Imidazole-modified graphene quantum dots can effectively promote the efficient silencing of the larval cuticle protein gene HaLCP17 in Helicoverpa armigera

IF 5.6 1区农林科学 Q1 ENTOMOLOGY

Entomologia Generalis Pub Date : 2024-06-26 DOI:10.1127/entomologia/2024/2458

Meng-Ke Zhang, Fei-Feng Wang, Peng Qin, Jie Chen, Yuan-Yuan Huang, Lin Yu, Jian-Yu Meng, Wen Sang

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Abstract

RNA interference (RNAi) is a pivotal strategy in gene functional analysis and pest management. However, the efficacy of RNAi varies significantly across different insect species, particularly exhibiting limited effectiveness in Lepidopteran insects. Therefore, it is imperative to develop innovative delivery strategies of dsRNA to enhance efficient gene silencing in these insects. In this study, we utilized the globally distributed polyphagous Lepidopteran insect pest Helicoverpa armigera as our research model due to its substantial implications for agriculture and limited success with RNAi-based approaches. Through the utilization of RNAi technique, HaLCP17 was identified as a larval cuticle protein gene with the highest lethality among 61 potential lethal target genes of H. armigera. Additionally, silencing HaLCP17 during the larval stage resulted in a maximum decrease of 2.66-fold in expression, accompanied by a mortality rate of 23.33% after 10 days. The larvae in the treatment group exhibited significant reductions in size and epidermal relaxation, leading to maximum decrease of 28.89% and 53.57% in feeding amount and larval weight respectively. Notably, the introduction of Imidazole-Modified Graphene Quantum Dots (IGQDs), a novel type of quantum dot nanoparticles, into the insect RNAi system demonstrated proficient delivery capability for dsRNA and potentiated gene silencing efficiency. Compared with naked dsRNA treatment, the injection of IGQDs-dsHaLCP17 complex reduced the expression of HaLCP17 by 1.66-fold, on the 10th day after treatment the mortality rate increased by 92.88%, and the eclosion rate decreased by 31.42%. These findings hold great potential for future control and analysis of gene function in Lepidopteran insects.

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咪唑修饰的石墨烯量子点可有效促进 Helicoverpa armigera 幼虫角质层蛋白基因 HaLCP17 的高效沉默

RNA 干扰（RNAi）是基因功能分析和害虫管理的重要策略。然而，RNAi 在不同昆虫物种中的效力差异很大，尤其是在鳞翅目昆虫中的效力有限。因此，当务之急是开发创新的 dsRNA 递送策略，以提高这些昆虫的基因沉默效率。在本研究中，我们利用了分布于全球的多食性鳞翅目昆虫害虫 Helicoverpa armigera 作为研究模型，因为它对农业有重大影响，而基于 RNAi 的方法效果有限。通过利用 RNAi 技术，我们发现 HaLCP17 是 H. armigera 61 个潜在致死靶基因中致死率最高的幼虫角质层蛋白基因。此外，在幼虫期沉默 HaLCP17 可使其表达量减少 2.66 倍，10 天后死亡率为 23.33%。处理组的幼虫体型明显缩小，表皮松弛，导致摄食量和幼虫体重最大分别减少 28.89% 和 53.57%。值得注意的是，在昆虫 RNAi 系统中引入咪唑修饰石墨烯量子点（IGQDs）这一新型量子点纳米粒子后，dsRNA 的输送能力得到了充分展示，基因沉默效率也得到了提高。与裸dsRNA处理相比，注射IGQDs-dsHaLCP17复合物后，HaLCP17的表达量减少了1.66倍，处理后第10天死亡率增加了92.88%，羽化率降低了31.42%。这些发现为今后控制和分析鳞翅目昆虫的基因功能提供了巨大的潜力。

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来源期刊

Entomologia Generalis 生物-昆虫学

CiteScore

7.10

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology. Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods. Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.