Endothelium : journal of endothelial cell research最新文献_第8页

Chemokine transport across human vascular endothelial cells. 趋化因子在人血管内皮细胞中的转运。

Endothelium : journal of endothelial cell research Pub Date : 2007-01-01 DOI: 10.1080/10623320601177312

Elodie Mordelet, Heather A Davies, Philippa Hillyer, Ignacio A Romero, David Male

{"title":"Chemokine transport across human vascular endothelial cells.","authors":"Elodie Mordelet, Heather A Davies, Philippa Hillyer, Ignacio A Romero, David Male","doi":"10.1080/10623320601177312","DOIUrl":"https://doi.org/10.1080/10623320601177312","url":null,"abstract":"Leukocyte migration across vascular endothelium is mediated by chemokines that are either synthesized by the endothelium or transferred across the endothelium from the tissue. The mechanism of transfer of two chemokines, CXCL10 (interferon gamma-inducible protein [IP]-10) and CCL2 (macrophage chemotactic protein [MCP]-1), was compared across dermal and lung microvessel endothelium and saphenous vein endothelium. The rate of transfer depended on both the type of endothelium and the chemokine. The permeability coefficient (Pe) for CCL2 movement across saphenous vein was twice the value for dermal endothelium and four times that for lung endothelium. In contrast, the Pe value for CXCL10 was lower for saphenous vein endothelium than the other endothelia. The differences in transfer rate between endothelia was not related to variation in paracellular permeability using a paracellular tracer, inulin, and immunoelectron microscopy showed that CXCL10 was transferred from the basal membrane in a vesicular compartment, before distribution to the apical membrane. Although all three endothelia expressed high levels of the receptor for CXCL10 (CXCR3), the transfer was not readily saturable and did not appear to be receptor dependent. After 30 min, the chemokine started to be reinternalized from the apical membrane in clathrin-coated vesicles. The data suggest a model for chemokine transcytosis, with a separate pathway for clearance of the apical surface.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":"14 1","pages":"7-15"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320601177312","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26608649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Elevated glucose attenuates agonist- and flow-stimulated endothelial nitric oxide synthase activity in microvascular retinal endothelial cells. 葡萄糖升高可减弱微血管视网膜内皮细胞中激动剂和血流刺激的内皮型一氧化氮合酶活性。

Endothelium : journal of endothelial cell research Pub Date : 2007-01-01 DOI: 10.1080/10623320601177213

Paul Connell, Tony Walshe, Gail Ferguson, Wei Gao, Colm O'Brien, Paul A Cahill

{"title":"Elevated glucose attenuates agonist- and flow-stimulated endothelial nitric oxide synthase activity in microvascular retinal endothelial cells.","authors":"Paul Connell, Tony Walshe, Gail Ferguson, Wei Gao, Colm O'Brien, Paul A Cahill","doi":"10.1080/10623320601177213","DOIUrl":"https://doi.org/10.1080/10623320601177213","url":null,"abstract":"Impaired vasoactive release of opposing vasodilator and vasoconstrictor mediators due to endothelial dysfunction is integral to the pathogenesis of diabetic retinopathy. The aim of this study was to determine the effect of hyperglycemia on the expression of endothelial nitric oxide synthase (eNOS) and the release of nitric oxide (NO) in bovine microvascular retinal endothelial cells (BRECs) under both static (basal and acetylcholine stimulated) and flow (laminar shear stress [10 dynes/cm2 and pulsatile flow 0.3 to 23 dynes/cm2) conditions using a laminar shear apparatus and an in vitro perfused transcapillary culture system. The activity and expression of eNOS, measured by nitrate levels and immunoblot, respectively, were determined following exposure of BRECs to varying concentrations of glucose and mannitol (0 to 25 mM). Under static conditions the expression of eNOS decreased significantly following exposure to increasing concentrations of glucose when compared to osmotic mannitol controls and was accompanied by a significant dose-dependent decrease in nitrate levels in conditioned medium. The acetylcholine stimulated increase in NO release (2.0 +/- 0.3-fold) was significantly reduced by 55% +/- 5% and 65% +/- 4.5% following exposure to 16 and 25 mM glucose, respectively, when compared to osmotic controls. In parallel studies, glucose significantly inhibited both laminar shear stress and pulsatile flow-induced activity when compared to mannitol. We conclude that hyperglycemia impairs agonist- and flow-dependent release of NO in retinal microvascular endothelial cells and may thus contribute to the vascular endothelial dysfunction and impaired autoregulation of diabetic retinopathy.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":"14 1","pages":"17-24"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320601177213","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26608650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

A novel lysophospholipid- and pH-sensitive receptor, GPR4, in brain endothelial cells regulates monocyte transmigration. 脑内皮细胞中的一种新的溶血磷脂和ph敏感受体GPR4调节单核细胞的转运。

Endothelium : journal of endothelial cell research Pub Date : 2007-01-01 DOI: 10.1080/10623320601177288

Fei Huang, Dolly Mehta, Sanda Predescu, Kwang S Kim, Hazel Lum

{"title":"A novel lysophospholipid- and pH-sensitive receptor, GPR4, in brain endothelial cells regulates monocyte transmigration.","authors":"Fei Huang, Dolly Mehta, Sanda Predescu, Kwang S Kim, Hazel Lum","doi":"10.1080/10623320601177288","DOIUrl":"https://doi.org/10.1080/10623320601177288","url":null,"abstract":"Abundant evidence documents the highly proinflammatory actions of lysophosphatidylcholine (LPC). Further, LPC, found in high amounts in oxidized low-density lipoprotein (LDL), is implicated as an atherogenic factor. In endothelial cells, LPC impairs endothelial barrier function through GPR4, a novel receptor hypothesized to be sensitive to LPC and protons. The authors investigated the stimulation by LPC or low pH of GPR4 in human brain microvascular endothelial cells (HBMECs) and whether the activated GPR4 regulates in vitro monocyte transmigration. The results indicated that HBMECs stimulated by LPC (5 microM), but not low pH, showed a twofold increase in monocyte transmigration. Using retroviruses containing siRNA to GPR4, a > 60% reduction of GPR4 expression resulted in blockade of the LPC-stimulated transmigration. The inhibited response was restored by co-expression with an small interference RNA (siRNA)-resistant, but functional, GPR4 mutant construct. To investigate potential signaling mechanisms, the siRNA-mediated knockdown of GPR4 also prevented LPC-induced RhoA activation. C3 transferase, a Rho inhibitor, prevented approximately approximately 65% of the LPC-stimulated transmigration. LPC also increased MLC phosphorylation by 5 min, which was inhibited by the Rho kinase inhibitor, Y-27632 (10 microM) or ML-7 (myosin light chain kinase (MLCK) inhibitor). The findings indicate that the proinflammatory and atherogenic LPC stimulated endothelial GPR4, which promoted monocyte transmigration through a RhoA-dependent pathway.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":"14 1","pages":"25-34"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320601177288","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26608652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Immortalization and characterization of porcine ventricular endocardial endothelial cells. 猪心室心内膜内皮细胞的永生化及特性研究。

Endothelium : journal of endothelial cell research Pub Date : 2007-01-01 DOI: 10.1080/10623320601177353

Leena Kuruvilla, Santhoshkumar T R, Chandrasekharan Cheranellore Kartha

引用次数: 15

Clinical assessment of endothelial function. 内皮功能的临床评估。

Endothelium : journal of endothelial cell research Pub Date : 2006-11-01 DOI: 10.1080/10623320601061573

Mark R Adams

{"title":"Clinical assessment of endothelial function.","authors":"Mark R Adams","doi":"10.1080/10623320601061573","DOIUrl":"https://doi.org/10.1080/10623320601061573","url":null,"abstract":"The endothelium is crucial in the maintenance of normal vascular function and disturbance of this balance is a key early event in the development of vascular disease. A wide range of techniques currently exists for assessment of endothelial function in both the coronary and the peripheral vasculature. Many of these in vivo tests have concentrated on measuring nitric oxide bioavailability, however more recently methods for measuring other vascular parameters, such as tissue-plasminogen activator release, have been used. Furthermore indirect systemic measurements of endothelial function and endothelial progenitor cells have been investigated. These methods have given great insights into the pathophysiology of atherosclerosis and have aided in the development of a number of antiatherosclerotic therapies. Importantly the methods used to date for assessing endothelial function in vivo are accurate, reproducible and correlate with the future risk of cardiovascular events. The development of new techniques and the constant refinement of established techniques suggest that many more insights are to be gained from clinical assessment of endothelial function.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":"13 6","pages":"367-74"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320601061573","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26500667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Flow-mediated signaling modulates endothelial cell phenotype. 血流介导的信号传导调节内皮细胞表型。

Endothelium : journal of endothelial cell research Pub Date : 2006-11-01 DOI: 10.1080/10623320601061599

Gwenaele Garin, Bradford C Berk

引用次数: 49

The endothelium and inflammation. 内皮和炎症。

Endothelium : journal of endothelial cell research Pub Date : 2006-11-01 DOI: 10.1080/10623320601061862

Thomas Trepels, Andreas M Zeiher, Stephan Fichtlscherer

{"title":"The endothelium and inflammation.","authors":"Thomas Trepels, Andreas M Zeiher, Stephan Fichtlscherer","doi":"10.1080/10623320601061862","DOIUrl":"https://doi.org/10.1080/10623320601061862","url":null,"abstract":"The vascular endothelium contributes to and is affected by inflammatory processes. Disturbance of the endothelium's morphologic and functional integrity in response to mechanical, immunologic, and chemical injuries reflects the first step in the pathophysiological cascade of atherosclerotic disorders. At the site of an endothelial injury, invading inflammatory cells producing numerous proinflammatory factors promote and amplify both local and systemic inflammation. These early changes on a cellular and subcellular level that precede the clinical manifestation of atherosclerosis are associated with loss of profound physiological functions of the endothelium. One pivotal function of the endothelium is nitric oxide-mediated regulation of vessel tone and blood flow according to the local requirements. The assessment of nitric oxide-mediated endothelial function by different methods revealed a close relation between inflammatory activation and endothelial dysfunction in healthy volunteers, patients at risk, and patients with established cardiovascular disease. Moreover, anti-inflammatory therapeutic interventions do not only have a positive impact on disease progression, but also on endothelial function, thus further providing an indirect line of evidence linking inflammation with endothelial dysfunction.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":"13 6","pages":"423-9"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320601061862","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26501120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 67

Vascular grafts and the endothelium. 血管移植物和内皮。

Endothelium : journal of endothelial cell research Pub Date : 2006-11-01 DOI: 10.1080/10623320601061615

Michel R Hoenig, Gordon R Campbell, Julie H Campbell

引用次数: 37

Endothelial progenitor cells. 内皮祖细胞。

Endothelium : journal of endothelial cell research Pub Date : 2006-11-01 DOI: 10.1080/10623320601061656

Brendan Doyle, Pat Metharom, Noel M Caplice

{"title":"Endothelial progenitor cells.","authors":"Brendan Doyle, Pat Metharom, Noel M Caplice","doi":"10.1080/10623320601061656","DOIUrl":"https://doi.org/10.1080/10623320601061656","url":null,"abstract":"The identification of circulating endothelial progenitor cells (EPCs) has prompted an explosion of interest in postnatal vasculogenesis and the role of this mechanism in human health and disease. Previously considered restricted to the embryonic phase, the differentiation in situ of progenitor cells to vascular endothelium is now known to occur in the adult. A role for EPCs in the modulation of angiogenesis has also been recognized. These cells are enriched in the mononuclear cell fraction of peripheral blood but have also been isolated from bone marrow, the vessel wall, and a number of other organs and tissues. Accumulating data suggest an important vasculoprotective function for EPCs, although a maladaptive role underpinning a variety of angiogenesis-dependent diseases is also being investigated. Encouraging results observed with experimental and early human trials of EPC-based regenerative therapies have further underscored the significance of this recently discovered cell type. Notwithstanding the scope and pace of these developments, a number of challenges remain: the precise ontogeny and lineage of these cells is unknown, the true extent to which EPCs participate in neovascularization and vascular repair is still uncertain, and the efficacy of EPC-based regenerative therapies has yet to be proven in randomized controlled trials.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":"13 6","pages":"403-10"},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320601061656","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26500670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Promoting vascular regeneration as an alternative to conventional angioplasty-based intervention. 促进血管再生作为传统血管成形术干预的替代方案。

Endothelium : journal of endothelial cell research Pub Date : 2006-11-01 DOI: 10.1080/10623320601066812

Annemarie M Noordeloos, Thomas Soullié, Henricus J Duckers, P W J C Serruys

引用次数: 7