Engineering in Life Sciences最新文献_第7页

From single-cell cloning to high-yield influenza virus production – implementing advanced technologies in vaccine process development 从单细胞克隆到高产流感病毒生产--在疫苗工艺开发中采用先进技术

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2024-02-18 DOI: 10.1002/elsc.202300245

Tilia Zinnecker, Najd Badri, Diogo Araujo, Kristin Thiele, Udo Reichl, Yvonne Genzel

{"title":"From single-cell cloning to high-yield influenza virus production – implementing advanced technologies in vaccine process development","authors":"Tilia Zinnecker, Najd Badri, Diogo Araujo, Kristin Thiele, Udo Reichl, Yvonne Genzel","doi":"10.1002/elsc.202300245","DOIUrl":"10.1002/elsc.202300245","url":null,"abstract":"Innovations in viral vaccine manufacturing are crucial for pandemic preparedness and to meet ever-rising global demands. For influenza, however, production still mainly relies on technologies established decades ago. Although modern production shifts from egg-based towards cell culture technologies, the full potential has not yet been fully exploited. Here, we evaluate whether implementation of state-of-the-art technologies for cell culture-based recombinant protein production are capable to challenge outdated approaches in viral vaccine process development. For this, a fully automated single-cell cloning strategy was established to generate monoclonal suspension Madin-Darby canine kidney (MDCK) cells. Among selected cell clones, we could observe distinct metabolic and growth characteristics, with C59 reaching a maximum viable cell concentration of 17.3 × 106 cells/mL and low doubling times in batch mode. Screening for virus production using a panel of human vaccine-relevant influenza A and B viruses in an ambr15 system revealed high titers with yields competing or even outperforming available MDCK cell lines. With C113, we achieved cell-specific virus yields of up to 25,000 virions/cell, making this cell clone highly attractive for vaccine production. Finally, we confirmed process performance at a 50-fold higher working volume. In summary, we present a scalable and powerful approach for accelerated development of high-yield influenza virus production in chemically defined medium starting from a single cell.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300245","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139953422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Digitalization concepts in academic bioprocess development 学术生物工艺开发中的数字化概念

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2024-02-09 DOI: 10.1002/elsc.202300238

Tessa Habich, Sascha Beutel

{"title":"Digitalization concepts in academic bioprocess development","authors":"Tessa Habich, Sascha Beutel","doi":"10.1002/elsc.202300238","DOIUrl":"10.1002/elsc.202300238","url":null,"abstract":"Digitalization with integrated devices, digital and physical assistants, automation, and simulation is setting a new direction for laboratory work. Even with complex research workflows, high staff turnover, and a limited budget some laboratories have already shown that digitalization is indeed possible. However, academic bioprocess laboratories often struggle to follow the trend of digitalization. Due to their diverse research circumstances, high variety of team composition, goals, and limitations the concepts are substantially different. Here, we will provide an overview on different aspects of digitalization and describe how academic laboratories successfully digitalized their working environment. The key aspect is the collaboration and communication between IT-experts and scientific staff. The developed digital infrastructure is only useful if it supports the laboratory worker and does not complicate their work. Thereby, laboratory researchers have to collaborate closely with IT-experts in order for a well-developed and maintainable digitalization concept that fits their individual needs and level of complexity. This review may serve as a starting point or a collection of ideas for the transformation toward a digitalized laboratory.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300238","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139762068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Picture: Engineering in Life Sciences 2'24 封面图片：生命科学工程 2'24

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2024-02-05 DOI: 10.1002/elsc.202470021

引用次数: 0

Cover Picture: Engineering in Life Sciences 1'24 封面图片：生命科学工程 1'24

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2024-01-04 DOI: 10.1002/elsc.202470011

引用次数: 0

Automized inline monitoring in perfused mammalian cell culture by MIR spectroscopy without calibration model building 利用近红外光谱对灌注哺乳动物细胞培养进行自动在线监测，无需建立校准模型

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-12-11 DOI: 10.1002/elsc.202300237

Hannah Marienberg, Nicole Desch, Vitalii Mozin, Lorenz Sykora-Mirle, Anja Müller, Andreas Roth, Mathias Käfer, Rüdiger Neef

{"title":"Automized inline monitoring in perfused mammalian cell culture by MIR spectroscopy without calibration model building","authors":"Hannah Marienberg, Nicole Desch, Vitalii Mozin, Lorenz Sykora-Mirle, Anja Müller, Andreas Roth, Mathias Käfer, Rüdiger Neef","doi":"10.1002/elsc.202300237","DOIUrl":"10.1002/elsc.202300237","url":null,"abstract":"Process Analytical Technologies (PATs) are taking a key role in the run for automatization in the biopharmaceutical industry. Spectroscopic methods such as Raman spectroscopy or mid-infrared (MIR) spectroscopy are getting more recognition in the recent years for inline monitoring of bioprocesses due to their ability to measure various molecules simultaneously. However, their dependency on laborious model calibration making them a challenge to implement. In this study, a novel one-point calibration that requires a single reference point prior to the inline monitoring of glucose and lactate in bioprocesses with MIR spectroscopy is assessed with 22 mammalian cell perfusion (PER) processes in two different scales and four different products. Concentrations are predicted over all PERs runs with a root mean square error (RMSE) of 0.29 g/L for glucose and 0.24 g/L for lactate, respectively. For comparison conventional partial least square regression (PLSR) models were used and trained with spectroscopic data from six bioreactor runs in two different scales and three products. The general accuracy of those models (RMSE of 0.41 g/L for glucose and 0.16 g/L for lactate) are in the range of the accuracy of the one-point calibration. This shows the potential of the one-point calibration as an approach making spectroscopy more accessible for bioprocess development.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300237","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138629082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Picture: Engineering in Life Sciences 12'23 封面图片：生命科学工程 12'23

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-12-10 DOI: 10.1002/elsc.202370121

引用次数: 0

Biological recovery of phosphorus (BioP-Rec) from wastewater streams using brewer's yeast on pilot-scale 利用酿酒酵母在中试规模上从废水中进行生物磷回收（BioP-Rec）

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-12-08 DOI: 10.1002/elsc.202300208

Vedran Vučić, Hauke Harms, Susann Müller

{"title":"Biological recovery of phosphorus (BioP-Rec) from wastewater streams using brewer's yeast on pilot-scale","authors":"Vedran Vučić, Hauke Harms, Susann Müller","doi":"10.1002/elsc.202300208","DOIUrl":"10.1002/elsc.202300208","url":null,"abstract":"Most recent advances for phosphorus (P) recovery using brewery yeast on laboratory scale were used to scale up to a pilot-scale process (BioP-Rec module) and applied in a full-scale wastewater treatment plant (WWTP). A P balance was established for WWTP Markranstädt according to two thresholds: (1) the economic feasibility threshold for P recovery of 0.05 kg/m3 of free P, and (2) the German Sewage Sludge Ordinance (GSSO) threshold, which demands that all WWTPs with a P content in dry matter (DM) of biosolids of 20 gP/kgDM or higher in the coming years must perform mandatory P recovery. In terms of defined thresholds, return and excess sludges were identified as the most feasible WWTP process streams for P recovery. In a 1 m3 BioP-Rec module a 3 stage process was established. From the P-rich water-phase of the return sludge produced in stage 1, which contained 0.051 kg/m3 of free P, 77.56% was taken up by P-depleted brewer's yeast Saccharomyces pastorianus in 3 h in stage 2. In stage 3, the yeast was concentrated in 1 h to produce yeast sludge as a fertilizer product. We demonstrated a novel pilot-scale process for the production of bio-based P-rich fertilizer.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300208","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138563763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification 研制一种用于即时DNA扩增的表面声波诱导微流体细胞裂解装置

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-12-06 DOI: 10.1002/elsc.202300230

Abbas Ali Husseini, Ali Mohammad Yazdani, Fatemeh Ghadiri, Alper Şişman

{"title":"Developing a surface acoustic wave-induced microfluidic cell lysis device for point-of-care DNA amplification","authors":"Abbas Ali Husseini, Ali Mohammad Yazdani, Fatemeh Ghadiri, Alper Şişman","doi":"10.1002/elsc.202300230","DOIUrl":"10.1002/elsc.202300230","url":null,"abstract":"We developed a microchip device using surface acoustic waves (SAW) and sharp-edge glass microparticles to rapidly lyse low-level cell samples. This microchip features a 13-finger pair interdigital transducer (IDT) with a 30-degree focused angle, creating high-intensity acoustic beams converging 6 mm away at a 16 MHz frequency. Cell lysis is achieved through centrifugal forces acting on Candida albicans cells and glass particles within the focal area. To optimize this SAW-induced streaming, we conducted 42 pilot experiments, varying electrical power, droplet volume, glass particle size, concentration, and lysis time, resulting in optimal conditions: an electrical signal of 2.5 W, a 20 μL sample volume, glass particle size below 10 μm, concentration of 0.2 μg, and a 5-min lysis period. We successfully amplified DNA target fragments directly from the lysate, demonstrating an efficient microchip-based cell lysis method. When combined with an isothermal amplification technique, this technology holds promise for rapid point-of-care (POC) applications.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300230","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivated meat manufacturing: Technology, trends, and challenges 人造肉制造:技术、趋势和挑战

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-11-20 DOI: 10.1002/elsc.202300227

Marline Kirsch, Jordi Morales-Dalmau, Antonina Lavrentieva

{"title":"Cultivated meat manufacturing: Technology, trends, and challenges","authors":"Marline Kirsch, Jordi Morales-Dalmau, Antonina Lavrentieva","doi":"10.1002/elsc.202300227","DOIUrl":"10.1002/elsc.202300227","url":null,"abstract":"The growing world population, public awareness of animal welfare, environmental impacts and changes in meat consumption leads to the search for novel approaches to food production. Novel foods include products with a new or specifically modified molecular structure, foods made from microorganisms, fungi, algae or insects, as well as from animal cell or tissue cultures. The latter approach is known by various names: “clean meat”, “in vitro meat” and “cell-cultured” or “(cell-)cultivated meat”. Here, cells isolated from agronomically important species are expanded ex vivo to produce cell biomass used in unstructured meat or to grow and differentiate cells on scaffolds to produce structured meat analogues. Despite the fast-growing field and high financial interest from investors and governments, cultivated meat production still faces challenges ranging from cell source choice, affordable expansion, use of cruelty-free and food-grade media, regulatory issues and consumer acceptance. This overview discusses the above challenges and possible solutions and strategies in the production of cultivated meat. The review integrates multifaceted historical, social, and technological insights of the field, and provides both an engaging comprehensive introduction for general interested and a robust perspective for experts.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"23 12","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300227","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138507832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiome dynamics and products profiles of biowaste fermentation under different organic loads and additives 不同有机负荷和添加剂条件下生物垃圾发酵的微生物组动态和产物概况

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-11-13 DOI: 10.1002/elsc.202300216

Xinyu Zhu, Ping Li, Feng Ju

{"title":"Microbiome dynamics and products profiles of biowaste fermentation under different organic loads and additives","authors":"Xinyu Zhu, Ping Li, Feng Ju","doi":"10.1002/elsc.202300216","DOIUrl":"10.1002/elsc.202300216","url":null,"abstract":"Biowaste fermentation is a promising technology for low-carbon print bioenergy and biochemical production. Although it is believed that the microbiome determines both the fermentation efficiency and the product profiles of biowastes, the explicit mechanisms of how microbial activity controls fermentation processes remained to be unexplored. The current study investigated the microbiome dynamics and fermentation product profiles of biowaste fermentation under different organic loads (5, 20, and 40 g-VS/L) and with additives that potentially modulate the fermentation process via methanogenesis inhibition (2-bromoethanesulfonate) or electron transfer promotion (i.e., reduced iron, magnetite iron, and activated carbon). The overall fermentation products yields were 440, 373 and 208 CH4-eq/g-VS for low-, medium- and high-load fermentation. For low- and medium-load fermentation, volatile fatty acids (VFAs) were first accumulated and were gradually converted to methane. For high-load fermentation, VFAs were the main fermentation products during the entire fermentation period, accounting for 62% of all products. 16S rRNA-based analyses showed that both 2-bromoethanesulfonate addition and increase of organic loads inhibited the activity of methanogens and promoted the activity of distinct VFA-producing bacterial microbiomes. Moreover, the addition of activated carbon promoted the activity of H2-producing Bacteroides, homoacetogenic Eubacteriaceae and methanogenic Methanosarcinaceae, whose activity dynamics during the fermentation led to changes in acetate and methane production. The current results unveiled mechanisms of microbiome activity dynamics shaping the biowaste fermentation product profiles and provided the fundamental basis for the development of microbiome-guided engineering approaches to modulate biowaste fermentation toward high-value product recovery.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136351820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0