Engineering in Life Sciences最新文献_第9页

Regulation on C2-C8 carboxylic acid biosynthesis from anaerobic CO2 fermentation 厌氧CO2发酵对C2‐C8羧酸生物合成的调控

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2022-12-13 DOI: 10.1002/elsc.202200069

Wanling Wu, Zhiqi Li, Guangqing Liu, Ling Zhou, Wen Wang

{"title":"Regulation on C2-C8 carboxylic acid biosynthesis from anaerobic CO2 fermentation","authors":"Wanling Wu, Zhiqi Li, Guangqing Liu, Ling Zhou, Wen Wang","doi":"10.1002/elsc.202200069","DOIUrl":"10.1002/elsc.202200069","url":null,"abstract":"Bioconversion of CO2 into liquid fuels or chemicals, preferred medium chain carboxylic acids (caproic and caprylic acid), is an attractive CO2 utilization technology. The present study aims to investigate the effects of different ratios of H2/CO2 on regulating the distribution of C2-C8 carboxylic acid products, while the headspace pressure of 1.5 bar was set to amplify the effect of different ratios. The H2/CO2 ratio of 4:1 was more suitable for preparing acetic acid, where the highest acetic acid yield was 17.5 g/L. And the H2/CO2 ratio of 2:1 showed excellent chain elongation ability with the highest n-caprylic yield of 2.4 g/L. Additionally, the actual H2/CO2 ratios of 4:1 reactors were higher than that in 2:1 may be course chain elongation often accompanied by H2 production. The 16S rRNA genes analysis shows that the genus Terrisporobacter and Coriobacteriales may be related to acetic acid production enriched in H2/CO2 ratio 4:1 reactors, and the genus Clostridium and Paenibacillaceae may associate with the chain elongation pathway were enriched in H2/CO2 ratio 2:1 reactors.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202200069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43305302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Picture: Engineering in Life Sciences 12'22 封面图片：生命科学工程12'22

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2022-12-08 DOI: 10.1002/elsc.202270121

引用次数: 0

Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production 富集同源丙酮，将H2/CO2转化为酸和乙醇，同时产生甲烷

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2022-12-07 DOI: 10.1002/elsc.202200027

Yaxue He, Chiara Cassarini, Piet N.L. Lens

{"title":"Enrichment of homoacetogens converting H2/CO2 into acids and ethanol and simultaneous methane production","authors":"Yaxue He, Chiara Cassarini, Piet N.L. Lens","doi":"10.1002/elsc.202200027","DOIUrl":"https://doi.org/10.1002/elsc.202200027","url":null,"abstract":"An anaerobic granular sludge was enriched to utilize H2/CO2 in a continuous gas-fed up-flow anaerobic sludge reactor by applying operating conditions expected to produce acetic acid, butyric acid, and ethanol. Three stages of fermentation were found: Stage I with acetic acid accumulation with the highest concentration of 35 mM along with a pH decrease from initial 6 to 4.5. In Stage II, H2/CO2 was replaced by 100% H2 to induce solventogenesis, whereas butyric acid was produced with the highest concentration of 2.5 mM. At stage III with 10 µM tungsten (W) addition, iso-valeric acid, valeric acid, and caproic acid were produced at pH 4.5–5.0. In the batch tests inoculated with the enriched sludge taken from the bioreactor (day 70), however, methane production occurred at pH 6. Exogenous 15 mM acetate addition enhanced both the H2 and CO2 consumption rate compared to exogenous 10, 30, and 45 mM acetate by the enriched sludge. Exogenous acetate was failed to be converted to ethanol using H2 as electron donor by the enriched acetogens.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"23 2","pages":""},"PeriodicalIF":2.7,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202200027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50123838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Issue Information 问题信息

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2022-12-01 DOI: 10.1111/pace.14521