Entomologia Generalis最新文献_第5页

Host plants expressing pxy-miR-34-5p demonstrate high efficacy against multiple lepidopterous pests 表达 pxy-miR-34-5p 的寄主植物对多种鳞翅目害虫具有很高的防治效果

IF 6.9 1区农林科学

Entomologia Generalis Pub Date : 2024-07-04 DOI: 10.1127/entomologia/2024/2416

Linhong Li, Tianxin Guo, Bin Zhu, Pei Liang

{"title":"Host plants expressing pxy-miR-34-5p demonstrate high efficacy against multiple lepidopterous pests","authors":"Linhong Li, Tianxin Guo, Bin Zhu, Pei Liang","doi":"10.1127/entomologia/2024/2416","DOIUrl":"https://doi.org/10.1127/entomologia/2024/2416","url":null,"abstract":"Lepidopteran pests are destructive pests and have evolved significant resistance to many insecticides. Alternative green control strategies are urgently needed. Our previous research had revealed that overexpression or knockdown of a Plutella xylostella microRNA, pxy-miR-34-5p, could lead to high mortality and severe developmental defects in multiple lepidopteran pests, thus has great potential in lepidopteran pests control. Here, pxy-miR-34-5p was successfully expressed in cruciferous plants, Arabidopsis thaliana and Brassica campestris. Feeding on leaves of transgenic miR-34-5p rape significantly reduced the expression of ecdysone receptor gene PxEcR of P. xylostella by 56.8–70.2%. Bioassays results demonstrated that compared to that of the larvae fed on wild type Arabidopsis, the accumulated mortalities of P. xylostella fed on transgenic miR-34-5p Arabidopsis were significantly increased by 34.9%–52.0%. After feeding on transgenic miR-34-5p rape leaves for four to six days, the highest accumulated corrected mortalities of P. xylostella, Spodoptera frugiperda and Spodoptera exigua larvae reached 100.0%, 94.1%, and 100.0%, respectively. These results indicated that insect microRNAs can be used to control multiple target pests by constructing transgenic crops, which lays an important foundation for the further development of miRNA-based green pest control strategies.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141567278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flavonoids mediate systemic defenses induced by root feeding in cotton 类黄酮介导棉花根食诱导的系统防御能力

IF 6.9 1区农林科学

Entomologia Generalis Pub Date : 2024-07-04 DOI: 10.1127/entomologia/2024/2482

Qian Zhang, Qiongqiong Wang, Kris A. G. Wyckhuys, Shuangxia Jin, Yanhui Lu

{"title":"Flavonoids mediate systemic defenses induced by root feeding in cotton","authors":"Qian Zhang, Qiongqiong Wang, Kris A. G. Wyckhuys, Shuangxia Jin, Yanhui Lu","doi":"10.1127/entomologia/2024/2482","DOIUrl":"https://doi.org/10.1127/entomologia/2024/2482","url":null,"abstract":"Plant-herbivore interactions dominate food web links in terrestrial settings, thereby shaping ecosystem structure and functioning. Within a given plant, both primary and secondary metabolites mediate the interplay between above- and below-ground herbivores. Here, we investigate how root feeding by larvae of the turnip moth Agrotis segetum (Lepidoptera: Noctuidae) affects development and feeding behavior of an above-ground sap-feeder i.e., the large cotton aphid Acyrthosiphon gossypii (Hemiptera: Aphididae) on cotton. We further draw upon transcriptomics, metabolomics and in-vitro bioassays to elucidate how plant metabolites mediate these interactions. Root feeding reduces A. gossypii adult survival and fecundity by 35.1%. Aphids on A. segetum-infested cotton spend 7% longer probing cells than on un-infested plants. Root and stem herbivory by A. segetum larvae alters gene transcripts and metabolites in leaves, inducing biosynthesis of three flavonoids i.e., dihydromyricetin, phloridzin and dihydroquercetin. In-vitro bioassays show that the latter two compounds markedly decrease A. gossypii adult survival and fecundity. Below-ground herbivory thus elicits biosynthesis and systemic translocation of phloridzin and dihydroquercetin, with antagonistic effects on above-ground sap-feeders. Our study sheds light on the underlying mechanisms of herbivore-triggered plant defenses in cotton and reveals the interplay between herbivore guilds across ecological realms. We discuss the implications of these findings for pest management.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141567389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pruning can recover the health of wild apple forests attacked by the wood borer Agrilus mali in central Eurasia 修剪可以恢复欧亚大陆中部受木蠹蛾 Agrilus mali 侵害的野生苹果林的健康

IF 6.9 1区农林科学

Entomologia Generalis Pub Date : 2024-07-04 DOI: 10.1127/entomologia/2024/2297

Ping Zhang, Yifan Li, Ting Wang, Xin Zhang, Yanlong Zhang, Hao Xu, Roman Jashenko, Zhaoke Dong, Myron P. Zalucki, Zhaozhi Lu

{"title":"Pruning can recover the health of wild apple forests attacked by the wood borer Agrilus mali in central Eurasia","authors":"Ping Zhang, Yifan Li, Ting Wang, Xin Zhang, Yanlong Zhang, Hao Xu, Roman Jashenko, Zhaoke Dong, Myron P. Zalucki, Zhaozhi Lu","doi":"10.1127/entomologia/2024/2297","DOIUrl":"https://doi.org/10.1127/entomologia/2024/2297","url":null,"abstract":"The invasive apple wood borer Agrilus mali Matsumura (Coleoptera: Buprestidae) threatens the viability of wild apple forests in Xinjiang, China, imperiling the preservation of apple germplasm resources in Central Eurasia. Pruning infested branches can reduce the likelihood of outbreaks of various pests and associated damage, but its efficacy and feasibility for managing A. mali has not been well-studied. Therefore, we examined the efficacy of pruning at three different times of the year in commercial orchards in 2018 and 2019. We evaluated the efficacy of pruning for longer-term sustainable management of A. mali in wild apple forests over a five-year period from 2016–2019. Autumn pruning resulted in mortality of all immature A. mali in pruned branches, increased flowering in individual trees, and a significant reduction in both pest density and tree damage on the landscape. Simulation modeling showed that a single well-timed pruning treatment could maintain low pest density for at least 6–10 years. Recurring pruning schedules may be an effective long-term management tool, suppressing A. mali populations in wild apple forests and facilitating ecosystem recovery. This practical tactic could prove instrumental in managing A. mali and sustaining ecosystem health, particularly in the face of future invasions in wild apple forests in central Eurasia.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141567390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of aphid and ladybird to lead transfer through soil and broad beans 蚜虫和瓢虫对铅通过土壤和蚕豆转移的反应

IF 6.9 1区农林科学

Entomologia Generalis Pub Date : 2024-07-04 DOI: 10.1127/entomologia/2023/2277

Yan Li, Shasha Wang, Huiru Si, Sijing Wan, Guoyong Li, Yinghua Shu, Xiaoyan Dai, Ruijuan Wang, Shigui Wang, Yifan Zhai, Can Li, Bin Tang

{"title":"Responses of aphid and ladybird to lead transfer through soil and broad beans","authors":"Yan Li, Shasha Wang, Huiru Si, Sijing Wan, Guoyong Li, Yinghua Shu, Xiaoyan Dai, Ruijuan Wang, Shigui Wang, Yifan Zhai, Can Li, Bin Tang","doi":"10.1127/entomologia/2023/2277","DOIUrl":"https://doi.org/10.1127/entomologia/2023/2277","url":null,"abstract":"The impact of heavy metals on agricultural ecosystems has consistently been a topic of social concern. This study investigated the translocation of lead (Pb) along the soil – Vicia faba L. (Fabales: Fabaceae) – Megoura crassicauda (Hemiptera: Aphidoidea) – Harmonia axyridis (Coleoptera: Coccinellidae) system. Lead in soil could be transferred to broad bean, and the accumulation amount was highest in roots, followed by stems and leaves. Aphids and ladybirds could also accumulate lead through the food chain. Interestingly, the lead content of broad bean roots in the aphid feeding group was significantly higher than that in the non-aphid feeding group. Lead stress significantly reduced the germination rate and seedling height of broad bean, and inhibited root elongation. The expression levels of trehalase (TRE), trehalose-6-phosphate synthase (TPS), and vitellogenin (Vg), TRE activity, and carbohydrate content in aphids changed under lead treatment. The number of offspring produced by the second and fifth generation aphids was significantly reduced under lead treatment. Furthermore, high concentrations of lead treatment can prolong the development time of the 2nd and 3rd instar larvae of the ladybird. Feeding on aphids contaminated with lead can affect the survival of ladybirds. Our results further confirm the biological transfer of lead in the food chain and explore the adaptive mechanisms of aphids and ladybirds. These relevant results provide a theoretical basis for further exploring the molecular mechanism of lead homeostasis in plants and insects under lead stress.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141567275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-mite measures of aphids for protecting ants promote persistence of ant-aphid mutualism 蚜虫为保护蚂蚁而采取的防螨措施促进了蚂蚁-蚜虫互生关系的持续存在

IF 6.9 1区农林科学

Entomologia Generalis Pub Date : 2024-07-04 DOI: 10.1127/entomologia/2024/2601

Pengxiang Wu, Megan L. Head, Juan Yang, Yanan Wang, Qiaoling Lin

{"title":"Anti-mite measures of aphids for protecting ants promote persistence of ant-aphid mutualism","authors":"Pengxiang Wu, Megan L. Head, Juan Yang, Yanan Wang, Qiaoling Lin","doi":"10.1127/entomologia/2024/2601","DOIUrl":"https://doi.org/10.1127/entomologia/2024/2601","url":null,"abstract":"Numerous studies focus on food-for-protection ant-aphid mutualism while ignoring the potential aphid’s protection for ants. To obtain a comprehensive understanding of bidirectional services in ant-aphid mutualism in goji berry, we manipulated the presence and abundance of ladybugs or mites, analyzing their impact on the ant-aphid mutualism persistence. We found that ants provided aphid protection from ladybugs and aphids provided anti-mite measures for protecting ants. For aphid protection by ants, 1) ant presence eroded the positive aphid-ladybug abundance relationship by reducing ladybug attendance. 2) Aphid-infested plants with more ants were less acceptable to ladybugs (ant-ladybug ratio above 1.75). 3) Ant presence impacted consumption traits of ladybugs, including aphid consumption (43.5% less), handling time (220.3% more), and theoretical maximum consumption (68.8% less). For ant protection by aphids, 4) aphid presence alleviated negative impacts on ants caused by mites by reducing mite abundance. 5) High-abundance mites (mite-ant ratio above 70) significantly reduced ant attendance. 6) The substantial volume of aphid honeydew had remarkable anti-mite effects by trapping mites (above 0.7 ml per leaf). Besides food-for-protection mutualism, our study indicates that anti-mite measures of aphids for protecting ants could promote the persistence of ant-aphid mutualism. Both protections were density-dependent, suggesting in practical applications of ladybugs, inundative biological control based on density advantage could be a promising option for rapid impact on aphids. Effective control strategies should focus on reducing the aphid abundance below the economic threshold rather than attempting eradication, allowing aphids to naturally suppress mite populations. This could effectively reduce the dosage of pesticide products.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141567388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing RNAi feasibility and susceptibility to environmental RNAi in Trichogramma dendrolimi 评估 RNAi 的可行性以及树枝状毛虫对环境 RNAi 的易感性

IF 6.9 1区农林科学

Entomologia Generalis Pub Date : 2024-06-26 DOI: 10.1127/entomologia/2023/2210

Zhi-chao Yan, Fang-yi Li, Ao-kai Wang, Cheng-xing Wang, Hai-yan Wang, Ze-qi Yu, Ke-peng Wang, Yi-han Wang, Yuan-yuan Luo, Yuan-xi Li

{"title":"Assessing RNAi feasibility and susceptibility to environmental RNAi in Trichogramma dendrolimi","authors":"Zhi-chao Yan, Fang-yi Li, Ao-kai Wang, Cheng-xing Wang, Hai-yan Wang, Ze-qi Yu, Ke-peng Wang, Yi-han Wang, Yuan-yuan Luo, Yuan-xi Li","doi":"10.1127/entomologia/2023/2210","DOIUrl":"https://doi.org/10.1127/entomologia/2023/2210","url":null,"abstract":"Abstract: Trichogramma, a genus of egg parasitoid wasps, are widely used as biological control agents and serve as model organisms in parasitoid research. Despite their significance, the understanding of RNA interference (RNAi) in Trichogramma remains very limited. We identified RNAi-associated genes by bioinformatic approaches and experimentally assessed the feasibility of gene knockdown via RNAi for gene functional studies, as well as the susceptibility of T. dendrolimi to envionmental RNAi (dsRNAs targeting 12 genes ). We found that Trichogramma genomes contain a complete set of genes in the RNAi pathway and exhibit extensive gene expansion of dsRNase, which may influence RNAi efficiency by degrading dsRNA. We demonstrated successful RNAi through pupal microinjection in T. dendrolimi Matsumura, providing a technical approach for future gene functional studies. In addition, we observed no evidence of susceptibility to environmental RNAi in either T. dendrolimi adults or larvae. This low environmental RNAi sensitivity in Trichogramma could suggest a reduced risk of RNAi-based pest management strategies affecting nontarget Trichogramma populations. Overall, this study presents a technical approach for conducting gene functional studies in Trichogramma and provides a foundation for evaluating the nontarget effects of RNAi-based pest control strategies on Trichogramma.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141507621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imidazole-modified graphene quantum dots can effectively promote the efficient silencing of the larval cuticle protein gene HaLCP17 in Helicoverpa armigera 咪唑修饰的石墨烯量子点可有效促进 Helicoverpa armigera 幼虫角质层蛋白基因 HaLCP17 的高效沉默

IF 6.9 1区农林科学

Entomologia Generalis Pub Date : 2024-06-26 DOI: 10.1127/entomologia/2024/2458

Meng-Ke Zhang, Fei-Feng Wang, Peng Qin, Jie Chen, Yuan-Yuan Huang, Lin Yu, Jian-Yu Meng, Wen Sang

{"title":"Imidazole-modified graphene quantum dots can effectively promote the efficient silencing of the larval cuticle protein gene HaLCP17 in Helicoverpa armigera","authors":"Meng-Ke Zhang, Fei-Feng Wang, Peng Qin, Jie Chen, Yuan-Yuan Huang, Lin Yu, Jian-Yu Meng, Wen Sang","doi":"10.1127/entomologia/2024/2458","DOIUrl":"https://doi.org/10.1127/entomologia/2024/2458","url":null,"abstract":"RNA interference (RNAi) is a pivotal strategy in gene functional analysis and pest management. However, the efficacy of RNAi varies significantly across different insect species, particularly exhibiting limited effectiveness in Lepidopteran insects. Therefore, it is imperative to develop innovative delivery strategies of dsRNA to enhance efficient gene silencing in these insects. In this study, we utilized the globally distributed polyphagous Lepidopteran insect pest Helicoverpa armigera as our research model due to its substantial implications for agriculture and limited success with RNAi-based approaches. Through the utilization of RNAi technique, HaLCP17 was identified as a larval cuticle protein gene with the highest lethality among 61 potential lethal target genes of H. armigera. Additionally, silencing HaLCP17 during the larval stage resulted in a maximum decrease of 2.66-fold in expression, accompanied by a mortality rate of 23.33% after 10 days. The larvae in the treatment group exhibited significant reductions in size and epidermal relaxation, leading to maximum decrease of 28.89% and 53.57% in feeding amount and larval weight respectively. Notably, the introduction of Imidazole-Modified Graphene Quantum Dots (IGQDs), a novel type of quantum dot nanoparticles, into the insect RNAi system demonstrated proficient delivery capability for dsRNA and potentiated gene silencing efficiency. Compared with naked dsRNA treatment, the injection of IGQDs-dsHaLCP17 complex reduced the expression of HaLCP17 by 1.66-fold, on the 10th day after treatment the mortality rate increased by 92.88%, and the eclosion rate decreased by 31.42%. These findings hold great potential for future control and analysis of gene function in Lepidopteran insects.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141507641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multigenerational responses of wheat aphids to multiple stresses: interactions between sublethal thermal stress types and a low insecticide dose 小麦蚜虫对多种胁迫的多代反应：亚致死热胁迫类型与低剂量杀虫剂之间的相互作用

IF 6.9 1区农林科学

Entomologia Generalis Pub Date : 2024-06-26 DOI: 10.1127/entomologia/2023/2300

Kun Xing, Ze-Fang Mo, Zhi-Ping Xu, Jia-jia Wei, Hui Cheng, Fei Zhao

{"title":"Multigenerational responses of wheat aphids to multiple stresses: interactions between sublethal thermal stress types and a low insecticide dose","authors":"Kun Xing, Ze-Fang Mo, Zhi-Ping Xu, Jia-jia Wei, Hui Cheng, Fei Zhao","doi":"10.1127/entomologia/2023/2300","DOIUrl":"https://doi.org/10.1127/entomologia/2023/2300","url":null,"abstract":"The interaction between thermal stress and chemical insecticides in agricultural pests has become more common owing to the global warming and human activities. Although research has shown the interaction of constant temperature or single thermal stress with insecticides, the ecological effects of sublethal thermal stress and low-dose insecticide interaction, which are more applicate to field conditions, are limited. Therefore, this study investigates the interaction of sublethal thermal stress (TS) (34 °C/180 min, 36 °C/30 min, and 38 °C/10 min) and low-dose imidacloprid (IM) applications in the maternal and offspring phenotype of the important grain aphid, Sitobion avenae (Fabricius) (Hemiptera: Aphididae). In the F0 generation, single TS significantly reduced the longevity, fecundity, survivorship, and population parameters of S. avenae, and the interaction between TS and IM (TS-IM) had a significant negative effect on the intrinsic rate of increase in the order 34 °C/180 min + IM > 36 °C/30 min + IM > 38 °C/10 min + IM. In the F1 generation, the development duration of nymph was increased and the intrinsic rate of increase was reduced after F0 underwent TS. However, the intrinsic rate of increase of F1 generation showed the greatest decrease under 38 °C/10 min + IM, and the lowest decrease under 34 °C/180 min + IM. The results of this study reveal the significance of evaluating together different abiotic factors, such as the interaction between thermal stress and insecticides, in the risk assessment of climate change, emphasizing the importance of the combination of different stresses in pest control.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141507636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-vitro-expressed pxy-mir-34 shows high toxicity to multiple lepidopteran pests 体外表达的 pxy-mir-34 对多种鳞翅目害虫具有高毒性

IF 6.9 1区农林科学

Entomologia Generalis Pub Date : 2024-06-24 DOI: 10.1127/entomologia/2024/2365

Linhong Li, Dandan Shi, Juncheng Yan, Bin Zhu, Pei Liang

{"title":"In-vitro-expressed pxy-mir-34 shows high toxicity to multiple lepidopteran pests","authors":"Linhong Li, Dandan Shi, Juncheng Yan, Bin Zhu, Pei Liang","doi":"10.1127/entomologia/2024/2365","DOIUrl":"https://doi.org/10.1127/entomologia/2024/2365","url":null,"abstract":"Lepidopteran pests cause enormous economic losses to agriculture globally. The control of these pests relies heavily on chemical insecticides, resulting in severe insecticide resistance and food safety concerns. Therefore, there is an urgent need to develop alternative green strategies. Our previous research revealed that the miRNA of the diamondback moth Plutella xylostella, pxy-miR-34-5p, may be a novel molecular target for multiple lepidopteran pests. However, the application strategy for pxy-miR-34-5p is not yet clear. In this study, pxy-mir-34 was successfully expressed in Escherichia coli to produce artificial miR-34 (amiR-34) on a large scale. Treatment with amiR-34 significantly repressed the expression of the ecdysone receptor (EcR) and 12 other key genes involved in the growth and development of P. xylostella. Feeding bioassays showed that amiR-34 was highly toxic to multiple lepidopteran pests, and pot experiments demonstrated that spraying with amiR-34 led to larval mortalities of 81.7%, 89.3%, and 90.8% for P. xylostella, Spodoptera frugiperda, and Spodoptera exigua, respectively, with a persistence of up to five d. These results indicate that amiR-34 has great potential for controlling multiple lepidopterous pests and they lay an important foundation for developing miRNA-based green pest control strategies.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141507637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spinetoram exposure disrupts survival of flower bugs and leads to population collapse in orchards 暴露于辛喷罗姆会破坏花虫的生存，导致果园中的花虫数量崩溃

IF 6.9 1区农林科学

Entomologia Generalis Pub Date : 2024-06-19 DOI: 10.1127/entomologia/2023/2350

Tao Lin, Yong Chen, Yixin Chen, Jinfeng Hu, Shuo Lin, Jianwei Zhao, Guang Yang, Fenghua Yang, Hui Wei

{"title":"Spinetoram exposure disrupts survival of flower bugs and leads to population collapse in orchards","authors":"Tao Lin, Yong Chen, Yixin Chen, Jinfeng Hu, Shuo Lin, Jianwei Zhao, Guang Yang, Fenghua Yang, Hui Wei","doi":"10.1127/entomologia/2023/2350","DOIUrl":"https://doi.org/10.1127/entomologia/2023/2350","url":null,"abstract":"The increasing use of reduced-risk insecticides in orchard management has raised concerns regarding their potential risk to beneficial arthropods. Depending solely on acute short-term effects tends to underestimate the risks posed by spinetoram to natural enemies. Limited information is available on the long-term consequences of low exposure to spinetoram on natural enemies. The survival and predation of Orius strigicollis gradually decreased with the increase in spinetoram exposure rate, and the preoviposition periods were prolonged above 0.208 g a.i. ha-1. However, the adult lifespan, reproductive rate, population parameters, intrinsic rate of increase, finite rate of increase, and net reproductive rate were reduced, while the transformation rate was increased with the increase in spinetoram exposure rate. The population size and predation potential of O. strigicollis at 0.104 g a.i. ha-1 were higher than other treatments but did not differ from those of the control. Field residue monitoring showed that the peak residue levels of spinetoram on Eriobotrya japonica and Bidens alba flowers were 0.525–0.589 g ha-1 and 0.139–0.148 g ha-1, respectively, and it required at least 28 and 9 days, respectively, for the residue levels to dissipate to a level with no toxic effect on O. strigicollis. Therefore, the sublethal effects resulting from trace residues is the primary factor responsible for O. strigicollis population collapse. We provide insights for mitigating the negative impacts of spinetoram on O. strigicollis and for integrated pest management development.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":6.9,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141507640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0