Assessing RNAi feasibility and susceptibility to environmental RNAi in Trichogramma dendrolimi

IF 5.6 1区农林科学 Q1 ENTOMOLOGY

Entomologia Generalis Pub Date : 2024-06-26 DOI:10.1127/entomologia/2023/2210

Zhi-chao Yan, Fang-yi Li, Ao-kai Wang, Cheng-xing Wang, Hai-yan Wang, Ze-qi Yu, Ke-peng Wang, Yi-han Wang, Yuan-yuan Luo, Yuan-xi Li

{"title":"Assessing RNAi feasibility and susceptibility to environmental RNAi in Trichogramma dendrolimi","authors":"Zhi-chao Yan, Fang-yi Li, Ao-kai Wang, Cheng-xing Wang, Hai-yan Wang, Ze-qi Yu, Ke-peng Wang, Yi-han Wang, Yuan-yuan Luo, Yuan-xi Li","doi":"10.1127/entomologia/2023/2210","DOIUrl":null,"url":null,"abstract":"Abstract: Trichogramma, a genus of egg parasitoid wasps, are widely used as biological control agents and serve as model organisms in parasitoid research. Despite their significance, the understanding of RNA interference (RNAi) in Trichogramma remains very limited. We identified RNAi-associated genes by bioinformatic approaches and experimentally assessed the feasibility of gene knockdown via RNAi for gene functional studies, as well as the susceptibility of T. dendrolimi to envionmental RNAi (dsRNAs targeting 12 genes ). We found that Trichogramma genomes contain a complete set of genes in the RNAi pathway and exhibit extensive gene expansion of dsRNase, which may influence RNAi efficiency by degrading dsRNA. We demonstrated successful RNAi through pupal microinjection in T. dendrolimi Matsumura, providing a technical approach for future gene functional studies. In addition, we observed no evidence of susceptibility to environmental RNAi in either T. dendrolimi adults or larvae. This low environmental RNAi sensitivity in Trichogramma could suggest a reduced risk of RNAi-based pest management strategies affecting nontarget Trichogramma populations. Overall, this study presents a technical approach for conducting gene functional studies in Trichogramma and provides a foundation for evaluating the nontarget effects of RNAi-based pest control strategies on Trichogramma.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomologia Generalis","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1127/entomologia/2023/2210","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract: Trichogramma, a genus of egg parasitoid wasps, are widely used as biological control agents and serve as model organisms in parasitoid research. Despite their significance, the understanding of RNA interference (RNAi) in Trichogramma remains very limited. We identified RNAi-associated genes by bioinformatic approaches and experimentally assessed the feasibility of gene knockdown via RNAi for gene functional studies, as well as the susceptibility of T. dendrolimi to envionmental RNAi (dsRNAs targeting 12 genes ). We found that Trichogramma genomes contain a complete set of genes in the RNAi pathway and exhibit extensive gene expansion of dsRNase, which may influence RNAi efficiency by degrading dsRNA. We demonstrated successful RNAi through pupal microinjection in T. dendrolimi Matsumura, providing a technical approach for future gene functional studies. In addition, we observed no evidence of susceptibility to environmental RNAi in either T. dendrolimi adults or larvae. This low environmental RNAi sensitivity in Trichogramma could suggest a reduced risk of RNAi-based pest management strategies affecting nontarget Trichogramma populations. Overall, this study presents a technical approach for conducting gene functional studies in Trichogramma and provides a foundation for evaluating the nontarget effects of RNAi-based pest control strategies on Trichogramma.

查看原文本刊更多论文

评估 RNAi 的可行性以及树枝状毛虫对环境 RNAi 的易感性

摘要：卵寄生蜂 Trichogramma 属被广泛用作生物控制剂，也是寄生虫研究中的模式生物。尽管其意义重大，但人们对三蛱蝶体内 RNA 干扰（RNAi）的了解仍然非常有限。我们通过生物信息学方法确定了 RNAi 相关基因，并通过实验评估了通过 RNAi 敲除基因进行基因功能研究的可行性，以及 T. dendrolimi 对环境 RNAi（针对 12 个基因的 dsRNA）的敏感性。我们发现，Trichogramma 基因组包含 RNAi 途径中的一整套基因，并表现出广泛的 dsRNase 基因扩增，这可能会通过降解 dsRNA 影响 RNAi 的效率。我们通过松村蝶蛹的显微注射成功地进行了 RNAi，为未来的基因功能研究提供了一种技术方法。此外，我们没有观察到 T. dendrolimi 成虫或幼虫对环境 RNAi 敏感的证据。这种对环境 RNAi 的低敏感性可能表明，基于 RNAi 的害虫管理策略会降低影响非目标 Trichogramma 种群的风险。总之，本研究提出了一种在毛地黄中进行基因功能研究的技术方法，并为评估基于 RNAi 的害虫控制策略对毛地黄的非目标影响奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Entomologia Generalis 生物-昆虫学

CiteScore

7.10

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology. Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods. Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.