Entomologia Experimentalis et Applicata最新文献_第4页

Regulation of trehalose metabolism mediated by validamycin on chitin synthesis in Spodoptera frugiperda 有效性霉素介导海藻糖代谢对褐夜蛾几丁质合成的调控

IF 1.4 3区农林科学

Entomologia Experimentalis et Applicata Pub Date : 2024-08-28 DOI: 10.1111/eea.13510

Yan-qi Yang, Yu-jian Liang, Jun-rui Zhi, Ding-yin Li, Cao Li

{"title":"Regulation of trehalose metabolism mediated by validamycin on chitin synthesis in Spodoptera frugiperda","authors":"Yan-qi Yang, Yu-jian Liang, Jun-rui Zhi, Ding-yin Li, Cao Li","doi":"10.1111/eea.13510","DOIUrl":"https://doi.org/10.1111/eea.13510","url":null,"abstract":"Trehalase (TRE) is a key enzyme for degrading trehalose, which plays a vital role in the growth and development of insects. Although validamycin, a compound belonging to a class of efficient antibiotics and fungicides, can control pests by suppressing TRE activities, it remains unknown whether it acts on both trehalose and chitin metabolism in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), a major pest of maize (Zea mays L., Poaceae). This study investigated the changes in trehalose metabolism after validamycin treatment in S. frugiperda and its effects on the downstream chitin synthesis pathway. Compared with the control, S. frugiperda exhibited varying degrees of mortality after treatment with four concentrations of validamycin, showing a dose-dependent increase in mortality rate. The mortality rates 24 and 48 h after treatment with 0.07 mg μL−1 validamycin were 35.6% and 42.2%, respectively, indicating the effective lethal concentration. Treatment with 0.07 mg μL−1 validamycin led to developmental delay, abnormal molting, and death in S. frugiperda, but it exerted no lasting effects on the survival rate, pupal weight, and phenotype during its subsequent developmental stages. At 24 h after validamycin treatment, TRE1 and TRE2 activities and glucose content decreased significantly, whereas the trehalose content increased significantly. Treatment with validamycin significantly upregulated TRE1 and TRE2 expression after 24 and 48 h and downregulated the mRNA expression of chitin synthase A and B genes. However, after 72 h, chitin content was not significantly affected. Hence, validamycin can destroy the dynamic transformation balance of trehalose and glucose by inhibiting the activities of the two TREs, and further affect the expression of downstream chitin synthase genes. These findings provide a theoretical basis for using TRE inhibitors to control S. frugiperda.","PeriodicalId":11741,"journal":{"name":"Entomologia Experimentalis et Applicata","volume":"173 1","pages":"2-11"},"PeriodicalIF":1.4,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arthropod activity density and predation are supported by mixed cropping of maize with common sainfoin (Onobrychis viciifolia) and reduced tillage 玉米与普通莎草（Onobrychis viciifolia）混作并减少耕作，有利于提高节肢动物的活动密度和捕食率

IF 1.4 3区农林科学

Entomologia Experimentalis et Applicata Pub Date : 2024-08-19 DOI: 10.1111/eea.13508

Bastian Häfner, Gunda Schulte auf’m Erley, Doreen Gabriel, Andra Herden, Frank Höppner, Jens Dauber, Fabian Nürnberger

{"title":"Arthropod activity density and predation are supported by mixed cropping of maize with common sainfoin (Onobrychis viciifolia) and reduced tillage","authors":"Bastian Häfner, Gunda Schulte auf’m Erley, Doreen Gabriel, Andra Herden, Frank Höppner, Jens Dauber, Fabian Nürnberger","doi":"10.1111/eea.13508","DOIUrl":"https://doi.org/10.1111/eea.13508","url":null,"abstract":"Mixed cropping with legumes may mitigate negative impacts of maize, Zea mays L. (Poaceae), production on arthropods by providing additional habitat structures and food resources. Still, knowledge about effects of less common legume partner crops for mixed cropping of maize is scarce. Activity density of epigeic predatory arthropods and proxies for ecosystem functions related to biological pest control were assessed on an experimental field. Plots of mixed cropping of maize and sainfoin, Onobrychis viciifolia Scop. (Fabaceae), and respective single plant species (plot size: 6 × 5 m; n = 48) differed in combination of the seed rate of each partner crop and tillage treatment. Using the “rapid ecosystem function assessment” approach in each plot on three occasions, we tested whether the activity density of generalist arthropod predators and their associated ecosystem functions can be promoted by mixed cropping of maize with sainfoin. Our data show that, compared with tilled maize as a pure culture, mixed cropping can increase activity density of generalist arthropod predators and insect predation potential but effects were limited to strip-tilled cropping systems and partly depended on sampling date. Thus, from an ecological viewpoint, mixed cropping of sainfoin and maize in strip-till systems offers the possibility to promote predatory insects and their potential for pest regulation. However, this system is inherently more prone to weed occurrence. Competition effects between cropping partners and weeds may reduce yields and hence lead to trade-offs between enhancement of arthropods and economic viability.","PeriodicalId":11741,"journal":{"name":"Entomologia Experimentalis et Applicata","volume":"172 11","pages":"1098-1111"},"PeriodicalIF":1.4,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eea.13508","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of assisted migration: An introduced herbivore has short-term and long-term effects on its native host plant population 辅助迁移的影响：引入的食草动物会对其本地寄主植物种群产生短期和长期影响

IF 1.4 3区农林科学

Entomologia Experimentalis et Applicata Pub Date : 2024-08-19 DOI: 10.1111/eea.13507

Nitin Ravikanthachari, Libby L. Burch, Rachel E. Powell, Danielle M. Scott, Charlotte R. Wayne, Kristjan Niitepõld, Risa H. Rosenberg, Carol L. Boggs

{"title":"Impacts of assisted migration: An introduced herbivore has short-term and long-term effects on its native host plant population","authors":"Nitin Ravikanthachari, Libby L. Burch, Rachel E. Powell, Danielle M. Scott, Charlotte R. Wayne, Kristjan Niitepõld, Risa H. Rosenberg, Carol L. Boggs","doi":"10.1111/eea.13507","DOIUrl":"https://doi.org/10.1111/eea.13507","url":null,"abstract":"Assisted migration consists of the introduction of a species to previously inhabited areas or to new suitable regions. Such introductions have been touted as a viable tool for conserving the earth's biodiversity. However, both the likely success of assisted migrations and the impacts on local communities are hotly debated. Empirical data on the local impacts of assisted migration are particularly lacking. We examined the short and long time-scale effects of herbivory on Lonicera involucrata (Richards) Banks ex. Spreng (Caprifoliaceae) after an introduction of Euphydryas gillettii Barnes (Lepidoptera: Nymphalidae, Melitaeini) to Gunnison County, Colorado, USA, via an assisted migration in 1977. The plant is the primary larval host plant for the butterfly. We quantified plant seed production, plant survival, and population stage structure in two sets of observational experiments. We found that herbivory by E. gillettii increased L. involucrata reproduction on an annual time scale, independent of plant size and local microhabitat characteristics. Over the time since the butterfly's introduction, herbivory by E. gillettii resulted in a plant population structure biased toward smaller plants in the butterfly introduction and satellite sites compared with sites without the butterfly. Our results highlight the importance of studying the effects of assisted migrations on native populations at different temporal scales. As assisted migration becomes an indispensable tool for species conservation, our work adds to the understanding of the multi-trophic impacts of assisted introductions on local populations and communities.","PeriodicalId":11741,"journal":{"name":"Entomologia Experimentalis et Applicata","volume":"172 11","pages":"982-991"},"PeriodicalIF":1.4,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eea.13507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ontogenetic transfer of microplastics in natural populations of malaria mosquitoes in Western Siberia 微塑料在西西伯利亚疟疾蚊子自然种群中的遗传转移

IF 1.4 3区农林科学

Entomologia Experimentalis et Applicata Pub Date : 2024-08-16 DOI: 10.1111/eea.13509

Anastasia V. Simakova, Anna A. Varenitsina, Irina B. Babkina, Yulia V. Andreeva, Yulia A. Frank

{"title":"Ontogenetic transfer of microplastics in natural populations of malaria mosquitoes in Western Siberia","authors":"Anastasia V. Simakova, Anna A. Varenitsina, Irina B. Babkina, Yulia V. Andreeva, Yulia A. Frank","doi":"10.1111/eea.13509","DOIUrl":"https://doi.org/10.1111/eea.13509","url":null,"abstract":"The uptake, accumulation, and ontogenetic transfer of microplastics (MPs) in bloodsucking mosquitoes of the genus Anopheles, vectors of helminth and protozoa parasites in humans and animals, were studied under laboratory conditions. In the experiment, 2-μm-diameter fluorescent polystyrene spheres were counted in mosquitoes of all stages, from larvae to adults. Larvae from a natural population readily ingested MPs with food, accumulating on average 3.9 × 106 particles per larva within 3 days. The concentration of MPs decreased significantly in mosquitoes during their transition from stage to stage. The average number of detected MPs attained 110 particles per pupa and 3.0 particles per adult. MP uptake by mosquitoes did not affect their survival, whereas the rate of metamorphosis in MP-consuming mosquitoes was slightly higher. Our data confirmed that MPs can be transferred from feeding larvae to non-feeding pupae and adult flying Anopheles mosquitoes from natural populations in aquatic ecosystems.","PeriodicalId":11741,"journal":{"name":"Entomologia Experimentalis et Applicata","volume":"172 11","pages":"1046-1053"},"PeriodicalIF":1.4,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prohydrojasmon treatment of Brassica juncea alters the performance and behavioural responses of the cabbage aphid, Brevicoryne brassicae 原氢茉莉酮处理芥蓝可改变菜蚜 Brevicoryne brassicae 的表现和行为反应

IF 1.4 3区农林科学

Entomologia Experimentalis et Applicata Pub Date : 2024-08-16 DOI: 10.1111/eea.13506

Jamin Ali, Feng Xiao, Aleena Alam, Liu Jia Li, YunLiang Ji, Wu Hai Chao, Qin Weibo, Aocong Xie, Bi Zengyi, Mogeda M. Abdel Hafez, Hamed A. Ghramh, Khalid Ali Khan, Adil Tonğa, Rizhao Chen

{"title":"Prohydrojasmon treatment of Brassica juncea alters the performance and behavioural responses of the cabbage aphid, Brevicoryne brassicae","authors":"Jamin Ali, Feng Xiao, Aleena Alam, Liu Jia Li, YunLiang Ji, Wu Hai Chao, Qin Weibo, Aocong Xie, Bi Zengyi, Mogeda M. Abdel Hafez, Hamed A. Ghramh, Khalid Ali Khan, Adil Tonğa, Rizhao Chen","doi":"10.1111/eea.13506","DOIUrl":"https://doi.org/10.1111/eea.13506","url":null,"abstract":"Plants encounter a myriad of biotic and abiotic stresses throughout their life cycle, among which aphids pose a significant challenge as herbivorous insect pests. Aphids cause both direct damage through feeding and indirect damage by transmitting viruses. Given the growing concerns about insecticide resistance and the non-target effects of synthetic insecticides, there is an urgent need to develop novel pest control strategies that are dependent on plant defence strategies. In this study, we assessed the effects of exogenous prohydrojasmon (PDJ), a derivative of jasmonic acid, at 24 h (PDJ24) and 48 h (PDJ48) post-treatment on the cabbage aphid, Brevicoryne brassicae L. (Hemiptera: Aphididae), feeding on Brassica juncea L. (Brassicaceae). The impact of PDJ treatments was assessed by conducting performance bioassays (survival and fecundity) and behavioural bioassays (settlement and attraction to volatiles in an olfactometer). Our findings reveal that the exogenous application of PDJ did not significantly affect on the performance and behaviour of B. brassicae at 24-h post-treatment. However, PDJ treatment significantly reduced the performance of B. brassicae at 48-h post-treatment, and affected the behaviour in terms of reduced settlement on and attraction to volatiles of PDJ-treated plants. These results suggest that PDJ has the potential to effectively induce defences in Brassica against sucking pests. This study provides valuable insights into possibility of employing PDJ as an alternative approach for sustainable pest management in agriculture.","PeriodicalId":11741,"journal":{"name":"Entomologia Experimentalis et Applicata","volume":"172 11","pages":"1014-1023"},"PeriodicalIF":1.4,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bait type affects the diversity assessment of cetoniid beetles in the Brazilian Cerrado 饵料类型对巴西塞拉多地区甲壳虫多样性评估的影响

IF 1.4 3区农林科学

Entomologia Experimentalis et Applicata Pub Date : 2024-08-08 DOI: 10.1111/eea.13505

Juliane Evangelista, Maria Carolina Blassioli-Moraes, Raúl Alberto Laumann, Miguel Borges, Charles Martins de Oliveira, Marina Regina Frizzas

{"title":"Bait type affects the diversity assessment of cetoniid beetles in the Brazilian Cerrado","authors":"Juliane Evangelista, Maria Carolina Blassioli-Moraes, Raúl Alberto Laumann, Miguel Borges, Charles Martins de Oliveira, Marina Regina Frizzas","doi":"10.1111/eea.13505","DOIUrl":"10.1111/eea.13505","url":null,"abstract":"Cetoniidae (Coleoptera), known as flower and fruit beetles, form a diverse group, feed on nectar, pollen, plant exudates, and are highly attracted to fermented fruits. To evaluate their diversity in natural and agricultural areas, traps baited with fermented fruits are used. The objective of this study was to compare the effectiveness of various types of fruit used as bait, with or without the addition of sugarcane juice to accelerate fruit fermentation, on the assessment of Cetoniidae diversity in the Brazilian Cerrado. The study was conducted in two conservation units in Brasília (DF, Brazil). Two experiments were conducted, the first aiming to select the most attractive fruits (banana, pineapple, mango, grape, or cagaita), and the second to verify whether there is interference of sugarcane juice in the fruit fermentation process and, consequently, in the attractiveness to Cetoniidae. The chemical profiles of the types of bait were evaluated by identifying and quantifying the main volatile organic compounds released by the fruit traps. Insect collections were conducted weekly using fruit-baited traps for 1 month, without interruption. We found that the diversity estimate of Cetoniidae was higher for banana-based baits relative to grape and cagaita fruit, but not relative to pineapple, mango, and sugarcane juice. Additionally, no increase in attractiveness was observed with the addition of sugarcane juice to banana, pineapple, and mango bait. Most compounds in the fruit extracts belong to alcohol, ester, and terpene functional groups. Our results suggest that banana, pineapple, mango, and sugarcane juice, used alone, may be used for Cetoniidae diversity studies, and that banana bait seems to be the most suitable for Cetoniidae collection in the Cerrado, because it enables the collection of a high diversity of species and also of species considered rare.","PeriodicalId":11741,"journal":{"name":"Entomologia Experimentalis et Applicata","volume":"172 11","pages":"1082-1097"},"PeriodicalIF":1.4,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141929689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VibePy: An open-source tool for conducting high-fidelity vibrational playback experiments VibePy：用于进行高保真振动回放实验的开源工具

IF 1.4 3区农林科学

Entomologia Experimentalis et Applicata Pub Date : 2024-08-08 DOI: 10.1111/eea.13500

Alana J. Evora, Reginald B. Cocroft, Shyam Madhusudhana, Jennifer A. Hamel

{"title":"VibePy: An open-source tool for conducting high-fidelity vibrational playback experiments","authors":"Alana J. Evora, Reginald B. Cocroft, Shyam Madhusudhana, Jennifer A. Hamel","doi":"10.1111/eea.13500","DOIUrl":"10.1111/eea.13500","url":null,"abstract":"Many insect species communicate about activities central to their survival and reproduction via vibrational signals (i.e., waves that travel through solid substrates). One widely used and effective approach for testing hypotheses about communication is the playback experiment, in which stimuli are played to focal organisms and their responses are documented. Recent technological advances and efforts by vibrational communication researchers have diversified the hardware options available for use in such experiments, but proprietary software is still needed for high-fidelity playbacks. Here, we present an open-source software tool, VibePy, that (1) measures and compensates for undesired filtering and (2) calibrates playback amplitude. Because proprietary software licenses impose economic barriers that can limit access to research, we have developed VibePy in the open-source language Python. The functions provided by VibePy are a stepping stone toward increasing access to vibration research. Because the tool is open-source, we hope that the software will be expanded upon by others in the community of researchers studying vibrational communication and insect behavior.","PeriodicalId":11741,"journal":{"name":"Entomologia Experimentalis et Applicata","volume":"172 12","pages":"1176-1183"},"PeriodicalIF":1.4,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eea.13500","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141926073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variation in immune response in the generalist herbivore fall webworm across four common host plants 食草动物秋网虫对四种常见寄主植物的免疫反应差异

IF 1.4 3区农林科学

Entomologia Experimentalis et Applicata Pub Date : 2024-08-02 DOI: 10.1111/eea.13502

Audrey M. Mitchell, Dhaval K. Vyas, Shannon M. Murphy

{"title":"Variation in immune response in the generalist herbivore fall webworm across four common host plants","authors":"Audrey M. Mitchell, Dhaval K. Vyas, Shannon M. Murphy","doi":"10.1111/eea.13502","DOIUrl":"https://doi.org/10.1111/eea.13502","url":null,"abstract":"Dietary generalist herbivorous insects are widespread and often occur in a variety of environments. Across their geographic range, herbivorous insects may encounter variable plant traits as they feed on high-quality or low-quality plants. Herbivorous insect larvae experience both bottom-up (host plant) and top-down (parasitoid) factors that affect survival. Host plant quality may affect larval growth and survival in that larvae feeding on low-quality plants often suffer reduced fitness. However, herbivores on different host plants are also subject to different levels of parasitism. High-quality plants confer stronger larval performance (higher survival, more offspring), but larvae may also face higher parasitism. In some herbivore species, diet mediates larval immune response. The generalist insect herbivore fall webworm (FW), Hyphantria cunea Drury (Lepidoptera: Erebidae), is a moth native to North America, and its larvae have considerable variance in their performance when reared on different host plants. We investigated whether diet affects the immune response in FW larvae when they are reared on different host plant species known to vary in food quality. We measured immune response by melanization of a nylon filament. We found significant differences in immune response across host plants, indicating that diet mediates immune response in FW larvae. Our study helps elucidate the factors that cause variation in immune response in a generalist herbivore.","PeriodicalId":11741,"journal":{"name":"Entomologia Experimentalis et Applicata","volume":"172 11","pages":"1008-1013"},"PeriodicalIF":1.4,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying the complex transmission of substrate-borne vibrations with scanning laser vibrometry 利用扫描激光测振仪量化基底振动的复杂传递过程

IF 1.4 3区农林科学

Entomologia Experimentalis et Applicata Pub Date : 2024-08-02 DOI: 10.1111/eea.13501

Rowan H. McGinley, Reginald B. Cocroft, Damian O. Elias, Elizabeth Redle, Jenna Gorlewicz, Kasey D. Fowler-Finn

{"title":"Quantifying the complex transmission of substrate-borne vibrations with scanning laser vibrometry","authors":"Rowan H. McGinley, Reginald B. Cocroft, Damian O. Elias, Elizabeth Redle, Jenna Gorlewicz, Kasey D. Fowler-Finn","doi":"10.1111/eea.13501","DOIUrl":"https://doi.org/10.1111/eea.13501","url":null,"abstract":"Substrate-borne vibrations are ubiquitous in nature and are used by diverse taxa to communicate and to obtain information about their environments. However, substrate-borne vibrations remain understudied compared with other sensory and signaling modalities, in part due to human sensory biases. In addition, understanding and quantifying the transmission of vibrations remains a challenging task due to it being dependent on both signal properties and properties of the substrates that the signals transmit through. Here, we provide methods for playing back and measuring the transmission of vibrations throughout a substrate. Using linear resonant actuators, we conducted playbacks of pure tones and frequency sweeps on wooden dowels and on the stems of potted Ptelea trifoliata L. (Rutaceae) plants. We used scanning laser Doppler vibrometry to measure the signals at multiple locations along the length of the dowels and plant stems. We demonstrate that playback of a frequency sweep yields more data in a shorter amount of time than multiple playbacks and measurements of pure tone signals. Our results are also consistent with previous findings showing that signals produce frequency and location specific minima and maxima (nodes and antinodes) throughout the substrates, rather than simply attenuating with distance. This results in filtering of signals, such that their spectra are unique at any given measurement location—illustrating the importance of measuring vibrations at multiple locations. We discuss the implications of such filtering phenomena for vibrationally signaling animals and the biotremologists that study them.","PeriodicalId":11741,"journal":{"name":"Entomologia Experimentalis et Applicata","volume":"172 12","pages":"1184-1195"},"PeriodicalIF":1.4,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eea.13501","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection of sheep skin bacteria to reduce blood feeding by biting midges under laboratory conditions 在实验室条件下选择羊皮细菌以减少咬蠓的吸血量

IF 1.4 3区农林科学

Entomologia Experimentalis et Applicata Pub Date : 2024-08-01 DOI: 10.1111/eea.13503

Paula S. Brok, Stéphanie M. Jost, Niels O. Verhulst

{"title":"Selection of sheep skin bacteria to reduce blood feeding by biting midges under laboratory conditions","authors":"Paula S. Brok, Stéphanie M. Jost, Niels O. Verhulst","doi":"10.1111/eea.13503","DOIUrl":"https://doi.org/10.1111/eea.13503","url":null,"abstract":"Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) are of huge veterinary importance, mainly as vectors of pathogens, such as Bluetongue virus. Currently, there are no effective methods to protect animals against biting midges, as insecticides have limited or short-lived efficacy. Biting midges are attracted to hosts by carbon dioxide and by their body odours, which are mainly produced by skin bacteria. In humans, it has been shown that differences between individuals in attractiveness to mosquitoes is mediated by these skin bacterial volatiles. This opens the possibility to protect individuals from biting insects by supplementing their skin microbiome with probiotics. In this study, we investigated this approach by culturing sheep skin bacteria on various media and assessing their effects against field-caught Culicoides (overwhelmingly Obsoletus group species) as well as laboratory-reared Culicoides nubeculosus (Meigen). Aerococcus urinaeequi, Bacillus safensis, Bacillus subtilis, Jeotgalicoccus psychrophilus, Micrococcus sp. and Staphylococcus equorum were selected to be tested in a dual-choice Y-tube olfactometer, assessing their behavioural effects on biting midges. We revealed an avoidance effect on laboratory-reared C. nubeculosus when testing B. safensis and B. subtilis. Bacillus safensis and Micrococcus sp. yielded significant repellent potential towards field-caught Culicoides. These two candidates were subsequently tested in a membrane blood-feeding assay. When the bacterial species B. safensis was applied to the membrane, a feeding reduction of 83% was observed with field-caught Culicoides.","PeriodicalId":11741,"journal":{"name":"Entomologia Experimentalis et Applicata","volume":"172 11","pages":"1062-1071"},"PeriodicalIF":1.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/eea.13503","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142435082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0