Endothelium : journal of endothelial cell research最新文献_第9页

Novel therapies targeting vascular endothelium. 针对血管内皮的新型疗法。

Endothelium : journal of endothelial cell research Pub Date : 2006-11-01 DOI: 10.1080/10623320601061714

Dimitris Tousoulis, Charalambos Antoniades, Nikolaos Koumallos, Kyriakoula Marinou, Elli Stefanadi, George Latsios, Christodoulos Stefanadis

{"title":"Novel therapies targeting vascular endothelium.","authors":"Dimitris Tousoulis, Charalambos Antoniades, Nikolaos Koumallos, Kyriakoula Marinou, Elli Stefanadi, George Latsios, Christodoulos Stefanadis","doi":"10.1080/10623320601061714","DOIUrl":"10.1080/10623320601061714","url":null,"abstract":"Endothelial dysfunction has been identified as a major mechanism involved in all the stages of atherogenesis. Evaluation of endothelial function seems to have a predictive role in humans, and therapeutic interventions improving nitric oxide bioavailability in the vasculature may improve the long-term outcome in healthy individuals, high-risk subjects, or patients with advanced atherosclerosis. Several therapeutic strategies are now available, targeting both the synthesis and oxidative inactivation of nitric oxide (NO) in human vasculature. Statins seem to be currently the most powerful category of these agents, improving endothelial function and decreasing cardiovascular risk after long-term administration. Other cardiovascular agents improving endothelial function in humans are angiotensin-converting enzyme inhibitors/angiotensin receptors blockers, which increase NO bioavailability by modifying the rennin-angiotensin-aldosterone system. Newer therapeutic approaches targeting endothelial dysfunction in specific disease states include insulin sensitizers, L-arginine (the substrate for endothelial NO synthase [eNOS]) as well as substances that target eNOS \"coupling,\" such as folates or tetrahydrobiopterin. Although there are a variety of strategies to improve NO bioavailability in human endothelium, it is still unclear whether they have any direct benefit at a clinical level.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320601061714","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26500671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Asymmetric subcellular distribution of glucose transporters in the endothelium of small contractile arteries. 小动脉收缩内皮中葡萄糖转运体的不对称亚细胞分布。

Endothelium : journal of endothelial cell research Pub Date : 2006-09-01 DOI: 10.1080/10623320600972085

N Gaudreault, D R L Scriven, E D W Moore

{"title":"Asymmetric subcellular distribution of glucose transporters in the endothelium of small contractile arteries.","authors":"N Gaudreault, D R L Scriven, E D W Moore","doi":"10.1080/10623320600972085","DOIUrl":"https://doi.org/10.1080/10623320600972085","url":null,"abstract":"The authors have recently reported the presence and asymmetric distribution of the glucose transporters GLUT-1 to -5 and SGLT-1 in the endothelium of rat coronary artery (Gaudreault et al. 2004, Diabetologica, 47, 2081-2092). In the present study the authors investigate and compare the presence and subcellular distribution of the classic glucose transporter isoforms in endothelial cells of cerebral, renal, and mesenteric arteries. The GLUTs and SGLT-1 were examined with immunohistochemistry and wide-field fluorescence microscopy coupled to deconvolution in en face preparation of intact artery. We identified GLUT-1 to -5 and SGLT-1 in the endothelial cells of all three vascular beds. The relative level of expression for each isoform was found comparable amongst arteries. Clusters of the glucose transporter isoforms were found at a high density in proximity to the cell-to-cell junctions. In addition, a consistent asymmetric distribution of GLUT-1 to -5 was found, predominantly located on the abluminal side of the endothelium in all three vascular beds examined (ranging from 68% to 91%, p<.05). The authors conclude that the expression and subcellular distribution of glucose transporters are similar in endothelial cells from vascular beds of comparable diameter and suggest that their subcellular organization may facilitate transendothelial transport of glucose in small contractile arteries.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320600972085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26352040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Evidence of vascular damage in dengue disease: demonstration of high levels of soluble cell adhesion molecules and circulating endothelial cells. 登革热中血管损伤的证据:高水平的可溶性细胞粘附分子和循环内皮细胞的证明。

Endothelium : journal of endothelial cell research Pub Date : 2006-09-01 DOI: 10.1080/10623320600972135

José E Cardier, Betty Rivas, Egidio Romano, Alan L Rothman, Carlos Perez-Perez, Mariangel Ochoa, Ana M Caceres, Marisabel Cardier, Napoleón Guevara, Roberto Giovannetti

{"title":"Evidence of vascular damage in dengue disease: demonstration of high levels of soluble cell adhesion molecules and circulating endothelial cells.","authors":"José E Cardier, Betty Rivas, Egidio Romano, Alan L Rothman, Carlos Perez-Perez, Mariangel Ochoa, Ana M Caceres, Marisabel Cardier, Napoleón Guevara, Roberto Giovannetti","doi":"10.1080/10623320600972135","DOIUrl":"https://doi.org/10.1080/10623320600972135","url":null,"abstract":"Clinical evidence suggests that vascular damage plays a key role in the pathophysiology of dengue hemorrhagic fever (DHF). In this study, the authors tested this hypothesis by examining the levels of soluble intercellular adhesion molecule and vascular cell adhesion molecule (sICAM-1 and sVCAM-1), and the presence of circulating endothelial cells (CECs), as evidence of vascular damage, in peripheral blood from DHF patients (n=13). A significant increase in plasma levels of sICAM-1 (n=12) and sVCAM-1 (n=13) was detected by enzyme-linked immunosorbent assay (ELISA) in DHF patients, compared with healthy individuals. Increased numbers of CECs, as detected by the expression of endothelial cell markers (ICAM-1, platelet cell adhesion molecule [PCAM]-1, and CD36) with flow cytometry, were observed in DHF patients (n=4), compared to healthy subjects. The high levels of sICAM-1 and sVCAM-1, together with the presence of CECs in DHF patients, provide further evidence of endothelium damage and activation in DHF patients.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320600972135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26352041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 69

A study of physiologic angiogenesis in the human using the dental pulp as an in vivo model. 以牙髓为体内模型的生理性血管生成研究。

Endothelium : journal of endothelial cell research Pub Date : 2006-09-01 DOI: 10.1080/10623320600972101

Frank Arfuso

引用次数: 6

Alkaline pH-induced extracellular regulated protein kinase activation in brain microvascular endothelial cells: differential effects of Tris and lowered CO2. 碱性ph诱导的脑微血管内皮细胞胞外调节蛋白激酶活化:Tris和降低CO2的差异效应。

Endothelium : journal of endothelial cell research Pub Date : 2006-09-01 DOI: 10.1080/10623320600972119

Gregory T Motz, Mario Zuccarello, Robert M Rapoport

引用次数: 2

Soluble endothelial adhesion molecule concentration in patients with aortic coarctation. 主动脉缩窄患者可溶性内皮粘附分子浓度的变化。

Endothelium : journal of endothelial cell research Pub Date : 2006-09-01 DOI: 10.1080/10623320600972143

Pavel Osmancik, József Bocsi, Jörg Hambsch, Peter Schneider, Attila Tárnok

{"title":"Soluble endothelial adhesion molecule concentration in patients with aortic coarctation.","authors":"Pavel Osmancik, József Bocsi, Jörg Hambsch, Peter Schneider, Attila Tárnok","doi":"10.1080/10623320600972143","DOIUrl":"https://doi.org/10.1080/10623320600972143","url":null,"abstract":"Coarctation of aorta (CoA) is often associated with development of vascular abnormalities and hypertension despite successful correction. The aim of the study was to compare concentrations of adhesion molecules and interleukin-6 (IL-6), an inflammatory cytokine in following groups: children with CoA before operation, chidren with CoA after operation, and healthy control children. Seventeen children with CoA and 18 healthy children (control) were investigated. Blood samples were taken 1 day preoperatively and during followup (10.2+/-7.5 months). Serum concentrations of soluble E- and L-selectin, intercellular adhesion molecule-1 (sICAM-1), and IL-6 were measured by enzyme-linked immunosorbent assay (ELISA). On arms, systolic and diastolic blood pressures decreased after surgery. On legs, only systolic, but not diastolic, blood pressure increased significantly. There was no difference in the concentrations of IL-6, sE-, sL-selectin, or sICAM-1 before and after CoA repair. Postoperative ICAM-1 concentration in children with CoA was significantly higher compared to control (321.7+/-93.4 versus 248.8+/-84.3 ng/mL, P=.002). Only preoperative concentration of L-selectin was higher in children with CoA compared to control (1617.7+/-387.5 ng/mL versus 1271.1+/-266.6 ng/mL). The correction of CoA leads to normalization of leukocyte activity. The markers of endothelial damage and proinflammatory activity are not significantly changed by correction of CoA in young children.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320600972143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26351456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Elevation of intracellular cAMP up-regulated thrombomodulin mRNA in cultured vascular endothelial cells derived from spontaneous type-II diabetes mellitus model rat. 自发性ii型糖尿病模型大鼠血管内皮细胞内cAMP上调血栓调节蛋白mRNA的表达。

Endothelium : journal of endothelial cell research Pub Date : 2006-09-01 DOI: 10.1080/10623320600972051

Masanori Sunagawa, Seiji Shimada, Kazuhiko Hanashiro, Mariko Nakamura, Tadayoshi Kosugi

{"title":"Elevation of intracellular cAMP up-regulated thrombomodulin mRNA in cultured vascular endothelial cells derived from spontaneous type-II diabetes mellitus model rat.","authors":"Masanori Sunagawa, Seiji Shimada, Kazuhiko Hanashiro, Mariko Nakamura, Tadayoshi Kosugi","doi":"10.1080/10623320600972051","DOIUrl":"https://doi.org/10.1080/10623320600972051","url":null,"abstract":"To investigate whether antihemostatic function of vascular endothelial cells (VECs) is changed in type-II diabetic model rats, the mRNA expressions of tissue-type and urokinase-type plasminogen activator (t-PA and u-PA), thrombomodulin (TM), PA inhibitor type-1 (PAI-1), and phosphodiesterases (type 3A, 3B, and 4D PDEs) were quantitated by the method of comparative reverse transcriptase-polymerase chain reaction (RT-PCR). VECs from type-II diabetic model Otsuka Long-Evans Tokushima Fatty (OLETF) rats and from its normal counterpart (LETO) rats were cultured for 24 h with dibutyryl adenosine 3',5'-cyclic monophosphate (db-cAMP) or a type-3 PDE inhibitor, cilostazol. Intracellular cAMP concentration was determined by the chemiluminescent enzyme-linked immunosorbent assay (ELISA) system. In cultured VECs from OLETF rats, the basal mRNA expressions of u-PA and TM were significantly decreased as compared to those in cultured VECs from LETO rats. TM mRNA expression in cultured VECs from OLETF rats was increased 2.1-fold at 24 h after treatment with db-cAMP (3 mmol/L). Basal mRNA expressions of type 3A, 3B, and 4D PDEs were significantly higher in VECs from OLETF rats than those from LETO rats. After treatment with cilostazol (30 micromol/L), intracellular cAMP was significantly increased at 60 min and TM mRNA expression was increased 1.5-fold at 24 h. Therefore, elevation of intracellular cAMP by db-cAMP or cilostazol up-regulated TM mRNA expression in cultured VECs from OLETF rats.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320600972051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26352038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Phenotype gradation of human saphenous vein endothelial cells from cardiovascular disease subjects. 心血管疾病患者大隐静脉内皮细胞的表型分级。

Endothelium : journal of endothelial cell research Pub Date : 2006-09-01 DOI: 10.1080/10623320600972036

C Krishna Prasad, K Jayakumar, Lissy K Krishnan

{"title":"Phenotype gradation of human saphenous vein endothelial cells from cardiovascular disease subjects.","authors":"C Krishna Prasad, K Jayakumar, Lissy K Krishnan","doi":"10.1080/10623320600972036","DOIUrl":"https://doi.org/10.1080/10623320600972036","url":null,"abstract":"The highly organized histological architecture of the vascular wall and the specialized cellular phenotypes are perturbed in conditions such as atherosclerosis, restenosis, and hypertension. Alterations of endothelial cell (EC) phenotype in cardiovascular diseases (CVDs) as an effect of alteration of extracellular matrix (ECM) composition have not been well understood. In vitro study of EC phenotype is limited because they tend to dedifferentiate in subcultures. The objective of this study was to use in vitro cell culture on a biomimetic matrix model to characterize phenotype of human saphenous vein endothelial cells (HSVECs) harvested from CVD patients. Parameters studied were mRNA expression and synthesis of von Willebrand factor (vWF), plasminogen activation inhibitor (PAI), tissue plasminogen activator (t-PA), and endothelial nitric oxide synthetase (eNOS). Proliferation and apoptosis of HSVEC cultures obtained from eight different patients were compared on two matrices until passage 12. In early passages, both the prothombotic molecules vWF and PAI were overexpressed, whereas the antithrombotic molecules t-PA and eNOS were underexpressed. With increase in passage number, low expression of prothrombotic molecules and high expression of antithrombotic molecules were seen in cells on the model matrix. But when cells were grown on conventional gelatin-coated polystyrene, expression of prothrombotic molecules amplified further and antithrombotic molecules lessened with the progression of passages. On the model matrix HSVECs showed good proliferation rate and survival through several passages. It is demonstrated that matrix composition can influence switching of EC phenotypes into pro/antithrombotic states. This matrix model may be suitable to study the effect of exogenous factors on EC dysfunction with respect to CVD.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320600972036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26351455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

High efficiency and long-term foreign gene expression in cultured liver sinusoidal endothelial cells by retroviral transduction. 逆转录病毒转导在培养的肝窦内皮细胞中高效、长期地表达外源基因。

Endothelium : journal of endothelial cell research Pub Date : 2006-07-01 DOI: 10.1080/10623320600904088

Jesus Paez, Ramon Montaño, Lorenzo Benatuil, John Iacomini, Jose E Cardier

{"title":"High efficiency and long-term foreign gene expression in cultured liver sinusoidal endothelial cells by retroviral transduction.","authors":"Jesus Paez, Ramon Montaño, Lorenzo Benatuil, John Iacomini, Jose E Cardier","doi":"10.1080/10623320600904088","DOIUrl":"https://doi.org/10.1080/10623320600904088","url":null,"abstract":"The liver sinusoidal endothelial cells (LSECs) constitute a very specialized endothelium. Due to their multiple functions and privileged location in the liver, these cells constitute an excellent target for gene therapy. In this work, the authors investigate the efficiency of retroviral gene transduction as a method for in vitro gene delivery into murine LSECs. Gene transduction into murine LSECs was performed using the PCMMP-eGFP/pIK-MLVgp retrovirus pseudotyped with the vesicular stomatitis virus G glycoprotein (VSV-g), containing eGFP as a reporter gene. Retroviral transduction resulted in a high efficiency of gene transfer (99%) and stable expression of eGFP in LSECs. The retroviral transduction protocol did not affect the morphology or expression of endothelial cell markers or the biological functions of LSECs. The authors have developed conditions for high-efficiency and stable retroviral gene transduction of LSECs. These results raise the possibility of liver gene therapy using LSECs as vehicle for the delivery of therapeutic proteins by means of retroviral vectors.","PeriodicalId":11587,"journal":{"name":"Endothelium : journal of endothelial cell research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2006-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10623320600904088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26265254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Role of CD7 expressed in lung microvascular endothelial cells as Fc receptor for immunoglobulin M. 肺微血管内皮细胞表达的CD7作为免疫球蛋白M Fc受体的作用。

Endothelium : journal of endothelial cell research Pub Date : 2006-07-01 DOI: 10.1080/10623320600904138

Motoko Nishimura, Minoko Takanashi, Hitoshi Okazaki, Masahiro Satake, Kazunori Nakajima

引用次数: 4