Engineering in Life Sciences最新文献

{"title":"Less Genome, More Gain: Genome Reduction Enhances Transaminase-Producing <i>E. coli</i> in a Scale-Down Bioreactor.","authors":"Gennaro Avolio, Simon Klaffl, Ralf Takors","doi":"10.1002/elsc.70080","DOIUrl":"https://doi.org/10.1002/elsc.70080","url":null,"abstract":"<p><p>In large-scale bioprocesses, mixing limitations and design constraints cause the onset of heterogeneous environments, subjecting the cells to continuously changing external conditions, often reducing their performance compared to laboratory conditions. This study evaluated the performance in producing a heterologous transaminase (TA) of a genome-reduced <i>Escherichia coli</i> strain (RM214) in a STR-PFR scale-down system, benchmarking it against a wild-type strain. Under cycles of glycerol limitation and starvation, combined with oxygen limitation in later process stages, RM214 outperformed the wild-type strain. Due to its lower maintenance coefficient, RM214 showed a remarkable biomass increase of +53% and a boosted final volumetric activity with a +65% increase. These results were achieved with significantly reduced biomass-specific substrate uptake rates and respiratory parameters, both crucial for optimizing large-scale processes. This study underscores the applicability and enhanced robustness of genome-reduced strains in heterogeneous large-scale environments.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"26 ","pages":"e70080"},"PeriodicalIF":3.0,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147766089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Filament Extrusion-Based Conductive TPU Composite Scaffolds Enable Superior Neuronal Growth and Synaptic Maturation In Vitro.","authors":"Kamil Elkhoury, Belal Shohayeb, Guan-Lin Chen, Erfan Noorbakhsh Noshahri, Julio Zuazola, Dan Ohtan Wang, Nikhil Gupta, Sanjairaj Vijayavenkataraman","doi":"10.1002/elsc.70078","DOIUrl":"https://doi.org/10.1002/elsc.70078","url":null,"abstract":"<p><p>Fused filament fabrication (FFF) three-dimensional (3D) printing technologies offer new opportunities for fabricating customizable, low-cost platforms for tissue engineering applications. Here, we developed and characterized 3D-printed scaffolds using conductive thermoplastic polyurethane (cTPU) filaments and evaluated their mechanical, electrical, and biological performance in vitro. Dynamic mechanical analysis (DMA) across a range of temperatures and frequencies revealed that both TPU and cTPU exhibit temperature- and rate-dependent elastic moduli, with cTPU showing enhanced mechanical stiffness due to the incorporation of conductive fillers. Electrical testing confirmed that cTPU exhibited a stable conductivity (∼1-2 mS/cm) resembling physiological conditions. Surface characterization showed that cTPU was significantly more hydrophilic and exhibited higher nanoscale roughness, both of which are favorable for cell-material interactions. Mouse embryonic fibroblasts (MEFs) cultured on both scaffolds showed high viability (>85%) and significant proliferation. Notably, immunofluorescence analysis of cultured hippocampal neurons revealed significantly higher density of neuronal networks represented by higher microtubule-associated protein 2 (MAP-2)-positive cell density, greater MAP-2 area coverage, larger average MAP-2 cell area, and enhanced postsynaptic density protein 95 (PSD-95) expression on cTPU scaffolds. Together, these results demonstrate that FFF 3D-printed cTPU platforms can support long-term neuronal growth and synaptic maturation, offering promising applications in neural tissue modeling and bioelectronic interfaces. <i>Practical Application:</i> Characterizing soft viscoelastic materials whose properties strongly depend on temperature and strain rate is challenging and typically requires extensive testing across multiple conditions. Using a single-specimen Dynamic Mechanical Analysis-based mechanical testing method and a viscoelastic-elastic transformation that converts frequency-domain viscoelastic measurements into elastic constants over a broad range of test conditions, validated by tensile tests, we efficiently generated reliable modulus data across a range of conditions, enhancing testing throughput without sacrificing accuracy. As a case study, we demonstrate the successful fabrication and comprehensive characterization of FDM 3D-printed conductive TPU (cTPU) scaffolds for potential applications in neural tissue modeling and bioelectronic interfaces, with the results positioning cTPU composites as cost-effective, tunable, cytocompatible, and electrically active platforms capable of supporting neuronal growth and function.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"26 ","pages":"e70078"},"PeriodicalIF":3.0,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13112004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147766061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Continuous Enzymatic Peracids Synthesis in Pickering Emulsions: Influence of Nanoparticles Modification, Aqueous Phase Composition and Operational Parameters","authors":"Lemuel Onoriode Adomi,&nbsp;Sara Fatima Bhutta,&nbsp;Marion B. Ansorge-Schumacher,&nbsp;Anja Drews","doi":"10.1002/elsc.70077","DOIUrl":"10.1002/elsc.70077","url":null,"abstract":"<p>The lipase-catalyzed oxidative functionalization of alkenes in Pickering emulsions (PE) offers a green and efficient alternative to conventional processes involving hazardous oxidants. Other investigated reaction media used for this alternative green pathway still suffer from enzyme deactivation and /or low specific reaction rate which limits their industrial adaptability. This study investigates the enzymatic synthesis of peroxyacetic acid, the first step in lipase-catalyzed oxidative functionalization, in a continuous membrane reactor. The effects of aqueous phase composition, modified silica nanoparticles, and operational parameters on reaction performance were systematically studied. Optimal conditions were obtained at pH 7, 100 mM buffer, and 5 g L_dp⁻¹ enzyme concentration. Surface-modified silica nanoparticles improved PE stability and interfacial catalytic efficiency, while maintaining comparable catalytic productivity. Hydrogen peroxide outperformed urea hydrogen peroxide, yielding a maximum product yield of 83% at a concentration of 45 mM in the influent solution and a space-time yield of 44.9 g_PAL⁻¹d⁻¹ at 386 mM. At 386 mM, the specific reaction rate (17.5 mmol g⁻¹ h⁻¹) was over twice that of reported single organic-phase systems. Despite the high peroxide concentrations, the enzyme displayed remarkable stability in PE due to the protective role of nanoparticles. This work provides critical insights into optimizing enzymatic oxidative functionalization in PE and their potential for sustainable industrial applications.</p><p><i>Practical application:</i> This study provides a foundation for the development of sustainable and efficient continuous processes for oxidative biotransformations using Pickering emulsions (PE). By enabling the enzymatic production of peracids, the first step in lipase-catalyzed oxidative functionalization, the process addresses key limitations of previously explored green reaction systems, such as enzyme deactivation and low specific activity. The PE system enhances emulsion stability and preserves enzyme activity under high oxidant concentrations, achieving high yields and space-time productivity in a membrane-based continuous reactor. Surface-modified silica nanoparticles further improve interfacial catalytic efficiency as well as emulsion stability. This approach is well-suited for selective and eco-friendly oxidation in the synthesis of fine chemicals, active pharmaceutical ingredients, and specialty materials. Additionally, the robustness of the system allows stable operation under harsh conditions, supporting the efficient integration of the second, chemical epoxidation step. These findings contribute to the broader implementation of continuous green chemistry technologies in industrial biocatalysis.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"26 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13054676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147638234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of 3D-Printed Resin Materials for Use in Microbial Bioprocess Applications: Suitability and Autoclavability","authors":"Carina Zierberg,&nbsp;Marcus Leppkes,&nbsp;Uta Bergstedt,&nbsp;Mathias Ulbricht,&nbsp;Heyko Jürgen Schultz","doi":"10.1002/elsc.70076","DOIUrl":"10.1002/elsc.70076","url":null,"abstract":"<p>Three-dimensional (3D) printing technologies are continually advancing, significantly broadening their application scope. By employing fused deposition modeling (FDM) to create iterative prototypes and stereolithography (SLA) for crafting functional components, these additive manufacturing techniques offer a highly efficient solution regarding cost and production speed. Although biocompatible materials are widely accessible, the biocompatibility of resins remains a contentious issue and requires specific evaluation for the relevant microorganisms. This study investigates the use of commercially available resins for biotechnology applications in a lab environment. Special emphasis was placed on material reuse, ensuring compatibility with autoclaving, the most common sterilization technique in laboratories. An analytical assessment was performed to identify potential leaching of polymers or resin components into the surrounding medium during autoclaving and to examine whether the materials' mechanical properties are preserved post-sterilization. The outcomes of this study may promote the broader use of 3D printing in biotechnology research by highlighting its sustainability, resource-saving potential, versatility, and relevance for various laboratory applications.</p><p><i>Practical application</i>: 3D printing has emerged as a crucial asset in research, highlighted by its versatility and adaptability. The application of synthetic resins in biotechnological contexts using stereolithography-based 3D printing technologies has been subject to considerable criticism in the past, primarily due to verified toxic effects. Although biocompatible resins are now commercially available, their functional performance and long-term safety have not been sufficiently studied. This study aims to facilitate the integration of 3D printing materials into standard biotechnological laboratory workflows by examining the viability of autoclaving as a sterilization technique. Additionally, for the first time, the reusability of the materials was assessed by testing their mechanical properties after multiple uses and repeated autoclaving. This approach seeks to simplify the use of 3D printing in biotechnological research, thereby facilitating its integration into routine laboratory workflows and supporting further advancements in the field.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"26 4","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147668879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Characterization of a 3D-Printable Membrane Aeration Module for Small-Scale Bioprocess Prototyping","authors":"Laurenz Köhne,&nbsp;Pia Elisabeth Lorenz,&nbsp;Marie-Luise Schlieker,&nbsp;Chantal Treinen,&nbsp;Marius Henkel","doi":"10.1002/elsc.70073","DOIUrl":"10.1002/elsc.70073","url":null,"abstract":"<p>Oxygen transfer is a critical design parameter in laboratory-scale bioprocess systems used for prototyping, process development, and scale-down studies of mammalian cell cultures, particularly when cultivating shear-sensitive mammalian cells. In this work, we present the design and characterization of a 3D-printed modular, membrane-based aeration module that enables bubble-free oxygen transfer in laboratory reference cell-cultivation systems. The aeration module was developed as an external, small-scale unit intended for flexible integration into laboratory bioreactors and perfusion setups. Fabricated via fused deposition modeling, the final design features a three-chamber membrane-stacking architecture that ensures mechanical stability, tightness, and biocompatibility, while allowing for straightforward adaptation through editable CAD files. The system was experimentally evaluated with respect to oxygen transfer performance under varying relative liquid flow rates and membrane configurations (PTFE and PVDF), each with two different pore sizes (0.22 µm and 0.45 µm). Key performance parameters of the aeration module were determined and include dissolved oxygen (DO) profiles, volumetric oxygen transfer coefficients (7.26 h⁻¹), oxygen transfer rates (OTRs) (max. 61.4 mg L⁻¹h⁻¹), and the pressure-normalized oxygen mass transfer rate (0.87 g m⁻²bar⁻¹h⁻¹). Overall, the modular design and quantified performance provide a versatile tool for rapid iteration and evaluation of membrane-based oxygenation strategies in early-stage bioprocess development.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"26 3","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147569231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Pharming: Advances, Applications, and Future Prospects in Biotechnology and Medicine","authors":"Md. Hridoy Ahmed,&nbsp;Md. Mustak Khan,&nbsp;Shishir Dutta,&nbsp;Md. Foyzur Rahman,&nbsp;Mohammad Shariful Islam,&nbsp;Md. Najmul Hosen,&nbsp;Md. Saad Hossain,&nbsp;Md. Aftabur Rahman,&nbsp;Md. Sadman Hasan Sahil,&nbsp;Tanjuma Tasnim Hira,&nbsp; Ifthesum,&nbsp;Ashikur Rahaman,&nbsp;Md. Afser Rabbi,&nbsp;Laila Khaleda","doi":"10.1002/elsc.70075","DOIUrl":"https://doi.org/10.1002/elsc.70075","url":null,"abstract":"<p>Genetically engineered plants incorporate the use of a novel bioreactor known as molecular pharming, which has a transformative view on the pharmaceutical industry. The technique enables mass production, at a low cost, and reproducibly of a large number of different protein-based drugs, vaccines, and industrial enzymes. This review-based study outlines the chronological evolution of molecular pharming, investigates its essential principles and elective applications, and meticulously compares it with other methods, namely conventional biomanufacturing. We present the numerous host organisms employed, the leading-edge genetic engineering procedure, and the sophisticated approaches for protein purification and extraction. Additionally, we deliver in-depth analysis of the noteworthy advantages that take place in molecular pharming as a captivating substitute, in conjunction with obstinate challenges, including concerns of public insights, intricate regulatory frameworks, and consideration for economic sustainability. Finally, this comprehensive study explores the promising direction, evolving innovations, and essential areas that influence future research to fully reveal the extensive potential of plant-based biopharmaceutical production for industrial strains and global health.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"26 3","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70075","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147565544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Pharming: Advances, Applications, and Future Prospects in Biotechnology and Medicine","authors":"Md. Hridoy Ahmed,&nbsp;Md. Mustak Khan,&nbsp;Shishir Dutta,&nbsp;Md. Foyzur Rahman,&nbsp;Mohammad Shariful Islam,&nbsp;Md. Najmul Hosen,&nbsp;Md. Saad Hossain,&nbsp;Md. Aftabur Rahman,&nbsp;Md. Sadman Hasan Sahil,&nbsp;Tanjuma Tasnim Hira,&nbsp; Ifthesum,&nbsp;Ashikur Rahaman,&nbsp;Md. Afser Rabbi,&nbsp;Laila Khaleda","doi":"10.1002/elsc.70075","DOIUrl":"https://doi.org/10.1002/elsc.70075","url":null,"abstract":"<p>Genetically engineered plants incorporate the use of a novel bioreactor known as molecular pharming, which has a transformative view on the pharmaceutical industry. The technique enables mass production, at a low cost, and reproducibly of a large number of different protein-based drugs, vaccines, and industrial enzymes. This review-based study outlines the chronological evolution of molecular pharming, investigates its essential principles and elective applications, and meticulously compares it with other methods, namely conventional biomanufacturing. We present the numerous host organisms employed, the leading-edge genetic engineering procedure, and the sophisticated approaches for protein purification and extraction. Additionally, we deliver in-depth analysis of the noteworthy advantages that take place in molecular pharming as a captivating substitute, in conjunction with obstinate challenges, including concerns of public insights, intricate regulatory frameworks, and consideration for economic sustainability. Finally, this comprehensive study explores the promising direction, evolving innovations, and essential areas that influence future research to fully reveal the extensive potential of plant-based biopharmaceutical production for industrial strains and global health.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"26 3","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.70075","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147565545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized Bioconversion of Naringenin to Hesperetin in Escherichia coli With Halide Methyltransferase-Mediated S-Adenosylmethionine Regeneration","authors":"Maik Wildhagen,&nbsp;Nina Malke,&nbsp;Qimin Wang,&nbsp;Xiaoying Zhuang,&nbsp;Sascha Beutel","doi":"10.1002/elsc.70069","DOIUrl":"10.1002/elsc.70069","url":null,"abstract":"<p>Hesperetin is a bioactive flavonoid with potential applications in pharmaceuticals and nutraceuticals, yet its low natural abundance limits commercial use. In this study, a two-step whole-cell bioconversion process was developed for the microbial production of hesperetin from naringenin in <i>Escherichia coli</i>. The 4-hydroxyphenylacetate-3-hydroxylase enzyme complex (HpaBC) enabled cytochrome P450-independent conversion of naringenin to eriodictyol. Subsequent 4′-<i>O</i>-methylation was achieved using a plant-derived flavonoid 4'-<i>O</i>-methyltransferase (FOMT) coupled with a halide methyltransferase (HMT) for in situ <i>S</i>-adenosylmethionine (SAM) regeneration. Enzyme activity was first confirmed individually in vitro and in vivo, followed by integration into recombinant whole-cell systems, co-expressing all desired enzymes. Process optimization through delayed co-substrate addition, improving induction conditions, and machine learning-guided parameter selection increased hesperetin yields up to 70.6% with minimal byproduct formation. This work demonstrates the feasibility of combining process development and digital optimization strategies for the sustainable production of methylated flavonoids in microbial systems. The resulting <i>E. coli</i> platform provides a scalable blueprint for future biotechnological applications involving cofactor-dependent plant secondary metabolism.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"26 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12930279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147289219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Fungal Developmental Structures on Mechanical Properties of Mycelial Materials","authors":"Kelsey Gray,&nbsp;Harley Edwards,&nbsp;Alexander G. Doan,&nbsp;Walker Huso,&nbsp;JungHun Lee,&nbsp;Wanwei Pan,&nbsp;Nelanne Bolima,&nbsp;Isha Gautam,&nbsp;Tuo Wang,&nbsp;Ranjan Srivastava,&nbsp;Marc Zupan,&nbsp;Mark R. Marten,&nbsp;Steven D. Harris","doi":"10.1002/elsc.70066","DOIUrl":"10.1002/elsc.70066","url":null,"abstract":"<p>This study explores how suppressing asexual development in <i>Aspergillus nidulans</i> enhances the mechanical properties of mycelial materials. Using four aconidial mutants <i>(∆brlA</i>, <i>∆flbA</i>, <i>∆fluG</i>, and <i>fadA^G42R</i>) lacking asexual development and a control strain (A28) that undergoes typical asexual development, we found that the absence of asexual development significantly improves mechanical strength. All mutants exhibited higher ultimate tensile strength (UTS) than the control, with <i>∆fluG</i> and <i>∆brlA</i> (fluffy nonsporulating, FNS phenotype) showing the highest UTS. Additionally, <i>fadA^G42R</i> and <i>∆flbA</i> (fluffy autolytic dominant, FAD phenotype) demonstrated significantly higher strain at failure (SF), linked to increased autolysis and lower dry cell mass compared to the control and FNS mutants. Solid-state NMR analysis suggests that autolysis in FAD mutants may disrupt galactofuranose-related processes, altering cell wall composition and contributing to higher elasticity. These findings suggest suppression of asexual development increases mycelial material strength, while autolysis mechanisms influence elasticity. This research highlights the potential for genetic manipulation in fungi to engineer advanced mycelial-based materials with tailored mechanical properties.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"26 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12930277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147289432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progressive Cavity Pumps—A Comparison of New Technology for Gradient Bioprinting to Existing Extrusion Methods","authors":"Franz Moser,&nbsp;Shiva Rahmani,&nbsp;Tomasz Jungst","doi":"10.1002/elsc.70070","DOIUrl":"10.1002/elsc.70070","url":null,"abstract":"<p>Extrusion-based Bioprinting is a key technology in biofabrication, yet the choice of extrusion method is often limited to established techniques built into most bioprinters, limiting the print fidelity and more demanding applications like printing material gradients. In this technical report we compare the emerging method of progressive cavity pump with established technologies such as pneumatic extrusion and syringe pump-based printing setups. The three methods were compared for their accuracy and precision in extruding 35% Pluronic F127, followed by test simulating different flow profiles, material dependency and ability to transfer between hardware setups. The progressive cavity pumps showed the most advantageous behavior for gradient printing, with the syringe pumps needing more iterations for stable extrusion and the pneumatic extrusion enabling high-volume extrusion but showing lower precision. This was further shown with the printing of different gradients.</p>","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"26 2","pages":""},"PeriodicalIF":3.0,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12928107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147282771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}