Imidazole-modified graphene quantum dots can effectively promote the efficient silencing of the larval cuticle protein gene HaLCP17 in Helicoverpa armigera
Meng-Ke Zhang, Fei-Feng Wang, Peng Qin, Jie Chen, Yuan-Yuan Huang, Lin Yu, Jian-Yu Meng, Wen Sang
{"title":"Imidazole-modified graphene quantum dots can effectively promote the efficient silencing of the larval cuticle protein gene HaLCP17 in Helicoverpa armigera","authors":"Meng-Ke Zhang, Fei-Feng Wang, Peng Qin, Jie Chen, Yuan-Yuan Huang, Lin Yu, Jian-Yu Meng, Wen Sang","doi":"10.1127/entomologia/2024/2458","DOIUrl":null,"url":null,"abstract":"RNA interference (RNAi) is a pivotal strategy in gene functional analysis and pest management. However, the efficacy of RNAi varies significantly across different insect species, particularly exhibiting limited effectiveness in Lepidopteran insects. Therefore, it is imperative to develop innovative delivery strategies of dsRNA to enhance efficient gene silencing in these insects. In this study, we utilized the globally distributed polyphagous Lepidopteran insect pest Helicoverpa armigera as our research model due to its substantial implications for agriculture and limited success with RNAi-based approaches. Through the utilization of RNAi technique, HaLCP17 was identified as a larval cuticle protein gene with the highest lethality among 61 potential lethal target genes of H. armigera. Additionally, silencing HaLCP17 during the larval stage resulted in a maximum decrease of 2.66-fold in expression, accompanied by a mortality rate of 23.33% after 10 days. The larvae in the treatment group exhibited significant reductions in size and epidermal relaxation, leading to maximum decrease of 28.89% and 53.57% in feeding amount and larval weight respectively. Notably, the introduction of Imidazole-Modified Graphene Quantum Dots (IGQDs), a novel type of quantum dot nanoparticles, into the insect RNAi system demonstrated proficient delivery capability for dsRNA and potentiated gene silencing efficiency. Compared with naked dsRNA treatment, the injection of IGQDs-dsHaLCP17 complex reduced the expression of HaLCP17 by 1.66-fold, on the 10th day after treatment the mortality rate increased by 92.88%, and the eclosion rate decreased by 31.42%. These findings hold great potential for future control and analysis of gene function in Lepidopteran insects.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomologia Generalis","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1127/entomologia/2024/2458","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
RNA interference (RNAi) is a pivotal strategy in gene functional analysis and pest management. However, the efficacy of RNAi varies significantly across different insect species, particularly exhibiting limited effectiveness in Lepidopteran insects. Therefore, it is imperative to develop innovative delivery strategies of dsRNA to enhance efficient gene silencing in these insects. In this study, we utilized the globally distributed polyphagous Lepidopteran insect pest Helicoverpa armigera as our research model due to its substantial implications for agriculture and limited success with RNAi-based approaches. Through the utilization of RNAi technique, HaLCP17 was identified as a larval cuticle protein gene with the highest lethality among 61 potential lethal target genes of H. armigera. Additionally, silencing HaLCP17 during the larval stage resulted in a maximum decrease of 2.66-fold in expression, accompanied by a mortality rate of 23.33% after 10 days. The larvae in the treatment group exhibited significant reductions in size and epidermal relaxation, leading to maximum decrease of 28.89% and 53.57% in feeding amount and larval weight respectively. Notably, the introduction of Imidazole-Modified Graphene Quantum Dots (IGQDs), a novel type of quantum dot nanoparticles, into the insect RNAi system demonstrated proficient delivery capability for dsRNA and potentiated gene silencing efficiency. Compared with naked dsRNA treatment, the injection of IGQDs-dsHaLCP17 complex reduced the expression of HaLCP17 by 1.66-fold, on the 10th day after treatment the mortality rate increased by 92.88%, and the eclosion rate decreased by 31.42%. These findings hold great potential for future control and analysis of gene function in Lepidopteran insects.
期刊介绍:
Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology.
Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods.
Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.