Engineering in Life Sciences最新文献_第8页

Biological recovery of phosphorus (BioP-Rec) from wastewater streams using brewer's yeast on pilot-scale 利用酿酒酵母在中试规模上从废水中进行生物磷回收（BioP-Rec）

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-12-08 DOI: 10.1002/elsc.202300208

Vedran Vučić, Hauke Harms, Susann Müller

{"title":"Biological recovery of phosphorus (BioP-Rec) from wastewater streams using brewer's yeast on pilot-scale","authors":"Vedran Vučić, Hauke Harms, Susann Müller","doi":"10.1002/elsc.202300208","DOIUrl":"10.1002/elsc.202300208","url":null,"abstract":"Most recent advances for phosphorus (P) recovery using brewery yeast on laboratory scale were used to scale up to a pilot-scale process (BioP-Rec module) and applied in a full-scale wastewater treatment plant (WWTP). A P balance was established for WWTP Markranstädt according to two thresholds: (1) the economic feasibility threshold for P recovery of 0.05 kg/m3 of free P, and (2) the German Sewage Sludge Ordinance (GSSO) threshold, which demands that all WWTPs with a P content in dry matter (DM) of biosolids of 20 gP/kgDM or higher in the coming years must perform mandatory P recovery. In terms of defined thresholds, return and excess sludges were identified as the most feasible WWTP process streams for P recovery. In a 1 m3 BioP-Rec module a 3 stage process was established. From the P-rich water-phase of the return sludge produced in stage 1, which contained 0.051 kg/m3 of free P, 77.56% was taken up by P-depleted brewer's yeast Saccharomyces pastorianus in 3 h in stage 2. In stage 3, the yeast was concentrated in 1 h to produce yeast sludge as a fertilizer product. We demonstrated a novel pilot-scale process for the production of bio-based P-rich fertilizer.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300208","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138563763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification 研制一种用于即时DNA扩增的表面声波诱导微流体细胞裂解装置

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-12-06 DOI: 10.1002/elsc.202300230

Abbas Ali Husseini, Ali Mohammad Yazdani, Fatemeh Ghadiri, Alper Şişman

{"title":"Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification","authors":"Abbas Ali Husseini, Ali Mohammad Yazdani, Fatemeh Ghadiri, Alper Şişman","doi":"10.1002/elsc.202300230","DOIUrl":"10.1002/elsc.202300230","url":null,"abstract":"We developed a microchip device using surface acoustic waves (SAW) and sharp-edge glass microparticles to rapidly lyse low-level cell samples. This microchip features a 13-finger pair interdigital transducer (IDT) with a 30-degree focused angle, creating high-intensity acoustic beams converging 6 mm away at a 16 MHz frequency. Cell lysis is achieved through centrifugal forces acting on Candida albicans cells and glass particles within the focal area. To optimize this SAW-induced streaming, we conducted 42 pilot experiments, varying electrical power, droplet volume, glass particle size, concentration, and lysis time, resulting in optimal conditions: an electrical signal of 2.5 W, a 20 μL sample volume, glass particle size below 10 μm, concentration of 0.2 μg, and a 5-min lysis period. We successfully amplified DNA target fragments directly from the lysate, demonstrating an efficient microchip-based cell lysis method. When combined with an isothermal amplification technique, this technology holds promise for rapid point-of-care (POC) applications.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300230","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivated meat manufacturing: Technology, trends, and challenges 人造肉制造:技术、趋势和挑战

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-11-20 DOI: 10.1002/elsc.202300227

Marline Kirsch, Jordi Morales-Dalmau, Antonina Lavrentieva

{"title":"Cultivated meat manufacturing: Technology, trends, and challenges","authors":"Marline Kirsch, Jordi Morales-Dalmau, Antonina Lavrentieva","doi":"10.1002/elsc.202300227","DOIUrl":"10.1002/elsc.202300227","url":null,"abstract":"The growing world population, public awareness of animal welfare, environmental impacts and changes in meat consumption leads to the search for novel approaches to food production. Novel foods include products with a new or specifically modified molecular structure, foods made from microorganisms, fungi, algae or insects, as well as from animal cell or tissue cultures. The latter approach is known by various names: “clean meat”, “in vitro meat” and “cell-cultured” or “(cell-)cultivated meat”. Here, cells isolated from agronomically important species are expanded ex vivo to produce cell biomass used in unstructured meat or to grow and differentiate cells on scaffolds to produce structured meat analogues. Despite the fast-growing field and high financial interest from investors and governments, cultivated meat production still faces challenges ranging from cell source choice, affordable expansion, use of cruelty-free and food-grade media, regulatory issues and consumer acceptance. This overview discusses the above challenges and possible solutions and strategies in the production of cultivated meat. The review integrates multifaceted historical, social, and technological insights of the field, and provides both an engaging comprehensive introduction for general interested and a robust perspective for experts.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"23 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300227","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome dynamics and products profiles of biowaste fermentation under different organic loads and additives 不同有机负荷和添加剂条件下生物垃圾发酵的微生物组动态和产物概况

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-11-13 DOI: 10.1002/elsc.202300216

Xinyu Zhu, Ping Li, Feng Ju

{"title":"Microbiome dynamics and products profiles of biowaste fermentation under different organic loads and additives","authors":"Xinyu Zhu, Ping Li, Feng Ju","doi":"10.1002/elsc.202300216","DOIUrl":"10.1002/elsc.202300216","url":null,"abstract":"Biowaste fermentation is a promising technology for low-carbon print bioenergy and biochemical production. Although it is believed that the microbiome determines both the fermentation efficiency and the product profiles of biowastes, the explicit mechanisms of how microbial activity controls fermentation processes remained to be unexplored. The current study investigated the microbiome dynamics and fermentation product profiles of biowaste fermentation under different organic loads (5, 20, and 40 g-VS/L) and with additives that potentially modulate the fermentation process via methanogenesis inhibition (2-bromoethanesulfonate) or electron transfer promotion (i.e., reduced iron, magnetite iron, and activated carbon). The overall fermentation products yields were 440, 373 and 208 CH4-eq/g-VS for low-, medium- and high-load fermentation. For low- and medium-load fermentation, volatile fatty acids (VFAs) were first accumulated and were gradually converted to methane. For high-load fermentation, VFAs were the main fermentation products during the entire fermentation period, accounting for 62% of all products. 16S rRNA-based analyses showed that both 2-bromoethanesulfonate addition and increase of organic loads inhibited the activity of methanogens and promoted the activity of distinct VFA-producing bacterial microbiomes. Moreover, the addition of activated carbon promoted the activity of H2-producing Bacteroides, homoacetogenic Eubacteriaceae and methanogenic Methanosarcinaceae, whose activity dynamics during the fermentation led to changes in acetate and methane production. The current results unveiled mechanisms of microbiome activity dynamics shaping the biowaste fermentation product profiles and provided the fundamental basis for the development of microbiome-guided engineering approaches to modulate biowaste fermentation toward high-value product recovery.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136351820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flavonoids, gut microbiota, and host lipid metabolism 类黄酮、肠道微生物群和宿主脂质代谢

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-11-13 DOI: 10.1002/elsc.202300065

Miao Zhou, Jie Ma, Meng Kang, Wenjie Tang, Siting Xia, Jie Yin, Yulong Yin

引用次数: 0

Cover Picture: Engineering in Life Sciences 11'23 封面图片:Engineering in Life Sciences 11'23

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-11-08 DOI: 10.1002/elsc.202370111

引用次数: 0

Endospore production of Bacillus spp. for industrial use 工业用芽孢杆菌的孢子内生产

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-10-12 DOI: 10.1002/elsc.202300013

Riekje Biermann, Sascha Beutel

引用次数: 0

Gut microbiota and childhood malnutrition: Understanding the link and exploring therapeutic interventions 肠道微生物群与儿童营养不良：了解联系并探索治疗干预措施

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-10-05 DOI: 10.1002/elsc.202300070

Sevda Zoghi, Fatemah Sadeghpour Heravi, Zeinab Nikniaz, Masoud Shirmohamadi, Seyed Yaghoub Moaddab, Hamed Ebrahimzadeh Leylabadlo

{"title":"Gut microbiota and childhood malnutrition: Understanding the link and exploring therapeutic interventions","authors":"Sevda Zoghi, Fatemah Sadeghpour Heravi, Zeinab Nikniaz, Masoud Shirmohamadi, Seyed Yaghoub Moaddab, Hamed Ebrahimzadeh Leylabadlo","doi":"10.1002/elsc.202300070","DOIUrl":"10.1002/elsc.202300070","url":null,"abstract":"Childhood malnutrition is a metabolic condition that affects the physical and mental well-being of children and leads to resultant disorders in maturity. The development of childhood malnutrition is influenced by a number of physiological and environmental factors including metabolic stress, infections, diet, genetic variables, and gut microbiota. The imbalanced gut microbiota is one of the main environmental risk factors that significantly influence host physiology and childhood malnutrition progression. In this review, we have evaluated the gut microbiota association with undernutrition and overnutrition in children, and then the quantitative and qualitative significance of gut dysbiosis in order to reveal the impact of gut microbiota modification using probiotics, prebiotics, synbiotics, postbiotics, fecal microbiota transplantation, and engineering biology methods as new therapeutic challenges in the management of disturbed energy homeostasis. Understanding the host–microbiota interaction and the remote regulation of other organs and pathways by gut microbiota can improve the effectiveness of new therapeutic approaches and mitigate the negative consequences of childhood malnutrition.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135482137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Picture: Engineering in Life Sciences 10'23 封面图片：生命科学工程10’23

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-10-03 DOI: 10.1002/elsc.202370101

引用次数: 0

Bioprocess development for endospore production by Bacillus coagulans using an optimized chemically defined medium 使用优化的化学限定培养基由凝结芽孢杆菌生产内生孢子的生物工艺开发

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-09-15 DOI: 10.1002/elsc.202300210

Riekje Biermann, Laura Rösner, Lisa-Marie Beyer, Laura Niemeyer, Sascha Beutel

{"title":"Bioprocess development for endospore production by Bacillus coagulans using an optimized chemically defined medium","authors":"Riekje Biermann, Laura Rösner, Lisa-Marie Beyer, Laura Niemeyer, Sascha Beutel","doi":"10.1002/elsc.202300210","DOIUrl":"https://doi.org/10.1002/elsc.202300210","url":null,"abstract":"Bacillus coagulans is a promising probiotic, because it combines probiotic properties of Lactobacillus and the ability of Bacillus to form endospores. Due to this hybrid relationship, cultivation of this organism is challenging. As the probiotics market continues to grow, there is a new focus on the production of these microorganisms. In this work, a strain‐specific bioprocess for B. coagulans was developed to support growth on one hand and ensure sporulation on the other hand. This circumstance is not trivial, since these two metabolic states are contrary. The developed bioprocess uses a modified chemically defined medium which was further investigated in a one‐factor‐at‐a‐time assay after adaptation. A transfer from the shake flask to the bioreactor was successfully demonstrated in the scope of this work. The investigated process parameters included temperature, agitation and pH‐control. Especially the pH‐control improved the sporulation in the bioreactor when compared to shake flasks. The bioprocess resulted in a sporulation efficiency of 80%–90%. This corresponds to a sevenfold increase in sporulation efficiency due to a transfer to the bioreactor with pH‐control. Additionally, a design of experiment (DoE) was conducted to test the robustness of the bioprocess. This experiment validated the beforementioned sporulation efficiency for the developed bioprocess. Afterwards the bioprocess was then scaled up from a 1 L scale to a 10 L bioreactor scale. A comparable sporulation efficiency of 80% as in the small scale was achieved. The developed bioprocess facilitates the upscaling and application to an industrial scale, and can thus help meet the increasing market for probiotics.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"23 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50134360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0