judicious application of chemical pesticides, offering technical support for the management of wood-boring pests in forestry production.","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection and validation of reference genes for RT-qPCR analysis of the Longan psyllid Cornegenapsylla sinica (Hemiptera: Psyllidae). 龙眼木虱Cornegenapsylla sinica（半翅目：木虱科）RT-qPCR内参基因的选择与验证

IF 1.8 3区农林科学

Environmental Entomology Pub Date : 2025-06-19 DOI: 10.1093/ee/nvaf059

Jun-Hong Qiu, Si-Yu Wang, Rong-Yue Hu, Da Ou, Bao-Li Qiu

{"title":"Selection and validation of reference genes for RT-qPCR analysis of the Longan psyllid Cornegenapsylla sinica (Hemiptera: Psyllidae).","authors":"Jun-Hong Qiu, Si-Yu Wang, Rong-Yue Hu, Da Ou, Bao-Li Qiu","doi":"10.1093/ee/nvaf059","DOIUrl":"https://doi.org/10.1093/ee/nvaf059","url":null,"abstract":"Cornegenapsylla sinica is a devastating pest of longan that vectors the longan pathogen witches' broom virus (LgWB), leading to significant agricultural losses. Efficient control strategies targeting this pest are imperative for sustainable longan production. However, the genetic research on C. sinica is relatively limited, which may hinder the discovery of effective control strategies. Accurate gene expression analysis under various conditions using RT-qPCR is essential for advancing our understanding of this pest and for identifying potential targets for management. In this study, a comprehensive array of specific algorithms, including geNorm, Normfinder, BestKeeper, and the ΔCt method, was applied to assess the stability of 8 candidate reference genes under 4 distinct experimental conditions: developmental stages, sex, tissue, and temperature. Through the application of RefFinder software, a ranking of expression stability among the candidate genes was established. The results indicated that RPL13 and RPL6 were the most stable reference genes under varying developmental stages and temperatures, ATPB and RPL13 were the top choices for different sexes, and RPL13 and EF1α were the most stable in different tissues. Additionally, heat shock protein 70 (Hsp70) served as a reporter gene to validate the selected reference genes. This study is the first to report detailed data on comprehensive reference genes suitable for RT-qPCR in C. sinica, laying the groundwork for biological control and functional target gene research in this species, which is crucial for preventing the spread of longan witches' broom virus in longan trees.","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concordance of eclosion life history timing across trophic levels in communities of host plants, fruit flies, and parasitoid wasps in the Pacific Northwest, USA. 美国太平洋西北地区寄主植物、果蝇和拟寄生蜂群落在营养水平上羽化生活史时间的一致性。

IF 1.8 3区农林科学

Environmental Entomology Pub Date : 2025-06-19 DOI: 10.1093/ee/nvaf050

Wee L Yee, Glen Ray Hood, Joshua M Milnes, Andrew A Forbes, Jeffrey L Feder

{"title":"Concordance of eclosion life history timing across trophic levels in communities of host plants, fruit flies, and parasitoid wasps in the Pacific Northwest, USA.","authors":"Wee L Yee, Glen Ray Hood, Joshua M Milnes, Andrew A Forbes, Jeffrey L Feder","doi":"10.1093/ee/nvaf050","DOIUrl":"https://doi.org/10.1093/ee/nvaf050","url":null,"abstract":"Whether host plant fruiting phenology variation affects eclosion times and generates allochronic isolation across trophic levels for Rhagoletis flies (Diptera: Tephritidae) and their braconid parasitoids is largely unknown, except in the Rhagoletis pomonella (Walsh) hawthorn-apple system. Here, we investigated how fly and wasp eclosion times in 4 systems-Oregon grape, Rhagoletis berberis Curran; red osier dogwood, Rhagoletis tabellaria (Fitch); bitter cherry, Rhagoletis indifferens Curran; and snowberry, Rhagoletis zephyria Snow-in Washington state, USA correspond to host phenology. Fruiting patterns differed among plants. Oregon grape and bitter cherry fruited once in relatively narrow temporal windows, while red osier dogwood and snowberry fruited multiple times or more widely through the season. Fruiting times differed for plants from earlier to later: based on fruit set, Oregon grape < red osier dogwood = bitter cherry < snowberry; first ripening fruit, Oregon grape < bitter cherry = snowberry; percent mature fruit in early August, Oregon grape = red osier dogwood < bitter cherry < snowberry. Mean fly eclosion times generally matched host fruiting times: red osier dogwood fly < Oregon grape fly = bitter cherry fly < snowberry fly. Parasitoid and fly eclosion matched except snowberry fly wasps eclosed before bitter cherry fly wasps. Differences in eclosion times between flies or wasps resulted in allochronic isolation from 1% to 96%. Nonconcordance between timing of fruit maturity and fly/wasp eclosion may be related to differences in precise host fruit or fly stages attacked by different species. Fruiting phenology may select for variable fly eclosion times, leading to a range of allochronic isolation between different Rhagoletis species and their associated guilds of parasitoid wasps.","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Entomopathogens for control of Asian longhorned beetles (Coleoptera: Cerambycidae). 防治亚洲长角甲虫（鞘翅目：天牛科）的昆虫病原体。

IF 1.8 3区农林科学

Environmental Entomology Pub Date : 2025-06-19 DOI: 10.1093/ee/nvaf016

Ann E Hajek, Eric H Clifton, Leellen F Solter

{"title":"Entomopathogens for control of Asian longhorned beetles (Coleoptera: Cerambycidae).","authors":"Ann E Hajek, Eric H Clifton, Leellen F Solter","doi":"10.1093/ee/nvaf016","DOIUrl":"https://doi.org/10.1093/ee/nvaf016","url":null,"abstract":"Asian longhorned beetles, Anoplophora glabripennis (Motschulsky) (ALB), are native to China and Korea but invasive populations now threaten hardwood forests in North America and Europe where eradication is the main tactic. In North America, invasive ALB were first found in the New York City area in 1996 and, since then, infestations were detected in 5 additional states. Beginning in 1998, studies of fungal entomopathogens for ALB control were begun, followed by studies with entomopathogenic nematodes, Bacillus thuringiensis Berliner, and microsporidia. Studies with entomopathogenic fungi initially focused on a commercialized method used for application of Beauveria asiatica against adult cerambycids in Japanese orchards: cerambycids walk across bands covered with infective spores of entomopathogenic fungi that are attached around trees. For use in the United States, Metarhizium brunneum Petch F52 was grown in fungal bands and tested against adult ALB in US quarantine labs as well as in the field in China. Fungal infection reduced female longevity, fitness, and flight. Long-lived M. brunneum microsclerotia formulated in hydromulch were investigated as an alternative application method. Several species of entomopathogenic nematodes applied to ALB emergence holes showed promise for infecting and killing ALB larvae. The pathogens that have been investigated and developed are considered part of the arsenal of methods for ALB control and not \"stand-alone\" tactics. While no pathogens have been used operationally in the United States, information gained about these pathogens is available and could be used as situations warrant more tools for managing ALB.","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetracycline changes the microbial assembly of Hyphantria cunea and reduces its fitness. 四环素改变了加利福尼亚杆菌的微生物组合，降低了它的适应性。

IF 1.8 3区农林科学

Environmental Entomology Pub Date : 2025-06-19 DOI: 10.1093/ee/nvaf063

Tong-Pu Li, Chen-Hao Wang, Zhi-Heng Wang, Bing-Ren Hao, Jia-Chu Xie, Hao-Xin Li, Dong-Jie Cai, Shu-Cheng Ye, Lv-Quan Zhao

{"title":"Tetracycline changes the microbial assembly of Hyphantria cunea and reduces its fitness.","authors":"Tong-Pu Li, Chen-Hao Wang, Zhi-Heng Wang, Bing-Ren Hao, Jia-Chu Xie, Hao-Xin Li, Dong-Jie Cai, Shu-Cheng Ye, Lv-Quan Zhao","doi":"10.1093/ee/nvaf063","DOIUrl":"https://doi.org/10.1093/ee/nvaf063","url":null,"abstract":"The strong environmental adaptability of the fall webworm Hyphantria cunea is closely linked to its internal microbiota. However, with the widespread usage and environmental residuals of antibiotics, their effects on the microbiome and fitness of H. cunea remain unknown. In this study, we found that tetracycline significantly decreased bacterial diversity in H. cunea, evident in a notable reduction in both the number of bacterial species and their relative abundance. Furthermore, the bacterial community structure was influenced by tetracycline, resulting in an increase in the relative abundance of Firmicutes and a decrease in the relative abundances of Proteobacteria, Bacteroidota, and others. Additionally, the potentially tetracycline-resistant bacterial genus ZOR0006 became dominant after tetracycline ingestion. The tetracycline also led to significant alterations in the function of the bacterial community, particularly in defense mechanisms, cell motility, energy production, and conversion. Corresponding to the changes in the microbiota, tetracycline significantly reduced the fitness of H. cunea, mainly manifested as an extension of the developmental duration and a decrease in the survival rate. These findings elucidate the effects of tetracycline on the microbial assembly and fitness of H. cunea, providing an important reference for a deeper understanding of the complex roles of antibiotics in ecological environments.","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel choice test to detect the influence of fungi on the tunneling behavior of sympatric bark beetles (Coleoptera: Scolytinae). 一种检测真菌对同域树皮甲虫（鞘翅目：鞘翅科）隧道行为影响的新选择试验。

IF 1.8 3区农林科学

Environmental Entomology Pub Date : 2025-06-18 DOI: 10.1093/ee/nvaf055

Andrew P Henning, Richard W Hofstetter

{"title":"A novel choice test to detect the influence of fungi on the tunneling behavior of sympatric bark beetles (Coleoptera: Scolytinae).","authors":"Andrew P Henning, Richard W Hofstetter","doi":"10.1093/ee/nvaf055","DOIUrl":"https://doi.org/10.1093/ee/nvaf055","url":null,"abstract":"Bark beetles are significant forest pests, with primary tree-killing species often relying on obligate mutualistic fungi carried in specialized mycangia. In contrast, secondary bark beetles, which do not typically attack healthy trees, often lack obligate fungal mutualists. However, all bark beetles vector fungi that may provide nutrition to them, improve substrate conditions, or act as antagonists, hindering their success. This study introduces a paired-tube choice test arena to assess bark beetle-fungal interactions using minimal phloem-media. We hypothesized that primary bark beetles with mycangial fungi (eg Dendroctonus frontalis Zimmermann and Dendroctonus barberi Hopkins) avoid phloem infested with nonmycangial fungi such as Ophiostoma minus (Hedgcock) Sydow & P. Sydow, while secondary beetles like Ips pini Say, which do not rely on mycangial fungi, show no preference for fungal-infested or fungus-free phloem. Our findings revealed that D. barberi preferred uninfested phloem, whereas I. pini preferred the O. minus-infested phloem. Interestingly, D. frontalis did not show a preference for either uninfested phloem or O. minus-infested phloem. These results underscore the importance of understanding the effects of fungal symbionts on tunneling behavior, with potential applications in pest management, such as deploying cues from antagonistic fungi as repellents.","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144505156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0