Shahbaz Hussain, Aleena Alam, Misbah Ashraf, Khalid Ali Khan, Hamed A Ghramh, Muneer Abbas, Sohail Abbas, Ri-Zhao Chen
{"title":"Seasonal dynamics and environmental drivers of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in urban Punjab, Pakistan: evidence from larval surveillance.","authors":"Shahbaz Hussain, Aleena Alam, Misbah Ashraf, Khalid Ali Khan, Hamed A Ghramh, Muneer Abbas, Sohail Abbas, Ri-Zhao Chen","doi":"10.1093/ee/nvag010","DOIUrl":"10.1093/ee/nvag010","url":null,"abstract":"<p><p>Aedes aegypti (L.) and Aedes albopictus (Skuse) are the primary vectors of dengue, and understanding their abundance, breeding habitat preferences, and environmental drivers is critical for effective surveillance in endemic regions of Pakistan. A larval surveillance study was conducted across 4 major cities in Punjab, Pakistan (Faisalabad, Lahore, Rawalpindi, and Multan) from 2023 to 2024, focusing on immature stages sampled from various indoor and outdoor containers. Indoor habitats accounted for 53.7% of positive sites, with bath tanks, evaporative coolers, water storage, and discarded tires being the most productive. Outdoor habitats, particularly tube wells and construction sites, accounted for a higher proportion of positive sites in Multan than in the other cities. A. aegypti was the dominant species in all 4 cities; however, the relative contribution of A. albopictus was higher in Rawalpindi and Multan than in Lahore and Faisalabad. The House Index (HI), Container Index, and Breteau Index (BI) exceeded World Health Organization transmission thresholds in Lahore and Faisalabad, with HI and BI being the most significant indicators of infestation, while Rawalpindi and Multan showed lower infestation levels. Seasonal effects were strong, with peak abundance during the monsoon (June to September) and post-monsoon (October to November) periods, coinciding with increased rainfall and humidity. Both species showed significant positive associations with rainfall and relative humidity; however, A. aegypti abundance was more strongly influenced by temperature, whereas A. albopictus was more strongly associated with rainfall and humidity. Community ordination revealed minimal differentiation between cities but significant seasonal shifts in composition. These findings indicate that monsoon-driven climatic conditions are key drivers of Aedes population dynamics in Punjab and highlight the need for intensified, seasonally targeted vector control. Control efforts should prioritize source reduction in highly productive domestic containers and locally important outdoor habitats, particularly before and during the monsoon and extending into the post-monsoon period to reduce dengue transmission risk.</p>","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":"55 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147431662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jhalendra Rijal, Samaneh Sakaki, Sudan Gyawaly, Karla Addesso
{"title":"Optimizing trap design, lure, and color for monitoring Chrysobothris mali (Coleoptera: Buprestidae) in California walnut orchards.","authors":"Jhalendra Rijal, Samaneh Sakaki, Sudan Gyawaly, Karla Addesso","doi":"10.1093/ee/nvag033","DOIUrl":"10.1093/ee/nvag033","url":null,"abstract":"<p><p>Pacific flatheaded borer, Chrysobothris mali (Coleoptera: Buprestidae), is a reemerging pest of walnuts in California. With the expansion of walnut production acres and increased incidences of severe droughts that stress orchards, C. mali has gained economically important pest status in recent years. In this article, we explored different trap designs, colors, and lures for early detection and monitoring of C. mali in commercial walnut orchards in California. In multi-year studies, ground-installed 4-foot-tall purple triangular traps with adhesives on the outer surface were more attractive than the purple panel sticky traps with or without volatile lures. Furthermore, among multiple colors of triangular traps, yellow and red traps captured more C. mali adults compared to other colors, including purple, in replicated trials conducted in 2 walnut orchards. The yellow triangular trap captured adults in these walnut orchards most consistently and earlier in the season. The utility of these traps in monitoring C. mali in walnut orchards and future directions of monitoring research are discussed.</p>","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":"55 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13107124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147697457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Milan Řezáč, Nela Gloríková, Eva Líznarová, Shawn M Wilder, Petr Heneberg
{"title":"The nutritional status of Heliophanus spiders shifts their food preferences for aphid prey over intraguild spider prey.","authors":"Milan Řezáč, Nela Gloríková, Eva Líznarová, Shawn M Wilder, Petr Heneberg","doi":"10.1093/ee/nvaf135","DOIUrl":"10.1093/ee/nvaf135","url":null,"abstract":"<p><p>Spiders are dominant predators of agroecosystems, where they must adapt to regular disturbances. Recent studies show that their abundance in crop fields has declined, even at sites not sprayed with agrochemicals. These declines have been attributed mainly to reduced ecosystem complexity, which decreases herbivore populations and consequently limits the prey available to predators. Moreover, spiders, similar to other arthropods, are sensitive to frequent disturbances, such as plowing and mowing. In addition to these well-established drivers, undernourishment of spiders following repeated anthropogenic disturbance may further contribute to declines. We hypothesized that the food preferences of agrobiont spiders change in response to the previously provided diet. As a model, we used females of Heliophanus cupreus (Walckenaer, 1802) and Heliophanus flavipes (Hahn, 1832) (both Araneae: Salticidae), provided with Drosophila melanogaster Meigen, 1830 of various nutrient compositions, and then provided with a protein-rich intraguild prey represented by Dictyna Sundevaal, 1833 spiders and a lipid-rich agricultural pest prey represented by rose-grain aphids Metopolophium dirhodum (Walker, 1849). The prey preferences of the spiders changed in response to the previously provided diet. The lipid-rich diet increased spider survival and led to a preference for agricultural pest prey. In contrast, spiders on standard or protein-rich diets did not prefer agricultural pest or intraguild prey. Agrobiont spiders are able to adjust the spectrum of captured prey to pests dominating the respective crop type. However, the present findings suggest that the nutritional status of spiders may shape their food preferences, with potential, but untested, implications for predator-prey interactions in agroecosystems.</p>","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13016874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145826984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Roma Durak, Martyna Materowska, Sławomir Bartoszewski, Magdalena Misiorek
{"title":"Temperature-dependent regulation of diapause and hatching success in aphids: implications for climate change.","authors":"Roma Durak, Martyna Materowska, Sławomir Bartoszewski, Magdalena Misiorek","doi":"10.1093/ee/nvag013","DOIUrl":"10.1093/ee/nvag013","url":null,"abstract":"<p><p>Diapause is a crucial adaptive strategy that enables insects, including aphids, to survive unfavorable environmental conditions, particularly during winter. Diapause of aphids takes the form of facultative and embryonic diapause occurring in the eggs. This study investigates the diapause dynamics of aphids, based on Maculolachnus submacula (Walker), at 3 different temperatures to assess the impact of elevated temperatures on embryonic development and hatching success. To determine the diapause strategy and to detect dividing cells, immunostaining was performed. M. submacula exhibited a slow embryonic development strategy during diapause, with mitotic activity and body growth occurring at all tested temperatures. Embryos incubated at higher temperatures achieved greater body length and leg proportions. Higher temperatures accelerated embryonic development, leading to earlier hatching. Hatching success was temperature-dependent, with the highest percentage of hatched individuals at 0 °C and lower success at 10 °C. Premature hatching at higher temperatures resulted in increased rates of developmental abnormalities of the embryo bodies and egg cracking disorder. Survival of nymph rates was low, with mortality reaching 63.5% in natural conditions. These findings highlight the significant influence of temperature on diapause progression, embryonic development, and survival. The conducted studies indicated that an increase in ambient temperature in winter affects aphids with a diapause strategy, characterized by slow development of embryos during winter diapause. Rising global temperatures due to climate change make the understanding of the effects of increasing temperatures on aphid diapause essential for predicting shifts in population dynamics, pest outbreaks, and ecological interactions.</p>","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":"55 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13017630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147389842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bethany Roberton, Cameron Duquette, Jason Harmon, Katherine Kral-O'Brien, Torre Hovick, Dillon Fogarty, Travis Seaborn, Kevin Sedivec
{"title":"Bumble bee communities exhibit delayed recovery following severe drought in rangeland.","authors":"Bethany Roberton, Cameron Duquette, Jason Harmon, Katherine Kral-O'Brien, Torre Hovick, Dillon Fogarty, Travis Seaborn, Kevin Sedivec","doi":"10.1093/ee/nvag028","DOIUrl":"10.1093/ee/nvag028","url":null,"abstract":"<p><p>Rangelands offer resources that support bumble bees (Bombus spp. [Hymenoptera: Apidae]), which are essential pollinators in agricultural and natural ecosystems. However, relatively little is known on how bumble bee communities respond to and recover from drought within rangeland ecosystems. Our study site in North Dakota experienced an extreme drought in 2021, and we investigated the influences of this drought on bumble bee populations over time. Bumble bee abundance was 98% lower during the drought year compared to the pre-drought year. Abundance increased between 2022 and 2024, but all 3 post-drought years were still lower than the pre-drought year. The average temperatures during the current and previous year's growing seasons were most strongly associated with abundance patterns. Community composition differed between the pre- and post-drought periods with 3 species identified as pre-drought indicator species. Although we observed increased floral abundance, normal average temperatures, and non-drought PDSI index values in the first-year post-drought, the bumble bee community experienced a lag effect in abundance and species composition recovery. Our research supports the idea that bumble bee communities can be particularly vulnerable to drought, and their recovery can take multiple years, even when conditions quickly return to normal. We found that species with relatively high pre-drought abundance seemed to recover the quickest following the drought, but more research is needed to understand what makes bumble bee species particularly sensitive or resilient. Information like this can help develop management decisions that protect bumble bee communities as droughts become more frequent and severe in the future.</p>","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":"55 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13043195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147590817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Larissa Pasqualotto, Juarez da Silva Alves, Rubens Antonio Polito, Michele Trombin de Souza, Mireli Trombin de Souza, Vanessa Nogueira Soares, Ana Paula Afonso Schneid da Rosa, Ramiro Ovejero, Renato Jun Horikoshi, Leandro do Prado Ribeiro, Daniel Bernardi
{"title":"Survival and development of Spodoptera frugiperda (Lepidoptera: Noctuidae) strains in different hosts: implication for resistance management.","authors":"Larissa Pasqualotto, Juarez da Silva Alves, Rubens Antonio Polito, Michele Trombin de Souza, Mireli Trombin de Souza, Vanessa Nogueira Soares, Ana Paula Afonso Schneid da Rosa, Ramiro Ovejero, Renato Jun Horikoshi, Leandro do Prado Ribeiro, Daniel Bernardi","doi":"10.1093/ee/nvag002","DOIUrl":"10.1093/ee/nvag002","url":null,"abstract":"<p><p>The fall armyworm Spodoptera frugiperda (J. E. Smith, 1797) has evolved resistance to different Bt maize technologies carrying Bacillus thuringiensis genes. One hypothesis for the rapid evolution of resistance may be associated with the species' ability to multiply and survive on alternative host plants, especially during the off-season of the main crops. The aim of this study was to evaluate the biological parameters and fertility life table of susceptible (SS), heterozygous (RS) and resistant (RR) strains of S. frugiperda to Bt YieldGard VT PRO maize when kept in summer-winter cover crops. Based on the results, S. frugiperda did not survive on weeds Barnyardgrass (Echinochloa crus-galli). SS, RR, and RS larvae fed on white oats (Avena sativa), ryegrass (Lolium multiflorum), maize (non-Bt isoline) showed an egg to adult generation of approximately 30 d. The RS strain performed better than both the SS and RR strains, this is consistent with the individuals' high heterosis index. In addition, the insects reared on these hosts showed the highest reproductive rates (Ro = 496.2 to 578.1) and growth rates (rm = 0.143 to 0.185). In contrast, sorghum (Sorghum bicolor) or Persian clover (Trifolium resupinatum) prolonged the cycle by 4 d and reduced egg-to-adult viability parameters by 13%, larval weight (0.057 to 0.74 mg), pupal weight (0.36 to 0.86 mg), Ro (196.4 to 324.0), and rm (0.104 to 0.122). However, it is worth noting that all the host plants evaluated, except for Barnyardgrass, can serve to multiply S. frugiperda in the field and favor the reestablishment of the insect during the corn off-season and, consequently, accelerate the evolution of the pest's resistance.</p>","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146118238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nicole I Bailey, Nathan V Comai, Nancy A Bartholomew, John M Mola
{"title":"Local-scale factors predict wild bee richness, whereas landscape-scale factors predict butterfly richness in city parks.","authors":"Nicole I Bailey, Nathan V Comai, Nancy A Bartholomew, John M Mola","doi":"10.1093/ee/nvag003","DOIUrl":"10.1093/ee/nvag003","url":null,"abstract":"<p><p>Urbanization has a growing influence on pollinator species worldwide, benefiting some species while threatening others. Urban areas have high cover of impervious surfaces, altered floral communities, and competition from non-native species. Additionally, the existing biodiversity in urban areas may not be equitably distributed, instead reflecting patterns of wealth (ie luxury effects). Despite these challenges, city parks can serve as refuges for pollinator biodiversity. We investigated how wild bee and butterfly species richness and community composition differed across city parks depending on landscape-scale (neighborhood income and land cover surrounding a park) and local-scale (floral traits and honey bee abundance) factors in Denver, Colorado, United States. We found increased wild bee richness was primarily related to local-scale factors like increases in floral species richness and non-weedy plant cover. Butterfly richness was instead primarily negatively associated with landscape-scale factors like increased cover of impervious surfaces and income. Although we did not observe broad-scale luxury effects, we found that lower-income parks had a higher proportion of non-native weedy plants and lower wild bee species richness than higher-income parks, which had primarily native plantings and higher wild bee richness. Wild bee communities differed between parks with high and low abundances of honey bees (Apis mellifera), with Bombus huntii most associated with abundant honey bee parks, and 7 solitary bees associated with lower honey bee parks. These findings suggest managers can implement species-rich native plantings in parks to support wild bee diversity regardless of landscape context. However, interventions to support butterflies may rely more on landscape-level planning.</p>","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146178503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abeer Albalawneh, Sami Faisal Alarsan, Heba Hasan, Mai Diab, Saja Abu Znaimah, Yazan AlBalawnah, Ehab Alnaimat
{"title":"Bioconversion potential of tomato, corn, and date palm wastes by black soldier fly larvae: a comparative evaluation.","authors":"Abeer Albalawneh, Sami Faisal Alarsan, Heba Hasan, Mai Diab, Saja Abu Znaimah, Yazan AlBalawnah, Ehab Alnaimat","doi":"10.1093/ee/nvag008","DOIUrl":"10.1093/ee/nvag008","url":null,"abstract":"<p><p>Black soldier fly larvae (Hermetia illucens L.; BSFL) are increasingly used for organic waste valorization; however, the suitability of 100% non-food agricultural residues common in arid and semi-arid systems remains insufficiently tested without supplementation. This study compared growth performance, bioconversion efficiency, and nutrient outputs of BSFL reared for 14 d on chicken feed (control) versus 100% corn, tomato, and date palm wastes. Larvae were reared under controlled conditions in a completely randomized design (n = 3 per treatment). Growth, substrate reduction, bioconversion efficiency, larval composition, and frass properties were measured. Data were analyzed using 1-way analysis of variance with Tukey's honest significant difference (HSD) (α = 0.05). Chicken feed produced the highest final larval weight (0.166 ± 0.010 g), while corn waste supported intermediate growth (0.070 ± 0.004 g). Tomato and date palm wastes resulted in minimal final weights (0.011 ± 0.001 g and 0.014 ± 0.002 g), indicating failure to support development when used alone. Substrate reduction and bioconversion efficiency were highest in the control, intermediate in corn waste, and lowest in date palm waste. Larval protein content was highest in chicken feed and tomato waste, intermediate in corn waste, and lowest in date palm waste. Tomato-derived frass showed elevated electrical conductivity and chloride, suggesting salinity risk limiting direct soil application without dilution. Corn waste showed the greatest potential for BSFL bioconversion without supplementation. Tomato and date palm wastes in pure form were unsuitable and likely require pretreatment and/or co-substrates to enable larval growth and safer frass use.</p>","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147321569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
John W Mahas, Alan E Wilson, Todd D Steury, Alana L Jacobson
{"title":"Association of plant pathogen vectors and vector-borne plant pathogens with landscape composition: a meta-analysis.","authors":"John W Mahas, Alan E Wilson, Todd D Steury, Alana L Jacobson","doi":"10.1093/ee/nvaf120","DOIUrl":"10.1093/ee/nvaf120","url":null,"abstract":"<p><p>Previous studies investigating the relationship between landscape composition surrounding a crop, and either plant pathogen vectors or vector-borne plant pathogens in the crop, have not observed consistent relationships, and no quantitative synthesis of the current literature has been conducted. We conducted a meta-analysis of 289 published effect sizes from 28 studies to better understand the relationships between landscape composition and plant pathogen vectors or vector-borne pathogens, while considering a series of biological, ecological, and environmental factors that may contribute to variation in the relationships. We found that vector and vector-borne pathogens were positively associated with landscape composition metrics, including the percentages of the landscape surrounding the study areas that were crop, non-crop, and natural habitat but not with habitat diversity. Vector host plant specialists exhibited a significant positive association with various landscape composition metrics, while host plant generalists did not, supporting the idea that specialists are more dependent upon specific host landscape features, whereas generalists have hosts that are generally more abundant across various landscapes. Measurements of both abundance and incidence of pests exhibited significant positive associations with landscape composition metrics. For both persistent and non-persistent pathogens, the positive association between pathogens and landscape composition became stronger at larger spatial scales (500 to 10,000 m). This meta-analysis demonstrated that increasing the percentage of crop, non-crop, or natural habitat within a landscape can increase vectors or vector-borne pathogens in the crop. However, the specific landscape feature that contributes to increased pest abundance or incidence will depend on the crop-pest system.</p>","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12818966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145530598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Artificial adult diet for Chrysopa oculata (Neuroptera: Chrysopidae) to overcome the barriers to rearing predatory adult green lacewings.","authors":"Binita Sigdel, Kaushalya G Amarasekare","doi":"10.1093/ee/nvag012","DOIUrl":"10.1093/ee/nvag012","url":null,"abstract":"<p><p>The green lacewing Chrysopa oculata Say is a crucial natural enemy contributing to arthropod pest management in crop production. Chrysopa spp. are unique because both larvae and adults are predatory. In contrast, only the larvae of Chrysoperla spp. are predatory, and their adults consume nectar and pollen. Although an established artificial diet exists for Chrysoperla adults, limited information on suitable artificial diets for Chrysopa adults limits their broader use in research and commercial biological control programs. Thus, the objective of this study was to identify a suitable diet for Chrysopa oculata adults. Five protein supplements: cricket powder, fly fish food, goldfish food, aphids, and Ephestia kuehniella Zeller eggs added separately to the Chrysoperla carnea (Stephens) adult diet were evaluated as C. oculata adult diet treatments. We provided the treatments to paired C. oculata adults for 6 consecutive generations and evaluated egg hatch, larval survival, developmental time, adult longevity, and sex ratio. Chrysopa oculata in the goldfish and fly fish food treatments had higher egg-to-adult survival compared to those in the E. kuehniella treatment in the second generation. However, the later generations showed significant effects of the treatments, with no adults eclosed from the fly fish food treatment, and significantly fewer adults eclosed under the goldfish food treatment in the fifth generation. Additionally, none of the eggs hatched in the cricket powder treatment in the seventh generation. The E. kuehniella treatment supported a higher number of generations, indicating E. kuehniella as the most suitable protein supplement for C. oculata adults.</p>","PeriodicalId":11751,"journal":{"name":"Environmental Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}