Engineering in Life Sciences最新文献_第9页

Flavonoids, gut microbiota, and host lipid metabolism 类黄酮、肠道微生物群和宿主脂质代谢

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-11-13 DOI: 10.1002/elsc.202300065

Miao Zhou, Jie Ma, Meng Kang, Wenjie Tang, Siting Xia, Jie Yin, Yulong Yin

引用次数: 0

Cover Picture: Engineering in Life Sciences 11'23 封面图片:Engineering in Life Sciences 11'23

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-11-08 DOI: 10.1002/elsc.202370111

引用次数: 0

Endospore production of Bacillus spp. for industrial use 工业用芽孢杆菌的孢子内生产

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-10-12 DOI: 10.1002/elsc.202300013

Riekje Biermann, Sascha Beutel

引用次数: 0

Gut microbiota and childhood malnutrition: Understanding the link and exploring therapeutic interventions 肠道微生物群与儿童营养不良：了解联系并探索治疗干预措施

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-10-05 DOI: 10.1002/elsc.202300070

Sevda Zoghi, Fatemah Sadeghpour Heravi, Zeinab Nikniaz, Masoud Shirmohamadi, Seyed Yaghoub Moaddab, Hamed Ebrahimzadeh Leylabadlo

{"title":"Gut microbiota and childhood malnutrition: Understanding the link and exploring therapeutic interventions","authors":"Sevda Zoghi, Fatemah Sadeghpour Heravi, Zeinab Nikniaz, Masoud Shirmohamadi, Seyed Yaghoub Moaddab, Hamed Ebrahimzadeh Leylabadlo","doi":"10.1002/elsc.202300070","DOIUrl":"10.1002/elsc.202300070","url":null,"abstract":"Childhood malnutrition is a metabolic condition that affects the physical and mental well-being of children and leads to resultant disorders in maturity. The development of childhood malnutrition is influenced by a number of physiological and environmental factors including metabolic stress, infections, diet, genetic variables, and gut microbiota. The imbalanced gut microbiota is one of the main environmental risk factors that significantly influence host physiology and childhood malnutrition progression. In this review, we have evaluated the gut microbiota association with undernutrition and overnutrition in children, and then the quantitative and qualitative significance of gut dysbiosis in order to reveal the impact of gut microbiota modification using probiotics, prebiotics, synbiotics, postbiotics, fecal microbiota transplantation, and engineering biology methods as new therapeutic challenges in the management of disturbed energy homeostasis. Understanding the host–microbiota interaction and the remote regulation of other organs and pathways by gut microbiota can improve the effectiveness of new therapeutic approaches and mitigate the negative consequences of childhood malnutrition.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"24 5","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135482137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Picture: Engineering in Life Sciences 10'23 封面图片：生命科学工程10’23

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-10-03 DOI: 10.1002/elsc.202370101

引用次数: 0

Bioprocess development for endospore production by Bacillus coagulans using an optimized chemically defined medium 使用优化的化学限定培养基由凝结芽孢杆菌生产内生孢子的生物工艺开发

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-09-15 DOI: 10.1002/elsc.202300210

Riekje Biermann, Laura Rösner, Lisa-Marie Beyer, Laura Niemeyer, Sascha Beutel

{"title":"Bioprocess development for endospore production by Bacillus coagulans using an optimized chemically defined medium","authors":"Riekje Biermann, Laura Rösner, Lisa-Marie Beyer, Laura Niemeyer, Sascha Beutel","doi":"10.1002/elsc.202300210","DOIUrl":"https://doi.org/10.1002/elsc.202300210","url":null,"abstract":"Bacillus coagulans is a promising probiotic, because it combines probiotic properties of Lactobacillus and the ability of Bacillus to form endospores. Due to this hybrid relationship, cultivation of this organism is challenging. As the probiotics market continues to grow, there is a new focus on the production of these microorganisms. In this work, a strain‐specific bioprocess for B. coagulans was developed to support growth on one hand and ensure sporulation on the other hand. This circumstance is not trivial, since these two metabolic states are contrary. The developed bioprocess uses a modified chemically defined medium which was further investigated in a one‐factor‐at‐a‐time assay after adaptation. A transfer from the shake flask to the bioreactor was successfully demonstrated in the scope of this work. The investigated process parameters included temperature, agitation and pH‐control. Especially the pH‐control improved the sporulation in the bioreactor when compared to shake flasks. The bioprocess resulted in a sporulation efficiency of 80%–90%. This corresponds to a sevenfold increase in sporulation efficiency due to a transfer to the bioreactor with pH‐control. Additionally, a design of experiment (DoE) was conducted to test the robustness of the bioprocess. This experiment validated the beforementioned sporulation efficiency for the developed bioprocess. Afterwards the bioprocess was then scaled up from a 1 L scale to a 10 L bioreactor scale. A comparable sporulation efficiency of 80% as in the small scale was achieved. The developed bioprocess facilitates the upscaling and application to an industrial scale, and can thus help meet the increasing market for probiotics.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"23 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300210","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50134360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of a perfusion reactor and continuous precipitation in an entirely membrane-based process for antibody capture 在完全基于膜的抗体捕获过程中整合灌注反应器和连续沉淀。

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-09-07 DOI: 10.1002/elsc.202300219

Gabriele Recanati, Magdalena Pappenreiter, Christoph Gstoettner, Patrick Scheidl, Elena Domínguez Vega, Bernhard Sissolak, Alois Jungbauer

{"title":"Integration of a perfusion reactor and continuous precipitation in an entirely membrane-based process for antibody capture","authors":"Gabriele Recanati, Magdalena Pappenreiter, Christoph Gstoettner, Patrick Scheidl, Elena Domínguez Vega, Bernhard Sissolak, Alois Jungbauer","doi":"10.1002/elsc.202300219","DOIUrl":"10.1002/elsc.202300219","url":null,"abstract":"Continuous precipitation coupled with continuous tangential flow filtration is a cost-effective alternative for the capture of recombinant antibodies from crude cell culture supernatant. The removal of surge tanks between unit operations, by the adoption of tubular reactors, maintains a continuous harvest and mass flow of product with the advantage of a narrow residence time distribution (RTD). We developed a continuous process implementing two orthogonal precipitation methods, CaCl2 precipitation for removal of host-cell DNA and polyethylene glycol (PEG) for capturing the recombinant antibody, with no influence on the glycosylation profile. Our lab-scale prototype consisting of two tubular reactors and two stages of tangential flow microfiltration was continuously operated for up to 8 days in a truly continuous fashion and without any product flow interruption, both as a stand-alone capture and as an integrated perfusion-capture. Furthermore, we explored the use of a negatively charged membrane adsorber for flow-through anion exchange as first polishing step. We obtained a product recovery of approximately 80% and constant product quality, with more than two logarithmic reduction values (LRVs) for both host-cell proteins and host-cell DNA by the combination of the precipitation-based capture and the first polishing step.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"23 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202300219","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41114057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Picture: Engineering in Life Sciences 9'23 封面图片：生命科学工程9’23

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-09-01 DOI: 10.1002/elsc.202370091

引用次数: 0

Investigation and evaluation of a 3D-printed optical modified cultivation vessel for improved scattered light measurement of biotechnologically relevant organisms 用于改进生物技术相关生物散射光测量的3d打印光学修饰培养容器的研究和评估

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-08-23 DOI: 10.1002/elsc.202300204

Johanna S. Rehfeld, Louis M. Kuhnke, Christian Ude, Gernot T. John, Sascha Beutel

{"title":"Investigation and evaluation of a 3D-printed optical modified cultivation vessel for improved scattered light measurement of biotechnologically relevant organisms","authors":"Johanna S. Rehfeld, Louis M. Kuhnke, Christian Ude, Gernot T. John, Sascha Beutel","doi":"10.1002/elsc.202300204","DOIUrl":"10.1002/elsc.202300204","url":null,"abstract":"In the field of bioprocess development miniaturization, parallelization and flexibility play a key role reducing costs and time. To precisely meet these requirements, additive manufacturing (3D-printing) is an ideal technology. 3D-printing enables rapid prototyping and cost-effective fabrication of individually designed devices with complex geometries on demand. For successful bioprocess development, monitoring of process-relevant parameters, such as pH, dissolved oxygen (DO), and biomass, is crucial. Online monitoring is preferred as offline sampling is time-consuming and leads to loss of information. In this study, 3D-printed cultivation vessels with optical prisms are evaluated for the use in upstream processes of different industrially relevant microorganisms and cell lines. It was shown, that the 3D-printed optically modified well (OMW) is of benefit for a wide range of biotechnologically relevant microorganisms and even for mammalian suspension cells. Evaluation tests with Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae, and Chinese hamster ovary (CHO) cells were performed, providing highly reproducible results. Growth behavior of OMW cultures was comparable to behavior of shake flask (SF) cultivations and the signal to noise ratio in online biomass measurement was shown to be reduced up to 95.8% by using the OMW. Especially the cultivation phases with low turbidity respective optical densities below 1.0 rel.AU could be monitored accurately for the first time. Furthermore, it was demonstrated that the 3D-printed optics are transferable to different well geometries and sizes, enabling efficient biomass monitoring for individual requirements with tailor-made 3D-printed cultivation vessels in small scale.","PeriodicalId":11678,"journal":{"name":"Engineering in Life Sciences","volume":"23 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b5/2b/ELSC-23-e2300204.PMC10472911.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10152389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Meta-omics assisted microbial gene and strain resources mining in contaminant environment 元组学辅助污染环境中微生物基因和菌株资源的挖掘

IF 2.7 4区生物学

Engineering in Life Sciences Pub Date : 2023-08-18 DOI: 10.1002/elsc.202300207

Yiqun Huang, Haiyang Hu, Tingting Zhang, Weiwei Wang, Wenzhao Liu, Hongzhi Tang

引用次数: 0