Engineering in Life Sciences最新文献

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 9","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148105163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Raman-Chromatography Assembly for Automated Calibration and In-Line Monitoring in Bioprocessing","authors":"Jakob Heyer-Müller,&nbsp;Robin Schiemer,&nbsp;Matthias Lopinski,&nbsp;Caty Wang,&nbsp;Franka Willems,&nbsp;Lars Robbel,&nbsp;Michael Schmitt,&nbsp;Jurgen Hubbuch","doi":"10.1002/elsc.70044","DOIUrl":"10.1002/elsc.70044","url":null,"abstract":"<p>Optical spectroscopic techniques have been successfully employed in bioprocessing as process analytical technology for real-time process monitoring in numerous applications. The implementation of spectroscopy-based PAT techniques commonly necessitates the generation of representative process data used for calibration and validation of multivariate statistical models for analyzing the sample composition in real-time. To automate the generation of such data, we present a novel assembly of a commercially available chromatography system in combination with a Raman spectrometer for fast and accurate acquisition of Raman spectra. Using the ultra-/diafiltration (UF/DF) process as a case study, our methodology involved the preparation of representative calibration and validation mixtures of phosphate and citrate buffer and lysozyme as a model protein. Chemometric PLS models were calibrated and validated using these datasets, and applied to in-line recorded Raman spectra during a UF/DF experiment. The primary results demonstrated that the novel assembly provides robust and precise offline measurement of Raman spectra, which directly compare with in-line record data. The chemometric PLS models showed good alignment in calibration and validation datasets (R2 and Q2), and could be used to simultaneously monitor the buffer and protein concentrations in real-time during UF/DF. This study provides a simple, commercially available setup for automated acquisition of Raman spectra and demonstrates its straightforward application to bioprocess monitoring.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 9","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Synthesis and Evaluation of PCL/Chitosan/CQD-Fe Magnetic Nanocomposite for Wound Healing: Emphasis on Gene Expression","authors":"","doi":"10.1002/elsc.70040","DOIUrl":"10.1002/elsc.70040","url":null,"abstract":"<p><b>RETRACTION:</b> E.M. Abed, F. Yazdian, A.A. Sepahi, and B. Rasekh, “Synthesis and Evaluation of PCL/Chitosan/CQD-Fe Magnetic Nanocomposite for Wound Healing: Emphasis on Gene Expression,” <i>Engineering in Life Sciences</i> 25, no. 1 (2025): e202400038, https://doi.org/10.1002/elsc.202400038.</p><p>The above article, published online on 19 January 2025 in Wiley Online Library (http://onlinelibrary.wiley.com/), has been retracted by agreement between the journal Editor-in-Chief, Ralf Takors; and Wiley-VCH GmbH. Following an investigation by the publisher, the parties have concluded that this article was accepted solely on the basis of a compromised peer review process. Therefore, the article must be retracted.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioreactor Systems to Mass-Produce the Undervalued Crop, Celosia argentea, With High Nutrient Impact","authors":"Chandika Ramlall,&nbsp;Nisha Singh,&nbsp;Shakira Shaik","doi":"10.1002/elsc.70038","DOIUrl":"10.1002/elsc.70038","url":null,"abstract":"<p><i>Celosia argentea</i> is an undervalued crop that shows potential for production enhancement due to elevated leaf nutrient accumulative ability. By investigating propagation using various in vitro culture systems, thidiazuron (TDZ)-supplemented nutrient media enhanced yield from 10 plants per explant in semi-solid medium, to 27 under continuous immersion in liquid media in recipient for automated temporary immersion (RITA) bioreactors, to 63 under temporary immersion in liquid media in a balloon-type bubble bioreactor (BTBB). TDZ in the BTBB system also increased shoot biomass and subsequent nutrient content relative to TDZ-free media in ex vitro plants. Ex vitro plants originating from both continuous and temporary media immersion in BTBBs outperformed those in all other culture systems in accumulating leaf Mg, Fe, Ca and Zn to meet the recommended dietary allowance for males and females. The genotypic variance and genetic advance of the mean at 5% selection intensity varied for each nutrient per culture system, with and without TDZ. Selective breeding at 5% selection intensity would improve leaf nutrient content but is specific to the culture system and the presence of TDZ. This is the first study to use liquid-based bioreactor systems for <i>C. argentea</i> propagation thereby providing new opportunities to upscale plant production for high nutrient-accumulating genotypes.</p><p><i>Practical application</i>: This study establishes a commercially viable protocol for the large-scale clonal propagation of <i>Celosia argentea</i>, a nutrient-rich, fast growing leafy vegetable with untapped agronomic value. Using temporary immersion bioreactors and thidiazuron-supplemented media, the system delivers up to 63 plants per explant, more than 6-fold the yield of conventional methods, while significantly boosting leaf biomass and nutrient content (Mg, Ca, Fe, Zn). These results position <i>C. argentea</i> as a functional crop for health-focused markets and ready-to-cook vegetable lines. The low-input cultivation needs and rapid production cycle (8 weeks in vitro, 8 weeks ex vitro) make it ideal for high-turnover commercial nurseries, contract growers, and vertical farming operations. The systems reproducibility and high heritability of nutritional traits further support selective breeding programs for premium-value cultivars. This propagation platform offers agribusinesses a scalable entry point into the expanding market for nutrient-dense indigenous vegetables with health and wellness appeal.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 8","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Preparation of a pH-Responsive Chitosan-Montmorillonite-Nitrogen-Doped Carbon Quantum Dots Nanocarrier for Attenuating Doxorubicin Limitations in Cancer Therapy","authors":"","doi":"10.1002/elsc.70033","DOIUrl":"10.1002/elsc.70033","url":null,"abstract":"<p><b>RETRACTION</b>: E. Rahmani, M. Pourmadadi, S. A. Ghorbanian, F. Yazdian, H. Rashedi, and M. Navaee, “Preparation of a pH-Responsive Chitosan-Montmorillonite-Nitrogen-Doped Carbon Quantum Dots Nanocarrier for Attenuating Doxorubicin Limitations in Cancer Therapy,” <i>Engineering in Life Sciences</i> 22, no. 10 (2022): 634–649, https://doi.org/10.1002/elsc.202200016.</p><p>The above article, published online on 13 September 2022 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editors-in-Chief, Ralf Takors, An-Ping Zeng; and Wiley-VCH GmbH.</p><p>The retraction has been agreed upon following an investigation into concerns raised by a third party, which revealed:\u0000\u0000 </p><p>The partial raw data provided by the authors could not address the original concerns, showed inconsistencies with the published results, and ultimately raised additional doubts about the study's overall reliability. Consequently, the editors have lost confidence in the presented data and decided to retract the paper. The authors’ institute has been informed of the allegations and the decision to retract but remained unresponsive. The authors disagree with the retraction.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 7","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Cell Density Perfusion Process of Quail Cells Producing Oncolytic rVSV-NDV","authors":"Lennart Jacobtorweihe,&nbsp;Sven Göbel,&nbsp;Markus Wolschek,&nbsp;Jennifer Altomonte,&nbsp;Udo Reichl,&nbsp;Yvonne Genzel","doi":"10.1002/elsc.70035","DOIUrl":"10.1002/elsc.70035","url":null,"abstract":"<p>Oncolytic viruses as agents for the treatment of various types of cancer have demonstrated their potential in many clinical studies over the past decades. In particular, rVSV-NDV (a recombinant vesicular stomatitis virus [VSV] construct with fusogenic Newcastle disease virus glycoproteins) shows promising preclinical results. This is due to its safety profile, immunostimulatory effects, and efficacy based on strong syncytia formation. Since virotherapy requires a high input of infectious viruses, efficient production processes are needed. Good manufacturing practice (GMP)-compliant CCX.E10 cells have been previously reported as a high-titer-producing rVSV-NDV candidate in batch mode. Here, semi-perfusion was used to test quail-originated CCX.E10 cells for rVSV-NDV production at high cell densities and in different cell culture media. The best condition was transferred to a full perfusion process in a 3 L bioreactor using a tangential follow depth filtration (TFDF) device for cell retention. The integrated depth filter with a pore size of 2–5 µm allowed 99.9% cell retention at viable cell concentrations (VCCs) of up to 20.6 × 10⁶ cells/mL and continuous virus harvesting. With this setup, we were able to produce 1.33 × 10⁹ TCID₅₀/mL infectious virus with a 5-fold increase in space-time yield (STY) compared to a batch process as a control.</p><p><i>Practical application:</i> Despite significant progress in oncolytic virus development, early research primarily focuses on viral design and therapeutic potential, often overlooking production challenges until later stages. This gap hinders clinical translation, as manufacturing high oncolytic virus doses (up to 10¹¹ infectious particles per injection) remains a major bottleneck. Implementing GMP-compliant cell substrates alongside perfusion cultures is essential to overcoming the low yields of traditional batch production. These advancements have far-reaching implications for reducing costs, increasing dose availability, and accelerating the clinical adoption of this promising immunotherapy.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 7","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of Bioproducts from Wastewater Treatment Using the Microalga Neochloris oleoabundans","authors":"Fahd Mnasser,&nbsp;Mª Lourdes Martínez-Cartas,&nbsp;Sebastián Sánchez","doi":"10.1002/elsc.70032","DOIUrl":"10.1002/elsc.70032","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;p&gt;Olive mill wastewater (OMW) poses an environmental risk due to its high chemical oxygen demand (COD). It is also rich in nutrients and organic carbon and has been used in this study together with urban wastewater (UWW) as part of the &lt;i&gt;Neochloris oleoabundans&lt;/i&gt; culture medium in different proportions to analyze the results in the change of proportion in the mixtures, looking for the highest level of wastewater utilization. The objective was to determine both the growth capacity of the microorganism in this medium for subsequent production of bioproducts (such as pigments, crude protein, or lipids) from the generated microalgal biomass, as well as the rate of removal of compounds present in wastewater after treatment, which enables it to be discharged into public canals and used for irrigation. The bioprocesses which were developed in batch photobioreactors of 1 dm&lt;sup&gt;3&lt;/sup&gt;, showed the highest values of maximum specific growth rate and volumetric biomass productivity 0.082 h&lt;sup&gt;−1&lt;/sup&gt; and 3 mg dm&lt;sup&gt;−3&lt;/sup&gt; h&lt;sup&gt;−1&lt;/sup&gt;, respectively. The maximum percentage of crude proteins (49.5%), lipids (51.0%), and carbohydrates (56.0%) we obtained in the final biomass. In this regard, the biggest percentages of organic load removal in the treatment with &lt;i&gt;N. oleoabundans&lt;/i&gt; were 65.66% for total phenolic compounds and 93.7% for COD. In addition, the potential content of salts such as nitrates, nitrites, and orthophosphate decreased for 97.6%, 94.3%, and 69.2%, respectively. Values that show the suitability of &lt;i&gt;N. oleoabundans&lt;/i&gt; for the recovery of bioproducts and the reduction of the pollutant load of wastewater for its reuse in agriculture, increasing the circular bioeconomy in the olive sector.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Summary&lt;/h3&gt;\u0000 \u0000 &lt;div&gt;\u0000 &lt;ul&gt;\u0000 \u0000 &lt;li&gt;\u0000 &lt;p&gt;In a region with the highest olive oil production in the world, the high generation of waste from the olive oil industry requires the implementation of solutions to valorize and minimize the waste generated. The treatment of olive mill wastewater, used as a culture medium providing nutrients for the development of microalgae, is a promising procedure.&lt;/p&gt;\u0000 &lt;/li&gt;\u0000 \u0000 &lt;li&gt;\u0000 &lt;p&gt;In this study, the microalgae &lt;i&gt;Neochloris oleoabundans&lt;/i&gt; has been used to treat olive oil mill wastewater, which has allowed high yields to be obtained in the production of biomass that can be transformed into bioproducts such as carbohydrates, proteins, and lipids. In addition, a high yield is obtained in the elimination of components that can be harmful, which is relevant for the reuse of water for irrigation or for discharge into natural watercourses.&lt;/p&gt;\u0000 ","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 7","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144550830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Anion Exchange Membrane Adsorbers for Endotoxin Removal During Ultrafiltration and Diafiltration Buffer Preparation","authors":"Sven Heimann,&nbsp;Andrew Vail,&nbsp;Sophie Muczenski,&nbsp;Alexander Faude","doi":"10.1002/elsc.70029","DOIUrl":"10.1002/elsc.70029","url":null,"abstract":"<p>During the manufacturing of drug substances (DS), endotoxins are a commonly monitored contaminant and checked to ensure the safety and quality of the final product. In this study, we investigated endotoxin removal by commercial anion exchange membrane adsorbers during buffer preparation for an ultrafiltration and diafiltration (UFDF) unit operation as a risk mitigation strategy to meet DS endotoxin specifications. A bracketing design study was used to compare two quaternary amine (Q) modified membranes and one guanidinium functionalized hybrid adsorber for endotoxin removal from spiked buffers representing UFDF start and diafiltration buffer matrices. For the UFDF start buffer, Q-based membrane adsorbers were effective at the removal of endotoxin to the limit of detection in low ionic strength conditions, with one adsorber effective up to 24 mS/cm. Switching to a more complex UFDF diafiltration buffer, Q-based membrane adsorbers were impacted by additional buffer components. The guanidinium-based hybrid membrane adsorber demonstrated endotoxin reduction to the limit of detection from both buffer matrices, showing removal across a wide pH range (4.7–8.3) and conductivity as high as 43 mS/cm. These results demonstrate an operational window for buffers and selected membrane adsorbers to mitigate risk by limiting endotoxin contamination prior to UFDF operations in pharmaceutical applications.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 7","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144550889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148114752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PDMS Membrane Using Phenyl as Rigid Molecular Spacer for Phenol Recovery","authors":"Xiangyan Li,&nbsp;Yan Zhuang,&nbsp;Chang Liu,&nbsp;Chenlin Zhang,&nbsp;Chao Sang,&nbsp;Lankun Wang,&nbsp;Siyu Pang,&nbsp;Hanzhu Wu,&nbsp;Songyuan Yao,&nbsp;Sitong Li,&nbsp;Zhihao Si,&nbsp;Xinmiao Zhang,&nbsp;Peiyong Qin","doi":"10.1002/elsc.70030","DOIUrl":"10.1002/elsc.70030","url":null,"abstract":"<p>Polydimethylsiloxane (PDMS) is extensively utilized for the recovery of bio-alcohols, but it encounters significant obstacles in volatile organic compounds (VOCs) removal, because of the narrow size for molecules diffusion. In this work, we designed a high-efficiency diffusion channel by introducing phenyl as a spacer into PDMS chains. The monomer divinylbenzene and vinyl-terminated PDMS (vinyl-PDMS) can be chemically crosslinked with thiol-grafted PDMS (thiol-PDMS) based on thiol-ene click reaction. The result shows that the free volume radius (<i>r₃</i>, <i>r₄</i>) has a significant increase after the introduction of divinylbenzene as a spacer, which is beneficial to the transport of phenol diffusion. After a series of optimizations involving the divinylbenzene content, pervaporation (PV) operating temperature, photoinitiator content, and viscosity of vinyl-PDMS, the prepared phenyl-PDMS showed an excellent PV performance for phenol recovery containing 10.9 of separation factor and 3959.66 g m⁻² h⁻¹ of flux as separating 0.1 wt% of phenol/water solution at 70°C. This separation performance is significantly higher than the unmodified PDMS membrane, that is, 2.05 times higher in separation factor and 3.54 times higher in flux. This study provides an effective structure design for the removal of aromatic compounds by enlarging diffusion channels and will make a great contribution to biological medicine and bioengineering.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"25 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}